The role of bedding on the pulse shape change: Numerical modeling calibration with a physical experiment

Author(s):  
José Eduardo M. Lira ◽  
Webe J. Mansur
Author(s):  
Hideo Hayashi ◽  
Yoshikazu Hirai ◽  
John T. Penniston

Spectrin is a membrane associated protein most of which properties have been tentatively elucidated. A main role of the protein has been assumed to give a supporting structure to inside of the membrane. As reported previously, however, the isolated spectrin molecule underwent self assemble to form such as fibrous, meshwork, dispersed or aggregated arrangements depending upon the buffer suspended and was suggested to play an active role in the membrane conformational changes. In this study, the role of spectrin and actin was examined in terms of the molecular arrangements on the erythrocyte membrane surface with correlation to the functional states of the ghosts.Human erythrocyte ghosts were prepared from either freshly drawn or stocked bank blood by the method of Dodge et al with a slight modification as described before. Anti-spectrin antibody was raised against rabbit by injection of purified spectrin and partially purified.


2021 ◽  
Author(s):  
◽  
Marie Bell

<p>This thesis presents the voices of 17 pioneers of the organisation parents' Centre, founded in Wellington, New Zealand, in 1952. They reflect on Parents' Centre's contribution to the welfare and happiness of young children and their parents, and the challenges and satisfactions for them as 'movers and shakers' of an entrenched system. The pioneers, 13 women and 3 men, were a group of professionals and parents educated in the progressive tradition who worked as volunteers to found and develop the organisation. They challenged the well-established and generally respected views of the policymakers of the 1950s about the management of childbirth and parent education for young children. They believed that the education and care of the child from birth to three needed to be brought into line with the progressive principles and practices which had been gaining ground in the schools and pre-schools of New Zealand since the 1920s and which emphasised holistic development, especially the psychological aspects. Using Bronfenbrenner's ecological systems theory I set the study within the social climate of the 1950s to assess the contribution the changing times made to the success of the organisation. I identified the social and economic forces which brought change both in the institutions of society and within every day family life, particularly for young children and their parents. As researcher, I added my voice to their reflections while also playing the role of analyst. The study used an oral history method to record the stories of the participants from a contemporary perspective. My involvement in the organisation over 50 years gave me insider knowledge and a rapport with the people interviewed. Using a loosely structured interview I adopted a collegial method of data gathering. A second interview, two years after the first, informed the pioneers about my use of the interview material and gave opportunities for critical comments on my analysis. It became apparent that under the leadership of Helen Brew, parents' Centre was able to influence change. Analyses of the background of the pioneers and of the educationalists who influenced them in training, career and parenthood show that key influences on the pioneers were lecturers at Wellington and Christchurch Training Colleges and Victoria University of Wellington. The liberal thrust of these educational institutions reinforced similar philosophical elements in the child rearing practices experienced by the pioneers. Overall, the pioneers expressed satisfaction with the philosophies and practice they advocated at that time, their achievements within Parent's Centre, and pride in founding a consumer organisation effective for New Zealand conditions. They saw Parents' Centre as having helped to shape change. This study documents the strategies used by Parents' Centre to spread its message to parents, policy makers and the general public. At the end of the study the pioneers were in agreement that the change in the role of women, particularly as equal breadwinners with men, presented a challenge to the consumer and voluntary aspects of the organisation of Parents' Centre today. Some felt the organisation had lost its radical nature and was at risk of losing the consumer voice. Nonetheless, all the pioneers felt that Parents' Centre still had a part to play in providing effective ante-natal education 'by parents for parents' and a continuing role in working for change in the services in accordance with the needs of parents and children under three.</p>


Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Seock-Won Youn ◽  
Sudhahar Varadarajan ◽  
Archita Das ◽  
Ronald D McKinney ◽  
Tohru Fukai ◽  
...  

Background: Endothelial to mesenchymal transition (EndMT) is induced by inflammation and contributes to fibrosis; however, underlying mechanism is poorly understood. Cu plays an important role in physiological processes and pathophysiologies associated with inflammatory diseases. Since excess Cu is toxic, bioavailability of Cu is tightly controlled by Cu exporter ATP7A, which obtains Cu via Cu chaperone, Atox1, and exclude Cu. We reported that Atox1 also functions as a Cu dependent transcription factor. However, role of Cu transport proteins in EndMT is entirely unknown.[[Unable to Display Character: &#8232;]] Results: Here we show that TNFα stimulation for 24hr in HUVEC significantly decreased ATP7A protein (80%) and increased intracellular Cu and Atox1 in nucleus, which was associated with shape change forming EndMT. ATP7A depletion with shRNA in EC significantly reduced EC markers (VE-cadherin and VEGFR2) and increased mesenchymal markers (αSMA, Calponin, SM22α, Collagen I/II). ATP7A siRNA also increased intracellular Cu and nuclear Atox1. These ATP7A knockdown-induced phenotype changes were inhibited by Cu chelators BCS and TTM. Mechanistically, microarray and qPCR based screening revealed that ATP7A knockdown in EC significantly increased miR21 (2.5 fold) and miR125b (1.5 fold) which induce EndMT in a Cu-dependent manner. Of note, promoters of both miR21 and miR125b have Cu dependent transcription factor Atox1 binding sites. Consistent with this, overexpression of Atox1 increased miR21 and miR125b expression as well as promoted EndMT. In vivo, ATP7A mutant (ATP7Amut) mice with reduced Cu export function showed impaired blood flow recovery and reduced arteriogenesis while increased αSMA+ cells and fibrosis in capillary network after ischemic injury. Moreover, ATP7Amut mice crossed with ApoE-/- mice with high fat diet (HFD) induced robust fibrosis and enhanced atherosclerotic lesion vs ApoE-/-/HFD mice.[[Unable to Display Character: &#8232;]] Conclusions: ATP7A protects against fibrosis by preventing EndMT via nuclear Atox1-mediated upregulation of miR21 and miR125b which induce EndMT, in Cu dependent manner. These findings provide the foundation for novel protective role of Cu transport proteins against EndMT- and fibrosis-mediated cardiovascular diseases.


Blood ◽  
1992 ◽  
Vol 79 (3) ◽  
pp. 782-786 ◽  
Author(s):  
SL Schrier ◽  
A Zachowski ◽  
PF Devaux

Abstract We studied stomatocytosis induced in human red blood cells (RBC) by vinblastine and chlorpromazine, monitoring the movements of spin- labeled phosphatidylcholine (PC*) and sphingomyelin (SM*) by electron spin resonance (ESR) spectroscopy. This technique allows determination of the fraction of labeled lipids, respectively, on the external leaflet, on the cytosol face, or trapped in endocytic vacuoles. Both vinblastine and chlorpromazine produce a time- and concentration- dependent stomatocytic shape change, which is paralleled by a shift of approximately 10% to 33% of outer leaflet SM* and PC* inward. Of this amount, 8% to 12% was trapped in endocytic vacuoles and 8% to 19% had flipped to the inner leaflet. Vanadate, while inhibiting the stomatocytosis, did not block the flip of either SM* or PC* to the inner leaflet. To explain the inhibiting effect of vanadate, as well as the adenosine triphosphate (ATP) requirement for drug-induced stomatocytosis, we propose the following model: (1) addition of amphipath partially scrambles the bilayer; and (2) the flop of phosphatidylserine (PS) and phosphatidylethanolamine (PE) to the outer leaflet provides substrate for the aminophospholipid translocase (APLT), which flips back PS and PE inward faster than PC or SM can diffuse outward--thereby producing inner layer expansion or stomatocytosis. This role of APLT accounts for the vanadate inhibition of amphipath stomatocytosis. However, the vanadate effect can be overcome by increasing the amphipath concentration, which at such levels probably passively expands the inner leaflet.


2016 ◽  
Vol 825 ◽  
pp. 165-169
Author(s):  
Michael Somr ◽  
Petr Kabele

The formation of a caldera poses a serious risk for the society and the environment. There are several established processes (mostly dealing with the conditions inside the reservoir), which must take place in order to reach a collapse leading to the caldera. The role of magma chamber geometry is investigated in this paper, exploiting the numerical modeling. The results indicates that the knowledge of the magmatic system dimensions can provide a helpful factor for an assessment of the caldera formation scenario.


1989 ◽  
Vol 56 (2) ◽  
pp. 299-306 ◽  
Author(s):  
Tohru Nakano ◽  
Kohji Hanasaki ◽  
Hitoshi Arita

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