Homologous Expression and T3SS-Dependent Secretion of TAP-Tagged Xo2276 in Xanthomonas oryzae pv. oryzae Induced by Rice Leaf Extract and Its Direct In Vitro Recognition of Putative Target DNA Sequence

2013 ◽  
Vol 23 (1) ◽  
pp. 22-28 ◽  
Author(s):  
Seunghwan Kim
Keyword(s):  
Dna Sequence ◽  
Leaf Extract ◽  
Xanthomonas Oryzae ◽  
Rice Leaf ◽  
Homologous Expression ◽  
Putative Target ◽  
Target Dna

scholarly journals Efikasi Ekstrak Sirih, Rimpang Lengkuas dan Kunyit terhadap Penekanan Pertumbuhan Xanthomonas oryzae

2021 ◽  
Vol 8 (1) ◽  
pp. 53-65
Author(s):  
Rini Laraswati ◽  
Umi Kulsum ◽  
Evan Purnama Ramdan
Keyword(s):  
Leaf Extract ◽  
Petri Dish ◽  
Xanthomonas Oryzae ◽  
Control Treatment ◽  
Disease Development ◽  
Betel Leaf ◽  
Plant Pest ◽  
Tropical Areas ◽  
Inhibitory Power

One of the important diseases in rice is the Bacterial Leaf Blight (HDB) or the so-called kresek disease caused by Xanthomonas oryzae pv. oryzae. This disease is one of the main diseases of rice in Indonesia. This is supported by agricultural conditions in hot and humid tropical areas so that disease development is more optimal. The purpose of this study was to determine the efficacy of betel leaf extract and some rhizomes in suppressing the growth of Xanthomonas oryzae pv. oryzae in-vitro scale. The research was conducted at the Center for Forecasting Plant Pest Organisms (BBPOPT), Karawang, West Java. The extracts of galangal, turmeric, and betel were prepared at concentrations of 10, 15, and 25%, the method used was a scatter plate by taking 10, 50, and 100 μL of Xoo isolate liquid, holding the petri dish to a bunsen fire, and spraying Xoo isolates liquid. into a petri dish containing PSA media + rhizome extract, the dispersing tool used is drigalski. The results of daily observations of rhizome extract antagonist testing on Xoo growth showed that the treatment of betel leaf extract with a concentration of 10%, 15%, and 25% had a high bacterial inhibitory value compared to other treatments with 100% inhibition, whereas in the control treatment (only PSA media) shows that it does not have bacterial inhibition, and the galangal rhizome extract treatment has the highest inhibitory power when compared to other treatments on 100 μL of Xoo bacterial suppression.

Transcriptional expression of aminoacyl tRNA synthetase genes of Xanthomonas oryzae pv. oryzae (Xoo) on rice-leaf extract treatment and crystal structure of Xoo glutamyl-tRNA synthetase

2017 ◽  
Vol 68 (5) ◽  
pp. 434
Author(s):  
Thien-Hoang Ho ◽  
Myoung-Ki Hong ◽  
Seunghwan Kim ◽  
Jeong-Gu Kim ◽  
Jongha Lee ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Bacterial Blight ◽  
Leaf Extract ◽  
Catalytic Domain ◽  
Trna Synthetase ◽  
Binding Domain ◽  
Xanthomonas Oryzae ◽  
Transcriptional Expression ◽  
Aminoacyl Trna Synthetase ◽  
Rice Leaf

Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of bacterial blight of rice, one of the most devastating rice diseases. We analysed the time-resolved transcriptional expression of aminoacyl-tRNA synthetase (aaRS) genes in Xoo cells treated with rice-leaf extract. Most aaRS genes showed decreased expression in the initial 30 min and recovered or increased expression in the later 30 min. The protein-synthetic machinery of bacterial cells is an important target for developing antibiotic agents; aaRSs play an essential role in peptide synthesis by attaching amino acids onto the corresponding tRNA. In bacteria, glutaminyl-tRNA (Gln-tRNAGln) is synthesised in two steps by glutamyl-tRNA synthetase (GluRS) and tRNA-dependent aminotransferase, the indirect biosynthetic mechanism of which is not present in eukaryotes. We determined the crystal structure of GluRS from Xoo (XoGluRS) at resolution of 3.0 Å, this being the first GluRS structure from a plant pathogen such as Xoo. The XoGluRS structure consists of five domains, which are conserved in other bacterial GluRS structures. In the bacterial GluRS structures, the Rossmann-fold catalytic domain and the stem-contact domain are most conserved in both sequence and structure. The anticodon-binding domain 1 is less conserved in sequence but overall structure is conserved. The connective-polypeptide domain and the anticodon-binding domain 2 show various conformations in structure. The XoGluRS structure could provide useful information to develop a new pesticide against Xoo and bacterial blight.

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu
Keyword(s):  
Solanum Tuberosum ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Leaf Extract ◽  
Solanum Tuberosum L ◽  
In Vitro Toxicity ◽  
Potato Plants ◽  
Colony Diameter ◽  
In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

Assessment of in vitro antiarthritic activity of Manilkara hexandra (Roxb.) Dubard leaf extract

2016 ◽  
Vol 5 (2) ◽  
pp. 152-155
Author(s):  
Divya Pingili ◽  
Archana Awasthi ◽  
Deepa Amminbavi
Keyword(s):  
Leaf Extract
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