Effect of Solvent on the Characteristics of Electrospun Regenerated Silk Fibroin Nanofibers

2007 ◽  
pp. 813-816
Author(s):  
Lim Jeong ◽  
Kuen Yong Lee ◽  
Won Ho Park
Keyword(s):  
Silk Fibroin ◽  
Effect Of Solvent ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Nanofibers

scholarly journals Adsorption/desorption and biofunctional properties of oleuropein loaded on different types of silk fibroin matrices

2017 ◽  
Vol 36 (1) ◽  
Author(s):  
Oguz Bayraktar ◽  
Ali Bora Balta ◽  
Guldemet Basal Bayraktar
Keyword(s):  
Silk Fibroin ◽  
Antibacterial Properties ◽  
Average Diameter ◽  
Electrospinning Technique ◽  
Collector Plate ◽  
Regenerated Silk Fibroin ◽  
Different Types ◽  
Healing Property ◽  
Silk Fibroin Nanofibers ◽  
Adsorption Desorption

The objective of this study was to investigate the adsorption/desorption behavior of oleuropein on different types of silk fibroin matrices including silk fibroin microfibers (MF), regenerated silk fibroin (RSF), and silk fibroin nanofibers (NF). Nanofibers with an average diameter of ranging between 24 and 326 nm were successfully prepared using the electrospinning technique. The effects of the silk fibroin concentration, the voltage applied and the distance between needle tip and collector plate on the morphology of the NF were investigated. The adsorption capacities of MF, RSF and NF were determined as 104.92, 163.07 and 228.34 mg oleuropein per gram of material, respectively. The percentage of initially adsorbed oleuropein that was desorbed was 86.08, 91.29 and 96.67% for MF, RSF and NF, respectively.NF and RSF discs loaded with oleuropein were subjected to disc diffusion assays to determine their antibacterial activity against test microorganisms Staphylococcus epidermidis (Gram +) and Escherichia coli (Gram – ). The results showed that both biomaterials possessed antibacterial properties after loading with oleuropein. Wound scratch assays using oleuropein released from NF revealed an enhancement of cell migration, indicating a wound healing property of the material.In conclusion, the NF can be utilized as a biofunctional polymeric material with better performance for the adsorption and desorption of oleuropein compared with MF and RSF.

Effect of Solvent on the Characteristics of Electrospun Regenerated Silk Fibroin Nanofibers

2007 ◽  
Vol 342-343 ◽  
pp. 813-816 ◽  
Author(s):  
Lim Jeong ◽  
Kuen Yong Lee ◽  
Won Ho Park
Keyword(s):  
Secondary Structure ◽  
Formic Acid ◽  
Silk Fibroin ◽  
Structural Changes ◽  
Effective Means ◽  
Electrospun Nanofibers ◽  
Random Coil ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Nanofibers ◽  
Β Sheet

Nonwoven nanofiber matrices were prepared by electrospinning a solution of silk fibroin (SF) dissolved either in formic acid or in 1,1,1,3,3,3-hexafluoro-2-isopropyl alcohol (HFIP). The mean diameter of the electrospun nanofibers prepared from SF dissolved in formic acid was 80 nm with a unimodal size distribution, which was smaller than those prepared from HFIP (380 nm). SF nanofibers were then treated with an aqueous methanol solution, and structural changes due to solvent-induced crystallization of SF were investigated using IR and 13C solid-state CP/MAS NMR spectroscopy. SF nanofibers prepared from formic acid were found to have a higher proportion of β-sheet conformations than those prepared from HFIP. Methanol treatment provided a fast and effective means to alter the secondary structure of both types of SF nanofibers from a random coil form to a β-sheet form. As demonstrated in the present study, this approach to controlling the dimensions and secondary structure of proteins using various solvents may be useful for the design and tailoring of materials for biomedical applications, especially for tissue engineering applications.

scholarly journals Effect of Sodium Carbonate Concentrations on the Formation and Mechanism of Regenerated Silk Fibroin Nanofibers by Electrospinning

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Hao Dou ◽  
Baoqi Zuo
Keyword(s):  
Rheological Property ◽  
Aqueous Solutions ◽  
Silk Fibroin ◽  
Sodium Carbonate ◽  
Electrospinning Process ◽  
Carbonate Concentration ◽  
Low Viscosity ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Nanofibers ◽  
First Time

Degumming is the first process for the preparation of all silk-based products. In this paper, effect of sodium carbonate concentrations for silk degumming on the formation of electrospun silk fibroin nanofibers was investigated and the reason for the silk electrospinning process was explained for the first time by differences from the microstructure of regenerated silk fibroin. With increasing the sodium carbonate concentration, microstructure both in the aqueous solutions and in the electrospinning solutions transformed from nanofibrils to nanoparticles, leading to obvious changes on rheological property; electrospinning solutions with nanofibrils behaved like the native silk dope and owned remarkably higher viscosity than the solutions with nanoparticles showing very low viscosity. More interestingly, nanofibrils favored the formation of silk nanofibers with ease, and even nanofibers could be electrospun at concentration 2%. However, nanoparticles were completely unable to generate nanofibers at high spinning concentration 8%. Importance of sodium carbonate concentrations is heavily emphasized for impacting the microstructure types and further influencing the electrospinning performance of regenerated silk. Hence, sodium carbonate concentrations provide a controllable choice for the preparation of silk-based electrospun biomaterials with desired properties.

Property Research of Silk Fibroin Nanofibers by Electrospinning Dissolved in CaCl2-Formid Acid

2015 ◽  
Vol 1120-1121 ◽  
pp. 331-336
Author(s):  
Yan Zhou ◽  
Hui Ying Wu
Keyword(s):  
Mechanical Properties ◽  
Silk Fibroin ◽  
Solvent System ◽  
Dissolution Time ◽  
Ethanol Treatment ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroin Nanofibers ◽  
Different Content ◽  
Β Sheet ◽  
Paper Property

In this paper, property research of silk fibroin nanofibers by electrospinning dissolved in CaCl2-Formid Acid was discussed. The dissolve process, morphology, structure and mechanical properties of fibers were studied. It showed that more dissolution time was needed to dissolve silk with 1% CaCl2. The diameters of silk fibroin nanofibers decreased with the CaCl2 contents increased and the surface of 6%5% fibers was more uniform and smooth. After ethanol treatment, strong peak at 1628cm-1, 1517cm-1 and 1231cm-1 was corresponding to β-sheet. It was important to discover that silk fibroin was weakly destroyed in CaCl2-FA solvent system, and the regenerated silk fibroin nanofibers had better mechanical properties. The stress of 6%5% fibers was nearly 3 times to traditional way. With different content of CaCl2, the stress of 6%5% fibers was nearly 2 times to 6%1% fibers.

Ethanol Vapor-Induced Morphology and Structure Change of Silk Fibroin Nanofibers

2010 ◽  
Vol 160-162 ◽  
pp. 1165-1169 ◽  
Author(s):  
Lin Peng Fan ◽  
Zeng Xiao Cai ◽  
Chun Chen Wu ◽  
Xiao Hua Geng ◽  
Hong Sheng Wang ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Ethanol Vapor ◽  
Structure Change ◽  
Attractive Alternative ◽  
Scanning Calorimetry ◽  
Atomic Force ◽  
Regenerated Silk Fibroin ◽  
Morphology And Structure ◽  
Nanofibrous Membranes ◽  
Silk Fibroin Nanofibers

In this study, regenerated silk fibroin (RSF, from Bombyx mori) nanofibers with smooth surface had been successfully prepared via electrospinning, as shown by SEM and then as-spun fibers were induced under 75% ethanol vapor. We aimed to investigate the morphology and structure change of 75% ethanol vapor-induced silk fibroin nanofibers. To determine any difference in surface topographies, the nanofibers were inspected using atomic force microscope (AFM) and the results showed that after inducement of 75% ethanol vapor for 24 h, the surface of fibers became rough. Differential Scanning Calorimetry (DSC) analysis indicated that electrospun SF nanofibrous membranes typically took silk I form and 75% ethanol vapor-induced SF nanofibrous membranes took silk II structure. These results suggested that 75% ethanol vapor inducement could be an attractive alternative to expand the application of RSF.

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