Optimization of the Components of Medium Producing Dextran from Immobilized Cells of Leuconostoc mesenteroides

2011 ◽  
Vol 418-420 ◽  
pp. 212-216
Author(s):  
Qing Wang ◽  
Xiao Wang ◽  
Qing Song Zou ◽  
Yuan Yuan Pu ◽  
Shan Chen
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Sodium Citrate ◽  
Immobilized Cells ◽  
Maximum Yield ◽  
Experimental Designs ◽  
Perfect Agreement ◽  
Medium Components ◽  
Original Medium ◽  
Predicted Model ◽  
Central Composite

The cells of Leuconostoc mesenteroides were immobilized for dextran producing and response surface methodology based on experimental designs were applied to optimize the medium for maximum yield. Eight different medium components were examined for their significance on dextran production using Plackett-Burman factorial design. Na2HPO4, CaCl2and sodium citrate were found to have significant effect on dextran production. The combined effect of these nutrients on dextran production was studied using central composite design. The optimal concentration of variables for maximum dextran production were 0.245 g/100mL of Na2HPO4, 0.05 g/100mL of CaCl2, 0.2084 g/100mL of sodium citrate. The maximum concentration of dextran obtained at 24 h by predicted model was 1.88045 mg/mL that was in perfect agreement with the experimental determined value 1.85395 mg/mL. Dextran yield was over 376% higher as compared to original medium which gave 0.38956 mg/mL of dextran.

scholarly journals OPTIMIZING THE PROCESS OF TRANSFORMING COFFEE HUSKS INTO BIOCHAR BY MEANS OF HYDROTHERMAL CARBONIZATION

2018 ◽  
Vol 54 (4B) ◽  
pp. 138
Author(s):  
Tran Thi Hien
Keyword(s):  
Maximum Yield ◽  
Hydrothermal Carbonization ◽  
Optimal Conditions ◽  
Coffee Husk ◽  
Optimal Value ◽  
Face Centered ◽  
Composite Face ◽  
Temperature Time ◽  
Central Composite ◽  
Water Ratio

The conditions of the hydrothermal carbonization process to produce biochar from coffee husk will be optimized for maximum yield. Besides, response surface methodology (RSM) and central composite face-centered (CCF) method will be used in designing experiments. Also, the optimal value of factors such as temperature, time and biomass: water ratio which can provide a maximum yield of biochar will be worked out using Modde 5.0. As a result, the optimal conditions for maximum yield of biochar was obtained as temperature of 180 oC, 3.5 h and biomass: water ratio of 15 %. It can also be concluded that temperature has greater impact on the transformation of biochar than time and biomass: water ratio.

scholarly journals Central Composite Design and Desirability of RSM for Optimization of Biodiesel Production from Mixture of Animal Waste Fat Oil and used Cooking Oil

2020 ◽  
Vol 9 (4) ◽  
pp. 1565-1571
Keyword(s):  
Reaction Time ◽  
Sodium Hydroxide ◽  
Maximum Yield ◽  
Animal Waste ◽  
Sodium Hydroxide Concentration ◽  
Used Cooking Oil ◽  
Input Parameters ◽  
Desirability Approach ◽  
Central Composite ◽  
Waste Fat

In this work, central composite design(CCD) and desirability approach of Response surface methodology (RSM) has been used for optimization of biodiesel yield produced from mixture of animal waste fat oil and used cooking oil (AWO) in the ratio of 1:1through alkaline transesterification process. In this work, methanol quantity, reaction time and sodium hydroxide concentration are selected as input parameters and yield selected as response. The combined effect of methanol quantity, reaction time and sodium hydroxide concentration were investigated and optimized by using RSM. The second order model is generated to predict yield as a function of methanol quantity, reaction time and sodium hydroxide concentration. A statistical model predicted the maximum yield of 96.9779% at 35ml methanol quantity (% v/v of oil), 75 min. reaction time and 0.6g (% wt./v of oil) of sodium hydroxide. Experimentally, the maximum yield of 97% was obtained at the above optimized input parameters. The variation of 0.02% was observed between experimental and predicted values. In this work, an attempt has also made to use desirability approach of RSM to optimize the input parameters to predict maximum yield. Desirability approach predicts maximum yield (97.075%) at CH3OH (35.832% vol. /vol. of oil), NaOH (0.604 % wt./vol. of oil) and reaction time (79.054min.) was found for the AWO.

Foliar Analysis as a Guide to NPK Nutrition of Pineapples in the Forest Zone of Ghana

1970 ◽  
Vol 6 (4) ◽  
pp. 327-333 ◽  
Author(s):  
W. Godfrey-Sam-Aggrey
Keyword(s):  
Maximum Yield ◽  
Second Order ◽  
Fruit Yield ◽  
Foliar Analysis ◽  
Forest Zone ◽  
Fruit Length ◽  
Nutrient Levels ◽  
Central Composite ◽  
Plot Experiment ◽  
Rotatable Design

SUMMARYIn a 20 plot experiment with central composite second-order rotatable design, basal non-chlorophyllous sections of “D” leaves of Sugarloaf pineapples were sampled at harvest and analysed for N, P, K. Maximum yield of 17·8 tons per acre of good quality Sugarloaf pineapples and maximum fruit length of 27·4 cm. were associated with the nutrient combination of 36–42–32 lb/acre N P K applied at 4, 6, 11 and 12 months. The nutrient levels in the basal sections of “D” leaves corresponding to maximum fruit yield were 0·35–0·40% N; 0·04% P; 0·44% K; K/P 11·2–11·5: 1. K/P ratio for maximum fruit length was 10·4–11·5: 1. N/K ratio was associated with lodging.

scholarly journals Medium Optimization for Spore Production of a Straw-Cellulose Degrading Actinomyces Strain under Solid-State Fermentation Using Response Surface Method

2020 ◽  
Vol 12 (21) ◽  
pp. 8893
Author(s):  
Huanran Liu ◽  
Dan Zhang ◽  
Xia Zhang ◽  
Chuanzhi Zhou ◽  
Pei Zhou ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Spore Production ◽  
Steepest Ascent ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

The strains capable of degrading cellulose have attracted much interest because of their applications in straw resource utilization in solid-state fermentation (SSF). However, achieving high spore production in SSF is rarely reported. The production of spores from Streptomyces griseorubens JSD-1 was investigated in shaker-flask cultivation in this study. The optimal carbon, organic nitrogen and inorganic nitrogen sources were sucrose, yeast extract and urea, respectively. Plackett–Burman design (PBD) was adopted to determine the key medium components, and the concentration levels of three components (urea, NaCl, MgSO4·7H2O) were optimized with the steepest ascent path and central composite design (CCD), achieving 1.72 × 109 CFU/g of spore production. Under the optimal conditions (urea 2.718% w/v, NaCl 0.0697% w/v, MgSO4·7H2O 0.06956% w/v), the practical value of spore production was 1.69 × 109 CFU/g. The determination coefficient (R2) was 0.9498, which ensures an adequate credibility of the model.

Medium Optimization for the Production of Phytase by Recombinant Pichia pastoris Grown on Glycerol

2011 ◽  
Vol 9 (1) ◽  
Author(s):  
Min Liu ◽  
Gabriel Potvin ◽  
Yiru Gan ◽  
Zhanbin Huang ◽  
Zisheng Zhang
Keyword(s):  
Pichia Pastoris ◽  
Phytase Production ◽  
Medium Components ◽  
Gap Promoter ◽  
Recombinant Pichia Pastoris ◽  
Burman Design ◽  
Statistical Designs ◽  
Plackett Burman Design ◽  
Central Composite ◽  
Three Components

Based on statistical designs, minimal salts medium, commonly used for yeast cultivation, was optimized to maximize GAP promoter-mediated phytase production by recombinant Pichia pastoris grown on glycerol. A Plackett-Burman design was followed to screen medium components to determine those that significantly affected phytase production. Of the 8 components studied, the concentrations of K2SO4, CaSO4•2H2O and MgSO4•7H2O were identified as having a significant effect. These three components were subsequently optimized by response surface methodology using a central composite design. The optimal concentrations of the three components, leading to a maximal extracellular phytase activity of 161.64 U/ml, were K2SO4 13.25g/l, CaSO4•2H2O 1.03g/l and MgSO4•7H2O 17.94g/l. The activity measured in cultures using optimized growth medium is significantly higher than the 73.31 U/ml measured in cultures using standard minimal salts media. The theoretical phytase yields predicted by the developed model were very close to experimentally obtained values.

scholarly journals Media optimization for C-phycocyanin production in Plectonema sp. using response surface methodology and central composite design

2020 ◽  
Vol 11 (3) ◽  
pp. 3897-3904
Author(s):  
Arbab Husain ◽  
Fahad Khan ◽  
Khwaja Osama ◽  
Sadaf Mahfooz ◽  
Adeeba Shamim ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Citric Acid ◽  
Central Composite Design ◽  
Response Surface ◽  
Nostoc Muscorum ◽  
Therapeutic Implications ◽  
Medium Components ◽  
Anabaena Sp ◽  
Different Strains ◽  
Central Composite

Cyanobacteria represent the richest sources of phycobiliproteins with especial reference to C-phycocyanin (C-PC), which in turn holds exhaustive therapeutic implications. Screening of several cyanobacterial strains namely Anabaena sp., Nostoc muscorum, Cylindrospermum sp., Plectonema sp., Scytonema sp., Spirulina sp., Synechococcous sp. and Tolypothrix sp. was carried out for their C-PC producing capacity, however the produced quantity of C-PC varies greatly among different strains. Owing to the crucial role of different media constituents on productivity of C-PC the current study was designed to optimize most appropriate media composition for augmented CPC production by selected superior producer. 36 factorial central composite design (CCD) dependent response surface methodology (RSM) was utilized to estimate the important medium components attributed with influencing C-PC productivity. RSM analysis of five independent coded factors including Na2CO3, K2HPO4, NaNO3, citric acid and EDTA were analyzed preceded by recognition of efficient variables for algal components production by Plectonema sp. Investigation of results revealed that the eminent medium components were NaCO3 (0.4 g/L); NaNO3 (0.5 g/L); K2HPO4 (2.8 g/L); citric acid (0.08 g/L) and EDTA (0.01 g/L) respectively. The optimized combination yielded 0.5536 mg/ml of C-PC. The increment of C-PC yield is R-Sq = 88.2%. Thus, our study led to the recognition of critical nutritional component that can be used further for enhanced productivity of C-PC.

scholarly journals TOLUENE DEGRADATION BY FREE STAPHYLOCOCCUS GALLINARUM AND IMMOBILIZED ON MULTI-WALLED CARBON NANOTUBES

2021 ◽  
Vol 10 (1) ◽  
pp. 61-73
Author(s):  
Fatemeh Heydarnezhad ◽  
Mehran Hoodaji ◽  
Mahdi Shahriarinour ◽  
Arezoo Tahmourespour
Keyword(s):  
Carbon Nanotubes ◽  
Immobilized Cells ◽  
Toluene Degradation ◽  
Toluene Concentration ◽  
Degrading Bacterium ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes ◽  
Optimized Conditions ◽  
Central Composite ◽  
Staphylococcus Gallinarum

Hydrocarbons pollution is a most important environmental and healthanxiety . Using free and immobilized bacteria could be a suitable attitude to find a proper bioaugmentation agent. A toluene degrading bacterium was isolated from oil-contaminated environs (located in Bandar-Anzali, Guilan, Iran). The strain was molecularly identified as Staphylococcus gallinarum ATHH41 (Accession number: KX344723) by partial sequencing of 16SrDNA gene. The response surface methodology (RSM) was expended for biodegradation of the toluene by ATHH41. The central composite design (CCD) was utilized to optimize pH, temperature, and toluene concentration by ATHH41. In accordance with the optimization purpose of the Design-Expert software, the optimum circumstances of toluene degradation were obtained when pH, temperature and toluene concentration were adjusted to 7.68, 31.73°C and 630.04 mg.l-1, respectively. Multi-walled carbon nanotubes (MWCNTs) were used to immobilize the strain. Infrared spectroscopy and scanning electron microscopy showed that the cells adhered to the MWCNT surface and developed a biofilm. Results reveal that free cells were able to degrade 68.01% of the toluene as the sole carbon and energy source within 24 h under optimized conditions. The immobilized cells reached 95.68%.

scholarly journals Statistical Optimization of Medium Components of Xylanase Production by Irpex lacteus BAFC 1168 strain F under liquid fermentation

2015 ◽  
Vol 5 (1) ◽  
pp. 559-569 ◽  
Author(s):  
Gabriela Verónica Díaz
Keyword(s):  
Central Composite Design ◽  
Xylanase Activity ◽  
White Rot ◽  
White Rot Fungus ◽  
Irpex Lacteus ◽  
Xylanase Production ◽  
Liquid Fermentation ◽  
Medium Components ◽  
The Media ◽  
Central Composite

The enhanced xylanase production of white rot fungus Irpex lacteus BAFC 1168 strain F using a liquid fermentation culture was investigated. The media nutrients were screened by a Plackett-Burman design and from the analysis of variance, carboxy-methylcellulose, urea and peptone were found to be the most positive and significant nutrient components. Hence, these three variables were selected for further optimization using a five-level central composite design in response surface methodology. The validation of the model experiment was conducted reaching 8.29 U/mL of xylanase activity when the composition medium was 1.6 g/L of CMC, 0.84 g/L of urea and 2 g/L of peptone. Optimum temperature and pH conditions on xylanase activity were also determined using a central composite design. It was reached a maximum xylanase activity of 8.36 U/mL when temperature and pH were 42.8°C and 5.6, respectively.

scholarly journals Utilization of agro-industrial by-products as substrates for dextransucrase production by leuconostoc mesenteroides T3: Process optimization using response surface methodology

2021 ◽  
pp. 15-15
Author(s):  
Miona Miljkovic ◽  
Sladjana Davidovic ◽  
Aleksandra Djukic-Vukovic ◽  
Mila Ilic ◽  
Milica Simovic ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Leuconostoc Mesenteroides ◽  
Sucrose Concentration ◽  
Manganese Concentration ◽  
Application Range ◽  
Linear Effect ◽  
Central Composite ◽  
By Products ◽  
Environmental Relevance

Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme is associated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroides T3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391?0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30 % sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.

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