Optimization of Enzymatic Skinning of Catfish Nuggets Using Response Surface Methodology

2012 ◽  
Vol 472-475 ◽  
pp. 3094-3103
Author(s):  
Pramuk Parakulsuksatid ◽  
Dja Shin Wang ◽  
Juan L. Silva ◽  
Tae Jo Kim ◽  
Tong Yuan Koo
Keyword(s):  
Response Surface Methodology ◽  
Central Composite Design ◽  
Response Surface ◽  
Maximum Yield ◽  
Enzyme Concentration ◽  
Process Conditions ◽  
Peritoneal Membrane ◽  
Food Industries ◽  
Central Composite

Abstract. Enzyme utilization today constitutes an important and essential part of processes used by the modern food industries. The objective of this research was to investigate the enzymatic skinning of catfish nuggets optimized by response surface methodology to remove the peritoneal membrane of nuggets. The effect of enzyme concentration, temperature, and time was carried out using central composite design. The response surface methodology found out that the optimized process conditions for good skinning and maximum yield were the following: (a) any enzyme concentration, temperature 42°C, time 12 min; (b) 0.15% enzyme concentration, temperature 35°C and time 12 min. This finding is important for maximize enzyme utilization on manufacture processes used by the catfish industries.

scholarly journals Optimization of extraction conditions of flavonoids from Houttuynia cordata Thumb leaves

2021 ◽  
Vol 20 (02) ◽  
pp. 51-61
Author(s):  
Anh T. Vu
Keyword(s):  
Response Surface Methodology ◽  
Central Composite Design ◽  
Response Surface ◽  
Dry Matter ◽  
Raw Materials ◽  
Enzyme Concentration ◽  
Houttuynia Cordata ◽  
Independent Variables ◽  
Cellulase Enzyme ◽  
Central Composite

This study was conducted to investigate the application of cellulase enzyme in the extraction of flavonoids from Houttuynia cordata Thumb leaves and optimize the extraction conditions. Independent variables, including enzyme concentration (25 - 100 µg/mL), temperature (30 - 50oC), time (30 - 120 min) and ratio of raw materials to enzyme (1:15 - 1:30 g/mL), were investigated. Extraction conditions of flavonoids were designed according to Central Composite Design - Uniform Precision (CCD), a response surface methodology using a software JMP Pro version 13. The results indicated that the optimal extraction conditions were found to be enzyme concentration (78.0 µg/mL), temperature (41oC), time (90 min), and the ratio of material to the enzyme (1:26 g/mL). Under such conditions, the highest content of flavonoids (24.04 ± 0.05 mg/g, dry matter) was obtained and validated. It can be concluded that the flavonoids can be optimally extracted under the optimal extraction conditions with assistance of cellulase.

Enzyme-assisted Extraction for Optimized Recovery of Phenolic Bioactives from Peganum hermala Leaves Using Response Surface Methodology

2018 ◽  
Vol 17 (4) ◽  
pp. 349-354
Author(s):  
Qadir Rahman ◽  
Anwar Farooq ◽  
Amjad Gilani Mazhar ◽  
Nadeem Yaqoob Muhammad ◽  
Ahmad Mukhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzyme Concentration ◽  
Coumaric Acid ◽  
Assisted Extraction ◽  
Pre Treatment ◽  
Optimized Conditions ◽  
Enzyme Complexes ◽  
Hydrolysis Of ◽  
Central Composite

This study investigates the effect of enzyme formulations (Zympex-014, Kemzyme dry-plus and Natuzyme) on recovery of phenolics from Peganum hermala (harmal) leaves, under optimized conditions using response surface methodology. As compared to the other enzyme complexes, the yield (34 g/100g) obtained through Zympex-014-assisted extraction was higher under optimized conditions such as time (75 min), temperature (70°C), pH (6.5) and enzyme concentration (5 g/100 g) using central composite design (CCD). Effectiveness of Zympex-014 towards hydrolysis of P. hermala leaves cell wall was examined by analyzing the control and enzyme-treated leave residues using scanning electron microscope (SEM). GC/MS characterization authenticated the presence of quercetin (1.44), gallic acid (0.23), caffeic acid (0.04), cinnamic acid (0.05), m-coumaric acid (0.23) and p-coumaric acid (0.37 μg/g) as the potent phenolics in Zympex-014 based extract. It can be concluded from the findings of the current work that pre-treatment of P. hermala leaves with Zympex-014 significantly enhanced the recovery of phenolics that supports its potential uses in the nutra-pharamaceutical industry.

scholarly journals Optimization of Eugenol Extraction from Clove Oil using Response Surface Methodology

2015 ◽  
Vol 9 (11) ◽  
pp. 68
Author(s):  
Widayat Widayat ◽  
Hadiyanto Hadiyanto ◽  
Bambang Cahyono ◽  
Ngadiwiyana Ngadiwiyana
Keyword(s):  
Response Surface Methodology ◽  
Optimum Condition ◽  
Central Composite Design ◽  
Response Surface ◽  
Design Method ◽  
Main Process ◽  
Clove Oil ◽  
Design Variables ◽  
Central Composite

The objective of this research was to obtain optimum condition of eugenol production from clove oil using a central composite design method. The main process occured in the eugenol production was saphonification and neutralization processes. In order to optimize these processes, the ratio of NaOH/clove oil and temperature were studied as design variables i.e. ratio of NaOH/clove oil=1:2.5-1:3.5 while temperature was varied between 40 and 60oC. The yield of eugenol was considered as the main response in of this experiment. The result showed that the optimum condition was achieved when the temperature and the ratio of NaOH/clove oil were 50oC and 2.75:1, respectively and the yield was 39.17%.<br />

scholarly journals Pembuatan Biodiesel dari Minyak Sawit Off-Grade Menggunakan Katalis CaO Melalui Proses Dua Tahap

2015 ◽  
Vol 10 (3) ◽  
Author(s):  
Febrina Dwi Putri ◽  
Zuchra Helwani ◽  
Drastinawati Drastinawati
Keyword(s):  
Response Surface Methodology ◽  
Central Composite Design ◽  
Response Surface ◽  
Central Composite

Biodiesel merupakan sumber bahan bakar alternatif yang bisa dihasilkan dari minyak nabati melalui transesterifikasi dengan metanol. Sawit Off-grade merupakan salah satu sumber minyak nabati yang mempunyai potensi untuk digunakan sebagai bahan dasar produksi biodiesel. Reaksi transesterifikasi dilangsungkan dengan bantuan katalis CaO dari cangkang telur yang dikalsinasi pada suhu 900ºC selama 2 jam. Tujuan dari penelitian ini adalah untuk memproduksi biodiesel dari minyak sawit off-grade melalui reaksi dua-tahap, melihat performa katalis Cao terhadap reaksi transesterifikasi, serta mempelajari pengaruh temperatur reaksi, rasio mol minyak:metanol dan konsentrasi katalis terhadap yield biodiesel pada reaksi transesterifikasi. Esterifikasi dilakukan pada suhu 60°C dengan perbandingan mol minyak:metanol 1:12 menggunakan 1%wt katalis H2SO4. Asam lemak bebas (ALB) tereduksi dari 16,18% menjadi 0,6%. Reaksi transesterifikasi dilakukan dengan variasi perbandingan mol minyak:metanol 1:7, 1:9, 1:11, konsentrasi katalis 2%wt, 4%wt, 6%wt, serta suhu reaksi pada 50ºC, 60ºC, 70ºC selama 2 jam. Data yang diperoleh diproses dengan menggunakan response surface methodology (RSM), sedangkan jumlah eksperimen ditentukan dengan central composite design (CCD). Yield yang diperoleh pada penelitian ini yaitu sebanyak 62,30%-87,41%. Yield optimum dihasilkan pada reaksi dengan suhu 70ºC menggunakan minyak:metanol dengan perbandingan 1:11 dan katalis CaO 2%wt. Kondisi proses yang berpengaruh terhadap yield biodiesel adalah suhu reaksi, perbandingan mol minyak:metanol, serta interaksi antara suhu dengan perbandingan mol minyak:metanol. Akan tetapi tidak ditemukan adanya pengaruh signifikan konsentrasi katalis terhadap yield biodiesel.

scholarly journals OPTIMASI TEKSTUR BUAH TOMAT SETELAH SIMULASI TRANSPORTASI MENGGUNAKAN RESPON SURFACE METODOLOGY (RSM)

2019 ◽  
Vol 7 (2) ◽  
pp. 139-147
Author(s):  
Ida Ayu Widhiantari ◽  
Sandra Sandra ◽  
Gunomo Djoyowasito
Keyword(s):  
Response Surface Methodology ◽  
Central Composite Design ◽  
Response Surface ◽  
Central Composite

Selama kegiatan transportasi buah tomat dapat mengalami kerusakan akibat adanya frekuensi getaran yang berasal dari mesin transportasi dan juga lama waktu kegiatan transportasi. Adanya getaran selama proses transportasi buah tomat dapat mempengaruhi perubahan tekstur buah tomat. Optimasi dilakukan dalam penelitian ini dengan tujuan untuk memperoleh mutu buah tomat yang optimal setelah dilakukan simulasi transportasi. Optimasi dilakukan dengan menggunakan Response Surface Methodology (RSM) central composite design (CCD) dengan dua variabel bebas yaitu frekuensi getaran (3 Hz, 6HZ, dan 9Hz) dan lama waktu penggetaran (120, 150, dan 180 menit) serta tekstur buah tomat sebagai respon. Kondisi optimum diperoleh pada frekuensi getaran  2,33 Hz dan waktu simulasi transportasi 133,42 menit dengan nilai tekstur optimum buah tomat yaitu 0,147768 dengan nilai desirability sebesar 0,928.    

Optimization of a Whole-Stalk Operating System after Sugarcane Base Cutting

2019 ◽  
Vol 62 (1) ◽  
pp. 157-166
Author(s):  
Luxin Xie ◽  
Jun Wang ◽  
Shaoming Cheng ◽  
Jinbing Hu
Keyword(s):  
Operating System ◽  
Response Surface Methodology ◽  
Central Composite Design ◽  
Response Surface ◽  
Fracture Rate ◽  
Cleaning Performance ◽  
Abrasion Rate ◽  
Leaf Stripping ◽  
Predicted Values ◽  
Central Composite

Abstract. Leaf stripping and top breaking are the key procedures in whole-stalk sugarcane harvesting. In this study, a whole-stalk operating system after sugarcane base cutting was developed and tested to improve the leafy trash cleaning performance and adaptability of a whole-stalk harvester. A leaf shredding procedure was proposed before leaf stripping, and an anti-float top breaking device was developed for breaking cane tops. The rotational speeds of the leaf shredding (RL), leaf stripping (RS), and top breaking (RT) rollers were regarded as experimental factors, and the leafy trash content (including leaves, leaf sheaths, and tops in this study), top breaking rate, abrasion rate, and non-fracture rate of the middle or bottom parts were selected as indices. Central composite design combined with response surface methodology and comprehensive evaluation method were employed to conduct experiments and explore the interaction effects of factors on indices. The optimal operating parameters were nonlinearly optimized and determined to be RL of 512.9 rpm, RS of 418.8 rpm, and RT of 307.0 rpm. Under these conditions, the predicted values of leafy trash content, top breaking rate, abrasion rate, and non-fracture rate were 4.98%, 88.39%, 5.19%, and 96.21%, respectively. Verification experiments indicated that the observed values were in agreement with the predicted values. Results suggested that the leaf shredding device and anti-float top breaking device developed in this study were effective for improving the leafy trash cleaning performance and adaptability of the whole-stalk operating system, and there was a good potential for performance improvement of whole-stalk sugarcane harvesters. Keywords: Central composite design, Leaf shredding, Leaf stripping, Response surface methodology, Top breaking, Whole-stalk harvester.

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