Combined Effects between Functionalized Multi-Walled Carbon Nanotubes and Cigarette Smoke on Human Bronchial Epithelial Cells

2012 ◽  
Vol 486 ◽  
pp. 394-399 ◽  
Author(s):  
Xi Kai Wang ◽  
Bing Ye ◽  
Yan Qiu Zhang ◽  
Yan Yun Fu ◽  
Tao Zhang ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Dna Damage ◽  
Cigarette Smoke ◽  
Control Group ◽  
Target Cells ◽  
Significant Difference ◽  
Multi Walled Carbon Nanotubes ◽  
Cytotoxicity Effects ◽  
Walled Carbon Nanotubes ◽  
Dose Dependent

To evaluate the combined cytotoxicity effects between functionalized multi-walled carbon nanotubes (MWCNTs-PC) and cigarette smoke solution (CSS), 16-HBE cells was used as the target cells and exposed to various concentrations of MWCNTs-PC and CSS combined together. Cell proliferation, cell apoptosis, DNA damage were detected by Methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, single cell gel electrophoresis assay (SCGE) and micronuclear assay, respectively. The dose-dependent cytotoxic and genetic effects of CSS were found in our study. However, compared to the control group, the MWCNTs-PC exposed groups showed no significant difference in all concentration, with or without CSS exposure. It suggests that the MWCNTs-PC did not influence cellular toxicity or DNA damage of CSS on 16-HBE cells. No combined cytotoxic effects between NWCNTs-PC and CSS were found in this study.

scholarly journals Profiling of Sub-Lethal in Vitro Effects of Multi-Walled Carbon Nanotubes Reveals Changes in Chemokines and Chemokine Receptors

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 883 ◽  
Author(s):  
Sandeep Keshavan ◽  
Fernando Torres Andón ◽  
Audrey Gallud ◽  
Wei Chen ◽  
Knut Reinert ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Chemokine Receptors ◽  
Research Centre ◽  
Target Cells ◽  
Joint Research ◽  
Chemokine Signaling ◽  
Multi Walled Carbon Nanotubes ◽  
Downstream Analysis ◽  
Walled Carbon Nanotubes

Engineered nanomaterials are potentially very useful for a variety of applications, but studies are needed to ascertain whether these materials pose a risk to human health. Here, we studied three benchmark nanomaterials (Ag nanoparticles, TiO2 nanoparticles, and multi-walled carbon nanotubes, MWCNTs) procured from the nanomaterial repository at the Joint Research Centre of the European Commission. Having established a sub-lethal concentration of these materials using two human cell lines representative of the immune system and the lungs, respectively, we performed RNA sequencing of the macrophage-like cell line after exposure for 6, 12, and 24 h. Downstream analysis of the transcriptomics data revealed significant effects on chemokine signaling pathways. CCR2 was identified as the most significantly upregulated gene in MWCNT-exposed cells. Using multiplex assays to evaluate cytokine and chemokine secretion, we could show significant effects of MWCNTs on several chemokines, including CCL2, a ligand of CCR2. The results demonstrate the importance of evaluating sub-lethal concentrations of nanomaterials in relevant target cells.

scholarly journals Short term exposure to multi-walled carbon nanotubes induce oxidative stress and DNA damage in Xenopus laevis tadpoles

2014 ◽  
Vol 107 ◽  
pp. 22-29 ◽  
Author(s):  
Rayenne Saria ◽  
Florence Mouchet ◽  
Annie Perrault ◽  
Emmanuel Flahaut ◽  
Christophe Laplanche ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Nanotubes ◽  
Dna Damage ◽  
Xenopus Laevis ◽  
Short Term ◽  
Short Term Exposure ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Multi-walled carbon nanotubes (MWCNT): Induction of DNA damage in plant and mammalian cells

2011 ◽  
Vol 197 ◽  
pp. 327-336 ◽  
Author(s):  
Manosij Ghosh ◽  
Anirban Chakraborty ◽  
Maumita Bandyopadhyay ◽  
Anita Mukherjee
Keyword(s):  
Carbon Nanotubes ◽  
Dna Damage ◽  
Mammalian Cells ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Assessment of Human Lung Macrophages After Exposure to Multi-Walled Carbon Nanotubes Part II. DNA Damage

2011 ◽  
Vol 3 (1) ◽  
pp. 94-98 ◽  
Author(s):  
Lin Zhu ◽  
Amanda M. Schrand ◽  
Andrey A. Voevodin ◽  
Dong Wook Chang ◽  
Liming Dai ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Dna Damage ◽  
Human Lung ◽  
Lung Macrophages ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

scholarly journals Characteristics and Properties of Epoxy/Polysulfide Composite Materials Reinforced by Carbon Nanotubes

2014 ◽  
Vol 7 (3) ◽  
pp. 106-009
Author(s):  
Adnan Nemaa ◽  
Ibtihal Abed Al-Razaq ◽  
Ekhlas Edan Kader
Keyword(s):  
Electron Microscopy ◽  
Carbon Nanotubes ◽  
Fracture Strain ◽  
Wear Behavior ◽  
Structure And Morphology ◽  
Significant Difference ◽  
Multi Walled Carbon Nanotubes ◽  
Curing Agents ◽  
Field Emission Electron Microscopy ◽  
Walled Carbon Nanotubes

In this research, multi-walled carbon nanotubes (MWCNTs) were used to enforce the blend of epoxy /polysulfide and then tensile and wear behavior of this reinforcement were evaluated. For achieving this goal, different weight percentages of MWCNT (0.2–0.6 wt %) were dispersed in the epoxy resin then polysulfide resin is added and mixed with two curing agents. Experimental results have shown significant difference between epoxy/polysulfide and CNT /epoxy /polysulfide in mechanical properties. With 0.2–0.6% MWCNTs we observed an increase in Young’s modulus from 245 to273 MPa, tensile strength from 30.5 to 38.9 MPa and fracture strain from 12.4% to 14.2%. For understanding the structure and morphology of nanocomposite, the dispersion states were studied using scanning electron microscopy (SEM) and field emission electron microscopy (FESEM).

DNA damage and cytotoxicity in human bronchial normal cells (BEAS-2B) exposed to multi-walled carbon nanotubes (MWCNTs)

2010 ◽  
Vol 196 ◽  
pp. S283-S284
Author(s):  
D. Cavallo ◽  
C.L. Ursini ◽  
A. Ciervo ◽  
A.M. Fresegna ◽  
R. Maiello ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Dna Damage ◽  
Normal Cells ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes
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