Isolation and Identification of Nitrobacteria Adapted to Low Temperature

2012 ◽  
Vol 518-523 ◽  
pp. 406-410 ◽  
Author(s):  
Yun Hao ◽  
Xiu Guang Jiang ◽  
Qing Tian ◽  
Ai Yin Chen ◽  
Bao Ling Ma

In this study, a scientific method which can be used to improve nitrification process at low temperature in the sewage treatment plant was introduced. The activated sludge samples were taken from aeration tank of the sewage treatment plant when the outside temperature was below 0°C (water temperature below 12 °C). Five kinds of nitrobacteria strains with cold-resistance and higher activity of ammonia degradation were isolated from aeration tanks. The physiological properties showed the five strains were identified into Sphingobacteriaceae、Rhodanobacter sp.、Pseudomonas sp.、Pandoraea sp. and Perlucidibaca piscinae. All of the strains could convert ammonia-nitrogen or NO2- into NO3- in the medium at 10°C. The ammonia and nitrate removal efficiency could be reached 80.9% and 80.3% respectively. Comparing to the unvaccinated one, the removal efficiency can be increased by 50%, which proved the isolated nitrobacteria could be applied to biological nitrification process of sewage treatment at low-temperature.

1993 ◽  
Vol 27 (5-6) ◽  
pp. 405-412 ◽  
Author(s):  
B. Chambers ◽  
J. Whitaker ◽  
A. F. Elvidge

In the UK there are over 7000 small works which treat the sewage from populations of less than 10,000. Many of these works are at risk of non-compliance with effluent quality consents and options for improving the standard of treatment are being pursued by many utilities. WRc and Anglian Water Services have developed designs for packaged sewage treatment plants to serve populations in the range of 1000-10,000. A demonstration plant has been constructed at the Waterbeach STW of Anglian Water to serve a population of about 6,500. Target effluent quality is 15:20:5mg/l of BOD, SS and ammonia nitrogen respectively on a 95 percentile basis. Following plant commissioning a process performance evaluation programme was commenced in February 1991. Nitrification was established after about 6 weeks of operation but suspended solids values have been affected by the presence of a stable foam on the surface of the aeration tank. Process modifications have reduced the effect of this phenomenon substantially and effluent quality has improved.


1992 ◽  
Vol 25 (4-5) ◽  
pp. 233-240
Author(s):  
T. Palmgren

Due to the slow growth of nitrification bacteria at low temperatures, nitrogen reduction normally requires long hydraulic retention time during winter. Important for the nitrification process is the aerated sludge age. Factors influencing the sludge age are aerated volume, mixed liquor suspended solids concentration, organic loading and sludge yield. In an existing plant you cannot easily expand the volume and the load is difficult to decrease. But the suspended solids concentration can be increased by running the biological step with the contact stabilisation process. At the Käppala Association sewage treatment plant in Lidingö just outside Stockholm, one of the six aeration tanks has been reconstructed for full scale nitrogen removal experiments. In this tank the old aeration system has been replaced with rubber membrane diffusers. Further more there are several zones separated by walls in the tank. The tank can thereby be run with great flexibility. By running it with the contact stabilisation process, the sludge age has been improved by a factor between 1.5 and 2 and thereby it succeeds in keeping the nitrification bacteria in the system even during snow melting. At temperatures of about 9 °C and hydraulic retention times of less than 3 hours in the contact zone there has been a nitrification degree of up to 50 to 60 %. The experiment was conducted with a stabilisation zone of up to half the total volume of the aeration tank. The main purpose for the experiments during the winter seasons was to improve nitrification. Keeping the nitrifiers in the system had been a crucial problem during previous years. When the nitrifiers were lost with an increased flow and decreased temperature the nitrification process didn't restart until the temperature was increased and the load decreased. Usually this didn't occur until the middle of the summer meaning a loss of nitrification for up to six months. In Sweden there is a goal set for 50 % nitrogen reduction for the plants in the Stockholm region. At Käppala we manage to keep 60 to 70 % nitrogen reduction during the warm season, that is from July to December. If we can keep up the nitrification the whole year we can achieve 50 % as a yearly average under normal conditions even though we can't keep the nitrogen reduction rate as high during the cold season.


Author(s):  
Paulo Fortes Neto ◽  
Nara Lucia Perondi Fortes ◽  
Elizabeth Da Costa Neves Fernandes de Almeida Duarte ◽  
Rita Do Amaral Fragoso ◽  
Ana Catarina Marcos Henriques ◽  
...  

  The study reports the performance of a sanitary effluent treatment constituted by a septic tank, anaerobic filter and constructed wetland. The study monitored nutrient’s, carbonaceous material’s and thermotolerant coliform’s (CT) removal efficiency during 12 months. The treatment system included a septic tank, an anaerobic filter and a horizontal subsurface flow constructed wetland cultivated with Typha spp. Effluent samples were monthly collected before and after the septic tank, anaerobic filter and wetland. The removal efficiency for N-NH+4 was 37.6%, 66.3% for total P, 37% for COD, 54% for BOD and 99.4% for CT. The anaerobic filter and wetland were more efficient than the septic tank. P-total reduction was higher in the constructed wetland than in the anaerobic filter. Climatic conditions influenced the evaluated constituent’s removal being the highest values during hot months.


2013 ◽  
Vol 141 (12) ◽  
pp. 2658-2662 ◽  
Author(s):  
C. KLEMENT ◽  
R. KISSOVA ◽  
V. LENGYELOVA ◽  
D. STIPALOVA ◽  
Z. SOBOTOVA ◽  
...  

SUMMARYWe report the outcome of an 11-year programme monitoring sewage water and acute flaccid paralysis (AFP) cases as part of the World Health Organization (WHO) strategy for polio eradication in the Slovak Republic (SR). Polioviruses (PV) and non-polio enteroviruses (NPEV), prior to and after the change in polio vaccination strategy, were detected. Sewage treatment plant samples from 48 localities spread over the Western, Central and Eastern regions and clinical material from AFP cases were examined. The WHO standard procedures were followed with regard to virus isolation and identification. There were 538 commonly detected human enteroviruses (HEVs) including 213 (40%) coxsackie B viruses (CBV), 200 (37%) echoviruses and 113 (21%) Sabin-like PVs (PV1, 2, 3) including vaccine-derived poliovirus (VDPV) isolates. The percentage of PV isolates fell from 66% to 30% during 2001–2005 and thereafter fell to zero. CBV5, CBV2 and echovirus 3 were the NPEVs endemic during the study period.


2011 ◽  
Vol 281 ◽  
pp. 309-312
Author(s):  
Ying Zi Lin ◽  
Chang Hu Cao ◽  
Jun Yin ◽  
Wu Chao

For low-temperature conditions, the municipal sewage plant on the low removal efficiency of endocrine disrupting chemicals, We are the investigation of endocrine disrupting chemicals in a sewage plant of Changchun City. The results showed that the water contains contaminants 2,4,6-Tris[Bis(Methoxymethyl)Amino]-1,3,5-Triazine, the substance is endocrine disruptors chemicals, it is more harmful to the environment. In addition, Out of the water contains Triethylphosphate, it is the metabolites of organ phosphorus pesticides, it also has a greater impact on the environment, they should be targeted pollutants, focusing on removal of sewage under low temperature conditions for the effective removal of EDCs to provide theoretical guidance.


1988 ◽  
Vol 20 (4-5) ◽  
pp. 85-92 ◽  
Author(s):  
L.-G. Reinius ◽  
J. Hultgren

Henriksdal sewage treatment plant is the largest plant in Stockholm with a design flow of 370 000 m3/d. In one aeration tank of eleven a new fine-bubble aeration system has been in operation since August 1985. The tank is divided into 6 equal parts. The first part is an anoxic zone and the other five are aeration zones with tapered diffusers. Several instruments are installed in the block including separate air flow monitors in each of the five zones and D.O.-probes in the inlet and outlet of the zones. Equipment for flow measurement of settled sewage and return sludge is also installed. Every instrument is connected to a computer for data acquisition. To evaluate the efficiency of the aeration system the oxygenation transfer capacity has been calculated from the oxygen massbalance equation for each zone as a function of air flow. To solve this equation the respiration has to be known and this is done by a simple respirometer for samples of the MLSS in each zone. When the KLa-values are known as functions of the air flow the mass balance equation can be used to calculate the respiration rate in each zone. The computer has been logging data for 2 2 months, and it is possible to calculate the respiration rates in the different zones every hour during this period. It is very important to know the respiration along the tank and how it varies to get the optimal tapering of the diffusers when it is time to change the aeration system in the other 10 tanks. The calculations show a different pattern in the respiration over the year depending on the rate of nitrification. Another use of the calculation of the oxygenation transfer efficiency is to recognize if any long-term change occurs due to clogging of the diffusers.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Zarimah Mohd Hanafiah ◽  
Wan Hanna Melini Wan Mohtar ◽  
Hassimi Abu Hasan ◽  
Henriette Stokbro Jensen ◽  
Anita Klaus ◽  
...  

Abstract The fluctuation of domestic wastewater characteristic inhibits the current conventional microbial-based treatment. The bioremediation fungi has received attention and reported to be an effective alternative to treat industrial wastewater. Similar efficient performance is envisaged for domestic wastewater whereby assessed performance of fungi for varying carbon-to-nitrogen ratios in domestic wastewater is crucial. Thus, the performance of pre-grown wild-Serbian Ganoderma lucidum mycelial pellets (GLMPs) was evaluated on four different synthetic domestic wastewaters under different conditions of initial pH (pH 4, 5, and 7) and chemical oxygen demand (COD) to nitrogen (COD/N) ratio of 3.6:1, 7.1:1, 14.2:1, and 17.8:1 (C3.6N1, C7.1N1, C14.2N1, and C17.8N1). The COD/N ratios with a constant concentration of ammonia–nitrogen (NH3–N) were chosen on the basis of the urban domestic wastewater characteristics sampled at the inlet basin of a sewage treatment plant (STP). The parameters of pH, COD, and NH3–N were measured periodically during the experiment. The wild-Serbian GLMPs efficiently removed the pollutants from the synthetic sewage. The COD/N ratio of C17.8N1 wastewater had the best COD and NH3–N removal, as compared to the lower COD/N ratio, and the shortest treatment time was obtained in an acidic environment at pH 4. The highest percentage for COD and NH3–N removal achieved was 96.0% and 93.2%, respectively. The results proved that the mycelium of GLMP has high potential in treating domestic wastewater, particularly at high organic content as a naturally sustainable bioremediation system.


2015 ◽  
Vol 737 ◽  
pp. 635-638
Author(s):  
Ke Zhao ◽  
Zheng Kong

The general existing low temperature problem in winter of north-city sewage treatment plant in the process of waste water treatment seriously affect the efficiency of the work. The research objective of this paper is to conduct investigate in the application of AICS method in Jilin Province, a sewage treatment plant engineering example has been employed to monitor the effluent of the indicators of sewage treatment under the low temperature condition during the winter the winter low temperature under the condition of sewage treatment plant effluent of the indicators; to understand the operation situation of sewage treatment plant, and to study the efficiency of hydrolysis - AICS processing waste-water. The result shows that: hydrolysis - AICS process works well in low temperature condition, the per TN removal rate is 76.79%, average removal rate of NH3 - N is 85.76%, average TP removal rate was 93.4%, the average COD removal rate was 90.6%. The effluent meet the national level A discharge standard requirements of "urban sewage treatment plant pollutant discharge standard". And through the retrenchment of the second pond and other ancillary equipment, Hydrolysis-AICS process could efficiently reduce the costs. Besides, the advantages of Hydrolysis-AICS process also include occupying small area, and operating easily and simply. Therefore Hydrolysis-AICS process is very helpful to achieve the sewage discharging standard in the northern towns.


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