Reverse Micelle Liquid-Liquid Extraction of a Pharmaceutical Product

Reverse micelle extraction has received considerable attention in recent years due to its ability to selectively solubilise solutes from an aqueous phase, and in the case of biomolecules to maintain their biological activities. The apparent success of research on protein extraction from the aqueous phase using reverse micelle provides motivation to study the solubilisation of antibiotic. The objective of this study is to investigate the extraction of antibiotic (penicillin G is chosen as model antibiotic) from aqueous solution (forward extraction) and from the reverse micelle to a new aqueous solution (backward extraction). Sodium di(2-ethylhexyl)sulfosuccinate (AOT) is chosen as the surfactant and isooctane as the organic solvent. The UV-Vis spectrophotometer is used to determine the mass of penicillin G in solution after the extraction process. The extraction is expected to be influenced by the initial penicillin G concentration, the salt type and concentration in the aqueous phase, pH, and surfactant concentration. It is expected that as penicillin is an interfacially active compound that will interacts with AOT surfactant, the interfacial association will be dependent on both pH and surfactant concentration.

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Reverse Micelle Liquid-Liquid Extraction of Bovine Serum Albumin and Lysozyme

Jurnal Teknologi ◽

10.11113/jt.v58.1256 ◽

2012 ◽

pp. 07-11 ◽

Cited By ~ 2

Author(s):

Siti Hamidah Mohd Setapar ◽

Siti Norazimah Mohamad Aziz ◽

Constantine Joanne

Keyword(s):

Bovine Serum Albumin ◽

Serum Albumin ◽

Reverse Micelle ◽

Bovine Serum ◽

Surfactant Concentration ◽

Extraction Process ◽

Factors Affecting ◽

Forward Transfer ◽

Protein Bovine Serum Albumin ◽

Reverse Micelle Extraction

Reverse micelle extraction by using Sodium bis (2-ethylhexyl) Suffoccinate (AOT) of protein bovine serum albumin (BSA) and lysozyme was investigated in this research. Study of factors affecting the surfactant concentration and pH of aqueous for both forward and backward extraction process was performed in the research. The BSA concentrations were characterized by using the UV- spectrophotometer at wavelength, λ = 280 nm. The result indicated that the extraction percentage of lysozyme was higher than BSA in forward transfer for both parameters; however BSA demonstrated a better extraction performance in backward extraction process. The maximum lysozyme extracted in the forward extraction process was at 60 mM of surfactant concentration while for BSA was 100 mM since BSA is a bulky molecule and the size is larger than of lysozyme. Pengekstrakan misel terbalik dengan menggunakan Sodium bis (2-ethylhexyl) Suffoccinate (AOT) untuk protein bovine serum albumin (BSA) dan lysozyme disiasat dalam penyelidikan ini. Kajian tentang faktor-faktor yang mempengaruhi kepekatan surfaktan dan pH akueus untuk proses pengekstrakan hadapan dan ke belakang telah dijalankan. Kepekatan BSA telah dicirikan dengan menggunakan spektrofotometer UV pada panjang gelombang, λ = 280 nm. Hasil kajian telah menunjukkan bahawa peratusan pengekstrakan lysozyme adalah lebih tinggi daripada BSA bagi pemindahan ke hadapan untuk kedua-dua parameter, namun begitu BSA menunjukkan prestasi pengekstrakan yang lebih baik di dalam proses pengekstrakan kebelakang. Jumlah maksimum lysozyme yang diekstrak di dalam proses pengekstrakan ke hadapan adalah 60 mM kepekatan surfaktan manakala bagi BSA adalah 100 mM disebabkan BSA adalah molekul yang sangat besar dan ia mempunyai saiz yang lebih besar berbanding daripada lysozyme.

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Application of reverse micelle extraction process for amylase recovery using response surface methodology

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-007-0172-6 ◽

2007 ◽

Vol 31 (4) ◽

pp. 379-384 ◽

Cited By ~ 13

Author(s):

Manav B. Bera ◽

Parmjit S. Panesar ◽

Reeba Panesar ◽

Bahadur Singh

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Reverse Micelle ◽

Extraction Process ◽

Reverse Micelle Extraction

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Optimization of protein extraction process from jackfruit seed flour by reverse micelle system

Acta Scientiarum Technology ◽

10.4025/actascitechnol.v38i3.28111 ◽

2016 ◽

Vol 38 (3) ◽

pp. 283 ◽

Cited By ~ 1

Author(s):

Maycon Fagundes Teixeira Reis ◽

Renata Cristina Ferreira Bonomo ◽

Rita De Cássia Superbi de Sousa ◽

Cristiane Martins Veloso ◽

Rafael Da Costa Ilheu Fontan ◽

...

Keyword(s):

Reverse Micelle ◽

Protein Extraction ◽

Extraction Process ◽

Seed Flour ◽

Micelle System

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Improved mass spectrometric detection of acidic peptides by variations in the functional group pKa values of reverse micelle extraction agents

The Analyst ◽

10.1039/c7an02094e ◽

2018 ◽

Vol 143 (6) ◽

pp. 1434-1443 ◽

Cited By ~ 5

Author(s):

Bo Zhao ◽

Mahalia A. C. Serrano ◽

Meizhe Wang ◽

Tianying Liu ◽

Mallory R. Gordon ◽

...

Keyword(s):

Functional Groups ◽

Aqueous Phase ◽

Isoelectric Point ◽

Reverse Micelle ◽

Reverse Micelles ◽

Functional Group ◽

Selective Extraction ◽

Mass Spectrometric ◽

Acidic Peptides ◽

Reverse Micelle Extraction

Functional groups in reverse micelles maintain their aqueous phase pKa and allow selective extraction of peptides according to isoelectric point.

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Molecular Property-Tailored Soy Protein Extraction Process Using a Deep Eutectic Solvent

ACS Sustainable Chemistry & Engineering ◽

10.1021/acssuschemeng.1c01848 ◽

2021 ◽

Author(s):

Qingsong Chen ◽

Lingxiao Chaihu ◽

Xiaopeng Yao ◽

Xiwang Cao ◽

Wentao Bi ◽

...

Keyword(s):

Soy Protein ◽

Protein Extraction ◽

Deep Eutectic Solvent ◽

Extraction Process ◽

Molecular Property

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A comparative study on the extraction effects of common agents on collagen-based binders in mural paintings

Heritage Science ◽

10.1186/s40494-021-00519-y ◽

2021 ◽

Vol 9 (1) ◽

Author(s):

Jianghao Du ◽

Zhanyun Zhu ◽

Junchang Yang ◽

Jia Wang ◽

Xiaotong Jiang

Keyword(s):

Protein Structure ◽

Comparative Study ◽

Protein Extraction ◽

Guanidine Hydrochloride ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Extraction Process ◽

Ultraviolet Absorption Spectrum ◽

Mural Paintings ◽

The Impact

AbstractIn this paper, a comparative study was conducted on the extraction effects of six agents for collagen-based mural painting binders. These agents were used to extract the residual proteins in the non-aged and thermal aged samples. The protein extraction efficiencies of different extracting agents were quantitatively determined by bicinchoninic acid (BCA) method, and then processed by multivariate analysis of variance (MANOVA). The impact of the extraction process on the protein structure was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), ultraviolet absorption spectrum (UV) and circular dichroism (CD). The results showed that, for both non-aged and aged samples, the extraction efficiency of 2 M guanidine hydrochloride (GuHCl) was significantly higher than the other five agents, with less damage to the protein structure during the extraction process.

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Optimization of Microwave-Assisted Extraction of Phlorotannin From Sargassum swartzii (Turn.) C. Ag. With Ethanol/Water

Natural Product Communications ◽

10.1177/1934578x21996184 ◽

2021 ◽

Vol 16 (2) ◽

pp. 1934578X2199618

Author(s):

Tran Quoc Toan ◽

Tran Duy Phong ◽

Dam Duc Tien ◽

Nguyen Manh Linh ◽

Nguyen Thi Mai Anh ◽

...

Keyword(s):

Output Power ◽

Ethanol Concentration ◽

Biological Activities ◽

Solvent System ◽

Extraction Process ◽

Extraction Time ◽

Brown Macroalgae ◽

Experimental Conditions ◽

Total Extract ◽

Sargassum Swartzii

Sargassum is a genus of brown macroalgae in the class Phaeophyta, distributed widely in all oceans, including those of Vietnam. Species of this genus have been proven to possess diverse biological activities, such as antioxidant, anti-fungal, and anti-inflammatory, along with many benefits and applications for human health, including anti-diabetic, obesity, and thrombosis. These benefits arise from a diverse chemical composition, with compounds such as fucoidan, mannitol, and especially phlorotannin—a group of phenolic derivatives found predominantly in brown algae. In this study, we evaluated and optimized the factors that affected the extraction process of phlorotannins from Sargassum swartzii (Turn.) C. Ag., a common species of brown macroalgae in Vietnam. The process utilized ethanol and water as the solvent system, and the extraction process was assisted with the use of microwaves. To carry out optimization studies, Response Surface Methodology (RSM) was adopted according to a Central Composite Desisgn (CCD), taking four processing factors into consideration, ethanol concentration (%, v/v), extraction time (minutes), solvent/material ratio (v/w), and microwave output power (W) as independent variables. Phlorotannin concentration (mgPhE/g) and extract mass (mg) were regarded as optimization outcomes. Experimental conditions that produced the highest phlorotannin yield from 10 g of S. swartzii are as follows: Extraction time of 65 minutes, ethanol concentration of 52%, microwave output power of 613 W, and solvent/material ratio of 33/1 (v/w). These conditions corresponded to a phlorotannin concentration of 5.59 ± 0.11 mg PhE/g, and a total extract content of 27.88 ± 0.13 mg/g.

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