Effects of ion species in aqueous phase on protein extraction into reversed micellar solution

2003 ◽  
Vol 31 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Takumi Kinugasa ◽  
Aki Kondo ◽  
Emiko Mouri ◽  
Sakiko Ichikawa ◽  
Satomi Nakagawa ◽  
...  
Keyword(s):  
Aqueous Phase ◽  
Micellar Solution ◽  
Protein Extraction ◽  
Ion Species

scholarly journals Reverse Micelle Liquid-Liquid Extraction of a Pharmaceutical Product

2012 ◽  
Vol 545 ◽  
pp. 240-244 ◽  
Author(s):  
Siti Hamidah Mohd-Setapar ◽  
Siti Norazimah Mohamad-Aziz ◽  
N.H. Harun ◽  
S.H. Hussin
Keyword(s):  
Aqueous Solution ◽  
Aqueous Phase ◽  
Reverse Micelle ◽  
Surfactant Concentration ◽  
Protein Extraction ◽  
Biological Activities ◽  
Extraction Process ◽  
Pharmaceutical Product ◽  
Penicillin G ◽  
Reverse Micelle Extraction

Reverse micelle extraction has received considerable attention in recent years due to its ability to selectively solubilise solutes from an aqueous phase, and in the case of biomolecules to maintain their biological activities. The apparent success of research on protein extraction from the aqueous phase using reverse micelle provides motivation to study the solubilisation of antibiotic. The objective of this study is to investigate the extraction of antibiotic (penicillin G is chosen as model antibiotic) from aqueous solution (forward extraction) and from the reverse micelle to a new aqueous solution (backward extraction). Sodium di(2-ethylhexyl)sulfosuccinate (AOT) is chosen as the surfactant and isooctane as the organic solvent. The UV-Vis spectrophotometer is used to determine the mass of penicillin G in solution after the extraction process. The extraction is expected to be influenced by the initial penicillin G concentration, the salt type and concentration in the aqueous phase, pH, and surfactant concentration. It is expected that as penicillin is an interfacially active compound that will interacts with AOT surfactant, the interfacial association will be dependent on both pH and surfactant concentration.

Distribution and monomer activity of cholesterol in micellar bile salt: effect of cholesterol level

1987 ◽  
Vol 252 (3) ◽  
pp. G309-G314 ◽  
Author(s):  
K. Chijiiwa ◽  
W. G. Linscheer
Keyword(s):  
Partition Coefficient ◽  
Bile Salt ◽  
Cholesterol Level ◽  
Aqueous Phase ◽  
Micellar Solution ◽  
Salt Effect ◽  
Monomer Concentration ◽  
Micellar Solutions ◽  
Bile Salt Micelle ◽  
Everted Sacs

Despite the fact that uptake of cholesterol by the enterocyte occurs as a monomer from the intermicellar aqueous phase in equilibrium with micelle, the cholesterol monomer concentration in the aqueous phase and the partition coefficient between intermicellar aqueous phase and micellar aggregate have not been clarified. The present study deals with the distribution of cholesterol and monomer activity in constant bile salt-fatty acid micellar solutions with different cholesterol concentrations. In addition, uptake of cholesterol from these micellar solutions into rat jejunum was studied using everted sacs. Cholesterol monomer concentration in the aqueous phase increased linearly with the concentration of cholesterol in the micellar solution. Partition coefficient of cholesterol between the aqueous phase surrounding micelle and micellar aggregate was essentially constant at any cholesterol level (K = 3 X 10(-2)). Cholesterol monomer activities were linearly proportional to the cholesterol concentrations in the micellar solutions and correlated well with the rate of cholesterol uptake. It is concluded from these experiments that a partitioning phenomenon determines cholesterol monomer concentrations in the intermicellar aqueous phase from which the cholesterol is absorbed. After disappearance of the cholesterol monomers from the aqueous phase, these monomers are replaced by a shift of monomers from the intramicelle to intermicellar aqueous phase, under constant partition coefficient between extra- and intramicelle. The bile salt micelle provides a huge reservoir for partitioning of cholesterol monomers.

Verification of Protein Extraction Method by Magneto-Ferrite Treatment with EDS Analysis

2020 ◽  
Vol 140 (2) ◽  
pp. 128-129
Author(s):  
Suguru Kotani ◽  
Masaya Endo ◽  
Mahmudul Kabir ◽  
Kazutaka Mitobe
Keyword(s):  
Extraction Method ◽  
Protein Extraction ◽  
Eds Analysis

Aqueous Phase Diffusion in Crystalline Rock

1981 ◽  
Vol 11 ◽  
Author(s):  
M.H. Bradbury ◽  
D. Lever ◽  
D. Kinsey
Keyword(s):  
Radioactive Waste ◽  
Aqueous Phase ◽  
Water Movement ◽  
Degradation Products ◽  
Crystalline Rock ◽  
Crystalline Rocks ◽  
Radionuclide Transport ◽  
Fracture Networks ◽  
Phase Diffusion ◽  
Main Factors

One of the options being considered for the disposal of radioactive waste is deep burial in crystalline rocks such as granite. It is generally recognised that in such rocks groundwater flows mainly through the fracture networks so that these will be the “highways” for the return of radionuclides to the biosphere. The main factors retarding the radionuclide transport have been considered to be the slow water movement in the fissures over the long distances involved together with sorption both in man-made barriers surrounding the waste, and onto rock surfaces and degradation products in the fissures.

Modification of Low ĸ Materials for ULSI Multilevel Interconnects by Ion Implantation

2002 ◽  
Vol 716 ◽  
Author(s):  
Alok Nandini ◽  
U. Roy ◽  
A. Mallikarjunan ◽  
A. Kumar ◽  
J. Fortin ◽  
...  
Keyword(s):  
Dielectric Constant ◽  
Ion Implantation ◽  
Dielectric Materials ◽  
Dramatic Improvement ◽  
Force Microscopy ◽  
Low Dielectric ◽  
Quantitative Measurements ◽  
Hardness Increase ◽  
Xerogel Films ◽  
Ion Species

AbstractThin films of low dielectric constant (κ) materials such as Xerogel (ĸ=1.76) and SilkTM (ĸ=2.65) were implanted with argon, neon, nitrogen, carbon and helium with 2 x 1015 cm -2 and 1 x 1016 cm -2 dose at energies varying from 50 to 150 keV at room temperature. In this work we discuss the improvement of hardness as well as elasticity of low ĸ dielectric materials by ion implantation. Ultrasonic Force Microscopy (UFM) [6] and Nano indentation technique [5] have been used for qualitative and quantitative measurements respectively. The hardness increased with increasing ion energy and dose of implantation. For a given energy and dose, the hardness improvement varied with ion species. Dramatic improvement of hardness is seen for multi-dose implantation. Among all the implanted ion species (Helium, Carbon, Nitrogen, Neon and Argon), Argon implantation resulted in 5x hardness increase in Xerogel films, sacrificing only a slight increase (∼ 15%) in dielectric constant.

Liquid-liquid extraction of succinate using a dicopper cryptate

2020 ◽  
Author(s):  
Riccardo Mobili ◽  
Sonia La Cognata ◽  
Francesca Merlo ◽  
Andrea Speltini ◽  
Massimo Boiocchi ◽  
...  
Keyword(s):  
Aqueous Phase ◽  
Visible Spectrum ◽  
Tca Cycle ◽  
Residual Concentration ◽  
Waste Products ◽  
Liquid Liquid Extraction ◽  
The Tca Cycle ◽  
Quantitative Extraction ◽  
Uv Visible

<div> <p>The extraction of the succinate dianion from a neutral aqueous solution into dichloromethane is obtained using a lipophilic cage-like dicopper(II) complex as the extractant. The quantitative extraction exploits the high affinity of the succinate anion for the cavity of the azacryptate. The anion is effectively transferred from the aqueous phase, buffered at pH 7 with HEPES, into dichloromethane. A 1:1 extractant:anion adduct is obtained. Extraction can be easily monitored by following changes in the UV-visible spectrum of the dicopper complex in dichloromethane, and by measuring the residual concentration of succinate in the aqueous phase by HPLC−UV. Considering i) the relevance of polycarboxylates in biochemistry, as e.g. normal intermediates of the TCA cycle, ii) the relevance of dicarboxylates in the environmental field, as e.g. waste products of industrial processes, and iii) the recently discovered role of succinate and other dicarboxylates in pathophysiological processes including cancer, our results open new perspectives for research in all contexts where selective recognition, trapping and extraction of polycarboxylates is required. </p> </div>

A method for selective separation of scandium (III) from yttrium and a number of rare-earth elements for its subsequent determination

2018 ◽  
Vol 84 (11) ◽  
pp. 23-27
Author(s):  
M. I. Degtev ◽  
A. A. Yuminova ◽  
A. S. Maksimov ◽  
A. P. Medvedev
Keyword(s):  
Rare Earth ◽  
Aqueous Phase ◽  
Selective Isolation ◽  
Optimum Ratio ◽  
Extraction Condition ◽  
Sulfosalicylic Acid ◽  
Salting Out ◽  
Subsequent Determination ◽  
Toxic Organic Solvents ◽  
Metal Ions Extraction

The possibility of using an aqueous stratified system of antipyrine — sulfosalicylic acid — water for the selective isolation of scandium macro- and microquantities for subsequent determination is studied. The proposed extraction system eliminates the usage of toxic organic solvents. The organic phase with a volume of 1.2 to 2.0 ml, resulting from delamination of the aqueous phase containing antipyrine and sulfosalicylic acid is analysed to assess the possibility of using such systems for metal ions extraction. Condition necessary for the formation of such a phase were specified: the ratio of the initial components, their concentration, presence of inorganic salting out agents. The optimum ratio of antipyrine to sulfosalicylic acid is 2:1 at concentrations of 0.6 and 0.3 mol/liter in a volume of the aqueous phase of 10 ml. The obtained phase which consists of antipyrinium sulfosalicylate, free antipyrine and water, quantitatively extracts macro- and microquantities of scandium at pH = 1.54. Macro- and microquantities of yttrium, terbium, lanthanum, ytterbium and gadolinium are not extracted under the aforementioned conditions thus providing selective isolation of scandium from the bases containing yttrium, ytterbium, terbium, lanthanum, and gadolinium.

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