scholarly journals Optimization of Microwave-Assisted Extraction of Phlorotannin From Sargassum swartzii (Turn.) C. Ag. With Ethanol/Water

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199618
Author(s):  
Tran Quoc Toan ◽  
Tran Duy Phong ◽  
Dam Duc Tien ◽  
Nguyen Manh Linh ◽  
Nguyen Thi Mai Anh ◽  
...  
Keyword(s):  
Output Power ◽  
Ethanol Concentration ◽  
Biological Activities ◽  
Solvent System ◽  
Extraction Process ◽  
Extraction Time ◽  
Brown Macroalgae ◽  
Experimental Conditions ◽  
Total Extract ◽  
Sargassum Swartzii

Sargassum is a genus of brown macroalgae in the class Phaeophyta, distributed widely in all oceans, including those of Vietnam. Species of this genus have been proven to possess diverse biological activities, such as antioxidant, anti-fungal, and anti-inflammatory, along with many benefits and applications for human health, including anti-diabetic, obesity, and thrombosis. These benefits arise from a diverse chemical composition, with compounds such as fucoidan, mannitol, and especially phlorotannin—a group of phenolic derivatives found predominantly in brown algae. In this study, we evaluated and optimized the factors that affected the extraction process of phlorotannins from Sargassum swartzii (Turn.) C. Ag., a common species of brown macroalgae in Vietnam. The process utilized ethanol and water as the solvent system, and the extraction process was assisted with the use of microwaves. To carry out optimization studies, Response Surface Methodology (RSM) was adopted according to a Central Composite Desisgn (CCD), taking four processing factors into consideration, ethanol concentration (%, v/v), extraction time (minutes), solvent/material ratio (v/w), and microwave output power (W) as independent variables. Phlorotannin concentration (mgPhE/g) and extract mass (mg) were regarded as optimization outcomes. Experimental conditions that produced the highest phlorotannin yield from 10 g of S. swartzii are as follows: Extraction time of 65 minutes, ethanol concentration of 52%, microwave output power of 613 W, and solvent/material ratio of 33/1 (v/w). These conditions corresponded to a phlorotannin concentration of 5.59 ± 0.11 mg PhE/g, and a total extract content of 27.88 ± 0.13 mg/g.

scholarly journals Extraction Process of Polyphenols from Soybean (Glycine max L.) Sprouts: Optimization and Evaluation of Antioxidant Activity

Processes ◽  
2019 ◽  
Vol 7 (8) ◽  
pp. 489 ◽  
Author(s):  
Xuan Tien Le ◽  
Vo Luu Lan Vi ◽  
Tran Quoc Toan ◽  
Long Giang Bach ◽  
Tran Thanh Truc ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Dry Weight ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Polyphenol Content ◽  
Experimental Conditions ◽  
Total Polyphenol ◽  
Total Polyphenol Content

This research aimed to optimize the total polyphenol content (TPC) extracted from soybean sprout powder under different experimental parameters, including ethanol concentration (60–100% v/v), extraction temperature (40–80 °C), extraction time (15–150 min), material:solvent ratio (1:4–1:10 g/mL), the number extraction cycles (1, 2 and 3 times), the age of sprout (0–7 days), and the used part of the sprout (cotyledon, hypocotyl, or radicle). The obtained results were used in response surface methodology, in combination with a central composite design, to model the total polyphenol content (TPC) with respect to three variables, including ethanol concentration, extraction temperature, and material:solvent ratio. The experimental conditions for optimal recovery of TPC consisted of ethanol concentration of 88% (v/v), extraction temperature of 59 °C, material:solvent ratio of 1:6.5 g/mL, extraction time of 60 min, and 2 cycles of maceration. In addition, for maximal TPC, the sprout should undergo the germination of 5 days and the radicle fraction should be used. Based on the suggested optimum conditions, the obtained and verified TPC was 19.801 mg genistein (GE)/g dry weight (d.w.). The obtained dried extract also exhibited low antioxidant activity.

scholarly journals Optimization of Betalain Pigments Extraction Using Beetroot by-Products as a Valuable Source

Inventions ◽  
2021 ◽  
Vol 6 (3) ◽  
pp. 50
Author(s):  
Silvia Lazăr (Mistrianu) ◽  
Oana Emilia Constantin ◽  
Nicoleta Stănciuc ◽  
Iuliana Aprodu ◽  
Constantin Croitoru ◽  
...  
Keyword(s):  
Citric Acid ◽  
Acid Concentration ◽  
Ethanol Concentration ◽  
Extraction Process ◽  
Extraction Time ◽  
Quadratic Model ◽  
Citric Acid Concentration ◽  
Operating Variables ◽  
Conventional Solvent Extraction ◽  
Optimized Conditions

(1) Background: This study is designed to extract the bioactive compounds from beetroot peel for future use in the food industry. (2) Methods: Spectrophotometry techniques analyzed the effect of conventional solvent extraction on betalains and polyphenolic compounds from beetroot peels. Several treatments by varying for factors (ethanol and citric acid concentration, temperature, and time) were applied to the beetroot peel samples. A Central Composite Design (CCD) has been used to investigate the effect of the extraction parameters on the extraction steps and optimize the betalains and total polyphenols extraction from beetroot. A quadratic model was suggested for all the parameters analyzed and used. (3) Results: The maximum and minimum variables investigated in the experimental plan in the coded form are citric acid concentration (0.10–1.5%), ethanol concentration (10–50%), operating temperature (20–60 °C), and extraction time (15–50 min). The experimental design revealed variation in betalain content ranging from 0.29 to 1.44 mg/g DW, and the yield of polyphenolic varied from 1.64 to 2.74 mg/g DW. The optimized conditions for the maximum recovery of betalains and phenols were citric acid concentration 1.5%, ethanol concentration 50%, temperature 52.52 °C, and extraction time 49.9 min. (4) Conclusions: Overall, it can be noted that the extraction process can be improved by adjusting operating variables in order to maximize the model responses.

scholarly journals Optimization of Total Phenolic and Flavonoid Contents of Defatted Pitaya (Hylocereus polyrhizus) Seed Extract and Its Antioxidant Properties

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 787 ◽  
Author(s):  
Siti Atikah Zulkifli ◽  
Siti Salwa Abd Gani ◽  
Uswatun Hasanah Zaidan ◽  
Mohd Izuan Effendi Halmi
Keyword(s):  
High Performance ◽  
Ethanol Concentration ◽  
Antioxidant Properties ◽  
Extraction Process ◽  
Seed Extract ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Antioxidant Power ◽  
Flight Mass Spectrometry

The present study was conducted to optimize extraction process for defatted pitaya seed extract (DPSE) adopting response surface methodology (RSM). A five-level central composite design was used to optimize total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2′-azino-bis (3-ethylbenzothizoline-6-sulfonic acid (ABTS) activities. The independent variables included extraction time (30–60 min), extraction temperature (40–80 °C) and ethanol concentration (60%–80%). Results showed that the quadratic polynomial equations for all models were significant at (p < 0.05), with non-significant lack of fit at p > 0.05 and R2 of more than 0.90. The optimized extraction parameters were established as follows: extraction time of 45 min, extraction temperature of 70 °C and ethanol concentration of 80%. Under these conditions, the recovery of TPC, TFC, and antioxidant activity based on FRAP and ABTS were 128.58 ± 1.61 mg gallic acid equivalent (GAE)/g sample, 9.805 ± 0.69 mg quercetin equivalent (QE)/g sample, 1.23 ± 0.03 mM Fe2+/g sample, and 91.62% ± 0.15, respectively. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) analysis identified seven chemical compounds with flavonoids constituting major composition of the DPSE.

scholarly journals Influence of extraction conditions on the recovery lipids extracted from the dry biomass of duckweed Lemna minor

2018 ◽  
Vol 245 ◽  
pp. 18004 ◽  
Author(s):  
Yulia Smyatskaya ◽  
Natalia Politaeva ◽  
Amira Toumi ◽  
Lubov Olshanskaya
Keyword(s):  
Polar Solvent ◽  
Lemna Minor ◽  
Lipid Fraction ◽  
Volume Ratio ◽  
Solvent System ◽  
Extraction Process ◽  
Dry Mass ◽  
Extraction Time ◽  
Total Lipids ◽  
Dry Biomass

Previous studies on the composition of lipid content of higher aquatic plants can be found in the literature. The present work aims to study the effect of various solvent extraction systems on the recovery of lipids extracted from the dry biomass of Lemna minor, using the same polar solvent (ethanol) and variating the non-polar solvent as follow: methanol-ethanol, chloroform-ethanol, hexane-ethanol, diethyl ether-ethanol, petroleum ether-ethanol at different volume ratio. The goal is to select a Sohxlet extraction method that shortens the extraction duration and increases the lipid recovery using various solvents. To intensify the extraction process, homogenization was used as a cell disruption method. It is shown that the yield of total lipids is increased by using homogenization from 4.6% to 6.0%. When studying the influence of the solvent system, the highest yield of total lipids was obtained by using hexane-ethanol with a volume ration of 1:1 (8.0% total lipids by dry mass). The yield of lipids recovered from dry biomass of Lemna minor increases by increasing the extraction time. The optimum extraction time was found to be no more than 300 minutes. The composition of the lipid fraction is mainly represented by sterols, fatty acids and triglycerides.

Optimization of Murrayafoline A ethanol extraction process from the roots of Glycosmis stenocarpa, and evaluation of its Tumorigenesis inhibition activity on Hep-G2 cells

2021 ◽  
Vol 19 (1) ◽  
pp. 830-842
Author(s):  
Quoc Toan Tran ◽  
The Dan Pham ◽  
Thanh Duong Nguyen ◽  
Van Huyen Luu ◽  
Huu Nghi Do ◽  
...  
Keyword(s):  
Large Scale ◽  
Extraction Efficiency ◽  
Raw Materials ◽  
Biological Activities ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Hep G2 ◽  
Independent Parameters

Abstract Glycosmis stenocarpa is a species of shrub found in the Northern provinces of Vietnam. Its roots contain different carbazolic derivatives, mainly Murrayafoline A (Mu-A), which exhibits valuable biological activities. In this study, we performed an extraction of Mu-A from the roots of G. stenocarpa and optimized this process using response surface methodology (RSM) according to a central composite design, with three independent parameters including extraction time (min), extraction temperature (°C), and solvent/material ratio (mL/g). Two dependent variables were the Mu-A content (mg/g raw materials) and extraction efficiency (%). The optimal conditions to extract Mu-A were found to be as follows: extraction temperature, 67°C; extraction time, 165 min; and solvent/material ratio, 5:1. Under these conditions, the Mu-A content and extraction efficiency were 38.94 ± 1.31 mg/g raw materials and 34.98 ± 1.18%, respectively. Mu-A exhibited antiproliferation and antitumor-promoting activity against the HepG-2 cell line. The present optimization work of Mu-A extraction from G. stenocarpa roots contributed to the attempt of designing a large-scale extraction process for the compound and further exploitation of its potential in vivo applications.

scholarly journals First Report on Tertiary Amine as a Co2 Switchable Solvent for Hypersaline Trebouxiophycean Microalgae Towards Greener and Competent Lipid Extraction

2020 ◽  
Author(s):  
Susaimanickam Anto ◽  
M Premalatha ◽  
Thangavel Mathimani
Keyword(s):  
Fatty Acid ◽  
Energy Efficient ◽  
Lipid Extraction ◽  
Solvent System ◽  
Extraction Process ◽  
Extraction Time ◽  
Tertiary Amines ◽  
Microalgal Biomass ◽  
Lipid Yield ◽  
Fame Profile

Abstract Considering the momentous cost drivers in energy efficient algal biorefinery processes, a green alternative in the lipid extraction process from microalgae is anticipated. Switchable solvent system using tertiary amines namely DMBA (Dimethylbenzylamine), DMCHA (Dimethylcyclohexylamine), and DIPEA (Diisopropylethylamine) for lipid extraction from wet hypersaline microalgae was investigated in this study. Interestingly, showed that at 1:1 (v/v of fresh DMBA solvent: microalgal biomass), and for 1 h extraction time, the lipid yield was 41.9, 26.6, and 33.3% for Chlorella sp. NITT 05, Chlorella sp. NITT 02, and Picochlorum sp. NITT 04 respectively and for recovered DMBA solvent at 1:1 (v/v) and for 1 hour extraction time, the lipid yield was 40.8, 25.97, and 32%, respectively. Similarly, lipid extraction using DMCHA solvent for Chlorella sp. NITT 05, Chlorella sp. NITT 02, and Picochlorum sp. NITT 04 at 1:1 (v/v of solvent: microalgal biomass) and 1 h extraction time showed 34.28, 24.24 and 23.33% lipids, respectively for fresh solvent and 34.01, 24.24 and 23.18% for recovered solvent respectively; while DIPEA was not competent in lipid extraction from three tested microalgae. FAME profile shows the presence of major saturated fatty acid as C16:0 (~ 30%) and major unsaturated fatty acid as C18:1 (~17%).

scholarly journals Optimization of canthaxanthin extraction from fermented biomass of Paracoccus carotinifacuens VTP20181 bacteria strain isolated in Vietnam

2021 ◽  
Vol 9 (1) ◽  
pp. 117-125
Author(s):  
Le Xuan Duy ◽  
Tran Quoc Toan ◽  
Dang Viet Anh ◽  
Nguyen Phi Hung ◽  
Trinh Thi Thu Huong ◽  
...  
Keyword(s):  
Output Power ◽  
Theoretical Calculations ◽  
Extraction Process ◽  
Ultrasonic Waves ◽  
Extraction Time ◽  
Carotenoid Content ◽  
Extraction Temperature ◽  
Technological Parameters ◽  
Pharmaceutical Industries ◽  
Biomass Extract

Introduction. The bacterium strain Paracoccus carotinifaciens VTP20181 isolated in Vietnam produces canthaxanthin, a carotenoid widely used in the food and pharmaceutical industries. The aim of this work was to determine optimal parameters for canthaxanthin extraction from fermented biomass of P. carotinifaciens VTP20181. Study objects and methods. First, a series of single factor investigations were carried out in regard to maximal carotenoid content in the biomass extract obtained by using ultrasonic waves. Four parameters of the extraction process, such as extraction temperature, solvent/material ratio, extraction time, and ultrasonic output power, were studied. The obtained results were then optimized by using Response Surface Methodology (RSM) and Box-Behnken experimental design. Results and discussion. The optimal technological parameters of the extraction process included extraction temperature of 35°C, solvent/material ratio of 9.5:1 (v/w), extraction time of 90 min, and ultrasonic output power of 145 W. Under optimal conditions, canthaxanthin and total carotenoid contents were determined as 14.95 ± 0.12 and 18.21 ± 0.11 mg/g respectively, which were compatible with theoretical calculations ‒ 15.074 and 18.263 mg/g, respectively. Conclusion. Current results confirmed that the strain of halophilic P. carotinifaciens VTP20181 is a potential source for canthaxanthin biosynthesis.

Extraction Process Optimization of Endo-Polysaccharides from Phellinus igniarius

2014 ◽  
Vol 881-883 ◽  
pp. 347-350
Author(s):  
Yan Xu ◽  
Hui Cao ◽  
Shuang Shuang Gu ◽  
Sha Sha Wang ◽  
Jun Wang ◽  
...  
Keyword(s):  
Trichloroacetic Acid ◽  
Ethanol Concentration ◽  
Removal Rate ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Phellinus Igniarius ◽  
Maximal Yield ◽  
Final Ethanol Concentration

Polysaccharides ofPhellinus igniariushave remarkable bioactivities and attracted considerable attention. In this study, extraction process of endo-polysaccharides fromP. igniariuwas investigated. The extraction conditions including ratio of sporocarp to water, extraction time, extraction temperature, extraction times were optimized. The experimental results indicated that the maximal yield of endo-polysaccharides fromP. igniariuwas 2.93 %, and the optimal conditions were as follows: ratio of sporocarp to water 1:30, extraction time 2 h, extraction temperature 90 °C, extraction twice. The best final ethanol concentration was 80 %. In addition, protein removal rate could reach 90.6 % using trichloroacetic acid method in extracting polysaccharides process.

scholarly journals Optimization Of Microwave-Assisted Extraction Of Flavonoids From Binahong (Anredera cordifolia) Leaves Using Respon Surface Methodology

2020 ◽  
Vol 7 (3) ◽  
pp. 10-19
Author(s):  
Zaldy Rusli ◽  
Bina Lohita Sari ◽  
Novi Fajar Utami ◽  
S Sabila
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Antioxidant Activities ◽  
Extraction Process ◽  
Extraction Time ◽  
Microwave Assisted Extraction ◽  
Optimum Conditions ◽  
Microwave Assisted ◽  
Assisted Extraction

Binahong (Anredera scandens (L.) Moq.) leaves are one of the plants that empirically can be used for wound healing and contain flavonoids which have antibacterial and antioxidant activities. Determination of the optimum conditions of the Binahong leaf extraction process needs to be done to ensure the extraction quality of the Binahong leaves associated with the given activity. Microwave Assisted Extraction (MAE) is an extraction that utilizes microwave radiation to heat the solvent quickly and efficiently. This study aims to determine the most effective binahong leaf extraction conditions that produce optimal levels of flavonoids and antioxidant activity. The extraction process was carried out using the Microwave assisted extraction (MAE) method. Determination of optimum conditions is done based on Response Surface Method (RSM) with variable ethanol concentration (70%, 80% and 90%), extraction time (4, 12 and 20 minutes) and power (450, 600, and 800 watts) using Box- Behnken Design (BBD) with Design Expert 7.0 software. The extract quality parameters measured were total flavonoid levels and antioxidant activity. The results showed the optimum conditions with the BBD method were obtained at an ethanol concentration of 81.49%, extraction time of 13.84 minutes, and power of 626.19 watts with flavonoid levels of 3.8561% and antioxidant activity (IC50) of 95.51834 ppm with active categories.

scholarly journals PHENOLIC COMPOSITION AND ANTIOXIDANT ACTIVITY OF GREEN-SOLVENTS-BASED EXTRACTS OF RED ONION WASTES

AGROFOR ◽  
2019 ◽  
Vol 3 (3) ◽  
Author(s):  
Simona OANCEA ◽  
Maria RADU
Keyword(s):  
Antioxidant Activity ◽  
Solvent System ◽  
Extraction Process ◽  
Ethanol Solution ◽  
Extraction Time ◽  
Antioxidant Compounds ◽  
Green Solvents ◽  
Valuable Compounds ◽  
Two Parameters ◽  
Sample Ratio

Onion represents one of the most important crop, based on its production,consumption and high pharmacological potential supported by its chemicalcomposition. Phytochemicals of phenolic structure, in particular anthocyanins, areamong the strongest antioxidant compounds with large biological and industrialapplications. Because their conventional extraction involves the use of highamounts of polar organic solvents, there is a strong requirement for development ofnew strategies based on greener solvents. The aim of the present paper was toextract valuable compounds of polyphenolic structure from red onion wastes usingdeep eutectic solvents, as green strategy for their isolation. In addition, the totalantioxidant activity as measured by ferric reducing antioxidant capacity assay wasinvestigated. Extraction performed at 40oC in the selected solvent system wasoptimized for two parameters, solvent/sample ratio and extraction time. Theobtained results showed the highest content of anthocyanins and antioxidantactivity at a solvent/sample ratio of 30/1 and the extraction time of 90 min, at 40oC.Regarding the total phenolics and flavonoids, the optimal extraction parameterswere 20/1 solvent/sample ratio and 90 min, at 40oC. Compared to conventionalextraction in organic solvent, the values of the antioxidant activity were similar,while those of the content of bioactive compounds were higher in extracts obtainedin ethanol solution. By further optimization of the extraction process, the obtainedproducts may find useful application in obtaining natural ingredients intended fordifferent purposes.

Sign in / Sign up

Export Citation Format

Share Document