Inhibitory Effect on Lipid and Protein Oxidation of Clove Extracts in Surimi from Silver Carp (Hypophthalmichthys molitrix) and the Possible Mode of Action

2012 ◽  
Vol 554-556 ◽  
pp. 1447-1454
Author(s):  
Jian Chun Han ◽  
Qian Liu ◽  
Yong Gen Zhang ◽  
Shuang Mei Li ◽  
Qian Chen ◽  
...  
Keyword(s):  
Protein Oxidation ◽  
Silver Carp ◽  
Thiobarbituric Acid ◽  
Natural Antioxidants ◽  
Inhibitory Effect ◽  
Thiobarbituric Acid Reactive Substance ◽  
Dependent Manner ◽  
Hypophthalmichthys Molitrix ◽  
C Storage ◽  
Lipid And Protein Oxidation

The experiment was conducted to assess the antioxidant efficacy of clove extract (CE) in surimi from silver carp during -1°C storage. CE was added to surimi at 0.5%, 1.0%, and 1.5% (w/w) further determined its antioxidant efficacy. Compared with control (without antioxidants), CE treatments significantly decreased the thiobarbituric acid reactive substance (TBARS values), a*-value, and total carbonyls content, reduced the total sulfhydryl loss of surimi in a dose-dependent manner (P < 0.05), and both ATPase activity increased with the increasing of the storage time (P < 0.05).The results suggested the positive effect of CE to retard lipid and protein oxidation and discoloration of surimi, and its potential use as natural antioxidants in fish products.

The application of natural antioxidants via brine injection protects Iberian cooked hams against lipid and protein oxidation

Meat Science ◽  
2016 ◽  
Vol 116 ◽  
pp. 253-259 ◽  
Author(s):  
Mónica Armenteros ◽  
David Morcuende ◽  
Jesús Ventanas ◽  
Mario Estévez
Keyword(s):  
Protein Oxidation ◽  
Natural Antioxidants ◽  
Lipid And Protein Oxidation ◽  
Brine Injection

Antimicrobial and Antioxidant Activities of Deer Blood Hydrolysate on Fresh Beef during Refrigerated Storage

2013 ◽  
Vol 634-638 ◽  
pp. 1417-1422
Author(s):  
Shuai Wang ◽  
Feng Bo Xu ◽  
Li Fu Zhu ◽  
Yan Ze Yu ◽  
Chun Zi Na
Keyword(s):  
Lipid Oxidation ◽  
Ph Value ◽  
Antioxidant Activities ◽  
Ground Beef ◽  
Thiobarbituric Acid ◽  
Natural Antioxidants ◽  
Inhibitory Effect ◽  
Plate Count ◽  
Refrigerated Storage ◽  
Color Deterioration

The aim of this work was to determine the inhibitory effect of deer blood hydrolysates addition on lipid oxidation, color deterioration and microbial contamination of ground beef during during 9 days of refrigerated storage. The pH value, total plate count, thiobarbituric acid-reactive substances value (TBARS), total volatile basic nitrogen (TVB-N) and color were also determined. The results showed that the treatments added hydrolysates had significant inhibitory effects on lipid oxidation and kept the red color of ground beef in 9 days, when compared with controls (P < 0.05). Furthermore, the inhibitory effect enhanced as the adding level of the hydrolysates increased (P < 0.05). These results suggest that deer blood hydrolysates are good source of natural antioxidants.

Effect of Superchilling Storage on Quality Characterizes and Physical Properties of Surimi from Silver Carp (Hypophthalmichthys molitrix) as Compared with Cooling Freshness Preservation

2012 ◽  
Vol 554-556 ◽  
pp. 978-984
Author(s):  
Qian Liu ◽  
Jian Chun Han ◽  
Yong Gen Zhang ◽  
Shuang Mei Li ◽  
Jing Li ◽  
...  
Keyword(s):  
Atpase Activity ◽  
Physical Properties ◽  
Storage Time ◽  
Ph Value ◽  
Silver Carp ◽  
Thiobarbituric Acid ◽  
Raw Material ◽  
Hypophthalmichthys Molitrix ◽  
Thiobarbituric Acid Reactive Substances ◽  
Fresh Products

Quality characterizes of surimi of silver carp at two different conditions of superchilling and cooling freshness preservation. The pH value, thiobarbituric acid-reactive substances (TBARS value), protein soubility and ATPase activity were studied. The result showed that pH value and TBARS value increased with the increasing of the storage time (P < 0.05), protein soubility and both ATPase activity decreased with the increasing of the storage time (P < 0.05). In general, superchilling is a good way to preserve freshness of fresh products and the raw material before processing, and also could have great effect on improving the quality characterizes of surimi and prolong its shelf life.

Degradation of oxidative stress-induced denatured albumin in rat liver endothelial cells

2005 ◽  
Vol 289 (3) ◽  
pp. C531-C542 ◽  
Author(s):  
Ryuji Bito ◽  
Sayaka Hino ◽  
Atsushi Baba ◽  
Miharu Tanaka ◽  
Haruka Watabe ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Rat Liver ◽  
Laser Scanning ◽  
Thiobarbituric Acid ◽  
Serum Levels ◽  
Inhibitory Effect ◽  
Endocytic Pathway ◽  
Thiobarbituric Acid Reactive Substance ◽  
Liver Endothelial Cells

We previously identified conformationally denatured albumin (D2 and D3 albumin) in rats with endotoxicosis (Bito R, Shikano T, and Kawabata H. Biochim Biophys Acta 1646: 100–111, 2003). In the present study, we attempted first to confirm whether the denatured albumins generally increase in conditions of oxidative stress and second to characterize the degradative process of the denatured albumin using primary cultured rat liver endothelial cells. We used five models of oxidative stress, including endotoxicosis, ischemic heart disease, diabetes, acute inflammation, and aging, and found that serum concentrations of D3 albumin correlate with the serum levels of thiobarbituric acid-reactive substance ( R = 0.87), whereas the concentrations of D2 albumin are 0.52. Ligand blot analysis showed that the D3 albumin binds to gp18 and gp30, which are known endothelial scavenger receptors for chemically denatured albumin. Primary cultured rat liver endothelial cells degraded the FITC-D3 albumin, and the degradation rate decreased to ∼60% of control levels in response to anti-gp18 and anti-gp30 antibodies, respectively. An equimolar mixture of these antibodies produced an additive inhibitory effect on both uptake and degradation, resulting in levels ∼20% those of the control. Furthermore, filipin and digitonin, inhibitors of the caveolae-related endocytic pathway, reduced the FITC-D3 albumin uptake and degradation to <20%. Laser-scanning confocal microscopic observation supported these data regarding the uptake and degradation of D3 albumin. These results indicate that conformationally denatured D3 albumin occurs generally under oxidative stress and is degraded primarily via gp18- and gp30-mediated and caveolae-related endocytosis in liver endothelial cells.

Antioxidant Activity of Seabuckthorn (Hippophae rhamnoides) Leaves Extracts on Pork Patties during Refrigerated Storage

2013 ◽  
Vol 781-784 ◽  
pp. 1500-1507
Author(s):  
Zhong Jin ◽  
Qian Liu ◽  
Bao Hua Kong ◽  
Jing Li ◽  
Qian Chen
Keyword(s):  
Ph Value ◽  
Antioxidant Activities ◽  
Thiobarbituric Acid ◽  
Hippophae Rhamnoides ◽  
Protein Carbonyls ◽  
Dependent Manner ◽  
Hippophaë Rhamnoides ◽  
Lipid And Protein Oxidation ◽  
Pork Patties ◽  
Dose Dependent

This experiment was conducted to assess the antioxidant efficacy of Seabuckthorn (Hippophae rhamnoides) leaves extracts (SLE) in raw pork patties during chilled storage at 4 °C for 0-7 days. The pork patties were analyzed periodically for chemical characteristics, such as pH value, colour, thiobarbituric acid-reactive substances value (TBARS), peroxide value (POV) and protein carbonyls content. Compared with the control, SLE treatments significantly decreased the TBARs and POV values and carbonyls formation of pork patties in a dose-dependent manner (P < 0.05), which showed that the SLE significantly inhibited lipid and protein oxidation. The results of this study suggested that the antioxidant activities of seabuckthorn leaves extracts could have great effect on improving the quality characterizes of pork patties and prolong its shelf life.

scholarly journals Aromatic indolinic aminoxyls as antioxidants in cardiac sarcoplasmic reticulum lipid and protein oxidation.

2002 ◽  
Vol 49 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Dorota Kulawiak-Gałaska ◽  
Michał Woźniak ◽  
Lucedio Greci
Keyword(s):  
Lipid Peroxidation ◽  
Sarcoplasmic Reticulum ◽  
Protein Oxidation ◽  
Acyl Chain ◽  
Thiobarbituric Acid ◽  
Membrane System ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Lipid And Protein Oxidation ◽  
Lipid Peroxidation Inhibition ◽  
Membrane Components

The results presented demonstrate the influence of aromatic indolinic aminoxyls: 1,2-dihydro-2-ethyl-2-phenyl-3H-indole-3-phenylimino-1-oxyl (IA-C2) and 1,2-dihydro-2-octadecyl-2-phenyl-3H-indole-3-phenylimino-1-oxyl (IA-C18) on oxidation of lipids and proteins of cardiac sarcoplasmic reticulum membranes. We have used doxorubicin and t-butyl hydroperoxide as agents inducing oxidative stress in isolated rat cardiac sarcoplasmic reticulum membrane system. Carbonyl groups were measured as the end product of membrane protein oxidation, and thiobarbituric acid reactive substances were assessed as a marker of lipid peroxidation. Inhibition of peroxidation of certain membrane components depends on the length of acyl chain. Aminoxyl IA-C2 inhibits the lipid peroxidation process while IA-C18 is an efficient protector against protein oxidation.

scholarly journals l-carnosine (β-alanyl-l-histidine) and carcinine (β-alanylhistamine) act as natural antioxidants with hydroxyl-radical-scavenging and lipid-peroxidase activities

1994 ◽  
Vol 304 (2) ◽  
pp. 509-516 ◽  
Author(s):  
M A Babizhayev ◽  
M C Seguin ◽  
J Gueyne ◽  
R P Evstigneeva ◽  
E A Ageyeva ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Direct Reduction ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Natural Antioxidants ◽  
Peroxyl Radicals ◽  
Lipid Phase ◽  
Oh Radicals ◽  
Thiobarbituric Acid Reactive Substance ◽  
Lipid Hydroperoxides

Carnosine (beta-alanyl-L-histidine) and carcinine (beta-alanylhistamine) are natural imidazole-containing compounds found in the non-protein fraction of mammalian tissues. Carcinine was synthesized by an original procedure and characterized. Both carnosine and carcinine (10-25 mM) are capable of inhibiting the catalysis of linoleic acid and phosphatidylcholine liposomal peroxidation (LPO) by the O2(-.)-dependent iron-ascorbate and lipid-peroxyl-radical-generating linoleic acid 13-monohydroperoxide (LOOH)-activated haemoglobin systems, as measured by thiobarbituric-acid-reactive substance. Carcinine and carnosine are good scavengers of OH. radicals, as detected by iron-dependent radical damage to the sugar deoxyribose. This suggests that carnosine and carcinine are able to scavenge free radicals or donate hydrogen ions. The iodometric, conjugated diene and t.l.c. assessments of lipid hydroperoxides (13-monohydroperoxide linoleic acid and phosphatidylcholine hydroperoxide) showed their efficient reduction and deactivation by carnosine and carcinine (10-25 mM) in the liberated and bound-to-artificial-bilayer states. This suggests that the peroxidase activity exceeded that susceptible to direct reduction with glutathione peroxidase. Imidazole, solutions of beta-alanine, or their mixtures with peptide moieties did not show antioxidant potential. Free L-histidine and especially histamine stimulated iron (II) salt-dependent LPO. Due to the combination of weak metal chelating (abolished by EDTA), OH. and lipid peroxyl radicals scavenging, reducing activities to liberated fatty acid and phospholipid hydroperoxides, carnosine and carcinine appear to be physiological antioxidants able to efficiently protect the lipid phase of biological membranes and aqueous environments.

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