Purification and Biochemical Characterization of Trypsin Inhibitor from Oyster

2012 ◽  
Vol 577 ◽  
pp. 119-124
Author(s):  
Yuan Hui Zhao ◽  
Ming Yong Zeng ◽  
Xia Li
Keyword(s):  
Liquid Chromatography ◽  
Trypsin Inhibitor ◽  
High Performance ◽  
Biochemical Characterization ◽  
Gel Filtration ◽  
Reversed Phase ◽  
Phase Liquid ◽  
Lung Adenocarcinoma A549 ◽  
Head Inhibitor

In this paper, the purification and biochemical characterization of the endogenous oyster (Crassostrea gigas) trypsin inhibitor were researched. A oyster trypsin inhibitor（OTI）has been purified by successive ammonium sulfate precipitation, gel filtration, affinity chromatography and high performance reversed-phase liquid chromatography. OTI has a molecular weight of approximately 5036 Da estimated by high performance size exclusive liquid chromatography. OTI was heat-, acid- and basic-stable competitive trypsin inhibitor. And OTI was double-head inhibitor with the inhibition constant (Ki) value of 1.644×10-2 mmol L-1. OTI was composed of nine kinds of amino acid, and rich in cysteine, alanine and glutamic acid. Furthermore, OTI can inhibit the proliferations of human lung adenocarcinoma A549 cell and human cervical cancer Hela cell

scholarly journals Correlation between retention and C-18 silica gel surface coverage in reversed-phase liquid chromatography

2000 ◽  
Vol 65 (11) ◽  
pp. 811-818
Author(s):  
Marijana Acanski ◽  
Slobodan Petrovic ◽  
Vjera Pejanovic ◽  
Julijana Petrovic
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Carbon Content ◽  
Silica Gel ◽  
High Performance ◽  
Surface Coverage ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Retention Behaviour ◽  
Phase Liquid

The effect of C-18 silica gel surface coverage on the retention behaviour of some estrone derivatives in reversed phase high performance liquid chromatography has been studied. Two commercial columns with different C-18 coverage, Spherisorb ODS-1(8 % carbon content) and Li Chrosorb RP-18(22% carbon content), using methanol-water as the eluent, were used.

The use of reversed-phase high-performance liquid chromatography to detect proteolytic activity from human pancreas in a radioimmunoassay for corticotrophin-releasing factor

1987 ◽  
Vol 114 (1) ◽  
pp. 147-151 ◽  
Author(s):  
D. S. Jessop ◽  
R. L. Patience ◽  
D. Cunnah ◽  
L. H. Rees
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Proteolytic Activity ◽  
High Performance ◽  
Gel Filtration ◽  
Reversed Phase ◽  
Pancreatic Tissue ◽  
Acid Extraction ◽  
Human Pancreas ◽  
Corticotrophin Releasing Factor

ABSTRACT Degradation of tracer during a radioimmunoassay (RIA) can result in false-positive concentrations of immunoreactivity being reported in a biological sample. A technique has been developed using reversed-phase high-performance liquid chromatography (HPLC) to detect proteolytic degradation of corticotrophin-releasing factor-41 (CRF-41) during incubation with tissue extracts under RIA conditions. Human pancreatic tissue was extracted in HCl or urea and incubated with 125I-labelled CRF-41 at neutral pH for 18 h. When samples were analysed by HPLC and fractions counted for radioactivity, tracer was extensively degraded. Heating extracts at 85 °C or adding lima bean trypsin inhibitor to the medium prevented degradation. Pancreatic tissue extracted in HCl was analysed by gel filtration and HPLC, and fractions were subjected to RIA for CRF-41. A peak of immunoreactivity was detected by both chromatographic methods. However, when this material was incubated with tracer and analysed by HPLC, the tracer was degraded, indicating that proteolytic activity remained after acid extraction and two forms of chromatography. J. Endocr. (1987) 114, 147–151

Separation and characterization of oligomers by reversed-phase high-performance liquid chromatography; a study on well-defined oligothiophenes

2001 ◽  
Vol 911 (1) ◽  
pp. 13-26 ◽  
Author(s):  
E.C Vonk ◽  
B.M.W Langeveld-Voss ◽  
J.L.J van Dongen ◽  
R.A.J Janssen ◽  
H.A Claessens ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reversed Phase
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