The Effect of Glucose on Saccharomyces cerevisiae Cell Biosorption of Strontium under Simulation Culture Conditions

2012 ◽  
Vol 610-613 ◽  
pp. 173-176
Author(s):  
Ming Xue Liu ◽  
Fa Qin Dong ◽  
Dong Zhang ◽  
Wu Kang ◽  
Hong Fu Wei ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Physical Adsorption ◽  
Growth Cycle ◽  
Culture Conditions ◽  
Strontium Concentration ◽  
Biosorption Process ◽  
Strontium Ions ◽  
Effect Of Glucose

The effect of glucose on Saccharomyces cerevisiae cell biosorption of strontium under simulation culture conditions was studied in this research. The results showed that the glucose adding did not abviously increase the biosorption efficiency in 30-150 min and after 8 h. The glucose adding might stimulate the S.cerevisiae cell biosorption of strontium ions in 4-8 h for high initial strontium concentration. The results indicated that the glucose adding only stimulated S.cerevisiae cell biosorption of strontium at certain biosorption time and the biosorption process first was a physical adsorption followed bioaccumulation according to interface process and cell growth cycle under simulation culture conditions.

scholarly journals Contribution of surface functional groups and interface interaction to biosorption of strontium ions by Saccharomyces cerevisiae under culture conditions

RSC Advances ◽  
2017 ◽  
Vol 7 (80) ◽  
pp. 50880-50888 ◽  
Author(s):  
Mingxue Liu ◽  
Faqin Dong ◽  
Wei Zhang ◽  
Xiaoqin Nie ◽  
Hongfu Wei ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Functional Groups ◽  
Functional Group ◽  
Culture Conditions ◽  
Surface Functional Group ◽  
Group Contributions ◽  
Surface Functional Groups ◽  
Interface Interaction ◽  
Strontium Ions

Surface functional group contributions to biosorption of strontium ions bySaccharomyces cerevisiaeas well as interface interactions were elucidated.

scholarly journals The rye Mutants Identify a Role for Ssn/Srb Proteins of the RNA Polymerase II Holoenzyme During Stationary Phase Entry in Saccharomyces cerevisiae

Genetics ◽  
2001 ◽  
Vol 157 (1) ◽  
pp. 17-26 ◽  
Author(s):  
Ya-Wen Chang ◽  
Susie C Howard ◽  
Yelena V Budovskaya ◽  
Jasper Rine ◽  
Paul K Herman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Rna Polymerase ◽  
Stationary Phase ◽  
Yeast Cell ◽  
Rna Polymerase Ii ◽  
Transcriptional Response ◽  
Nutrient Deprivation ◽  
Ras Signaling ◽  
Rna Polymerase Ii Holoenzyme

Abstract Saccharomyces cerevisiae cells enter into a distinct resting state, known as stationary phase, in response to specific types of nutrient deprivation. We have identified a collection of mutants that exhibited a defective transcriptional response to nutrient limitation and failed to enter into a normal stationary phase. These rye mutants were isolated on the basis of defects in the regulation of YGP1 expression. In wild-type cells, YGP1 levels increased during the growth arrest caused by nutrient deprivation or inactivation of the Ras signaling pathway. In contrast, the levels of YGP1 and related genes were significantly elevated in the rye mutants during log phase growth. The rye defects were not specific to this YGP1 response as these mutants also exhibited multiple defects in stationary phase properties, including an inability to survive periods of prolonged starvation. These data indicated that the RYE genes might encode important regulators of yeast cell growth. Interestingly, three of the RYE genes encoded the Ssn/Srb proteins, Srb9p, Srb10p, and Srb11p, which are associated with the RNA polymerase II holoenzyme. Thus, the RNA polymerase II holoenzyme may be a target of the signaling pathways responsible for coordinating yeast cell growth with nutrient availability.

scholarly journals The Ras/PKA Signaling Pathway May Control RNA Polymerase II Elongation via the Spt4p/Spt5p Complex in Saccharomyces cerevisiae

Genetics ◽  
2003 ◽  
Vol 165 (3) ◽  
pp. 1059-1070
Author(s):  
Susie C Howard ◽  
Arelis Hester ◽  
Paul K Herman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Signaling Pathway ◽  
Elongation Factor ◽  
Ras Signaling ◽  
Elongation Step ◽  
Rna Polymerase Ii Transcription

Abstract The Ras signaling pathway in Saccharomyces cerevisiae controls cell growth via the cAMP-dependent protein kinase, PKA. Recent work has indicated that these effects on growth are due, in part, to the regulation of activities associated with the C-terminal domain (CTD) of the largest subunit of RNA polymerase II. However, the precise target of these Ras effects has remained unknown. This study suggests that Ras/PKA activity regulates the elongation step of the RNA polymerase II transcription process. Several lines of evidence indicate that Spt5p in the Spt4p/Spt5p elongation factor is the likely target of this control. First, the growth of spt4 and spt5 mutants was found to be very sensitive to changes in Ras/PKA signaling activity. Second, mutants with elevated levels of Ras activity shared a number of specific phenotypes with spt5 mutants and vice versa. Finally, Spt5p was efficiently phosphorylated by PKA in vitro. Altogether, the data suggest that the Ras/PKA pathway might be directly targeting a component of the elongating polymerase complex and that this regulation is important for the normal control of yeast cell growth. These data point out the interesting possibility that signal transduction pathways might directly influence the elongation step of RNA polymerase II transcription.

scholarly journals Fibronectin has a dual role in locomotion and anchorage of primary chick fibroblasts and can promote entry into the division cycle.

1982 ◽  
Vol 93 (2) ◽  
pp. 402-410 ◽  
Author(s):  
J R Couchman ◽  
D A Rees ◽  
M R Green ◽  
C G Smith
Keyword(s):  
Focal Adhesion ◽  
Focal Adhesions ◽  
Growth Cycle ◽  
Culture Conditions ◽  
Biological Functions ◽  
Macromolecular Synthesis ◽  
Natural Factor ◽  
Motile Cells ◽  
Chick Heart ◽  
Gold Marker

Fibronectin (FN), which is already known to be a natural factor for fibroblast spreading on substrata, has now been shown to be essential for two distinct types of adhesion with different biological functions in chick heart fibroblasts, namely adhesion directed toward locomotion and toward stationary anchorage for growth. Manipulation of culture conditions and the use of antisera of differing specificities has demonstrated that both exogenous and cell-derived FN are important in each process. The organization of the fibronectin-containing matrix differs between the two states. Immunoelectron microscopy with a colloidal gold marker reveals the presence of small membrane-associated plaques of fibronectin in motile cells with associated submembranous specialization. A fibrillar matrix containing fibronectin is dominant in nonmotile, growing fibroblasts. The development of focal adhesions for stationary anchorage can be dramatically enhanced by addition of cell-derived FN at an appropriate stage, and this promotes entry into the growth cycle. New macromolecular synthesis in addition to FN is necessary for focal adhesion development but not for locomotion.

scholarly journals Prohibitins Regulate Membrane Protein Degradation by the m-AAA Protease in Mitochondria

1999 ◽  
Vol 19 (5) ◽  
pp. 3435-3442 ◽  
Author(s):  
Gregor Steglich ◽  
Walter Neupert ◽  
Thomas Langer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Membrane Proteins ◽  
Eukaryotic Cells ◽  
Mitochondrial Morphology ◽  
Regulatory Effect ◽  
Inner Membrane ◽  
Functional Activities ◽  
Native Molecular Mass ◽  
Affect Cell Growth

ABSTRACT Prohibitins comprise a protein family in eukaryotic cells with potential roles in senescence and tumor suppression. Phb1p and Phb2p, members of the prohibitin family in Saccharomyces cerevisiae, have been implicated in the regulation of the replicative life span of the cells and in the maintenance of mitochondrial morphology. The functional activities of these proteins, however, have not been elucidated. We demonstrate here that prohibitins regulate the turnover of membrane proteins by the m-AAA protease, a conserved ATP-dependent protease in the inner membrane of mitochondria. The m-AAA protease is composed of the homologous subunits Yta10p (Afg3p) and Yta12p (Rca1p). Deletion ofPHB1 or PHB2 impairs growth of Δyta10 or Δyta12 cells but does not affect cell growth in the presence of the m-AAA protease. A prohibitin complex with a native molecular mass of approximately 2 MDa containing Phb1p and Phb2p forms a supercomplex with them-AAA protease. Proteolysis of nonassembled inner membrane proteins by the m-AAA protease is accelerated in mitochondria lacking Phb1p or Phb2p, indicating a negative regulatory effect of prohibitins on m-AAA protease activity. These results functionally link members of two conserved protein families in eukaryotes to the degradation of membrane proteins in mitochondria.

scholarly journals l-Pyroglutamate Spontaneously Formed from l-Glutamate Inhibits Growth of the Hyperthermophilic Archaeon Sulfolobus solfataricus

2001 ◽  
Vol 67 (8) ◽  
pp. 3650-3654 ◽  
Author(s):  
Chan B. Park ◽  
Sun Bok Lee ◽  
Dewey D. Y. Ryu
Keyword(s):  
Cell Growth ◽  
Growth Inhibition ◽  
Yeast Extract ◽  
Formation Rate ◽  
Sulfolobus Solfataricus ◽  
Methionine Sulfoxide ◽  
Inhibitory Effect ◽  
Culture Conditions ◽  
Culture Broth ◽  
Cell Growth Inhibition

ABSTRACT Identification of physiological and environmental factors that limit efficient growth of hyperthermophiles is important for practical application of these organisms to the production of useful enzymes or metabolites. During fed-batch cultivation of Sulfolobus solfataricus in medium containing l-glutamate, we observed formation of l-pyroglutamic acid (PGA). PGA formed spontaneously from l-glutamate under culture conditions (78°C and pH 3.0), and the PGA formation rate was much higher at an acidic or alkaline pH than at neutral pH. It was also found that PGA is a potent inhibitor of S. solfataricus growth. The cell growth rate was reduced by one-half by the presence of 5.1 mM PGA, and no growth was observed in the presence of 15.5 mM PGA. On the other hand, the inhibitory effect of PGA on cell growth was alleviated by addition of l-glutamate or l-aspartate to the medium. PGA was also produced from the l-glutamate in yeast extract; the PGA content increased to 8.5% (wt/wt) after 80 h of incubation of a yeast extract solution at 78°C and pH 3.0. In medium supplemented with yeast extract, cell growth was optimal in the presence of 3.0 g of yeast extract per liter, and higher yeast extract concentrations resulted in reduced cell yields. The extents of cell growth inhibition at yeast extract concentrations above the optimal concentration were correlated with the PGA concentration in the culture broth. Although other structural analogues ofl-glutamate, such as l-methionine sulfoxide, glutaric acid, succinic acid, and l-glutamic acid γ-methyl ester, also inhibited the growth of S. solfataricus, the greatest cell growth inhibition was observed with PGA. We also observed that unlike other glutamate analogues,N-acetyl-l-glutamate enhanced the growth of S. solfataricus. This compound was stable under cell culture conditions, and replacement of l-glutamate with N-acetyl-l-glutamate in the medium resulted in increased cell density.

Colicin E5 Ribonuclease Domain Cleaves Saccharomyces cerevisiae tRNAs Leading to Impairment of the Cell Growth

2009 ◽  
Vol 145 (4) ◽  
pp. 461-466 ◽  
Author(s):  
T. Ogawa ◽  
M. Hidaka ◽  
K. Kohno ◽  
H. Masaki
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth

scholarly journals The Effects of Culture Conditions on the Secretion of Human Lysozyme by Saccharomyces cerevisiae A2-1-1A

1989 ◽  
Vol 53 (10) ◽  
pp. 2687-2694
Author(s):  
Kimihisa Ichikawa ◽  
Koji Komiya ◽  
Katsunori Suzuki ◽  
Tadaatsu Nakahara ◽  
Yoshifumi Jigami
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Culture Conditions ◽  
Human Lysozyme

Multiple elements and auto-repression regulate Rox1, a repressor of hypoxic genes in Saccharomyces cerevisiae.

Genetics ◽  
1995 ◽  
Vol 139 (3) ◽  
pp. 1149-1158 ◽  
Author(s):  
J Deckert ◽  
R Perini ◽  
B Balasubramanian ◽  
R S Zitomer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Blot Analysis ◽  
Lacz Fusion ◽  
Upstream Region ◽  
Fusion Construct ◽  
Gel Retardation ◽  
Gal1 Promoter ◽  
Rna Blot Analysis

Abstract The ROX1 gene encodes a heme-induced repressor of hypoxic genes in yeast. Using RNA blot analysis and a ROX1/lacZ fusion construct that included the ROX1 upstream region and only the first codon, we discovered that Rox1 represses its own expression. Gel-retardation experiments indicated that Rox1 was capable of binding to its own upstream region. Overexpression of Rox1 from the inducible GAL1 promoter was found to be inhibitory to cell growth. Also, we found that, as reported previously, Hap1 is partially responsible for heme-induction of ROX1, but, in addition, it also may play a role in ROX1 repression in the absence of heme. There is a second repressor of anaerobic ROX1 expression that requires the general repressor Tup1/Ssn6 for its function.

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