Studies of Cell Growth on TiO2 Nanotubes

2012 ◽  
Vol 620 ◽  
pp. 325-329 ◽  
Author(s):  
Nur Hidayati Ahmad Barudin ◽  
Srimala Sreekantan ◽  
Ong Ming Thong ◽  
Lam Kit Lay
Keyword(s):  
Cancer Cell Line ◽  
Cellular Responses ◽  
Cell Interaction ◽  
Electrochemical Anodization ◽  
X Ray Diffraction ◽  
Cell Adherence ◽  
Anodization Voltage ◽  
X Ray ◽  
Mcf 7

The in vitro cell response was investigated on flat Ti surface vs nanostructured TiO2 nanotube surface. The titanium dioxide nanotube layers were prepared by electrochemical anodization of Ti in ethylene glycol, 5 wt% NH4F and 1ml H2O2. The nanotube layered structure and morphology were characterized using X-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM). The diameter and the length of the nanotubes are found to increase with anodization voltage. Hs27 and breast cancer cell line MCF-7 were used for cell interaction studies. Different surfaces of titanium show variation in term of growth and viability of cells. Different cell type also show different cellular responses to these surfaces. Titanium nanotube with tube diameter 90 nm promoted normal cell adherence and spreading but killed the cancer cells. The detail of the observation is discussed thoroughly in this paper.

scholarly journals Synthesis, Crystal Structure, and Biological Activity ofcis/transAmide Rotomers of (Z)-N′-(2-Oxoindolin-3-ylidene)formohydrazide

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hatem A. Abdel-Aziz ◽  
Hazem A. Ghabbour ◽  
Wagdy M. Eldehna ◽  
Maha M. Qabeel ◽  
Hoong-Kun Fun
Keyword(s):  
Crystal Structure ◽  
Human Tumor ◽  
Cancer Cell Line ◽  
Monoclinic Space Group ◽  
Moderate Activity ◽  
Standard Drug ◽  
X Ray ◽  
H Nmr ◽  
Mcf 7

(Z)-N′-(2-Oxoindolin-3-ylidene)formohydrazide (2) was synthesized by the reaction of (Z)-3-hydrazonoindolin-2-one (1) with formic acid under reflux. The structure of2was characterized by IR, Mass,1H NMR, and X-ray crystal structure determination. Interestingly, compound2appeared in DMSO-d6ascisandtransamide rotomers in 25% and 75%, respectively. The X-ray analysis showed theZgeometrical isomer of2around –C=N– forcisandtransamide rotomers. The crystal of2belongs to monoclinic, space groupP21/c, witha=4.5206(1) Å,b=22.4747(7) Å,c=17.3637(5) Å,β=103.752(1)°,Z=8,V=1713.57(8) Å3,Dc=1.467 Mg m−3,μ=0.11 mm−1,F(000)=784,R=0.047, andwR=0.123for 3798 observed reflections withI>2σ(I). Compound2exhibited a moderate activity in its antimicrobial evaluation againstE. coliandP. aeruginosaand a good activity againstS. aureusclose to that of the standard drug ciprofloxacin. Thein vitroanticancer activity of2was evaluated against two human tumor cell lines, namely, HepG2 hepatocellular carcinoma and MCF-7 breast cancer. HepG2 cancer cell line was more susceptible to compound2than MCF-7.

scholarly journals Curcumin-Loaded Mixed Micelles: Preparation, Characterization, and In Vitro Antitumor Activity

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Suping Ji ◽  
Xiao Lin ◽  
Enjiang Yu ◽  
Chengyang Dian ◽  
Xiong Yan ◽  
...  
Keyword(s):  
Mixed Micelles ◽  
Drug Loading ◽  
Molecular State ◽  
X Ray Diffraction ◽  
X Ray ◽  
Release Property ◽  
Mean Size ◽  
Xrd Patterns ◽  
Mcf 7

The objective of this study was to prepare curcumin-loaded mixed Soluplus/TPGS micelles (Cur-TPGS-PMs) for oral administration. The Cur-TPGS-PMs showed a mean size of 65.54 ± 2.57 nm, drug encapsulation efficiency over 85%, and drug loading of 8.17%. The Cur-TPGS-PMs were found to be stable in various pH media (pH 1.2 for 2 h, pH 6.8 for 2 h, and pH 7.4 for 6 h). The X-ray diffraction (XRD) patterns illustrated that curcumin was in the amorphous or molecular state within PMs. The In vitro release test indicated that Cur-TPGS-PMs possessed a significant sustained-release property. The cell viability in MCF-7 cells was found to be relatively lower in Cur-TPGS-PM-treated cells as compared to free Cur-treated cells. CLSM imaging revealed that mixed micelles were efficiently absorbed into the cytoplasm region of MCF-7 cells. Therefore, Cur-TPGS-PMs could have the significant value for the chronic breast cancer therapy.

Reactions of 2-cyano-3-ferrocenylacrylonitrile with malononitrile: formation of 4-ferrocenylpyridine-3,5-dicarbonitrile derivatives and sodium polymeric complexes containing carbanionic ligands

2014 ◽  
Vol 86 (11) ◽  
pp. 1839-1852 ◽  
Author(s):  
Elena Ivanovna Klimova ◽  
Marcos Martínez García ◽  
Jessica Jazmin Sánchez García ◽  
Teresa Ramírez Apan ◽  
Andrei V. Churakov ◽  
...  
Keyword(s):  
Human Tumor ◽  
Tumor Cell Lines ◽  
X Ray Diffraction ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines ◽  
X Ray ◽  
Biological Specificity ◽  
Polymeric Complexes ◽  
Mcf 7

Abstract The reactions of 2-cyano-3-ferrocenylacrylonitrile with malononitrile in a EtOH/H2O or MeOH/H2O medium in the presence of Na2CO3 afforded 6-alkoxy-2-amino-4-ferrocenylpyridine-3,5-dicarbonitriles 3a,b (multi-component condensation), 6-alkoxy-2-amino-4-ferrocenyl-3-ferrocenylmethyl-3,4-dihydropyridine-3,5-dicarbonitriles 4a,b (multi-component cyclodimerization) and Na+ polymeric complexes: {[Na+(2-ferrocenyl(tetracyano)propenyl)–L]∞5a,b and [Na+(2-amino-3,5-dicyano-4-ferrocenyl-6-pyridyl-dicyanomethyl)–L]∞6a,b, where L = ethanol, methanol. Complexes with L = acetonitrile, dimethylformamide, acetone, ethyl acetate were prepared by recrystallization. The structures of the compounds 3b, 4b and Na+ polymeric complexes were established by the spectroscopic data and X-ray diffraction analysis. Two compounds 3a and 4a were tested in vitro against six human tumor cell lines U-251, PC-3, K-562, HCT-15, MCF-7 and SKLU-1 to assess their in vitro antitumor activity. The results suggest biological specificity towards PC-3, K-562 and HCT-15 cells for compound 3a, and towards PC-3 cell for compound 4a at doses of 50 μM, which are lower than cis-platin.

Mixed Micelle-Template Route to Mesoporous Silica

2007 ◽  
Vol 7 (11) ◽  
pp. 3819-3822 ◽  
Author(s):  
You-Hwan Son ◽  
Man Park ◽  
Sang Tae Kim ◽  
Jin-Ho Choy
Keyword(s):  
Mesoporous Silica ◽  
Polyethylene Oxide ◽  
Mixed Micelle ◽  
Weight Ratio ◽  
Mesoporous Structure ◽  
X Ray Diffraction ◽  
X Ray ◽  
Diffraction Patterns ◽  
Mcf 7

Mesoporous silica materials were prepared through a novel mixed micelle-template method which was employed by alkyl polyethylene oxide (C16E20 and C2-ceramide. X-ray diffraction patterns clearly showed the formation of mesoporous silica by contribution of mixed micelle-template up to 3/1 weight ratio (C16E20/C2-ceramide). TEM and N2 adsorption isotherms analyses indicated that the mesoporous structure was maintained even after encased C2-ceramides. However, when the weight ratio of C16E20/C2-ceramide exceeds 2/2, less ordered and irregular pore structure was observed. According to the in-vitro experiment on cancer cells such as MCF-7, HOS, and HepG2, the simultaneously encapsulated C2-ceramide shows apoptosis. Therefore, the present results could provide a new method for mesoporous material as drug delivery system.

scholarly journals Tersone A-G, New Pyridone Alkaloids from the Deep-Sea Fungus Phomopsis tersa

Marine Drugs ◽  
2019 ◽  
Vol 17 (7) ◽  
pp. 394 ◽  
Author(s):  
Shan-Chong Chen ◽  
Zhao-Ming Liu ◽  
Hai-Bo Tan ◽  
Yu-Chan Chen ◽  
Sai-Ni Li ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
A549 Cell ◽  
Deep Sea ◽  
X Ray Diffraction ◽  
X Ray ◽  
Spectroscopic Analyses ◽  
Ic50 Values ◽  
Mic Value ◽  
Mcf 7

Four phenylfuropyridone racemates, (±)-tersones A-C and E (1–3, 5), one phenylpyridone racemate, (±)-tersone D (4), one new pyridine alkaloid, tersone F (6), single new phenylfuropyridone, tersone G (7) and two known analogs 8 and 9 were isolated from the deep-sea fungus Phomopsis tersa. Their structures and absolute configurations were characterized on the basis of comprehensive spectroscopic analyses, single-crystal X-ray diffraction experiments, and electronic circular dichroism (ECD) calculations. Moreover, compounds 1–9 were evaluated for in vitro antimicrobial and cytotoxic activity. Compounds 5b and 8b exhibited antibacterial activity against S. aureus with the MIC value of 31.5 μg/mL, while compound 5b showed cytoxic activities against SF-268, MCF-7, HepG-2 and A549 cell lines with IC50 values of 32.0, 29.5, 39.5 and 33.2 μM, respectively.

scholarly journals In Vitro Cytotoxicity and Morphological Assessments of GO-ZnO against the MCF-7 Cells: Determination of Singlet Oxygen by Chemical Trapping

Nanomaterials ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 539 ◽  
Author(s):  
Fozia Shaheen ◽  
Muhammad Aziz ◽  
Mahvish Fatima ◽  
Muhammad Khan ◽  
Faisal Ahmed ◽  
...  
Keyword(s):  
Chemical Properties ◽  
In Vitro Cytotoxicity ◽  
Dependent Manner ◽  
X Ray Diffraction ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Potential Applicability ◽  
Mcf 7

Graphene-based materials have attracted considerable interest owing to their distinctive characteristics, such as their biocompatibility in terms of both their physical and intrinsic chemical properties. The use of nanomaterials with graphene as a biocompatible agent has increased due to an uptick in dedication from biomedical investigators. Here, GO-ZnO was characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), ultraviolet-visible (UV-Vis) spectroscopy, energy dispersive X-ray analysis (EDAX), and Raman spectroscopy for structural, morphological, and elemental analysis. The toxic extent of GO-ZnO was noted by a methyl-thiazole-tetrazolium (MTT), while cellular morphology was observed towards the MCF-7 cells using an inverted microscope at magnification 40×. The cytotoxic effect of GO-ZnO investigated the cell viability reduction in a dose-dependent manner, as well as prompted the cell demise/destruction in an apoptotic way. Moreover, statistical analysis was performed on the experimental outcomes, with p-values < 0.05 kept as significant to elucidate the results. The generation of reactive oxygen species (ROS) demonstrated the potential applicability of graphene in tumor treatment. These key results attest to the efficacy of GO-ZnO nanocomposites as a substantial candidate for breast malignancy treatment.

scholarly journals Biocompatibility Computation of Muscle Cells on Polyhedral Oligomeric Silsesquioxane-Grafted Polyurethane Nanomatrix

Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2966
Author(s):  
Touseef Amna ◽  
Mallick Shamshi Hassan ◽  
Mohamed H. El-Newehy ◽  
Tariq Alghamdi ◽  
Meera Moydeen Abdulhameed ◽  
...  
Keyword(s):  
Fiber Diameter ◽  
Polyhedral Oligomeric Silsesquioxane ◽  
Cellular Responses ◽  
X Ray Diffraction ◽  
X Ray ◽  
Physicochemical Investigation ◽  
Biomimetic Scaffolds ◽  
Myoblast Cell ◽  
Oligomeric Silsesquioxane

This study was performed to appraise the biocompatibility of polyhedral oligomeric silsesquioxane (POSS)-grafted polyurethane (PU) nanocomposites as potential materials for muscle tissue renewal. POSS nanoparticles demonstrate effectual nucleation and cause noteworthy enhancement in mechanical and thermal steadiness as well as biocompatibility of resultant composites. Electrospun, well-aligned, POSS-grafted PU nanofibers were prepared. Physicochemical investigation was conducted using several experimental techniques, including scanning electron microscopy, energy dispersive X-ray spectroscopy, electron probe microanalysis, Fourier transform infrared spectroscopy, and X-ray diffraction pattern. Adding POSS molecules to PU did not influence the processability and morphology of the nanocomposite; however, we observed an obvious mean reduction in fiber diameter, which amplified specific areas of the POSS-grafted PU. Prospective biomedical uses of nanocomposite were also appraised for myoblast cell differentiation in vitro. Little is known about C2C12 cellular responses to PU, and there is no information regarding their interaction with POSS-grafted PU. The antimicrobial potential, anchorage, proliferation, communication, and differentiation of C2C12 on PU and POSS-grafted PU were investigated in this study. In conclusion, preliminary nanocomposites depicted superior cell adhesion due to the elevated free energy of POSS molecules and anti-inflammatory potential. These nanofibers were non-hazardous, and, as such, biomimetic scaffolds show high potential for cellular studies and muscle regeneration.

scholarly journals Mixed natural arylolefin–quinoline platinum(II) complexes: synthesis, structural characterization and in vitro cytotoxicity studies

2018 ◽  
Vol 74 (12) ◽  
pp. 1732-1743
Author(s):  
Nguyen Thi Thanh Chi ◽  
Pham Van Thong ◽  
Truong Thi Cam Mai ◽  
Luc Van Meervelt
Keyword(s):  
Double Bond ◽  
Cytotoxic Effect ◽  
In Vitro Cytotoxicity ◽  
Quinoline Derivative ◽  
X Ray Diffraction ◽  
Hep G2 ◽  
X Ray ◽  
Cytotoxicity Studies ◽  
Mcf 7

Five new platinum(II) complexes bearing a eugenol and a quinoline derivative, namely [η2-4-allyl-2-methoxy-1-(propoxycarbonylmethoxy)benzene]-trans-dichlorido(quinoline-κN)platinum(II), [PtCl2(C15H20O4)(C9H7N)], (2), {η2-4-allyl-2-methoxy-1-[(propan-2-yloxy)carbonylmethoxy]benzene}-trans-dichlorido(quinoline-κN)platinum(II), [PtCl2(C15H19O4)(C9H7N)], (3), [η2-4-allyl-2-methoxy-1-(propoxycarbonylmethoxy)benzene]chlorido(quinolin-8-olato-κ2 N,O)platinum(II), [Pt(C9H6NO)Cl(C15H20O4)], (4), {η2-4-allyl-2-methoxy-1-[(propan-2-yloxy)carbonylmethoxy]benzene}chlorido(quinolin-8-olato-κ2 N,O)platinum(II), [Pt(C9H6NO)Cl(C15H20O4)], (5), and [η2-4-allyl-2-methoxy-1-(propoxycarbonylmethoxy)benzene]chlorido(quinolin-2-carboxylato-κ2 N,O)platinum(II), [Pt(C10H6NO2)Cl(C15H20O4)], (6), have been synthesized and fully characterized spectroscopically. A single-crystal X-ray diffraction study was carried out for complexes (2) and (4)–(6). PrEug [or 4-allyl-2-methoxy-1-(propoxycarbonylmethoxy)benzene] in (2), (4) and (6), and iPrEug (the propan-2-yloxy analogue of PrEug) in (3) and (5) coordinate with PtII at the ethylenic double bond of the allyl group. In (2)–(6), the donor N atom of the amine group occupies a trans position with respect to the double bond. A comparison of the IC50 values of 0.38–29.23 µM for (2)–(6) with cisplatin, as well as other platinum(II) complexes, indicates an excellent in vitro cytotoxicity against the KB, LU, Hep-G2 and MCF-7 cancer cell lines, with the highest cytotoxic effect (IC50 = 0.38–1.99 µM) being for complexes (4) and (5) bearing a quinolin-8-olate ligand.

scholarly journals Hormone Anchored Metal Complexes. 1. Synthesis, Structure, Spectroscopy and In Vitro Antitumor Activity of Testosterone Acetate Thiosemicarbazone and its Metal Complexes

1999 ◽  
Vol 6 (3) ◽  
pp. 177-182 ◽  
Author(s):  
Anupa Murugkar ◽  
Bindu Unnikrishnan ◽  
Subhash Padhye ◽  
Ramesh Bhonde ◽  
Simon Teat ◽  
...  
Keyword(s):  
Metal Complexes ◽  
Cancer Cell Line ◽  
Platinum Complexes ◽  
Human Breast Cancer Cell ◽  
In Vitro Activity ◽  
Human Breast ◽  
X Ray ◽  
Steroid Derivative ◽  
Mcf 7

Testosterone acetate thiosemicarbazone (TATSC, 17-β -hydroxyandrost-4-one acetate thiosemicarbazone) was synthesized and characterized by single crystal X-ray structure determination. The copper and platinum complexes of this steroid derivative were synthesized and characterized by spectroscopy and electrochemiatry. The in vitro activity of these compounds against human breast cancer cell line MCF-7 was tested. The highest activity was found for the [Pt(TATSC)Cl2] followed by [Cu(TATSC)Cl2] and the ligand in compariosn with cisplatin.

scholarly journals In vitro targeting and selective killing of mcf-7 and colo320dm cells by 5-fluorouracil anchored to carboxylated SWCNTs and MWCNTs

2021 ◽  
Vol 32 (6) ◽  
Author(s):  
Rutuja V. Kamble ◽  
Somnath D. Bhinge ◽  
Shrinivas K. Mohite ◽  
Dheeraj S. Randive ◽  
Mangesh A. Bhutkar
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Functional Group ◽  
Drug Loading ◽  
Loading Capacity ◽  
X Ray Diffraction ◽  
X Ray ◽  
Swcnts And Mwcnts ◽  
Mcf 7

AbstractThe intention of the present work was to synthesize the f-MWCNT and f-SWCNT terminated with proper functional group, loading of 5-Flurouracil and to perform cytotoxic activity. Functionalization of MWCNTs and SWCNTs was achieved through the acid treatment (H2SO4 + HNO3). 5-flurouracil was loaded into the prepared functionalized CNTs, thereafter; in vitro drug loading capacity and % drug release were calculated. Also the prepared f-CNTs, 5-flurouracil loaded CNTs were distinguished by using SEM, TGA, DSC, X-ray diffraction, Raman and FTIR spectroscopy. MCF-7 and COLO320DM cells were treated with selected concentrations of 5-FU loaded f-MWCNTs and f-SWCNTs to estimate the cytotoxic activity. It was observed that 5-FU loaded f-SWCNTs showed good activity against selected cell lines than others. Moreover, apoptosis percentage was reported to be 84.46 ± 4.3515 and 92.78 ± 2.6549 for 5-FU loaded f-SWCNTs against MCF-7 and COLO320DM cells respectively. It is evident from the results that the prepared drug loaded CNTs have comparable antitumor activity in cancer cell lines.

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