Curcumin-Loaded Mixed Micelles: Preparation, Characterization, and In Vitro Antitumor Activity

The objective of this study was to prepare curcumin-loaded mixed Soluplus/TPGS micelles (Cur-TPGS-PMs) for oral administration. The Cur-TPGS-PMs showed a mean size of 65.54 ± 2.57 nm, drug encapsulation efficiency over 85%, and drug loading of 8.17%. The Cur-TPGS-PMs were found to be stable in various pH media (pH 1.2 for 2 h, pH 6.8 for 2 h, and pH 7.4 for 6 h). The X-ray diffraction (XRD) patterns illustrated that curcumin was in the amorphous or molecular state within PMs. The In vitro release test indicated that Cur-TPGS-PMs possessed a significant sustained-release property. The cell viability in MCF-7 cells was found to be relatively lower in Cur-TPGS-PM-treated cells as compared to free Cur-treated cells. CLSM imaging revealed that mixed micelles were efficiently absorbed into the cytoplasm region of MCF-7 cells. Therefore, Cur-TPGS-PMs could have the significant value for the chronic breast cancer therapy.

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In vitro targeting and selective killing of mcf-7 and colo320dm cells by 5-fluorouracil anchored to carboxylated SWCNTs and MWCNTs

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-021-06540-8 ◽

2021 ◽

Vol 32 (6) ◽

Author(s):

Rutuja V. Kamble ◽

Somnath D. Bhinge ◽

Shrinivas K. Mohite ◽

Dheeraj S. Randive ◽

Mangesh A. Bhutkar

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Functional Group ◽

Drug Loading ◽

Loading Capacity ◽

X Ray Diffraction ◽

X Ray ◽

Swcnts And Mwcnts ◽

Mcf 7

AbstractThe intention of the present work was to synthesize the f-MWCNT and f-SWCNT terminated with proper functional group, loading of 5-Flurouracil and to perform cytotoxic activity. Functionalization of MWCNTs and SWCNTs was achieved through the acid treatment (H2SO4 + HNO3). 5-flurouracil was loaded into the prepared functionalized CNTs, thereafter; in vitro drug loading capacity and % drug release were calculated. Also the prepared f-CNTs, 5-flurouracil loaded CNTs were distinguished by using SEM, TGA, DSC, X-ray diffraction, Raman and FTIR spectroscopy. MCF-7 and COLO320DM cells were treated with selected concentrations of 5-FU loaded f-MWCNTs and f-SWCNTs to estimate the cytotoxic activity. It was observed that 5-FU loaded f-SWCNTs showed good activity against selected cell lines than others. Moreover, apoptosis percentage was reported to be 84.46 ± 4.3515 and 92.78 ± 2.6549 for 5-FU loaded f-SWCNTs against MCF-7 and COLO320DM cells respectively. It is evident from the results that the prepared drug loaded CNTs have comparable antitumor activity in cancer cell lines.

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Reactions of 2-cyano-3-ferrocenylacrylonitrile with malononitrile: formation of 4-ferrocenylpyridine-3,5-dicarbonitrile derivatives and sodium polymeric complexes containing carbanionic ligands

Pure and Applied Chemistry ◽

10.1515/pac-2014-0611 ◽

2014 ◽

Vol 86 (11) ◽

pp. 1839-1852 ◽

Cited By ~ 1

Author(s):

Elena Ivanovna Klimova ◽

Marcos Martínez García ◽

Jessica Jazmin Sánchez García ◽

Teresa Ramírez Apan ◽

Andrei V. Churakov ◽

...

Keyword(s):

Human Tumor ◽

Tumor Cell Lines ◽

X Ray Diffraction ◽

Human Tumor Cell ◽

Human Tumor Cell Lines ◽

X Ray ◽

Biological Specificity ◽

Polymeric Complexes ◽

Mcf 7

Abstract The reactions of 2-cyano-3-ferrocenylacrylonitrile with malononitrile in a EtOH/H2O or MeOH/H2O medium in the presence of Na2CO3 afforded 6-alkoxy-2-amino-4-ferrocenylpyridine-3,5-dicarbonitriles 3a,b (multi-component condensation), 6-alkoxy-2-amino-4-ferrocenyl-3-ferrocenylmethyl-3,4-dihydropyridine-3,5-dicarbonitriles 4a,b (multi-component cyclodimerization) and Na+ polymeric complexes: {[Na+(2-ferrocenyl(tetracyano)propenyl)–L]∞5a,b and [Na+(2-amino-3,5-dicyano-4-ferrocenyl-6-pyridyl-dicyanomethyl)–L]∞6a,b, where L = ethanol, methanol. Complexes with L = acetonitrile, dimethylformamide, acetone, ethyl acetate were prepared by recrystallization. The structures of the compounds 3b, 4b and Na+ polymeric complexes were established by the spectroscopic data and X-ray diffraction analysis. Two compounds 3a and 4a were tested in vitro against six human tumor cell lines U-251, PC-3, K-562, HCT-15, MCF-7 and SKLU-1 to assess their in vitro antitumor activity. The results suggest biological specificity towards PC-3, K-562 and HCT-15 cells for compound 3a, and towards PC-3 cell for compound 4a at doses of 50 μM, which are lower than cis-platin.

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Bio-Based Nanoparticles as a Carrier of β-Carotene: Production, Characterisation and In Vitro Gastrointestinal Digestion

Molecules ◽

10.3390/molecules25194497 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4497

Author(s):

Beatriz S. Afonso ◽

Ana G. Azevedo ◽

Catarina Gonçalves ◽

Isabel R. Amado ◽

Eugénio C. Ferreira ◽

...

Keyword(s):

Encapsulation Efficiency ◽

In Vitro Digestion ◽

X Ray Diffraction ◽

X Ray ◽

Transmission Electron ◽

Mean Size ◽

Average Size ◽

Size Controlled ◽

Β Carotene

β-carotene loaded bio-based nanoparticles (NPs) were produced by the solvent-displacement method using two polymers: zein and ethylcellulose. The production of NPs was optimised through an experimental design and characterised in terms of average size and polydispersity index. The processing conditions that allowed to obtain NPs (<100 nm) were used for β-carotene encapsulation. Then β-carotene loaded NPs were characterised in terms of zeta potential and encapsulation efficiency. Transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction analysis were performed for further morphological and chemical characterisation. In the end, a static in vitro digestion following the INFOGEST protocol was performed and the bioaccessibility of β-carotene encapsulated in both NPs was determined. Results show that the best conditions for a size-controlled production with a narrow size distribution are lower polymer concentrations and higher antisolvent concentrations. The encapsulation of β-carotene in ethylcellulose NPs resulted in nanoparticles with a mean average size of 60 ± 9 nm and encapsulation efficiency of 74 ± 2%. β-carotene loaded zein-based NPs resulted in a mean size of 83 ± 8 nm and encapsulation efficiency of 93 ± 4%. Results obtained from the in vitro digestion showed that β-carotene bioaccessibility when encapsulated in zein NPs is 37 ± 1%, which is higher than the value of 8.3 ± 0.1% obtained for the ethylcellulose NPs.

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Studies of Cell Growth on TiO2 Nanotubes

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.620.325 ◽

2012 ◽

Vol 620 ◽

pp. 325-329 ◽

Cited By ~ 1

Author(s):

Nur Hidayati Ahmad Barudin ◽

Srimala Sreekantan ◽

Ong Ming Thong ◽

Lam Kit Lay

Keyword(s):

Cancer Cell Line ◽

Cellular Responses ◽

Cell Interaction ◽

Electrochemical Anodization ◽

X Ray Diffraction ◽

Cell Adherence ◽

Anodization Voltage ◽

X Ray ◽

Mcf 7

The in vitro cell response was investigated on flat Ti surface vs nanostructured TiO2 nanotube surface. The titanium dioxide nanotube layers were prepared by electrochemical anodization of Ti in ethylene glycol, 5 wt% NH4F and 1ml H2O2. The nanotube layered structure and morphology were characterized using X-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM). The diameter and the length of the nanotubes are found to increase with anodization voltage. Hs27 and breast cancer cell line MCF-7 were used for cell interaction studies. Different surfaces of titanium show variation in term of growth and viability of cells. Different cell type also show different cellular responses to these surfaces. Titanium nanotube with tube diameter 90 nm promoted normal cell adherence and spreading but killed the cancer cells. The detail of the observation is discussed thoroughly in this paper.

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Green Synthesis and Biomedical Applications of ZnO Nanoparticles: Role of PEGylated-ZnO Nanoparticles as Doxorubicin Drug Carrier against MDA-MB-231(TNBC) Cells Line

Crystals ◽

10.3390/cryst11040344 ◽

2021 ◽

Vol 11 (4) ◽

pp. 344

Author(s):

Madiha Batool ◽

Shazia Khurshid ◽

Walid M. Daoush ◽

Sabir Ali Siddique ◽

Tariq Nadeem

Keyword(s):

Breast Cancer ◽

Electron Microscopy ◽

Zno Nanoparticles ◽

Drug Loading ◽

Loading Capacity ◽

X Ray Diffraction ◽

Zno Nps ◽

X Ray ◽

Drug Loading Efficiency

The present study aimed to develop the synthesis of zinc oxide nanoparticles (ZnO-NPs) using the green method, with Aloe barbadensis leaf extract as a stabilizing and capping agent. In vitro antitumor cytotoxic activity, as well as the surface-functionalization of ZnO-NPs and their drug loading capacity against doxorubicin (DOX) and gemcitabine (GEM) drugs, were also studied. Morphological and structural properties of the produced ZnO-NPs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersion X-ray diffraction (EDX), UV-Vis spectrophotometry, Fourier-transform infrared analysis (FTIR), and X-ray diffraction (XRD). The prepared ZnO-NPs had a hexagonal shape and average particle size of 20–40 nm, with an absorption peak at 325 nm. The weight and atomic percentages of zinc (50.58% and 28.13%) and oxygen (26.71% and 60.71%) were also determined by EDAX (energy dispersive x-ray analysis) compositional analysis. The appearance of the FTIR peak at 3420 m–1 confirmed the synthesis of ZnO-NPs. The drug loading efficiency (LE) and loading capacity (LC) of unstabilized and PEGylated ZnO-NPs were determined by doxorubicin (DOX) and gemcitabine (GEM) drugs. DOX had superior LE 65% (650 mg/g) and higher LC 32% (320 mg/g) than GEM LE 30.5% (30 mg/g) and LC 16.25% (162 mg/g) on ZnO-NPs. Similar observation was observed in the case of PEG-ZnO-NPs, where DOX had enhanced LE 68% (680 mg/g) and LC 35% (350) mg/g in contrast to GEM, which had LE and LC values of 35% (350 mg/g) and 19% (190 mg/g), respectively. Therefore, DOX was chosen to encapsulate nanoparticles, along with the untreated nanoparticles, to check their in vitro antiproliferative potential against the triple-negative breast cancer (TNBC) cell line (MDA-MB-231) through the MTT (3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide) assay. This drug delivery strategy implies that the PEGylated biogenically synthesized ZnO-NPs occupy an important position in chemotherapeutic drug loading efficiency and can improve the therapeutic techniques of triple breast cancer.

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rhBMP-2 Induces Immature Muscular Tissue to Differentiate into Bone-Like Tissue In Vitro

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.587.103 ◽

2013 ◽

Vol 587 ◽

pp. 103-108

Author(s):

Tatsuhide Hayashi ◽

Kentaro Yoshihara ◽

Mayu Kawase ◽

Akimichi Mieki ◽

Hiroyasu Kataoka ◽

...

Keyword(s):

Extracellular Matrix ◽

Muscular Tissue ◽

Sprague Dawley ◽

X Ray Diffraction ◽

X Ray ◽

Electron Probe Micro Analyzer ◽

Mineral Deposition ◽

Partially Observed ◽

Xrd Patterns

The aim of this study is to induce bone from immature muscular tissue in vitro using recombinant human BMP (rhBMP)-2 and expanded polytetrafluoroethylene (ePTFE) as a scaffold. Commercially available rhBMP-2 was used in this experiment. IMTs were harvested from the forelimbs of 20th Sprague-Dawley embryonic rats and placed into a homogenizer with 10ng/μl of rhBMP-2 and then homogenized. The homogenized IMT was placed on ePTFE and cultured for 2 weeks. The analyses of histological observation, electron probe micro analyzer (EPMA), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were carried out following culture. The bone-like tissue, which was made up of osteoblast-like cells and osteoids, was partially observed by H-E staining. Moreover, strong mineral deposition was observed in the extracellular matrix by von Kossa staining. Ca, P and O were detected in the extracellular matrix by EPMA and were confirmed to be at almost the same position based on the findings of synchronized images. XRD patterns and FTIR spectra of specimen were found to have typical hydroxyapatite crystal peaks and spectra, respectively. These results suggest that rhBMP-2 induced IMT differentiation into bone-like tissue in vitro.

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Mixed Micelle-Template Route to Mesoporous Silica

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2007.037 ◽

2007 ◽

Vol 7 (11) ◽

pp. 3819-3822 ◽

Cited By ~ 1

Author(s):

You-Hwan Son ◽

Man Park ◽

Sang Tae Kim ◽

Jin-Ho Choy

Keyword(s):

Mesoporous Silica ◽

Polyethylene Oxide ◽

Mixed Micelle ◽

Weight Ratio ◽

Mesoporous Structure ◽

X Ray Diffraction ◽

X Ray ◽

Diffraction Patterns ◽

Mcf 7

Mesoporous silica materials were prepared through a novel mixed micelle-template method which was employed by alkyl polyethylene oxide (C16E20 and C2-ceramide. X-ray diffraction patterns clearly showed the formation of mesoporous silica by contribution of mixed micelle-template up to 3/1 weight ratio (C16E20/C2-ceramide). TEM and N2 adsorption isotherms analyses indicated that the mesoporous structure was maintained even after encased C2-ceramides. However, when the weight ratio of C16E20/C2-ceramide exceeds 2/2, less ordered and irregular pore structure was observed. According to the in-vitro experiment on cancer cells such as MCF-7, HOS, and HepG2, the simultaneously encapsulated C2-ceramide shows apoptosis. Therefore, the present results could provide a new method for mesoporous material as drug delivery system.

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Tersone A-G, New Pyridone Alkaloids from the Deep-Sea Fungus Phomopsis tersa

Marine Drugs ◽

10.3390/md17070394 ◽

2019 ◽

Vol 17 (7) ◽

pp. 394 ◽

Cited By ~ 7

Author(s):

Shan-Chong Chen ◽

Zhao-Ming Liu ◽

Hai-Bo Tan ◽

Yu-Chan Chen ◽

Sai-Ni Li ◽

...

Keyword(s):

Antibacterial Activity ◽

A549 Cell ◽

Deep Sea ◽

X Ray Diffraction ◽

X Ray ◽

Spectroscopic Analyses ◽

Ic50 Values ◽

Mic Value ◽

Mcf 7

Four phenylfuropyridone racemates, (±)-tersones A-C and E (1–3, 5), one phenylpyridone racemate, (±)-tersone D (4), one new pyridine alkaloid, tersone F (6), single new phenylfuropyridone, tersone G (7) and two known analogs 8 and 9 were isolated from the deep-sea fungus Phomopsis tersa. Their structures and absolute configurations were characterized on the basis of comprehensive spectroscopic analyses, single-crystal X-ray diffraction experiments, and electronic circular dichroism (ECD) calculations. Moreover, compounds 1–9 were evaluated for in vitro antimicrobial and cytotoxic activity. Compounds 5b and 8b exhibited antibacterial activity against S. aureus with the MIC value of 31.5 μg/mL, while compound 5b showed cytoxic activities against SF-268, MCF-7, HepG-2 and A549 cell lines with IC50 values of 32.0, 29.5, 39.5 and 33.2 μM, respectively.

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Preparation and Characterization of ZnSe/SiO2 Nanocomposite

Journal of Metastable and Nanocrystalline Materials ◽

10.4028/www.scientific.net/jmnm.23.71 ◽

2005 ◽

Vol 23 ◽

pp. 71-74 ◽

Cited By ~ 4

Author(s):

Fantao Kong ◽

Xi Yao ◽

Min Qiang Wang ◽

Haiqing Jiang ◽

Yun Peng Wang

Keyword(s):

Absorption Spectra ◽

Photoelectron Spectroscopy ◽

Sol Gel ◽

Silica Matrix ◽

X Ray Diffraction ◽

X Ray ◽

Synthesis Conditions ◽

As Doping ◽

Mean Size ◽

Xrd Patterns

Semiconductor ZnSe nanocrystals (NCs)-doped SiO2 glasses (designated as ZnSe/SiO2 nanocomposites) were prepared successfully by using sol-gel method and in-situ growth technique. The nanocomposite was characterized by X-ray diffraction (XRD) patterns, UV-Vis absorption spectra and X-ray Photoelectron Spectroscopy (XPS). The XRD results show that ZnSe NCs is zinc blende structure and the mean size is smaller than 5nm in diameter. UV-Vis absorption spectra reveals that the size of the NCs is influenced by synthesis conditions such as doping concentration and thermal treatment, and XPS together with XRD can prove that silica matrix plays an important role in enhancing the NCs’ stability.

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In Vitro Cytotoxicity and Morphological Assessments of GO-ZnO against the MCF-7 Cells: Determination of Singlet Oxygen by Chemical Trapping

Nanomaterials ◽

10.3390/nano8070539 ◽

2018 ◽

Vol 8 (7) ◽

pp. 539 ◽

Cited By ~ 6

Author(s):

Fozia Shaheen ◽

Muhammad Aziz ◽

Mahvish Fatima ◽

Muhammad Khan ◽

Faisal Ahmed ◽

...

Keyword(s):

Chemical Properties ◽

In Vitro Cytotoxicity ◽

Dependent Manner ◽

X Ray Diffraction ◽

X Ray ◽

Vis Spectroscopy ◽

Potential Applicability ◽

Mcf 7

Graphene-based materials have attracted considerable interest owing to their distinctive characteristics, such as their biocompatibility in terms of both their physical and intrinsic chemical properties. The use of nanomaterials with graphene as a biocompatible agent has increased due to an uptick in dedication from biomedical investigators. Here, GO-ZnO was characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), ultraviolet-visible (UV-Vis) spectroscopy, energy dispersive X-ray analysis (EDAX), and Raman spectroscopy for structural, morphological, and elemental analysis. The toxic extent of GO-ZnO was noted by a methyl-thiazole-tetrazolium (MTT), while cellular morphology was observed towards the MCF-7 cells using an inverted microscope at magnification 40×. The cytotoxic effect of GO-ZnO investigated the cell viability reduction in a dose-dependent manner, as well as prompted the cell demise/destruction in an apoptotic way. Moreover, statistical analysis was performed on the experimental outcomes, with p-values < 0.05 kept as significant to elucidate the results. The generation of reactive oxygen species (ROS) demonstrated the potential applicability of graphene in tumor treatment. These key results attest to the efficacy of GO-ZnO nanocomposites as a substantial candidate for breast malignancy treatment.

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