Characterization of Bone Cell Behaviors on Collagen Grafted Hydroxyapatite Surfaces

In order to enhance bone cell adhesion on hydroxyapatite (HA), collagen was used as a surface-grafting material on HA substrates because the collagen is a major constituent of connective tissues and has been regarded as one of the most excellent coating materials for bone bonding. First, HA disks (10mmΦ x 1mm) were prepared and then collagen was immobilbized on the HA surface using an 3-APTES coupling agent on HA disk surfaces. MC3T3-E1 osteoblasts were seeded on the collagen-grafted and non-grated HA disks and cultured in a Dulbecco’s modified eagle medium containing 10% fetal bovine serum for 4 hrs to evaluate the cell adhesion on the HA samples. The osteoblasts on the collagen-grafted sample were more spread than those on the non-grafted sample. It is believed that collagen-grafted HA surface provides suitable sites for cell attaching due to the high biocompatibility of collagen

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Immobilization of Collagen on Hydroxyapatite and Its Interation with Cells

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.330-332.781 ◽

2007 ◽

Vol 330-332 ◽

pp. 781-784 ◽

Cited By ~ 3

Author(s):

K.H. Jung ◽

Inn Kyu Kang ◽

Sung Min Kim ◽

Myun Whan Ahn ◽

Suk Young Kim

Keyword(s):

Cell Adhesion ◽

Bone Cells ◽

Fetal Bovine Serum ◽

Major Constituent ◽

Connective Tissues ◽

Coating Materials ◽

Removal Torque ◽

3T3 Fibroblasts ◽

Fetal Bovine ◽

Nih 3T3

The adhesion of bone cells on substrate materials is generally measured by the removal torque and/or contact area between bone and implants. In this study, collagen was used as a surfacegrafting material on hydroxyapatite (HA) substrates to enhance the cell adhesion because the collagen is a major constituent of connective tissues and has been regarded as one of the most excellent coating materials for bone bonding. First, HA disks (10mmΦ x 1mm) were prepared and then collagen was immobilbized on the HA surface using an 3-APTES coupling agent to improve the adhesiveness of cells on HA disk surfaces. NIH 3T3 fibroblasts were seeded on the collagengrafted and non-grated HA disks and cultured in a Dulbecco’s modified eagle medium containing 10% fetal bovine serum for 3 hrs to evaluate the cell adhesion on the HA samples. The fibroblasts on the collagen-grafted sample were more spread than those on the non-grafted sample. It is believed that collagen-grafted HA surface provides suitable sites for cell attaching due to the high biocompatibility of collagen.

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A Collagen Grafting on Hydroxyapatite to Enhancement of Bone Cell Attachment

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.396-398.41 ◽

2008 ◽

Vol 396-398 ◽

pp. 41-45

Author(s):

D.W. Lee ◽

E.J. Lee ◽

Sung Su Chun ◽

Myun Whan Ahn ◽

I.W. Song ◽

...

Keyword(s):

Collagen Type ◽

Cell Attachment ◽

Collagen Type I ◽

Bone Cell ◽

Major Constituent ◽

Type I ◽

Connective Tissues ◽

Coating Materials ◽

Attached Cell ◽

Scanning Electron

A collagen material was chemically grafted on hydroxyapatite (HA) to enhance bone cell attachment because the collagen is a major constituent of connective tissues and has been regarded as one of the most excellent coating materials for bone bonding. First, HA disks were prepared with 12mm diameter and 1mm thickness. And then collagen (type I) was immobilbized on the HA surface using a 3-APTES coupling agent on HA disk surfaces. MC3T3-E1 osteoblasts were seeded on the collagen-grafted and non-grated HA disks and cultured for 4 hrs to evaluate the cell adhesion on the HA discs. The Attached cell morphology on discs was observed with a fluorescent optical microscopy (FOM) and a scanning electron microscopy (SEM). The osteoblasts on the collagen-grafted sample were more spread than those on the non-grafted sample. It is believed that collagen-grafted HA surface provides suitable sites for cell attaching due to the high biocompatibility of collagen.

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