A Collagen Grafting on Hydroxyapatite to Enhancement of Bone Cell Attachment

2008 ◽  
Vol 396-398 ◽  
pp. 41-45
Author(s):  
D.W. Lee ◽  
E.J. Lee ◽  
Sung Su Chun ◽  
Myun Whan Ahn ◽  
I.W. Song ◽  
...  
Keyword(s):  
Collagen Type ◽  
Cell Attachment ◽  
Collagen Type I ◽  
Bone Cell ◽  
Major Constituent ◽  
Type I ◽  
Connective Tissues ◽  
Coating Materials ◽  
Attached Cell ◽  
Scanning Electron

A collagen material was chemically grafted on hydroxyapatite (HA) to enhance bone cell attachment because the collagen is a major constituent of connective tissues and has been regarded as one of the most excellent coating materials for bone bonding. First, HA disks were prepared with 12mm diameter and 1mm thickness. And then collagen (type I) was immobilbized on the HA surface using a 3-APTES coupling agent on HA disk surfaces. MC3T3-E1 osteoblasts were seeded on the collagen-grafted and non-grated HA disks and cultured for 4 hrs to evaluate the cell adhesion on the HA discs. The Attached cell morphology on discs was observed with a fluorescent optical microscopy (FOM) and a scanning electron microscopy (SEM). The osteoblasts on the collagen-grafted sample were more spread than those on the non-grafted sample. It is believed that collagen-grafted HA surface provides suitable sites for cell attaching due to the high biocompatibility of collagen.

Bacterial Endotoxin Inhibits Migration, Attachment, and Orientation of Human Gingival Fibroblasts in vitro and Delays Collagen Gel Contraction

1987 ◽  
Vol 66 (9) ◽  
pp. 1449-1455 ◽  
Author(s):  
S. Pitaru ◽  
M. Soldinger ◽  
D. Madgar ◽  
Z. Metzger
Keyword(s):  
Collagen Type ◽  
Cell Attachment ◽  
Collagen Gel ◽  
Collagen Type I ◽  
Human Gingival Fibroblasts ◽  
Bacterial Endotoxin ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Collagen Gels

The purpose of this study was to assess the effect of endotoxin adsorbed to dental surfaces and to collagen type I on the migration, attachment, and orientation of human gingival fibroblasts (HGF). Transversely cut porcine tooth root slices (RS), 200 μm thick, were prepared. Half of the RS obtained were partially demineralized in EDTA. Half of the demineralized and non-demineralized RS were incubated with 400 μg/mL of endotoxin for 24 hr, whereas the other half were maintained in PBS and served as controls. Experimental and control RS were placed on confluent layers of HFG and cultured for six days. Cell migration toward and cell attachment to the periphery of the RS and the formation of oriented cell sheets were assessed by means of photographic techniques. Additionally, six-day-old cultures were fixed and processed for SEM observation. In separate experiments, the effect of endotoxin on cell attachment to collagen type I and on contraction of three-dimensional collagen gels was assessed. It was found that: (i) bacterial endotoxin inhibited migration and attachment of HGF to both demineralized and non-demineralized cementum and interfered with the development of oriented cellular structure ; (ii) the inhibitory effect was significantly more pronounced for non-demineralized than for demineralized cementum; (iii) the morphology of HGF attached to endotoxin-treated dental surfaces was altered compared with that of their controls; and (iv) bacterial endotoxin inhibited cell attachment to collagen type I and delayed the contraction of collagen gel.

scholarly journals The bioactivity of composite Fmoc-RGDS-collagen gels

2014 ◽  
Vol 2 (9) ◽  
pp. 1222-1229 ◽  
Author(s):  
Ricardo M. Gouveia ◽  
Roanne R. Jones ◽  
Ian W. Hamley ◽  
Che J. Connon
Keyword(s):  
Structural Properties ◽  
Collagen Type ◽  
Cell Attachment ◽  
Collagen Type I ◽  
Type I ◽  
Collagen Gels ◽  
Mechanical And Structural Properties ◽  
Self Assembled

Collagen type-I gels incorporating self-assembled Fmoc-RGDS during polymerisation become functionalised, providing a scaffold for enhanced cell attachment and survival, and with improved mechanical and structural properties.

scholarly journals Poly(NaSS) Functionalization Modulates the Conformation of Fibronectin and Collagen Type I To Enhance Osteoblastic Cell Attachment onto Ti6Al4V

Langmuir ◽  
2014 ◽  
Vol 30 (31) ◽  
pp. 9477-9483 ◽  
Author(s):  
Helena P. Felgueiras ◽  
Sven D. Sommerfeld ◽  
N. Sanjeeva Murthy ◽  
Joachim Kohn ◽  
Véronique Migonney
Keyword(s):  
Collagen Type ◽  
Cell Attachment ◽  
Collagen Type I ◽  
Osteoblastic Cell ◽  
Type I

Characterization of Bone Cell Behaviors on Collagen Grafted Hydroxyapatite Surfaces

2007 ◽  
Vol 361-363 ◽  
pp. 1143-1146
Author(s):  
S. Chun ◽  
D.H. Shin ◽  
I.K. Kang ◽  
M.W. Ahn ◽  
Suk Young Kim
Keyword(s):  
Cell Adhesion ◽  
Fetal Bovine Serum ◽  
Bone Cell ◽  
Major Constituent ◽  
Surface Grafting ◽  
Connective Tissues ◽  
Coating Materials ◽  
Cell Behaviors ◽  
Fetal Bovine

In order to enhance bone cell adhesion on hydroxyapatite (HA), collagen was used as a surface-grafting material on HA substrates because the collagen is a major constituent of connective tissues and has been regarded as one of the most excellent coating materials for bone bonding. First, HA disks (10mmΦ x 1mm) were prepared and then collagen was immobilbized on the HA surface using an 3-APTES coupling agent on HA disk surfaces. MC3T3-E1 osteoblasts were seeded on the collagen-grafted and non-grated HA disks and cultured in a Dulbecco’s modified eagle medium containing 10% fetal bovine serum for 4 hrs to evaluate the cell adhesion on the HA samples. The osteoblasts on the collagen-grafted sample were more spread than those on the non-grafted sample. It is believed that collagen-grafted HA surface provides suitable sites for cell attaching due to the high biocompatibility of collagen

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