Interface Function Design and Bone-Regenerative Engineering of Biomimetic Biomaterials by Supersonic Treatment Using Electrolyzed Water

2014 ◽  
Vol 631 ◽  
pp. 241-246 ◽  
Author(s):  
Toshiyuki Akazawa ◽  
Masaru Murata ◽  
Y. Minamida ◽  
Md. Arafat Kabir ◽  
M. Ito ◽  
...  
Keyword(s):  
Interface Design ◽  
Bone Growth ◽  
Porous Ceramics ◽  
Parietal Bone ◽  
Spherical Particles ◽  
Human Teeth ◽  
Electrolyzed Water ◽  
Water Ph ◽  
Interface Function ◽  
Bone Growth Factor

As interface design of autotransportation materials and bioactive ceramics by supersonic treatment, human teeth-originated granules and commercial hydroxyapatite (HAp) were partially dissolved in different acid solutions, such as electrolyzed water and HNO3aqueous solution to control bio-absorption, adsorption-release of bone growth factor and anti-bacterial characteristics. Human teeth were pulverized with cooling, dissolved in strongly acidic electrolyzed water (pH 2.6-3.1) or 2.0% HNO3solution to obtain demineralized dentin matrix granules. For supersonic or stirring demineralization in the acidic electrolyzed water, dissolution efficiencies (DE) were 3-12% and dentinal tubules with 1-2μm were found, while for supersonic demineralization in the HNO3solution, DE was attained to the highest value of 86%. When HAp was dissolved with stirring in the acidic electrolyzed water, DE of porous ceramics was lower than that of spherical particles due to smaller specific surface area and good crystallinity. For supersonic dissolution of porous ceramics in the acidic electrolyzed water, DE was 30% and extension of grain boundary and micro-crack were observed. Concerning supersonic irradiation of the electolyzed water to parietal bone in rats by using ultrasonic scaler, enlargement and propagation of micro-crack were recognized on the hard tissues.

Functionalized Calcium Phosphate-based Nanoparticles for the Treatment of Osteoporosis

2005 ◽  
Vol 873 ◽  
Author(s):  
Balasundaram G ◽  
Sato M ◽  
Webster TJ
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Amorphous Calcium Phosphate ◽  
Bone Growth ◽  
Osteoporotic Bone ◽  
Amino Groups ◽  
Calcium Phosphate Nanoparticles ◽  
Bioactive Agents ◽  
Bone Growth Factor

AbstractIn an effort to decrease the number of problems associated with osteoporosis, the long-term goal of the present study is to design calcium phosphate-based nanoparticles that specifically attach to areas of low bone density and once attached, allow for the targeted release of bioactive agents that can quickly increase bone formation. Efforts are focused on nanoparticles of calcium phosphate-based materials since they are similar in size and chemistry to the major inorganic components of bone. As a first step in this research, the objective of the present study was to synthesize nanoparticles of crystalline hydroxyapatite (or HA) and amorphous calcium phosphate. Crystalline HA is stable under physiological fluids and, thus, will release embedded bioactive agents slowly. Alternatively, amorphous calcium phosphate is highly biodegradable and will, thus, release embedded bioactive agents quickly. A further objective of the present study was to functionalize such inorganic biodegradable materials with amino groups which would allow for the subsequent attachment of entities to direct such nanoparticles to osteoporotic bone and increased bone formation once attached. One promising approach to direct the nanoparticles to osteoporotic bone is to attach antibodies to pentosidine on the nanoparticles since pentosidine is present in higher amounts in osteoporotic compared to healthy bone. A promising approach to increase bone growth once nanoparticles attach to osteoporotic bone, is to embed nanoparticles with regions of the bone growth factor: bone morphogenic protein-2 (or BMP-2). Results of this study demonstrated the successful synthesis of both crystalline HA and amorphous calcium phosphate nanoparticles. Furthermore, results showed that these nanoparticles can be functionalized with versatile amino groups. In this manner, this study takes the first steps toward utilizing calcium phosphate based nanoparticles to reverse bone loss associated with osteoporosis.

Stimulation of bone growth factor synthesis in human osteoblasts and fibroblasts after extracorporeal shock wave application

2010 ◽  
Vol 131 (3) ◽  
pp. 303-309 ◽  
Author(s):  
Joerg Hausdorf ◽  
Birte Sievers ◽  
Marcus Schmitt-Sody ◽  
Volkmar Jansson ◽  
Markus Maier ◽  
...  
Keyword(s):  
Shock Wave ◽  
Growth Factor ◽  
Bone Growth ◽  
Human Osteoblasts ◽  
Extracorporeal Shock Wave ◽  
Bone Growth Factor ◽  
Stimulation Of

Augmentation of Tendon Healing in an Intraarticular Bone Tunnel with Use of a Bone Growth Factor

2001 ◽  
Vol 29 (6) ◽  
pp. 689-698 ◽  
Author(s):  
Kyle Anderson ◽  
Aruna M. Seneviratne ◽  
Kazutaka Izawa ◽  
Brent L. Atkinson ◽  
Hollis G. Potter ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Growth ◽  
Cruciate Ligament ◽  
Tendon Healing ◽  
Tendon Graft ◽  
Bone Tunnel ◽  
Resonance Imaging ◽  
Load To Failure ◽  
Anterior Cruciate ◽  
Bone Growth Factor

We hypothesized that an exogenous bone growth factor could augment healing of a tendon graft in a bone tunnel in a rabbit anterior cruciate ligament-reconstruction model. Seventy rabbits underwent bilateral anterior cruciate ligament reconstructions with a semitendinosus tendon graft. One limb received a collagen sponge carrier vehicle containing a mixture of bone-derived proteins while the contralateral limb was treated with either no sponge or a sponge without bone-derived proteins. The reconstruction was evaluated at 2, 4, or 8 weeks with histologic, biomechanical, and magnetic resonance imaging analysis. Histologic analysis demonstrated that specimens treated with bone-derived proteins had a more consistent, dense interface tissue and closer apposition of new bone to the graft, with occasional formation of a fibrocartilaginous interface, when compared with control specimens. The treated specimens had significantly higher load-to-failure rates than did control specimens. Treatment with bone-derived proteins resulted in an average increase in tensile strength of 65%. The treated specimens were stronger than control specimens at each time point, but the difference was greatest at 8 weeks. On the basis of signal characteristics and new bone formation, magnetic resonance imaging was useful for predicting which limb was treated, the site of failure, and the limbs with higher load-to-failure values. This study demonstrates the potential for augmenting tendon healing in an intraarticular bone tunnel using an osteoinductive growth factor.

SYNTHETIC BIOMATERIALS FOR RECONSTRUCTIVE SURGERY WITH THE ADDITION OF BMP-2 AND ERYTHROPOIETIN

2020 ◽  
pp. 50-52
Author(s):  
F. Senatov ◽  
A. Chubrik ◽  
A. Zimina ◽  
N. Strukova ◽  
M. Krivozubov ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Growth Factor ◽  
High Molecular Weight ◽  
Reconstructive Surgery ◽  
Bone Growth ◽  
Synthetic Materials ◽  
Bone Growth Factor ◽  
Ultra High Molecular Weight

Synthetic materials based on Ultra-High Molecular Weight PolyEthylene (UHMWPE), PolyEtherEtherKetone (PEEK) and PolyLActide (PLA) with the addition of recombinant bone growth factor BMP-2 and erythropoietin have been developed, which have high biocompatibility and osteoinductive properties.

scholarly journals Accelerated Bone Induction of Adult Rat Compact Bone Plate Scratched by Ultrasonic Scaler Using Acidic Electrolyzed Water

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3347
Author(s):  
Mamata Shakya ◽  
Masaru Murata ◽  
Kenji Yokozeki ◽  
Toshiyuki Akazawa ◽  
Hiroki Nagayasu ◽  
...  
Keyword(s):  
Ultrasonic Treatment ◽  
Compact Bone ◽  
Parietal Bone ◽  
New Combination ◽  
Graft Material ◽  
Skull Bone ◽  
Vascular Perfusion ◽  
Electrolyzed Water ◽  
Combination Technique ◽  
Ultrasonic Scaler

Fresh compact bone, the candidate graft material for bone regeneration, is usually grafted for horizontal bone augmentation. However, the dense calcified structure inhibits the release of growth factors and limits cellular and vascular perfusion. We aimed to create mechano-chemically altered dense skull bone by ultrasonic treatment, along with partial demineralization using commercially available acidic electrolyzed water (AEW). The parietal skull bone of an 11-month-old Wistar rat was exposed and continuously treated with a piezoelectric ultrasonic scaler tip for 1 min, using AEW (pH 2.3) or distilled water (DW, pH 5.6) as irrigants. Treated parietal bone was removed, cut into plates (5 × 5 × 1 mm3), grafted into the back subcutaneous tissues of syngeneic rats, and explanted at 1, 2, and 3 weeks. AEW bone showed an irregular surface, deep nano-microcracks, and decalcified areas. SEM-EDS revealed small amounts of residual calcium content in the AEW bone (0.03%) compared to the DW bone (0.86%). In the animal assay, the AEW bone induced bone at 2 weeks. Histomorphometric analysis showed that the area of new bone in the AEW bone at 2 and 3 weeks was significantly larger. This new combination technique of AEW-demineralization with ultrasonic treatment will improve the surface area and three-dimensional (3D) architecture of dense bone and accelerate new bone synthesis.

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