In Vivo Evaluation of Chelate-Setting Cement Fabricated from Hydroxyapatite Including Bone Minerals Using a Rabbit’s Tibia Model

2014 ◽  
Vol 631 ◽  
pp. 402-407
Author(s):  
Mamoru Aizawa ◽  
Yuki Chibu ◽  
Kohei Nagata ◽  
Toshiisa Konishi ◽  
Ken Ishii ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Inositol Phosphate ◽  
The Novel ◽  
Hard Tissue ◽  
Biological Apatite ◽  
Bone Minerals ◽  
Surface Modified ◽  
Cement Specimen

Hydroxyapatite (HAp) is one of components of bone and teeth, and has an osteoconductivity. Thus, the HAp has been used as biomaterials for bone graftings. We have succeeded in development of the novel chelate-setting calcium-phosphate cement (CPC) using pure HAp particles surface-modified with inositol phosphate (IP6). While, biological apatite presented in bone and teeth of mammals contains various ions: Na+, K+, Mg2+, Cl-, F- and CO32-, in addition to Ca2+ and PO43- ions. In this work, in order to create the chelate-setting CPC with enhanced osteoconductivity, the above-mentioned biological apatite powder (hereafter, bone HAp), instead of pure HAp, was used as a starting powder for fabrication of the chelate-setting cement. The biocompatibility of the resulting chelate-setting bone HAp cement (hereafter, IP6-bone HAp cement) was examined using a rabbit’s tibia model. When the living reaction to hard tissue was histologically examined after 4 weeks implantation, we could observe that newly-formed bone directly bonded to the surface of the specimen. The newly-formed bone was also present around the cement specimen. The amounts of newly-formed bone around IP6-bone HAp cement was about 1.5 times those around IP6-pure HAp cement without bone minerals. The above findings demonstrate that the present IP6-bone HAp cements are one of the promising candidates as novel CPC with enhanced osteoconductivity.

Development of Bioresorbable Calcium-Phosphate Cements Hybridized with Gelatin Particles and their In Vivo Evaluation Using Pig’s Tibia Model

2014 ◽  
Vol 631 ◽  
pp. 397-401 ◽  
Author(s):  
Keishi Kiminami ◽  
Kento Matsuoka ◽  
Kohei Nagata ◽  
Toshiisa Konishi ◽  
Michiyo Honda ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Inositol Phosphate ◽  
Setting Time ◽  
Chelating Agent ◽  
Sulfate Solution ◽  
Initial Setting Time ◽  
Initial Setting ◽  
Preliminary Study ◽  
Surface Modified

Novel bioresorbable calcium-phosphate cement (CPC) with anti-washout property was developed by adding thermally cross-linked gelatin particles as pore generator into a CPC. The CPC was composed of α-tricalcium phosphate (α-TCP) and surface-modified hydroxyapatite (HAp) with inositol phosphate as a chelating agent (IP6-HAp). The bioresorbable CPC hybridized with gelatin particles was successfully fabricated by mixing the aqueous sodium chondroitin sulfate solution including Na2HPO4 and the pre-mixed powders composed of α-TCP (72 mass%), IP6-HAp (18 mass%), and the gelatin particles (10 mass%). The hybridized CPC paste showed initial setting time (IST) of 5 minutes and exhibited anti-washout property. Compressive strength after setting for 24 h reached to 4.2 MPa. An in vivo preliminary study using pig’s tibia model demonstrated that the hybridized CPC could be easily injected and set promptly without washout. In addition, no fragmentation in the specimens was observed after 8 weeks implantation. Moreover, a histological observation (Villanueva bone stain) revealed that almost 80% of the hybridized CPC specimens were resorbed and that immature bones were formed inside the specimens.

Research and Development of Calcium Phosphate Cement

2007 ◽  
Vol 353-358 ◽  
pp. 2267-2270
Author(s):  
Chang Sheng Liu
Keyword(s):  
Calcium Phosphate ◽  
Research And Development ◽  
Calcium Phosphate Cement ◽  
Ambient Conditions ◽  
Hard Tissue ◽  
Shape Characteristic ◽  
Different Types ◽  
Tissue Defects

Hydroxyapatite (HAP)-forming calcium phosphate cement (CPC), due to the high biocompatibility, easy-to-shape characteristic, and the capacity to self-setting under ambient conditions, has been widely used for the repair of hard tissue defects. To satisfy the different clinical need, some modified CPC, including porous CPC, fast-biodegradable CPC, injectable CPC, water-resistant CPC, and rhBMP-2/CPC, have been designed and fabricated in recent years. This paper attempts to give an overview of different types of CPC that have being developed at the present time. Meanwhile, the application perspective of these modified CPC is also explored.

Comparative Study on Bioresorbability of Chelate-Setting Cements with Various Calcium-Phosphate Phase Using Rabbit Model

2012 ◽  
Vol 529-530 ◽  
pp. 167-172 ◽  
Author(s):  
Toshiisa Konishi ◽  
Shuhei Takahashi ◽  
Minori Mizumoto ◽  
Michiyo Honda ◽  
Koki Kida ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Inositol Phosphate ◽  
Rabbit Model ◽  
The Novel ◽  
Calcium Phosphate Cements ◽  
Phosphate Phase ◽  
Setting Mechanism ◽  
Calcium Phosphate Phase

We have developed novel calcium-phosphate cements (CPCs) based on the chelate-setting mechanism of inositol phosphate (IP6) using hydroxyapatite (HAp), β-tricalcium phosphate (β-TCP) and α-TCP as starting materials. These cements (IP6-HAp, IP6-β-TCP and IP6-α-TCP cements) have different bioresorbability due to the chemical composition of starting materials. In the present study, biocompatibility and bioresorbability of the above three cements and commercially available cement (Biopex®-R) was histologically evaluated in vivo using rabbit model for 4, 8, and 24 weeks, in addition to their dissolution in vitro. The dissolution of these cements increased in the order of IP6-HAp, IP6-β-TCP and IP6-α-TCP cements. The newly-formed bones were directly in contact with both the IP6-HAp and Biopex®-R cement specimens. As for the IP6-β-TCP and IP6-α-TCP cements, newly-formed bones were formed time-dependently slightly apart from the cement specimens. Resorption rate for Biopex®-R, IP6-HAp, IP6-β-TCP, and IP6-α-TCP cements after 24 weeks implantation were of 7.2, 5.0, 13.7, and 16.2%, respectively, compared to original cements. The present chelate-setting cements with different bioresorbability are promising candidates for application as the novel CPCs.

Preparation of α-Tricalcium Phosphate Powders Surface-Modified with Inositol Phosphate for Cement Fabrication

2014 ◽  
Vol 631 ◽  
pp. 113-118
Author(s):  
Toshiisa Konishi ◽  
Michiyo Honda ◽  
Tomohiko Yoshioka ◽  
Satoshi Hayakawa ◽  
Mamoru Aizawa
Keyword(s):  
Ball Milling ◽  
Tricalcium Phosphate ◽  
Single Phase ◽  
Inositol Phosphate ◽  
Potential Candidate ◽  
Preparation Process ◽  
The Novel ◽  
Powder Preparation ◽  
Setting Mechanism ◽  
Surface Modified

We have previously developed biodegradable β-tricalcium phosphate (β-TCP) cement based on the chelate-setting mechanism of inositol phosphate (IP6). The β-TCP cement powder for the cement fabrication was prepared via a novel powder preparation process, in which the starting β-TCP powders were prepared by simultaneous ball-milling and surface-modification in the IP6 solution. In the present study, the novel powder preparation process was applied to an α-TCP powder, and effect of milling time and beads size for ball-milling on the material properties of the α-TCP powders was investigated. The α-TCP powder ball-milled in 1000 ppm IP6 solution for 4 h with 2 mm-diameter beads was composed of single phase α-TCP with the smallest particle size of 2.2 µm. Dissolution of 4 h-milled α-TCP powder was approximately twice higher than that of starting α-TCP powder before ball-milling. The α-TCP powder with high dissolution property prepared via the novel powder preparation process is potential candidate for fabrication of the chelate-setting cement.

Biocompatibility of Silver-Containing Calcium-Phosphate Cements with Anti-Bacterial Properties

2014 ◽  
Vol 631 ◽  
pp. 107-112
Author(s):  
Yusuke Shimizu ◽  
Yusuke Kawanobe ◽  
Toshiisa Konishi ◽  
Nobuyuki Kanzawa ◽  
Michiyo Honda ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Inositol Phosphate ◽  
Ultrasonic Spray Pyrolysis ◽  
Nominal Composition ◽  
Calcium Phosphate Cements ◽  
Ultrasonic Spray ◽  
Polystyrene Plate ◽  
Tcp Phase ◽  
Relative Cell Viability ◽  
Cement Specimen

We have previously synthesized silver-containing hydroxyapatite (Ag-HAp) powders by an ultrasonic spray-pyrolysis (USSP) technique. On the other hand, we have successfully fabricated novel calcium-phosphate cements (CPCs) composed of mainly β-tricalcium phosphate (β-TCP) phase with anti-washout property (hereafter, β-TCP cement), which was set on the basis of chelate-bonding ability of inositol phosphate (IP6). In this study, we developed novel CPCs with both anti-bacterial and anti-washout properties by adding the Ag-HAp powder into the above β-TCP cements, and examined their anti-bacterial property and cytotoxicity. The Ag-HAp powders with Ag contents of 0, 2, and 5 mol% as a nominal composition were synthesized by an USSP technique. The raw powder for β-TCP cement was prepared by ball-milling the commercially-available β-TCP powder in the IP6 solution. The Ag-HAp/β-TCP powders were prepared by mixing Ag-HAp powder and β-TCP cement powder at a ratio of 25:75 in mass. The Ag-HAp/β-TCP cement was fabricated by mixing the above-mentioned Ag-HAp/β-TCP powder and 2.5 mass% Na2HPO4 solution at a powder/liquid ratio of 1/0.3 [g/cm3]. The anti-bacterial property of resulting cements was evaluated using Staphylococcus aureus by biofilm formation test. The Ag-HAp/β-TCP cements containing 2 and 5 mol% Ag showed strong anti-bacterial property among examined specimens. Furthermore, the cytotoxicity of Ag+ ion eluted from these cements was also examined using osteoblastic MC3T3-E1 cells and Transwell® kit. The relative cell viability cultured on each Ag-containing cement specimen was over 80 %, compared with the control (polystyrene plate). These results demonstrate that the present Ag-HAp/β-TCP cements containing 2 mol% Ag are promising one of the candidates as CPCs with both anti-bacterial property and biocompatibility.

PL DLLA Calcium Phosphate Composite Combined with Macroporous Calcium Phosphate Cement MCPC® for New Surgical Technologies Combining Resorbable Osteosynthesis and Injectable Bone Substitute

2007 ◽  
Vol 361-363 ◽  
pp. 411-414 ◽  
Author(s):  
Gaelle Jouan ◽  
Eric Goyenvalle ◽  
Eric Aguado ◽  
Ronan Cognet ◽  
Françoise Moreau ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Bone Substitute ◽  
Biphasic Calcium Phosphate ◽  
Bone Ingrowth ◽  
The Novel ◽  
Powder Component ◽  
New Generation ◽  
Controlled Hydrolysis

Resorbable osteosynthesis based on PLLA and derivatives will be associated to bone substitute for bone reconstruction. We have performed rand evaluated a composite combining PL DLLA and Biphasic calcium phosphate able to have a), a better controlled hydrolysis in the purpose to preserve on time the mechanical property, and b), for long term efficiency, bone ingrowth at the expense of the osteosynthesis and the associated bone substitute. A new calcium phosphate cement MCPC® was tested with such composite. The novel macroporous calcium phosphate cement MCPC sets to poorly crystalline apatite after mixing the powder component and an aqueous solution. Interconnective macroporosity was induced on time by resorption of one part of the MCPC®. The multiphasic calcium phosphate components in the cement, are resorbed at different rates allowing the replacement by newly formed bone. This study reports the biocompatibility and the interactions of a composite using PL DLLA (Poly [L-Lactide-co-D,L-Lactide] acid) charged with biphasic calcium phosphate granules and a self setting calcium phosphate cement of new generation.

scholarly journals A bone replacement-type calcium phosphate cement that becomes more porous in vivo by incorporating a degradable polymer

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Akiyoshi Shimatani ◽  
Hiromitsu Toyoda ◽  
Kumi Orita ◽  
Yuta Ibara ◽  
Yoshiyuki Yokogawa ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Alginic Acid ◽  
Setting Time ◽  
Zealand White Rabbit ◽  
Control Group ◽  
Degradable Polymer ◽  
Low Viscosity ◽  
Bone Replacement

AbstractThis study investigated whether mixing low viscosity alginic acid with calcium phosphate cement (CPC) causes interconnected porosity in the CPC and enhances bone replacement by improving the biological interactions. Furthermore, we hypothesized that low viscosity alginic acid would shorten the setting time of CPC and improve its strength. CPC samples were prepared with 0, 5, 10, and 20% low viscosity alginic acid. After immersion in acetate buffer, possible porosification in CPC was monitored in vitro using scanning electron microscopy (SEM), and the setting times and compressive strengths were measured. In vivo study was conducted by placing CPC in a hole created on the femur of New Zealand white rabbit. Microcomputed tomography and histological examination were performed 6 weeks after implantation. SEM images confirmed that alginic acid enhanced the porosity of CPC compared to the control, and the setting time and compressive strength also improved. When incorporating a maximum amount of alginic acid, the new bone mass was significantly higher than the control group (P = 0.0153). These biological responses are promising for the translation of these biomaterials and their commercialization for clinic applications.

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