In Vitro Characterization of 3D Beta-Tricalcium Phosphate Scaffolds Reinforced with Phosphate Based-Bioactive Glass for Bone Replacement

Bone tissue engineering is an excellent alternative to reduce bone disorders and conditions, by inducing new functional bone regeneration starting from the synthesis of the biomaterials and then the combination of cell and factor therapy. In the present contribution, the scaffolds were made with a ratio of 80 wt.% of β-TCP and 20 wt. % of phosphate-based bioglass, in addition the phosphate-based bioglass was reinforced with zirconia in different amounts (0, 0.5 and 1.0 mol%) with the aim to reduce the dissolution rate, improve the osteoconduction and the osteogenesis of the bone tissue. The results obtained by μCT of the scaffolds containing zirconia showed a wide pore size distribution between 1.5 and 303 μm. AlamarBlue assays showed that the cell proliferation of MC3T3-E1 preosteoblasts scaffolds were sixfold increase in relation to the number of the initial cells. FE-SEM helped to observe the cauliflower structure of HA and DRX showed that crystalline phases formed after heat treatment were (NaCaPO4 and NaZr5PO4) owing both to the crystallization and combination of the bioglass and β-TCP .

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In-vitro evaluation of thermoplastic starch/ beta-tricalcium phosphate nano-biocomposite in bone tissue engineering

Ceramics International ◽

10.1016/j.ceramint.2021.02.111 ◽

2021 ◽

Author(s):

Marzieh Taherimehr ◽

Reza Bagheri ◽

Masoumeh Taherimehr

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Tricalcium Phosphate ◽

Thermoplastic Starch ◽

In Vitro Evaluation ◽

Beta Tricalcium Phosphate

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Fabrication and characterization of electrospinning/3D printing bone tissue engineering scaffold

RSC Advances ◽

10.1039/c6ra17718b ◽

2016 ◽

Vol 6 (112) ◽

pp. 110557-110565 ◽

Cited By ~ 49

Author(s):

Yinxian Yu ◽

Sha Hua ◽

Mengkai Yang ◽

Zeze Fu ◽

Songsong Teng ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Composite Scaffold ◽

Tissue Engineering Scaffold ◽

Fabrication And Characterization

A composite scaffold was fabricated with a method involving both electrospinning and 3D printing to give microscale pores and good mechanical properties. Biocompatibility and cell infiltration on the scaffold was evaluated by an in vitro study.

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Fabrication and In Vitro Evaluation of 3D Printed Porous Polyetherimide Scaffolds for Bone Tissue Engineering

BioMed Research International ◽

10.1155/2019/2076138 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Xiongfeng Tang ◽

Yanguo Qin ◽

Xinyu Xu ◽

Deming Guo ◽

Wenli Ye ◽

...

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Cell Proliferation ◽

Cell Adhesion ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Porous Scaffold ◽

3D Printed

For bone tissue engineering, the porous scaffold should provide a biocompatible environment for cell adhesion, proliferation, and differentiation and match the mechanical properties of native bone tissue. In this work, we fabricated porous polyetherimide (PEI) scaffolds using a three-dimensional (3D) printing system, and the pore size was set as 800 μm. The morphology of 3D PEI scaffolds was characterized by the scanning electron microscope. To investigate the mechanical properties of the 3D PEI scaffold, the compressive mechanical test was performed via an electronic universal testing system. For the in vitro cell experiment, bone marrow stromal cells (BMSCs) were cultured on the surface of the 3D PEI scaffold and PEI slice, and cytotoxicity, cell adhesion, and cell proliferation were detected to verify their biocompatibility. Besides, the alkaline phosphatase staining and Alizarin Red staining were performed on the BMSCs of different samples to evaluate the osteogenic differentiation. Through these studies, we found that the 3D PEI scaffold showed an interconnected porous structure, which was consistent with the design. The elastic modulus of the 3D PEI scaffold (941.33 ± 65.26 MPa) falls in the range of modulus for the native cancellous bone. Moreover, the cell proliferation and morphology on the 3D PEI scaffold were better than those on the PEI slice, which revealed that the porous scaffold has good biocompatibility and that no toxic substances were produced during the progress of high-temperature 3D printing. The osteogenic differentiation level of the 3D PEI scaffold and PEI slice was equal and ordinary. All of these results suggest the 3D printed PEI scaffold would be a potential strategy for bone tissue engineering.

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In vitro characterization of polyesters of aconitic acid, glycerol, and cinnamic acid for bone tissue engineering

Journal of Biomaterials Applications ◽

10.1177/0885328214553961 ◽

2014 ◽

Vol 29 (8) ◽

pp. 1075-1085 ◽

Cited By ~ 2

Author(s):

Akanksha Kanitkar ◽

Cong Chen ◽

Mollie Smoak ◽

Katie Hogan ◽

Thomas Scherr ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Cinnamic Acid ◽

In Vitro Characterization ◽

Aconitic Acid

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Synthesis and Characterization of Silver-Coated Polymeric Scaffolds for Bone Tissue Engineering: Antibacterial and In Vitro Evaluation of Cytotoxicity and Biocompatibility

ACS Omega ◽

10.1021/acsomega.0c05596 ◽

2021 ◽

Vol 6 (6) ◽

pp. 4335-4346

Author(s):

Muhammad Umar Aslam Khan ◽

Saiful Izwan Abd Razak ◽

Hassan Mehboob ◽

Mohammed Rafiq Abdul Kadir ◽

T. Joseph Sahaya Anand ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Synthesis And Characterization ◽

In Vitro Evaluation ◽

Polymeric Scaffolds

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Electro-conductive carbon nanofibers containing ferrous sulfate for bone tissue engineering

10.1101/2020.12.27.424517 ◽

2020 ◽

Author(s):

Houra Nekounam ◽

Hadi Samadian ◽

Fatemeh Asghari ◽

Reza Faridi Majidi

Keyword(s):

Heat Treatment ◽

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Carbon Nanofibers ◽

Electrical Resistance ◽

Ferrous Sulfate ◽

Electrical Resistance Measurement ◽

O 15

AbstractThe application of electroactive scaffolds can be promising for bone tissue engineering applications. In the current paper, we aimed to fabricate an electro-conductive scaffold based on carbon nanofibers (CNFs) containing ferrous sulfate. FeSO4·7H2O salt with different concentrations 5, 10, and 15 wt%, were blended with polyacrylonitrile (PAN) polymer as the precursor and converted to Fe2O3/CNFs nanocomposite by electrospinning and heat treatment. The characterization was conducted using SEM, EDX, XRD, FTIR, and Raman methods. The results showed that the incorporation of Fe salt did not induce an adverse effect on the nanofibers’ morphology. EDX analysis confirmed that the Fe are uniformly dispersed throughout the CNF mat. FTIR spectroscopy showed the interaction of Fe salt with PAN polymer. Raman spectroscopy showed that the incorporation of FeSO4·7H2O reduced the ID/IG ratio, indicating more ordered carbon in the synthesized nanocomposite. Electrical resistance measurement depicted that, although the incorporation of ferrous sulfate reduced the electrical conductivity, the conductive is suitable for electrical stimulation. The in vitro studies revealed that the prepared nanocomposites were cytocompatible and only negligible toxicity (less than 10%) induced by CNFs/Fe2O3 fabricated from PAN FeSO4·7H2O 15%. These results showed that the fabricated nanocomposites could be applied as the bone tissue engineering scaffold.

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Development of functionalized multi-walled carbon nanotube-based polysaccharide–hydroxyapatite scaffolds for bone tissue engineering

RSC Advances ◽

10.1039/c6ra16709h ◽

2016 ◽

Vol 6 (85) ◽

pp. 82385-82393 ◽

Cited By ~ 13

Author(s):

R. Rajesh ◽

Y. Dominic Ravichandran ◽

M. Jeevan Kumar Reddy ◽

Sung Hun Ryu ◽

A. M. Shanmugharaj

Keyword(s):

Tissue Engineering ◽

Cell Proliferation ◽

Carbon Nanotube ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Gellan Gum ◽

Alp Activity ◽

Multi Walled Carbon Nanotube ◽

First Time

fMWCNT–amylopectin–HAP and fMWCNT–gellan gum–HAP were prepared and characterized and their in vitro cell proliferation and ALP activity were checked for the first time.

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