Extracts of Peppermint, Chamomile and Lavender as Antioxidants

2018 ◽  
Vol 762 ◽  
pp. 31-35 ◽  
Author(s):  
Inese Mieriņa ◽  
Laura Jakaite ◽  
Sabine Kristone ◽  
Laura Adere ◽  
Mara Jure

This paper is devoted to the studies of total polyphenol content and antiradical activity of peppermint, chamomile and lavender grown in local backyard gardens of Latvia. Different solvents (96, 70 and 40% ethanol) and extraction methods (maceration at room temperature at dark or under day-light irradiation, as well as reflux) were tested. All extracts were analyzed for total polyphenol content (Folin–Ciocalteu method), as well as for antiradical activity (DPPH and GO assays). It was found out that local peppermint and chamomile is as good source of polyphenols as those plants grown in southern countries; however, the total polyphenol content of lavender is lower than that mentioned in literature. The highest polyphenol content for all plant materials was achieved, when 70% ethanol was used for extraction. Peppermint extracts were found as most powerful free radical scavengers among all tested plant materials. 96% Ethanol extract of peppermint and chamomile demonstrated the highest antiradical activity, while 70% ethanol was the most suitable for preparation of lavender extract with optimal free radical scavenging activity.

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 305 ◽  
Author(s):  
Manel Ouerfelli ◽  
Juliana Villasante ◽  
Leila Bettaieb Ben Kaâb ◽  
MaríaPilar Almajano

The aim of this study was to determine the total polyphenol content, radical scavenging and antimicrobial activities of Azadirachta indica (A. indica) and to evaluate their effect on shelf-life stability of raw beef patties during refrigerated storage at 4 ± 1 °C. During 11 days of storage, the antioxidant effect of A. indica on ground beef meat was investigated by the determination of lipid oxidation, pH, anti-radical activity, color, hexanal content, and microbial growth. The results obtained showed that fresh A. indica leaves and synthetic conservative behaved in the same way and retarded the lipid oxidation of chilled beef patties while increasing their pH (5.40 and 5.45, respectively). It can also be said that A. indica limited the loss of color, reduced the metmyoglobin formation (36.70%) and had a significant effect on bacterial growth and hexanal content. In addition, the results obtained through anti-radical and antimicrobial properties showed proportional values of total polyphenol content and radical scavenging activity of leaf extracts as they showed their antimicrobial effect against some bacteria such as Staphylococcus aureus and Micrococcus luteus, among others. These results support the involvement of A. indica in the food industry as a natural antioxidant that could replace synthetic ones.


2020 ◽  
Vol 38 (No. 5) ◽  
pp. 287-292
Author(s):  
Leila Arfaoui

Both yogurt and dates are functional foods known for their valuable nutrients and health benefits. Therefore, this study was conducted to produce two types of date-enriched yogurts (20% wt/wt) to enhance their nutritive value and health benefits. While yogurt A was made with dates blended with milk, yogurt B was produced using small pieces of dates that were added to milk before fermentation. Both date-enriched yogurts were assessed for their physicochemical composition, total polyphenol content (TPC) and radical scavenging activity (RSA). The results showed that the addition of dates significantly enhanced carbohydrate, mineral and total solids contents of yogurts A and B (P < 0.05). Furthermore, dates significantly enhanced the TPC (34 and 37 mg GAE 100 g–1 for yogurt A and B, respectively) and the RSA (51% for yogurt A versus 57% for yogurt B) of date-enriched yogurts especially when dates were added as small pieces. During cold storage, both TPC and RSA decreased in all yogurt samples; however, they were maintained higher in date-enriched yogurts.


2013 ◽  
Vol 32 (1) ◽  
pp. 227 ◽  
Author(s):  
Andreja Leskovac ◽  
Gordana Joksic ◽  
Igor Pasti ◽  
Tamara Lazarevic-Pasti ◽  
Branislav Nastasijevic ◽  
...  

Herbal preparation Chlamyfin was investigated for its total polyphenol content, 2,2-diphenyl-1- picrylhydrazyl (DPPH) scavenging activity, and anti-inflammatory and genotoxic properties. A high total polyphenol content provided evidence of high DPPH radical scavenging activity (IC50 = 4.96 ± 0.23 μg/ml). Analysis of the electrochemical behavior of Chlamyfin indicated high reducing ability, i.e., high antioxidant capacity, in agreement with the DPPH test. Analysis of myeloperoxidase (MPO) inhibition by Chlamyfin suggested anti-inflammatory action (IC50 values of 5.40 μg/ml and 4.45 μg/ml for an incubation time of 10 and 30 min, respectively). For genotoxic assessment, oxidative stress was induced by irradiation of peripheral whole blood with gamma-radiation in vitro. In the presence of Chlamyfin, reduced incidence of micronuclei without disturbance to the proliferative potential of cells was evidenced in both irradiated and unirradiated samples, indicating its genoprotective properties. It was shown that Chlamyfin, in addition to its bactericidal effect, also possesses strong antioxidant, anti-inflammatory and anti-genotoxic properties.


2021 ◽  
pp. 10-12
Author(s):  
S Srinivasan ◽  
A Rayar

Introduction:Medicinal plants have been used for the treatment of diseases all over the world before the advent of articial clinical drugs and are known to contain substances that can be used for therapeutic uses. Antioxidations act asreducing agents, free radical scavengers, quenchers of singlet oxygen molecule, and activators for antioxidative enzyme to reduce the damage caused by free radicals in biological pattern. Materials and Methods:The isolated organic compound from Mimosa nilotica was subjected to column chromatographic separation and was characterized 1 13 by UV, IR, H-NMR and C-NMR.DPPH assay was conducted to assess the effect of the ethanolic extract and 7-hydroxy–4-methylcoumarin. Result and Discussion:The reports of the in-vitro antioxidation assay indicated that the ethanol extract was more potent than 7-hydroxy–4- methylcoumarin. 7-hydroxy–4-methylcoumarin and ethanolic extractexpress excellent free radical scavenging activity. From the reports it is proved that the reducing power of bioactive constituent is associated with antioxidationpotential. Thus a correlation is proved between reducing power and the antioxidation effect. Conclusion:Previous reports are conrmed that the reducing power of bioactive constituents is associated with antioxidation potential. People who eat various parts of fruits and herbs have a lower risk of heart disease and some neurological diseases and there is evidence that some types of herbs, and fruits in general, protect against some malignant.


2003 ◽  
Vol 9 (6) ◽  
pp. 383-387 ◽  
Author(s):  
P. Kefalas ◽  
S. Kallithraka ◽  
I. Parejo ◽  
D. P. Makris

The antiradical and hydroxyl free radical scavenging activities were estimated in twenty-five, aged red wines from different areas in Greece. The antiradical activity (AAR) was determined by means of the wellknown DPPH• method, and its values ranged from 24.7 to 125.1. A novel, chemiluminescence-based, highly sensitive assay was applied for determination of the hydroxyl free radical scavenging activity (SAHFR), which varied from 1.62 to 12.22 mM quercetin equivalents. The values from the two assays correlated very well (r2 =0.8542, P<0.001), which confirmed an important relationship between SAHFR and AAR. This tendency in aged red wines, which may be significant in evaluating the antioxidant behaviour of red wine polyphenols, is discussed on the basis of previous research and relevant data.


Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2557
Author(s):  
Sorin Ciulca ◽  
Gheorghe Roma ◽  
Ersilia Alexa ◽  
Isidora Radulov ◽  
Ileana Cocan ◽  
...  

Fruits of bilberry (Vaccinium myrtillus L.) are valued mainly for their nutraceutical properties, and are among the fruits with the highest antioxidant activity due to their high content of phenolic compounds. The aim of this research was to assess the total polyphenol content and antioxidant activity of fruits in six wild bilberry populations from two regions of Romania over three years. The total polyphenol content was determined according to the Folin–Ciocalteu modified method, while the antioxidant activity was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay. The Padis and Raul Lung populations registered the highest values of polyphenol content and antioxidant activity, as such the fruits of these bilberry populations could be considered potential sources of antioxidants for direct consumption or for use as ingredients for food products or food supplements. Significant variation of total polyphenol content and antioxidant activity was observed both between populations from the same region and from different regions. The low level of broad sense heritability for total polyphenol content and antioxidant activity associated with the high effects of year, and population–year interaction indicates that the accumulation of polyphenols in bilberry fruits is influenced by changes in environmental conditions.


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