Growth of Acidithiobacillus thiooxidans Biofilm on Glass, Concrete and Stoneware

2015 ◽  
Vol 227 ◽  
pp. 286-289 ◽  
Author(s):  
Weronika Dec ◽  
Beata Cwalina ◽  
Joanna Michalska ◽  
Daria Merkuda
Keyword(s):  
Incubation Time ◽  
Biofilm Formation ◽  
Essential Role ◽  
Acidithiobacillus Thiooxidans ◽  
Sulfur Oxidizing ◽  
Microbiologically Induced Corrosion ◽  
Comparable Intensity ◽  
Sulfur Oxidizing Bacteria

Biofilms formed by various microorganisms are often responsible for microbiologically induced corrosion of materials exposed to moisture. Many bacteria strains may form biofilms on different mineral materials including concrete and stoneware. Among them, the sulfur-oxidizing bacteria of Acidithiobacillus genus, especially of A. thiooxidans species play an essential role in these materials' destruction. In the present study we observed comparable intensity of A. thiooxidans growth on concrete and glass, and slower biofilm formation on stoneware. Prolongation of incubation time caused an increase in biomass of A. thiooxidans biofilm formed on glass and stoneware. Amongst the investigated mineral materials, the concrete proved to be the most susceptible to deterioration by A. thiooxidans bacteria.

scholarly journals Novel Approaches for Biocorrosion Mitigation in Sewer Systems

Chemistry ◽  
2021 ◽  
Vol 3 (4) ◽  
pp. 1166-1177
Author(s):  
Georgios Fytianos ◽  
Dimitra Banti ◽  
Esmeralda Dushku ◽  
Efthimios Papastergiadis ◽  
Minas Yiangou ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Tio2 Nanoparticles ◽  
Acidithiobacillus Thiooxidans ◽  
Sewer Pipes ◽  
Sewer Systems ◽  
Sulfur Oxidizing ◽  
Novel Approaches ◽  
Potential Inhibitors ◽  
Microbiologically Induced Corrosion ◽  
Sulfur Oxidizing Bacteria

Concrete sewer pipes can be corroded by the biogenic sulfuric acid (H2SO4) generated from microbiological activities in a process called biocorrosion or microbiologically induced corrosion (MIC). In this study, inhibitors that can reduce Acidithiobacillus thiooxidans growth and thus may reduce the accumulation of biofilm components responsible for the biodegradation of concrete were used. D-tyrosine, tetrakis hydroxymethyl phosphonium sulfate (THPS) and TiO2 nanoparticles were investigated as potential inhibitors of sulfur-oxidizing bacteria (SOB) growth. Results showed that most of the chemicals used can inhibit SOB growth at a concentration lower than 100 mg/L. TiO2 nanoparticles exhibited the highest biocide effect and potential biocorrosion mitigation activity, followed by D-tyrosine and THPS.

Revealing biogenic sulfuric acid corrosion in sludge digesters: detection of sulfur-oxidizing bacteria within full-scale digesters

2014 ◽  
Vol 70 (8) ◽  
pp. 1405-1411 ◽  
Author(s):  
B. Huber ◽  
J. E. Drewes ◽  
K. C. Lin ◽  
R. König ◽  
E. Müller
Keyword(s):  
Sulfuric Acid ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Acid Corrosion ◽  
Full Scale ◽  
Acidithiobacillus Thiooxidans ◽  
Thiobacillus Thioparus ◽  
Gradient Gel Electrophoresis ◽  
Sulfuric Acid Corrosion ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

Biogenic sulfuric acid corrosion (BSA) is a costly problem affecting both sewerage infrastructure and sludge handling facilities such as digesters. The aim of this study was to verify BSA in full-scale digesters by identifying the microorganisms involved in the concrete corrosion process, that is, sulfate-reducing (SRB) and sulfur-oxidizing bacteria (SOB). To investigate the SRB and SOB communities, digester sludge and biofilm samples were collected. SRB diversity within digester sludge was studied by applying polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) targeting the dsrB-gene (dissimilatory sulfite reductase beta subunit). To reveal SOB diversity, cultivation dependent and independent techniques were applied. The SRB diversity studies revealed different uncultured SRB, confirming SRB activity and H2S production. Comparable DGGE profiles were obtained from the different sludges, demonstrating the presence of similar SRB species. By cultivation, three pure SOB strains from the digester headspace were obtained including Acidithiobacillus thiooxidans, Thiomonas intermedia and Thiomonas perometabolis. These organisms were also detected with PCR-DGGE in addition to two new SOB: Thiobacillus thioparus and Paracoccus solventivorans. The SRB and SOB responsible for BSA were identified within five different digesters, demonstrating that BSA is a problem occurring not only in sewer systems but also in sludge digesters. In addition, the presence of different SOB species was successfully associated with the progression of microbial corrosion.

Bioleaching of a Marine Hydrothermal Sulfide Ore with Mesophiles, Moderate Thermophiles and Thermophiles

2013 ◽  
Vol 825 ◽  
pp. 229-232
Author(s):  
Hananeh Korehi ◽  
Axel Schippers
Keyword(s):  
Acidithiobacillus Thiooxidans ◽  
Iron Extraction ◽  
Valuable Metals ◽  
Acidianus Brierleyi ◽  
Moderate Thermophiles ◽  
Shake Flasks ◽  
The Indian Ocean ◽  
Sulfur Oxidizing ◽  
A Site ◽  
Sulfur Oxidizing Bacteria

Marine hydrothermal polymetallic sulfide ores contain high amounts of valuable metals such as Cu, Pb, Zn, Au, Ag, as well as In, Ge, Bi, and Se. Samples from a site in the Indian Ocean were taken during a BGR ship cruise, crushed and sieved for bioleaching experiments to reveal the extraction of the various metals. Chalcopyrite was the main mineral, the total copper content was 38.5 %wt. Comparative bioleaching with mesophilic, moderate thermophilic and thermophilic acidophilic iron- and sulfur-oxidizing bacteria and archaea was investigated. Batch culture experiments were conducted at 2% (w/v) pulp density in shake flasks in the presence of Acidithiobacillus ferrooxidans, Acidiphilium sp. and Acidithiobacillus thiooxidans as mesophiles (30°C), a mixed culture of moderate thermopilic iron- and sulfur oxidizing bacteria (50°C) and the thermophile Acidianus brierleyi (70°C). The results after four weeks showed most effective dissolution of copper in the presence of A. brierleyi (up to 4.3 g/l), compared with moderate thermophiles and mesophiles (3.3 g/l and 2.5 g/l, respectively). Furthermore, the bioleaching performance was approved with dissolved iron concentrations. Conclusively, an increase in temperature from 30 °C to 70 °C had a major impact on bioleaching efficiency. Copper and iron extraction efficiency occurred in the order thermophiles, moderate thermophiles, mesophiles.

scholarly journals Succession of Sulfur-Oxidizing Bacteria in the Microbial Community on Corroding Concrete in Sewer Systems

2006 ◽  
Vol 73 (3) ◽  
pp. 971-980 ◽  
Author(s):  
Satoshi Okabe ◽  
Mitsunori Odagiri ◽  
Tsukasa Ito ◽  
Hisashi Satoh
Keyword(s):  
Sulfuric Acid ◽  
Microbial Community ◽  
Concrete Surface ◽  
Molecular Techniques ◽  
Rrna Gene ◽  
Acidithiobacillus Thiooxidans ◽  
Community Members ◽  
Sewer Systems ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

ABSTRACT Microbially induced concrete corrosion (MICC) in sewer systems has been a serious problem for a long time. A better understanding of the succession of microbial community members responsible for the production of sulfuric acid is essential for the efficient control of MICC. In this study, the succession of sulfur-oxidizing bacteria (SOB) in the bacterial community on corroding concrete in a sewer system in situ was investigated over 1 year by culture-independent 16S rRNA gene-based molecular techniques. Results revealed that at least six phylotypes of SOB species were involved in the MICC process, and the predominant SOB species shifted in the following order: Thiothrix sp., Thiobacillus plumbophilus, Thiomonas intermedia, Halothiobacillus neapolitanus, Acidiphilium acidophilum, and Acidithiobacillus thiooxidans. A. thiooxidans, a hyperacidophilic SOB, was the most dominant (accounting for 70% of EUB338-mixed probe-hybridized cells) in the heavily corroded concrete after 1 year. This succession of SOB species could be dependent on the pH of the concrete surface as well as on trophic properties (e.g., autotrophic or mixotrophic) and on the ability of the SOB to utilize different sulfur compounds (e.g., H2S, S0, and S2O3 2−). In addition, diverse heterotrophic bacterial species (e.g., halo-tolerant, neutrophilic, and acidophilic bacteria) were associated with these SOB. The microbial succession of these microorganisms was involved in the colonization of the concrete and the production of sulfuric acid. Furthermore, the vertical distribution of microbial community members revealed that A. thiooxidans was the most dominant throughout the heavily corroded concrete (gypsum) layer and that A. thiooxidans was most abundant at the highest surface (1.5-mm) layer and decreased logarithmically with depth because of oxygen and H2S transport limitations. This suggested that the production of sulfuric acid by A. thiooxidans occurred mainly on the concrete surface and the sulfuric acid produced penetrated through the corroded concrete layer and reacted with the sound concrete below.

scholarly journals Biofilm Formation Is Crucial for Efficient Copper Bioleaching From Bornite Under Mesophilic Conditions: Unveiling the Lifestyle and Catalytic Role of Sulfur-Oxidizing Bacteria

2021 ◽  
Vol 12 ◽  
Author(s):  
Roberto A. Bobadilla-Fazzini ◽  
Ignacio Poblete-Castro
Keyword(s):  
Biofilm Formation ◽  
Mining Industry ◽  
Copper Recovery ◽  
Acidithiobacillus Thiooxidans ◽  
Cu Content ◽  
Planktonic Form ◽  
A Cell ◽  
Catalytic Role ◽  
Sulfur Oxidizing

Biofilm formation within the process of bioleaching of copper sulfides is a relevant aspect of iron- and sulfur-oxidizing acidophilic microorganisms as it represents their lifestyle in the actual heap/dump mining industry. Here, we used biofilm flow cell chambers to establish laminar regimes and compare them with turbulent conditions to evaluate biofilm formation and mineralogic dynamics through QEMSCAN and SEM-EDS during bioleaching of primary copper sulfide minerals at 30°C. We found that laminar regimes triggered the buildup of biofilm using Leptospirillum spp. and Acidithiobacillus thiooxidans (inoculation ratio 3:1) at a cell concentration of 106 cells/g mineral on bornite (Cu5FeS4) but not for chalcopyrite (CuFeS2). Conversely, biofilm did not occur on any of the tested minerals under turbulent conditions. Inoculating the bacterial community with ferric iron (Fe3+) under shaking conditions resulted in rapid copper recovery from bornite, leaching 40% of the Cu content after 10 days of cultivation. The addition of ferrous iron (Fe2+) instead promoted Cu recovery of 30% at day 48, clearly delaying the leaching process. More efficiently, the biofilm-forming laminar regime almost doubled the leached copper amount (54%) after 32 days. In-depth inspection of the microbiologic dynamics showed that bacteria developing biofilm on the surface of bornite corresponded mainly to At. Thiooxidans, while Leptospirillum spp. were detected in planktonic form, highlighting the role of biofilm buildup as a means for the bioleaching of primary sulfides. We finally propose a mechanism for bornite bioleaching during biofilm formation where sulfur regeneration to sulfuric acid by the sulfur-oxidizing microorganisms is crucial to prevent iron precipitation for efficient copper recovery.

Effects of activated carbon on treatment of photo-processing waste by sulfur-oxidizing bacteria

1997 ◽  
Vol 35 (7) ◽  
pp. 187-195 ◽  
Author(s):  
Binle Lin ◽  
K. Futono ◽  
A. Yokoi ◽  
M. Hosomi ◽  
A. Murakami
Keyword(s):  
Activated Carbon ◽  
Treatment Effectiveness ◽  
Environmental Concern ◽  
Type Systems ◽  
Continuous Treatment ◽  
Processing Waste ◽  
Treatment Technology ◽  
Adsorption Effect ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

Establishing economic treatment technology for safe disposal of photo-processing waste (PW) has most recently become an urgent environmental concern. This paper describes a new biological treatment process for PW using sulfur-oxidizing bacteria (SOB) in conjunction with activated carbon (AC). Batch-type acclimation and adsorption experiments using SOB/PAC, SOB/PNAC, and SOB reactor type systems demonstrated that AC effectively adsorbs the toxic/refractory compounds which inhibit thiosulfate oxidization of SOB in PW. Thus, to further clarify the effect of AC, we performed a long-term (≈ 160 d) continuous-treatment experiment on 4- to 8-times dilution of PW using a SOB/GAC system which simulated a typical wastewater treatment system based on an aerobic activated sludge process that primarily uses acclimated SOB. The thiosulfate load and hydraulic retention time (HRT) were fixed during treatment such that they ranged from 0.8-3.7 kg S2O32-/l/d and 7.7-1.9 d, respectively. As expected, continuous treatment led to breakthrough of the adsorption effect of GAC. Renewing the GAC and continuing treatment for about 10 d demonstrated good treatment effectiveness.

scholarly journals Sulfur bacteria promote dissolution of authigenic carbonates at marine methane seeps

2021 ◽  
Author(s):  
Dalton J. Leprich ◽  
Beverly E. Flood ◽  
Peter R. Schroedl ◽  
Elizabeth Ricci ◽  
Jeffery J. Marlow ◽  
...  
Keyword(s):  
Carbonate Rocks ◽  
Sulfur Oxidation ◽  
Methane Seeps ◽  
Etch Pits ◽  
Active Dissolution ◽  
Authigenic Carbonates ◽  
Sulfur Bacteria ◽  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria

AbstractCarbonate rocks at marine methane seeps are commonly colonized by sulfur-oxidizing bacteria that co-occur with etch pits that suggest active dissolution. We show that sulfur-oxidizing bacteria are abundant on the surface of an exemplar seep carbonate collected from Del Mar East Methane Seep Field, USA. We then used bioreactors containing aragonite mineral coupons that simulate certain seep conditions to investigate plausible in situ rates of carbonate dissolution associated with sulfur-oxidizing bacteria. Bioreactors inoculated with a sulfur-oxidizing bacterial strain, Celeribacter baekdonensis LH4, growing on aragonite coupons induced dissolution rates in sulfidic, heterotrophic, and abiotic conditions of 1773.97 (±324.35), 152.81 (±123.27), and 272.99 (±249.96) μmol CaCO3 • cm−2 • yr−1, respectively. Steep gradients in pH were also measured within carbonate-attached biofilms using pH-sensitive fluorophores. Together, these results show that the production of acidic microenvironments in biofilms of sulfur-oxidizing bacteria are capable of dissolving carbonate rocks, even under well-buffered marine conditions. Our results support the hypothesis that authigenic carbonate rock dissolution driven by lithotrophic sulfur-oxidation constitutes a previously unknown carbon flux from the rock reservoir to the ocean and atmosphere.

Extracting copper and cobalt from non-ferrous residues by iron- and sulfur-oxidizing bacteria

2021 ◽  
Author(s):  
Jianxing Sun ◽  
Wenxian Liu ◽  
Ruichang Tang ◽  
Haina Cheng ◽  
Ronghui Liu ◽  
...  
Keyword(s):  
Sulfur Oxidizing ◽  
Sulfur Oxidizing Bacteria
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