Characterization and In Vitro Toxicity of French Process Zinc Oxide Nanoparticles with High Surficial Zinc

2019 ◽  
Vol 290 ◽  
pp. 274-279
Author(s):  
Sufiniza Nordin ◽  
Shahrom Mahmud ◽  
Azman Seeni ◽  
Nur Mariam Kamaruddin ◽  
Nur Syuhada Ahmad
Keyword(s):  
L929 Cell ◽  
Average Crystallite Size ◽  
Fibroblast Cell ◽  
In Vitro Toxicity ◽  
Visible Absorption ◽  
Zno Nanopowder ◽  
L929 Fibroblast ◽  
Calculated Average ◽  
L929 Cell Line

In this study, we investigated in vitro toxicity of ZnO nanopowder on L929 fibroblast cell lines. The ZnO nanoparticles were observed to possess relatively more surficial zinc compared to oxygen. Field-emission scanning electron microscope (FESEM) data revealed that the particle morphologies consisted of nanorods, platelets and nodules between 40-100 nm size range. EDS confirmed that there were more zinc elements on the surfaces of the particles. XRD results showed that the calculated average crystallite size of ZnO nanopowder was 44.28 nm. The optical band gap calculated was 3.298 eV based on UV-visible absorption spectra. In vitro toxicity results showed that ZnO concentration at 0.3125mM, 0.625mM and 1.25 mM were considered non-toxic to L929 cell line since the cell viability was higher than 70 % after 72 hours treatment whereas the ZnO nanopowder concentration above 2.5mM was considered toxic. High surficial zinc atoms on ZnO particles could have been a significant factor in cell toxicity.

scholarly journals Development of Antimicrobial Conjugates and Evaluating its Antibacterial potential and Wound Healing ability

2021 ◽  
Vol 23 (09) ◽  
pp. 540-555
Author(s):  
Deepak Tom Jose ◽  
◽  
Sivagurunathan, P ◽  
Aswini, B ◽  
Dinesh, MD ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
L929 Fibroblast ◽  
Microscopic Studies ◽  
Engineered Materials ◽  
Cell Migration And Proliferation ◽  
Antibacterial Potential

Antimicrobial peptides from Streptomyces sp. and marine fish (Carangoides malabaricus) were extracted and developed as conjugates in the present study. The objective was framed to analyze the ability of conjugate to retard the growth of test bacteria causing diabetic foot ulcers. Fibroblast cell adhesion on AMP conjugates coated mesh samples were recorded using microscopic studies with an aim of developing a novel tissue engineered wound dressing material. Thus developed tissue engineered materials were evaluated for its antibacterial potential against wound pathogens; and to assay the wound healing ability using a standard in vitro wound scratch method. Tissue engineered materials were developed using L929 fibroblast cells. L929 fibroblast cells attachment and its stage wise development on wound dressing mesh materials were microscopically observed. In vitro wound healing assay revealed that the developed conjugates (containing AMPs) exhibited cell migration and proliferation after 12th hour of incubation indicating the wound healing abilities. The results showed that the developed tissue engineered wound dressing material has commercial interest in near future.

Cytotoxicity of Pharma Grade ZnO with Higher Surficial Oxygen on L929 Mouse Cell

2019 ◽  
Vol 290 ◽  
pp. 286-291
Author(s):  
Nur Mariam Kamaruddin ◽  
Shahrom Mahmud ◽  
Sufiniza Nordin ◽  
Abdulsalam Abuelsamen ◽  
Azman Seeni ◽  
...  
Keyword(s):  
Cell Viability ◽  
Particle Surface ◽  
L929 Cell ◽  
Average Crystallite Size ◽  
Fibroblast Cell ◽  
Mouse Cell ◽  
Mouse Fibroblast ◽  
Cytotoxicity Test ◽  
Zno Powder ◽  
L929 Mouse

Apart from being a promising optoelectronic devices such as photodetector and sensors, ZnO has many dental and biomedical applications. ZnO has been known to possess strong toxicity towards bacteria, cancer and fungi. Cytotoxicity test of pharmaceutical grade of ZnO on L929 mouse fibroblast cell lines was carried out using trypan blue assay. ZnO was characterized for its morphology, structure and optical properties using FESEM, EDS, UV-Vis and XRD. ZnO exhibited various morphologies like rod, platelet, slab and irregular-shaped particles. EDS data showed the ZnO powder possessed relatively higher oxygen atomic percentage if compared to zinc atoms with an oxygen-to-zinc ratio of 1.219. The average crystallite size obtained was about 39 nm. The percentage of cell viability on L929 cell was decreased with increasing ZnO concentrations. The cells viability after 72h were achieved and the concentration of ZnO below 1 mM was summarized as non-toxic after treated with ZnO. The higher surficial oxygen on ZnO particle surface could have promoted higher generation of reactive oxygen species that caused lower L929 cell viability.

scholarly journals In vitro toxicity and bioimaging studies of gold nanorods formulations coated with biofunctional thiol-PEG molecules and Pluronic block copolymers

2014 ◽  
Vol 5 ◽  
pp. 546-553 ◽  
Author(s):  
Tianxun Gong ◽  
Douglas Goh ◽  
Malini Olivo ◽  
Ken-Tye Yong
Keyword(s):  
Gold Nanorods ◽  
Colloidal Stability ◽  
Dark Field ◽  
Solution Phase ◽  
In Vitro Toxicity ◽  
Visible Absorption ◽  
Aspect Ratios ◽  
Biological Studies

In this work, we investigated the cytotoxicity, colloidal stability and optical property of gold nanorods before and after functionalizing them with thiolated PEG and Pluronic triblock copolymer (PEO–PPO–PEO) molecules. The morphology of functionalized gold nanorods was characterized by UV–visible absorption spectroscopy, transmission electron microscopy, and dynamic light scattering. Solution phase synthesis of gold nanorods has remained the method of choice for obtaining varying shapes and aspect ratios of rod nanoparticles. This method typically involves the use of cetyltrimethylammonium bromide (CTAB) surfactants as directing agents to grow gold nanorods in the solution phase. The as-synthesized gold nanorods surfaces are terminated with CTAB molecules and this formulation gives rise to adverse toxicity in vitro and in vivo. To employ the gold nanorods for biological studies, it is important to eliminate or minimize the exposure of CTAB molecules from the gold nanorods surface to the local environment such as cells or tissues. Complete removal of CTAB molecules from the gold nanorods surface is unfeasible as this will render the gold nanorods structurally unstable, causing the aggregation of particles. Here, we investigate the individual use of thiolated PEG and PEO–PPO–PEO as capping agents to reduce the cytotoxicity of gold nanorods formulation, while maintaining the optical, colloidal, and structural properties of gold nanorods. We found that encapsulating gold nanorods with the thiolated PEG or PEO–PPO–PEO molecules guarantees the stability and biocompatibility of the nanoformulation. However, excessive use of these molecules during the passivation process leads to a reduction in the overall cell viability. We also demonstrate the use of the functionalized gold nanorods as scattering probes for dark-field imaging of cancer cells thereby demonstrating their biocompatibility. Our results offer a unique solution for the future development of safe scattering color probes for clinical applications such as the long term imaging of cells and tissues.

In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line

Chemosphere ◽  
2017 ◽  
Vol 173 ◽  
pp. 452-459 ◽  
Author(s):  
Teuta Eljezi ◽  
Pierre Pinta ◽  
Damien Richard ◽  
Jérémy Pinguet ◽  
Jean-Michel Chezal ◽  
...  
Keyword(s):  
Cell Line ◽  
L929 Cell ◽  
Cytotoxic Effects ◽  
Primary Metabolites ◽  
L929 Cell Line

scholarly journals Assessment of cytotoxic and antimicrobial activity of selected gingival haemostatic agents – in vitro study

2020 ◽  
Vol 22 (3) ◽  
Author(s):  
Maria Szymonowicz ◽  
_ Agnieszka ◽  
Magdalena Pajączkowska ◽  
Joanna Nowicka ◽  
Kamila Wiśniewska ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cell Line ◽  
Mtt Assay ◽  
In Vitro Study ◽  
L929 Cell ◽  
Aluminium Chloride ◽  
Srb Assay ◽  
Haemostatic Agents ◽  
L929 Cell Line

Purpose: The present research aimed to determine whether and how the aluminium chloride – based materials affect the cell line of the bacterial line and fungi. Methods: Cytotoxicity of haemostatic astringents: Alustat (liquid), Alustat (gel), Alustat (foam), Alustin, Hemostat, Racestyptine and Traxodent containing AlCl3 was conducted on L929 cell line with the use of MTT and SRB assays. The antimicrobial activity (CFU and MIC) against C. albicans, S. mutans, L. rhamnosus was determined. Results: In the MTT results, cell viability for all agents were very low. In SRB, the lowest cytotoxicity was demonstrated for Hemostat and Alustat (foam), Traxodent and Racestyptine. Total reduction of the CFU of S. mutans was observed. Alustat (gel) and Alustat (liquid) completely inhibited the growth of C. albicans, S. mutans and L. rhamnosus. Conclusions: The viability of L929 cells obtained in the SRB assay is more reliable than that obtained in the MTT assay, in the case of gingival haemostatic agents.

scholarly journals Development of Antimicrobial Conjugates and Evaluating its Antibacterial potential and Wound Healing ability

2021 ◽  
Vol 23 (10) ◽  
pp. 206-221
Author(s):  
Deepak Tom Jose ◽  
◽  
Sivagurunathan, P ◽  
Aswini, B, ◽  
Uma, C ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Fibroblast Cell ◽  
Fibroblast Cells ◽  
L929 Fibroblast ◽  
Microscopic Studies ◽  
Engineered Materials ◽  
Cell Migration And Proliferation ◽  
Antibacterial Potential

Antimicrobial peptides from Streptomyces sp. and marine fish (Carangoides malabaricus) were extracted and developed as conjugates in the present study. The objective was framed to analyze the ability of conjugate to retard the growth of test bacteria causing diabetic foot ulcers. Fibroblast cell adhesion on AMP conjugates coated mesh samples were recorded using microscopic studies with an aim of developing a novel tissue engineered wound dressing material. Thus developed tissue engineered materials were evaluated for its antibacterial potential against wound pathogens; and to assay the wound healing ability using a standard in vitro wound scratch method. Tissue engineered materials were developed using L929 fibroblast cells. L929 fibroblast cells attachment and its stage wise development on wound dressing mesh materials were microscopically observed. In vitro wound healing assay revealed that the developed conjugates (containing AMPs) exhibited cell migration and proliferation after 12th hour of incubation indicating the wound healing abilities. The results showed that the developed tissue engineered wound dressing material has commercial interest in near future.

scholarly journals Cytotoxicity of endodontic irrigants containing calcium hydroxide and sodium lauryl sulphate on fibroblasts derived from mouse L929 cell line

2009 ◽  
Vol 20 (2) ◽  
pp. 118-121 ◽  
Author(s):  
Sérgio Valmor Barbosa ◽  
Cristiane Maria Sodré Barroso ◽  
Patrícia Alvarez Ruiz
Keyword(s):  
Cell Line ◽  
Calcium Hydroxide ◽  
L929 Cell ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Sodium Lauryl Sulphate ◽  
Control Group ◽  
Fibroblast Cell Line ◽  
Significance Level ◽  
L929 Cell Line

The aim of this study was to evaluate the cytotoxicity of root canal irrigating solutions containing calcium hydroxide and sodium lauryl sulphate on fibroblasts derived from L929 cell line. Saturated calcium hydroxide aqueous solution (CH), sodium lauryl sulphate (SLS) and SLS associated with calcium hydroxide (HCT20) were diluted with sterile distilled water at 50%, 20%, 10% and 5% concentrations. Minimum essential medium (MEM) served as the control group. The cytotoxicity of the solutions was evaluated on L929 mouse fibroblast cell line, at 4 and 24 h of contact time by the 51Cr radiotracer method. Data were compared and statistical inferences were made with the chi-square test. In all analysis, significance level was set at 5%. CH and HCT20 showed toxicity at 50% concentration, while at concentrations lower than 50% these solutions showed cell tolerance. SLS was cytotoxic at all concentrations. In conclusion, the association of calcium hydroxide and SLS (HCT20) combines the beneficial properties of these solutions and was not harmful to the fibroblast cell line, seeming to be a suitable endodontic irrigating solution.

IN VITRO CYTOCOMPATIBILITY OF SYNTHETIC CALCIUM PHOSPHATE POWDER ON L929 FIBROBLAST CELL

2015 ◽  
Vol 23 (1) ◽  
pp. 15-24
Author(s):  
Shirin Ibrahim ◽  
◽  
MUHAMAD ANAS MARZUKE ◽  
ZUL HAZMI HUSSIN ◽  
NOR SHAHIDA KADER BASHAH ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Fibroblast Cell ◽  
Phosphate Powder ◽  
L929 Fibroblast ◽  
Synthetic Calcium Phosphate

Efficacy of Ozonation Treatments of Smoked Fish for Reducing Its Benzo[a]pyrene Concentration and Toxicity

2016 ◽  
Vol 79 (12) ◽  
pp. 2167-2173 ◽  
Author(s):  
IRINA ROZENTALE ◽  
JANIS ANCANS ◽  
ELENA BARTKIENE ◽  
ARTURS VIKSNA ◽  
VADIMS BARTKEVICS
Keyword(s):  
Oxidative Degradation ◽  
Fibroblast Cell ◽  
Mouse Embryonic Fibroblast ◽  
Prolonged Treatment ◽  
In Vitro Toxicity ◽  
Smoked Fish ◽  
Standard Solution ◽  
Pyrene Concentration ◽  
The Impact

ABSTRACT Ozone is widely used in food processing, for example, to decompose mycotoxins or pesticide residues, to extend the shelf life of products, and for sanitation. The objective of this study was to assess the possibility of expanding the application of ozone for oxidative degradation of polycyclic aromatic hydrocarbons (PAHs). The evaluation was conducted by ozonation of a benzo[a]pyrene (BaP) standard solution and smoked fish (sprats) contaminated with PAHs. The effect of ozonation was immediate in the BaP solution; 89% of this toxic compound was decomposed after only 1 min of treatment. However, the impact of ozonation on the smoked sprats was less pronounced, even after prolonged treatment. The final reduction in benzo[b]fluoranthene and BaP concentrations in smoked sprats contaminated with PAHs was 34 and 46%, respectively, after 60 min of ozonation, but no significant decrease of benzo[a]anthracene and chrysene concentrations was observed. To evaluate the safety of ozonation, the toxicity of the ozone-treated BaP standard solution was investigated. In vitro toxicity was evaluated using human hepatocellular carcinoma and mouse embryonic fibroblast cell lines as models. The cytotoxicity of the BaP standard solution significantly increased after ozonation, indicating a pronounced negative effect in terms of food safety.

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