GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE): IV. ELECTROPHORETIC BANDING PATTERNS OF OCTANOL DEHYDROGENASE AND ARGININE PHOSPHOKINASE

1979 ◽  
Vol 111 (11) ◽  
pp. 1307-1310 ◽  
Author(s):  
R.H. Gooding ◽  
B.M. Rolseth
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Aldehyde Oxidase ◽  
Glossina Morsitans Morsitans ◽  
Polyacrylamide Gel ◽  
Single Locus ◽  
Single Individual ◽  
Glossina Morsitans ◽  
Banding Patterns ◽  
Octanol Dehydrogenase

AbstractPolyacrylamide gel electrophoresis of the thoraces of adult Glossina morsitans morsitans Westwood revealed five octanol dehydrogenase (ODH, E.C. 1.1.1.73) phenotypes (and a sixth was predicted) in males and females, two arginine phosphokinase (APK, E.C. 2.7.3.3) phenotypes in males and three APK phenotypes in females. Each enzyme was postulated to be under the control of a single locus; Odh with three alleles on an autosome and Apk with two alleles on the X-chromosome. Allele frequencies were in Hardy-Weinberg equilibrium in our colony, and breeding experiments provided direct evidence for single locus control of each enzyme. The polyacrylamide gel electrophoresis technique described permits determination of banding patterns for xanthine oxidase, aldehyde oxidase, ODH, and APK from a single individual.

GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE): II. ELECTROPHORETIC BANDING PATTERNS OF MIDGUT ALKALINE PHOSPHATASE

1978 ◽  
Vol 110 (12) ◽  
pp. 1241-1246 ◽  
Author(s):  
R. H. Gooding ◽  
B. M. Rolseth
Keyword(s):  
Alkaline Phosphatase ◽  
Glossina Morsitans Morsitans ◽  
Single Locus ◽  
Glossina Morsitans ◽  
Gene Frequencies ◽  
Ph Optimum ◽  
Banding Patterns ◽  
Laboratory Populations ◽  
Hardy Weinberg Equilibrium ◽  
Alkaline Phophatase

AbstractThe digestive section of the midgut of Glossina morsitans morsitans Westwood contains a phosphatase with a pH optimum of approximately 9.2 and with low substrate specificity; the enzyme was classified as an alkaline phosphatase (E.C. 3.1.3.1).Polyacrylamide gel (6%) electrophoresis (at pH 8.9) of the digestive portion of the midguts of adult G. morsitans morsitans revealed three alkaline phophatase phenotypes. Midgut phosphatase was postulated to be under control of a single locus (designated alkph) with two alleles. Gene frequencies were in Hardy-Weinberg equilibrium in two laboratory populations while a third, highly inbred population had only one phenotype. Phenotype frequencies were not significantly different among females of various ages from the Edmonton colony. Breeding experiments provided direct evidence for single locus control of midgut phosphatase.

Electrophoretic evidence supporting self-incompatibility in Lotus corniculatus

1980 ◽  
Vol 58 (6) ◽  
pp. 712-716 ◽  
Author(s):  
Shirley Dobrofsky ◽  
W. F. Grant
Keyword(s):  
Gel Electrophoresis ◽  
Seed Set ◽  
Polyacrylamide Gel Electrophoresis ◽  
Lotus Corniculatus ◽  
Polyacrylamide Gel ◽  
Self Incompatibility ◽  
Banding Patterns ◽  
Protein Subunits ◽  
Self Pollination

Self-incompatibility, a prefertilization event, and self-sterility, a postfertilization event, have both been suggested as causes for differences in seed set between cross- and self-pollinated florets in Lotus corniculatus L. Ovary protein subunits of selfed, crossed, and unpollinated florets of L. corniculatus cv. Mirabel were studied using polyacrylamide gel electrophoresis. Banding patterns differed for all three conditions. Ovary protein differences were found prior to the time fertilization is known to occur, thereby providing evidence that self-incompatibility is at least partially responsible for the reduced seed set after self-pollination.

Isozymes as markers for identification of tissue culture and greenhouse-grown strawberry cultivars

1991 ◽  
Vol 71 (4) ◽  
pp. 1195-1201 ◽  
Author(s):  
N. S. Nehra ◽  
K. K. Kartha ◽  
C. Stushnoff
Keyword(s):  
Tissue Culture ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Meristem Culture ◽  
Banding Patterns ◽  
Extraction Buffer ◽  
Leaf Tissues ◽  
The Stability ◽  
Strawberry Cultivars

Polyacrylamide gel electrophoresis (PAGE) was used for analysis of isozyme banding patterns of leucine aminopeptidase (LAP), phosphoglucomutase (PGM), phosphoglucoisomerase (PGI), esterase (EST) and 6-phosphogluconate dehydrogenase (6-PGD) in strawberry leaves. The extracts prepared from young leaf tissues using polytron homogenization and an extraction buffer containing 15 mg ml−1 dithiothreitol (DTT) and 10% insoluble polyvinylpyrrolidone (PVP-6755) gave best resolution for these enzymes. The influence of plant age and various growing environments on the stability of isozyme phenotypes was examined. The isozyme banding patterns of 6-PGD were found to vary with the change in growing environment as well as age of the plants. EST produced different banding patterns in greenhouse and tissue culture leaves. However, the isozyme phenotypes of LAP, PGM and PGI remained stable under all the conditions tested. Using a combination of these three stable enzymes, it was possible to distinguish eight strawberry cultivars under both tissue culture and greenhouse conditions. Key words: Fragaria × ananassa Duch., meristem culture, polyacrylamide gel electrophoresis

GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE): I. ELECTROPHORETIC BANDING PATTERNS OF XANTHINE OXIDASE AND ALDEHYDE OXIDASE

1978 ◽  
Vol 110 (12) ◽  
pp. 1233-1239 ◽  
Author(s):  
B.M. Rolseth ◽  
R.H. Gooding
Keyword(s):  
Xanthine Oxidase ◽  
Aldehyde Oxidase ◽  
Glossina Morsitans Morsitans ◽  
Single Locus ◽  
Glossina Morsitans ◽  
Gene Frequencies ◽  
University Of Alberta ◽  
Laboratory Populations ◽  
University Of Bristol ◽  
The University

AbstractPolyacrylamide gel (6%) electrophoresis (at pH 8.2) of the thoraces of adult Glossina morsitans morsitans Westwood revealed three xanthine oxidase (XO) phenotypes and six aldehyde oxidase (AO) phenotypes. Each enzyme was postulated to be under the control of a single locus, XO with two alleles and AO with three alleles. Gene frequencies were in Hardy-Weinberg equilibrium for both loci in two laboratory populations. Breeding experiments provided direct evidence for single locus control of each enzyme. No significant differences in phenotype frequencies were observed between the colony maintained at the University of Alberta, Canada and the parent colony at the University of Bristol, England. A colony of highly inbred flies from the University of Bristol had only one phenotype of AO and of XO.

GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE). V. DEFINITION AND PRELIMINARY MAPPING OF LINKAGE GROUPS I AND II

1981 ◽  
Vol 23 (3) ◽  
pp. 399-403 ◽  
Author(s):  
R. H. Gooding
Keyword(s):  
Linkage Group ◽  
X Chromosome ◽  
Aldehyde Oxidase ◽  
Glossina Morsitans Morsitans ◽  
Linkage Groups ◽  
Glossina Morsitans ◽  
Eye Color ◽  
Group I ◽  
Octanol Dehydrogenase ◽  
Differential Part

Linkage group I is defined as the loci on the differential part of the X-chromosome of adult Glossina morsitans morsitans Westwood. Three loci are known and their order on the X-chromosome has been demonstrated as ocra (body color), salmon (eye color), and Apk (arginine phosphokinase, E.C. 2.7.3.3) with 38 map units separating the first two loci and 32 to 41 separating the second two. This region of the X-chromosome does not contain the chromosomal inversion known to occur in the Handeni line of G. m. morsitans. Linkage group II is defined as the autosome carrying the locus Xo (xanthine oxidase, E.C. 1.2.3.2), and it is demonstrated to carry also the loci Ao (aldehyde oxidase, E.C. 1.2.3.1) and Odh (octanol dehydrogenase, E.C. 1.1.1.73). Ao and Odh are within 0.36 map units of each other and have not been separated by recombination; this pair of loci occur about 48 map units from Xo. During mapping experiments, no evidence for genetical recombination was found in male G. m. morsitans.

GENETICS OF GLOSSINA MORSITANS MORSITANS (DIPTERA: GLOSSINIDAE). VI. MULTILOCUS COMPARISON OF THREE LABORATORY POPULATIONS

1982 ◽  
Vol 24 (1) ◽  
pp. 109-115 ◽  
Author(s):  
R. H. Gooding ◽  
B. M. Rolseth
Keyword(s):  
Malate Dehydrogenase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Aldehyde Oxidase ◽  
Glossina Morsitans Morsitans ◽  
Reproductive Capacity ◽  
Glossina Morsitans ◽  
Geographic Origins ◽  
Laboratory Populations ◽  
The Mean ◽  
Glucose 6 Phosphate Dehydrogenase

Three strains (Kariba, Handeni, and ocra) of Glossina morsitans morsitans Westwood were examined by polyacrylamide gel electrophoresis to determine the extent of genetic similarity among the strains. Males were examined for 11 thoracic enzymes, one testicular enzyme, and one midgut enzyme. Tetrazolium oxidase, manganese stimulated malate dehydrogenase, an alpha-glycerophosophate deyhdrogenase, and testicular esterase were monomorphic. Variation was found in xanthine oxidase, aldehyde oxidase, octanol dehydrogenase, an alpha-glycerophosophate dehydrogenase, malate dehydrogenase, midgut alkaline phosphatase, two esterases, arginine phosphokinase, and glucose 6-phosphate dehydrogenase. Loci for the latter two enzymes are on the X-chromosome; all others are on autosomes. Allele frequencies in the three strains indicated that the Kariba and ocra strains are more closely related to each other than either is to the Handeni strain. These genetic similarities are consistant with the geographic origins of the strains. The mean heterozygosity per locus was highest (16.7% and 16.0%) in the two strains (Kariba and ocra) which have the highest reproductive capacity under laboratory conditions, and lowest (7.3%) in the strain (Handeni) which has the lowest reproductive capacity.

scholarly journals THE USE OF SEED PROTEIN BANDING PATTERNS FOR IDENTIFICATION OF PASTURE CULTIVARS

1988 ◽  
pp. 87-91
Author(s):  
M.B. Forde ◽  
S.E. Gardiner
Keyword(s):  
Gel Electrophoresis ◽  
Seed Protein ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Subterranean Clover ◽  
Seed Storage Proteins ◽  
Polyacrylamide Gel ◽  
Banding Patterns ◽  
Seed Certification ◽  
Plant Variety

Because of the growing number of pasture cultivars used in NZ and the difficulty of reliably separating cultivars of the same species by morphological characters, seed protein banding patterns have become a useful supplementary means of cultivar identification for the purposes of seed certification and plant variety rights applications. Sodium dodecylsufphate polyacrylamide gel electrophoresis of proteins extracted from ground seed samples produces distinctive patterns of bands representing seed storage proteins of different molecular weights. The procedure can be carried out in two days using viable or dead seed, and the results are not affected by site and season of growth. Although individual seeds of outbreeding species such as perennial ryegrass and white clover produce different banding patterns, the combined population representing the cultivar remains constant unless there has been genetic shift during seed multiplication. Speckes for whrch this procedure is being successfully used include the ryegrasses and fescues, browntop, cocksfoot, bromes, red and white clovers, subterranean clover, serradella and lotus. Even cultrvars as closely related as Nui and Ellett ryegrasses and Huia and Pitau white clovers can be separated by careful work. Because of minor technical differences between runs, all cultivars to be compared must be run on the same gel. Keywords: Seed certification, Plant variety rights, sodium dodecylsulphate polyacrylamide gel electrophoresis.

scholarly journals Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis

2008 ◽  
Vol 35 (2) ◽  
pp. 123-129 ◽  
Author(s):  
S. M. Gomez ◽  
N. N. Denwar ◽  
T. Ramasubramanian ◽  
Charles E. Simpson ◽  
G. Burow ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Leaf Tissue ◽  
Polyacrylamide Gel ◽  
Marker Allele ◽  
Breeding Programs ◽  
Banding Patterns ◽  
Use Of Resources ◽  
Effective Use ◽  
Band Separation

Abstract In peanut hybridization, distinguishing inadvertent selfs from the true hybrids may be difficult. In this study, to differentiate between selfs and hybrids, DNA was extracted from leaf tissue of F1 or F2 plants, and SSR markers were amplified and bands separated by a novel submarine horizontal polyacrylamide gel electrophoresis (H-PAGE). By comparing the resulting banding patterns to those of the parents, 70% of the putative hybrids were shown to be true hybrids on the basis of possessing a marker allele from the male parent. The H-PAGE gels gave better band separation and differentiation of selfed progenies than agarose gels, and were compatible with the common horizontal agarose gel units. This method provides a quick assay to distinguish hybrids from inadvertent selfs, and should result in greater efficiency and more effective use of resources in peanut breeding programs.

scholarly journals Lens neutral endopeptidase occurs in other bovine and human tissues

1987 ◽  
Vol 248 (3) ◽  
pp. 643-648 ◽  
Author(s):  
K Ray ◽  
H Harris
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Structural Difference ◽  
Double Diffusion ◽  
Neutral Endopeptidase ◽  
Relative Activity ◽  
Polyacrylamide Gel ◽  
Human Tissues ◽  
Banding Patterns ◽  
Bovine Lens

Lens neutral endopeptidase (EC 3.4.24.5) was previously thought to be unique to the eye lens. We report here the finding of a neutral endopeptidase, in a variety of bovine and human tissues, which is very similar both biochemically and immunologically to the lens endopeptidase. SDS/polyacrylamide-gel electrophoresis of partially purified enzyme fractions from various bovine tissues shows the characteristic pattern of at least eight bands with Mr values ranging from 24,000 to 32,000 which was described for the bovine-lens neutral endopeptidase. The relative activity of the enzyme varies from tissue to tissue with lung having the highest activity. Partially purified enzyme fractions from these tissues cross-react with antiserum raised in rabbit against bovine lens endopeptidase showing apparent identity when examined side by side in Ouchterlony double-diffusion tests. The human enzyme also cross-reacts with the antiserum but when tested by double-diffusion against the bovine enzyme the precipitin lines show spurring at the joining edges indicating a structural difference between the human and the bovine enzymes. It was also found by Western blot experiments, after denaturing polyacrylamide-gel electrophoresis of the enzyme, that the polypeptide components of the human and bovine enzymes show somewhat different banding patterns.

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