scholarly journals Murine Red Blood Cells Lack Ligands for B Cell Siglecs, Allowing Strong Activation by Erythrocyte Surface Antigens

2017 ◽  
Vol 200 (3) ◽  
pp. 949-956 ◽  
Author(s):  
Fernando Spiller ◽  
Corwin M. Nycholat ◽  
Chika Kikuchi ◽  
James C. Paulson ◽  
Matthew S. Macauley
Keyword(s):  
Red Blood Cells ◽  
B Cell ◽  
Blood Cells ◽  
Surface Antigens ◽  
Erythrocyte Surface

scholarly journals A quantitative methodology to detect surface antigens on red blood cells using optical cell-detachment technique

2020 ◽  
Author(s):  
CHIH-LANG LIN ◽  
SHYANG-GUANG WANG ◽  
MENG-TSUNG TIEN ◽  
CHUNG-HAN CHIANG ◽  
YI-CHIEH LEE ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Antigen Expression ◽  
Surface Antigens ◽  
Cell Detachment ◽  
Quantitative Methodology ◽  
Optical Cell ◽  
Surface Antigen Expression ◽  
Alternative Methodology ◽  
Coated Surface

Abstract The quantitative analysis of surface antigens on cells, especially red blood cells (RBCs), has attracted increasing attention due to the recognition of antigenic changes that can facilitate early diagnoses. This paper presents an alternative methodology developed using the optical cell-detachment technique to evaluate antibody-antigen interactions and quantitatively analyze the RBC surface antigen expression. RBC subtyping was used to verify the proposed detection principle based on a comparison of the bonding strengths between individual RBCs and antibody coatings. The bonding strengths were measured with serial antibody dilutions with gradually decreasing laser powers, for which a single cell was optically detached from the corresponding antibody-coated surface. With the quantitatively analysis, the proposed alternative methodology was verified as a highly sensitive technique for detecting antigen expression on the RBC surface.

scholarly journals Effects of trypsin on aggregation, disaggregation and aggregate morphology of red blood cells in autologous plasma and serum

2021 ◽  
Author(s):  
Yury Aleksandrovich Sheremet'ev
Keyword(s):  
Red Blood Cells ◽  
Surface Charge ◽  
Blood Cells ◽  
Cell Aggregates ◽  
Trypsin Treatment ◽  
Autologous Plasma ◽  
Erythrocyte Surface ◽  
Rbc Aggregation ◽  
Aggregate Morphology ◽  
Serum Trypsin

We study the influence of trypsin on aggregation, disaggregation, and aggregate morphology of RBCs in autologous plasma and serum. The effect of trypsin on the surface charge of red blood cells and the aggregation of glutaraldehyde fixed cells after treatment with the enzyme was also studied. RBC aggregation was studied by means of an aggregometer and microscopic observations. The results obtained in this study indicate that trypsin treatment increases RBCs aggregation in autologous plasma and serum. The disaggregation of erythrocytes after trypsin treatment considerably decreased in autologous plasma and serum. Increase in the strength of red blood cell aggregates was observed in autologous plasma and serum. The microscopic images of RBCs aggregates indicate the formation of globular (pathologic) structures of aggregates in autologous plasma and serum. Trypsin decrease the surface charge of RBCs. In autologous plasma and serum, the cup shapes of RBCs appear. The control RBCs fixed with glutaraldehyde were not aggregated after their placement in autologous plasma. At the same time, red blood cells pretreated with trypsin and fixed with glutaraldehyde interact with each other in autologous plasma. The physiological significance of glycoproteins of erythrocyte surface for RBCs aggregation was discussed.

THE NUMBER OF RED BLOOD CELLS IN THE ISLETS OF LANGERHANS AS AN INDEX OF THE B CELL ACTIVITY

1960 ◽  
Vol XXXIV (IV) ◽  
pp. 611-618 ◽  
Author(s):  
Claes Hellerström ◽  
Sighild Westman ◽  
Ulla Zachrisson ◽  
Bo Hellman
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
B Cell ◽  
Islets Of Langerhans ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Cell Function ◽  
Cell Activity ◽  
Control Group ◽  
Cell Content

ABSTRACT The number of red blood cells in the islets of Langerhans has been considered as a useful index of the insulin synthesis, i. e. of the functional state of the B cells of the islets. It was found, however, that in starved rats which had only received water orally, the red blood cell content within the islets increased both absolutely as well as relatively to that in the exocrine parenchyma at the same time as the decrease in the B cell function as estimated by caryometry. There was a marked correlation, both in the control group and in the starved rats, between the red blood cell concentration in the exocrine and endocrine parts of the pancreas. The extent to which a rise in the functional activity of the A cells might have contributed to the increased content of red blood cells in the islets during starvation is discussed.

scholarly journals Cross-reactive lysis of trinitrophenyl (TNP)-derivatized H-2 incompatible target cells by cytolytic T lymphocytes generated against syngeneic TNP spleen cells.

1976 ◽  
Vol 144 (6) ◽  
pp. 1609-1620 ◽  
Author(s):  
S J Burakoff ◽  
R N Germain ◽  
B Benacerraf
Keyword(s):  
T Lymphocytes ◽  
Red Blood Cells ◽  
Target Cell ◽  
Blood Cells ◽  
Surface Antigens ◽  
Lymphoid Cells ◽  
Target Cells ◽  
Cytolytic T Lymphocytes ◽  
Spleen Cells ◽  
Normal Spleen

Normal spleen cells, when cultured with irradiated trinitrophenyl (TNP)-derivatized syngeneic spleen cells, develop cytotoxic effectors that lyse most effectiviely a TNP-derivatized target that is H-2 compatible with the effector. However, these effectors also lyse to a lesser extent TNP tumor and TNP spleen targets that are H-2 incompatible. This cross-reactive lysis correlates with the degree of cytolysis seen on the TNP-derivatized syngeneic target; it appears to be medicated by Thy 1.2-bearing cells and is inhibited by antisera to the K and/or D loci of the target cell and not by antisera to non-K or non-D surface antigens. Nonradiolabeled TNP-derivatized lymphoid cells syngeneic to either the stimulator or the target are able to competitively inhibit cross-reactive lysis, while TNP chicken red blood cells are unable to specifically inhibit lysis. These data on cross-reactive lysis of TNP-conjugated targets are most consistent with the altered-self hypothesis.

Induction of autoantibodies to red blood cells by polyclonal B-cell activators

Nature ◽  
1976 ◽  
Vol 263 (5572) ◽  
pp. 60-61 ◽  
Author(s):  
LENNART HAMMARSTRÖM ◽  
EDVARD SMITH ◽  
DANIELE PRIMI ◽  
GÖRAN MÖLLER
Keyword(s):  
Red Blood Cells ◽  
B Cell ◽  
Blood Cells

scholarly journals In Vivo Removal of Malaria Parasites From Red Blood Cells Without Their Destruction in Acute Falciparum Malaria

Blood ◽  
1997 ◽  
Vol 90 (5) ◽  
pp. 2037-2040 ◽  
Author(s):  
Brian J. Angus ◽  
Kesinee Chotivanich ◽  
Rachanee Udomsangpetch ◽  
Nicholas J. White
Keyword(s):  
Red Blood Cells ◽  
Falciparum Malaria ◽  
Surface Antigen ◽  
Blood Cells ◽  
Malaria Infection ◽  
Infected Erythrocyte ◽  
Intracellular Parasites ◽  
Erythrocyte Surface

Abstract During acute falciparum malaria infection, red blood cells (RBC) containing abundant ring-infected erythrocyte surface antigen (Pf 155 or RESA), but no intracellular parasites, are present in the circulation. These RESA-positive parasite negative RBC are not seen in parasite cultures in vitro. This indicates that in acute falciparum malaria there is active removal of intraerythrocytic parasites by a host mechanism in vivo (probably the spleen) without destruction of the parasitized RBC. This may explain the observed disparity between the drop in hematocrit and decrease in parasite count in some hyperparasitemic patients. The fate of these “once-parasitized” RBC in vivo is not known.

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