scholarly journals Effects of trypsin on aggregation, disaggregation and aggregate morphology of red blood cells in autologous plasma and serum

2021 ◽  
Author(s):  
Yury Aleksandrovich Sheremet'ev
Keyword(s):  
Red Blood Cells ◽  
Surface Charge ◽  
Blood Cells ◽  
Cell Aggregates ◽  
Trypsin Treatment ◽  
Autologous Plasma ◽  
Erythrocyte Surface ◽  
Rbc Aggregation ◽  
Aggregate Morphology ◽  
Serum Trypsin

We study the influence of trypsin on aggregation, disaggregation, and aggregate morphology of RBCs in autologous plasma and serum. The effect of trypsin on the surface charge of red blood cells and the aggregation of glutaraldehyde fixed cells after treatment with the enzyme was also studied. RBC aggregation was studied by means of an aggregometer and microscopic observations. The results obtained in this study indicate that trypsin treatment increases RBCs aggregation in autologous plasma and serum. The disaggregation of erythrocytes after trypsin treatment considerably decreased in autologous plasma and serum. Increase in the strength of red blood cell aggregates was observed in autologous plasma and serum. The microscopic images of RBCs aggregates indicate the formation of globular (pathologic) structures of aggregates in autologous plasma and serum. Trypsin decrease the surface charge of RBCs. In autologous plasma and serum, the cup shapes of RBCs appear. The control RBCs fixed with glutaraldehyde were not aggregated after their placement in autologous plasma. At the same time, red blood cells pretreated with trypsin and fixed with glutaraldehyde interact with each other in autologous plasma. The physiological significance of glycoproteins of erythrocyte surface for RBCs aggregation was discussed.

Effects of contrast media on erythrocyte aggregation during sedimentation

2003 ◽  
Vol 81 (4) ◽  
pp. 397-404 ◽  
Author(s):  
Xuequn Huang ◽  
Akio Yoshikoshi ◽  
Kunihiro Hirano ◽  
Akio Sakanishi
Keyword(s):  
Contrast Media ◽  
Stationary Phase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Erythrocyte Aggregation ◽  
Collision Rate ◽  
Autologous Plasma ◽  
Erythrocyte Sedimentation ◽  
Rbc Aggregation ◽  
In Cells

To evaluate the effects of contrast media (CMs) on erythrocyte aggregation, we measured the erythrocyte sedimentation with Westergren method at 25°C. CMs were diatrizoate (Urografin® 76%) for ionic CM and iopamidol (Iopamiron® 370) for nonionic CM. Swine red blood cells (RBCs) were suspended in autologous plasma containing diatrizoate (URO), iopamidol (IOP), and saline (SAL) at 6.7% w/w, as well as in plasma alone (PLA), at 40% of the hematocrit. Sigmoid sedimentation curves were fitted to the Puccini et al. (1977) equation, and the average number of RBCs per aggregate m was calculated by Stokes' law against the time t. According to the Murata–Secomb (1988) theory we estimated the collision rate K between two aggregates from dm/dt in the stationary phase during sedimentation. Corresponding to the maximal ESR, the dm/dt (in cells/s) was 0.52 in PLA, 0.09 in SAL, 0.06 in URO and 0.03 in IOP, so that K also decreased in proportion to dm/dt from 145 fL/s in PLA to 8 fL/s in IOP. Both the ionic and nonionic CMs tend to inhibit the RBC aggregation more than that in SAL; the latter iopamidol appears to be inhibitory more than the former diatrizoate in autologous plasma.Key words: erythrocyte sedimentation, RBC aggregation, contrast media, diatrizoate, iopamidol.

Surface charge topography in ATP-depleted red blood cells

1985 ◽  
Vol 17 (2) ◽  
pp. 164
Author(s):  
Y. Marikovsky ◽  
R.S. Weinstein ◽  
E. Skutelsky ◽  
D. Danon
Keyword(s):  
Red Blood Cells ◽  
Surface Charge ◽  
Blood Cells

scholarly journals Necrotic and apoptotic volume changes of red blood cells investigated by low-angle light scattering technique

2007 ◽  
Vol 21 (2) ◽  
pp. 105-120 ◽  
Author(s):  
Igor V. Mindukshev ◽  
Vladimir V. Krivoshlyk ◽  
Elena E. Ermolaeva ◽  
Irina A. Dobrylko ◽  
Evgeniy V. Senchenkov ◽  
...  
Keyword(s):  
Light Scattering ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Platelets ◽  
Calcium Ionophore ◽  
Scattering Technique ◽  
Rbc Aggregation ◽  
Ammonia Medium ◽  
Very High ◽  
Light Scattering Technique

A low-angle light scattering technique, which has been applied previously to studies of blood platelets and Ehrlich ascite tumor cells, revealed differences in the dynamics of necrotic and apoptotic red blood cell death. Under hypotonic loading or in ammonia medium, red blood cells (RBC) swelled to a critical size (diameter approximately 13μm) prior to hemolysis (necrosis). Under acidic loading, hemolysis occurred with less pronounced swelling of cells (diameter approximately 10μm). Apoptosis induced by a calcium ionophore resulted in initial formation of echinocytes, followed by development of rounded red blood cells with uneven membrane, capable of agglomeration. In such a way, RBC aggregation can precede the final stages of the RBC apoptosis when small cellular fragments are generated. On the basis of erythrograms of the cells hemolysing in ammonia medium, the echinocytic (preapoptotic) and stomatocytic (prenecrotic) RBC were discerned due to the very high resistance of apoptotic RBC to osmotic (ammonia) loading.

Relations between Surface Charge and in Vitro Lysis of Red Blood Cells in Paroxysmal Nocturnal Haemoglobinuria

2009 ◽  
Vol 20 (2) ◽  
pp. 141-146 ◽  
Author(s):  
Lawrence L. Hause ◽  
Susan M. Koethe ◽  
David J. Rothwell ◽  
Jon V. Straumfjord
Keyword(s):  
Red Blood Cells ◽  
Surface Charge ◽  
Blood Cells ◽  
Paroxysmal Nocturnal Haemoglobinuria

Surface Charge of Red Blood Cells in Insulin-dependent Diabetic Patients with Incipient and Overt Nephropathy

1987 ◽  
Vol 4 (5) ◽  
pp. 431-433
Author(s):  
E. R. Mathiesen ◽  
C. Smith ◽  
M. Lauritzen ◽  
E. Hommel ◽  
M. Levin ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Surface Charge ◽  
Blood Cells ◽  
Diabetic Patients ◽  
Overt Nephropathy ◽  
Insulin Dependent ◽  
Insulin Dependent Diabetic

scholarly journals A Flow Induced Autoimmune Response and Accelerated Senescence of Red Blood Cells in Cardiovascular Devices

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
James P. Buerck ◽  
Dustin K. Burke ◽  
David W. Schmidtke ◽  
Trevor A. Snyder ◽  
Dimitrios Papavassiliou ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Conformational Changes ◽  
Blood Cells ◽  
Heart Valves ◽  
Autoimmune Response ◽  
Mechanical Trauma ◽  
Prosthetic Heart Valves ◽  
Autologous Plasma ◽  
Cardiovascular Devices ◽  
Cardiovascular Patients

AbstractRed blood cells (RBCs) passing through heart pumps, prosthetic heart valves and other cardiovascular devices undergo early senescence attributed to non-physiologic forces. We hypothesized that mechanical trauma accelerates aging by deformation of membrane proteins to cause binding of naturally occurring IgG. RBCs isolated from blood of healthy volunteers were exposed to high shear stress in a viscometer or microfluidics channel to mimic mechanical trauma and then incubated with autologous plasma. Increased binding of IgG was observed indicating forces caused conformational changes in a membrane protein exposing an epitope(s), probably the senescent cell antigen of band 3. The binding of immunoglobulin suggests it plays a role in the premature sequestration and phagocytosis of RBCs in the spleen. Measurement of IgG holds promise as a marker foreshadowing complications in cardiovascular patients and as a means to improve the design of medical devices in which RBCs are susceptible to sublethal trauma.

scholarly journals Cholesterol transport between red blood cells and lipoproteins contributes to cholesterol metabolism in blood

2020 ◽  
Vol 61 (12) ◽  
pp. 1577-1588
Author(s):  
Ryunosuke Ohkawa ◽  
Hann Low ◽  
Nigora Mukhamedova ◽  
Ying Fu ◽  
Shao-Jui Lai ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Cholesterol Efflux ◽  
Cholesterol Metabolism ◽  
Plasma Cholesterol ◽  
Translocator Protein ◽  
Plasma Lipoproteins ◽  
Cholesterol Transport ◽  
Autologous Plasma

Lipoproteins play a key role in transport of cholesterol to and from tissues. Recent studies have also demonstrated that red blood cells (RBCs), which carry large quantities of free cholesterol in their membrane, play an important role in reverse cholesterol transport. However, the exact role of RBCs in systemic cholesterol metabolism is poorly understood. RBCs were incubated with autologous plasma or isolated lipoproteins resulting in a significant net amount of cholesterol moved from RBCs to HDL, while cholesterol from LDL moved in the opposite direction. Furthermore, the bi-directional cholesterol transport between RBCs and plasma lipoproteins was saturable and temperature-, energy-, and time-dependent, consistent with an active process. We did not find LDLR, ABCG1, or scavenger receptor class B type 1 in RBCs but found a substantial amount of ABCA1 mRNA and protein. However, specific cholesterol efflux from RBCs to isolated apoA-I was negligible, and ABCA1 silencing with siRNA or inhibition with vanadate and Probucol did not inhibit the efflux to apoA-I, HDL, or plasma. Cholesterol efflux from and cholesterol uptake by RBCs from Abca1+/+ and Abca1−/− mice were similar, arguing against the role of ABCA1 in cholesterol flux between RBCs and lipoproteins. Bioinformatics analysis identified ABCA7, ABCG5, lipoprotein lipase, and mitochondrial translocator protein as possible candidates that may mediate the cholesterol flux. Together, these results suggest that RBCs actively participate in cholesterol transport in the blood, but the role of cholesterol transporters in RBCs remains uncertain.

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