scholarly journals Trypanosoma cruzi Coexpressing Ornithine Decarboxylase and Green Fluorescence Proteins as a Tool to Study the Role of Polyamines in Chagas Disease Pathology

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Jeremías José Barclay ◽  
Luciano Gastón Morosi ◽  
María Cristina Vanrell ◽  
Edith Corina Trejo ◽  
Patricia Silvia Romano ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Trypanosoma Cruzi ◽  
Chagas Disease ◽  
Ornithine Decarboxylase ◽  
Fluorescent Protein ◽  
Selectable Marker ◽  
Green Fluorescence ◽  
Parasite Survival ◽  
Green Fluorescent

Polyamines are essential for Trypanosoma cruzi, the causative agent of Chagas disease. As T. cruzi behaves as a natural auxotrophic organism, it relies on host polyamines biosynthesis. In this paper we obtained a double-transfected T. cruzi parasite that expresses the green fluorescent protein (GFP) and a heterologous ornithine decarboxylase (ODC), used itself as a novel selectable marker. These autotrophic and fluorescent parasites were characterized; the ODC presented an apparent Km for ornithine of 0.51 ± 0.16 mM and an estimated Vmax value of 476.2 nmoles/h/mg of protein. These expressing ODC parasites showed higher metacyclogenesis capacity than the auxotrophic counterpart, supporting the idea that polyamines are engaged in this process. This double-transfected T. cruzi parasite results in a powerful tool—easy to follow by its fluorescence—to study the role of polyamines in Chagas disease pathology and in related processes such as parasite survival, invasion, proliferation, metacyclogenesis, and tissue spreading.

Defining the Role of Arginine 96 in Green Fluorescent Protein Fluorophore Biosynthesis†,‡

Biochemistry ◽  
2005 ◽  
Vol 44 (49) ◽  
pp. 16211-16220 ◽  
Author(s):  
Timothy I. Wood ◽  
David P. Barondeau ◽  
Chiharu Hitomi ◽  
Carey J. Kassmann ◽  
John A. Tainer ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

scholarly journals Identification of Recombinant Baculoviruses Using Green Fluorescent Protein as a Selectable Marker

BioTechniques ◽  
1997 ◽  
Vol 22 (4) ◽  
pp. 674-681 ◽  
Author(s):  
L.E. Wilson ◽  
N. Wilkinson ◽  
S.A. Marlow ◽  
R.D. Possee ◽  
L.A. King
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Selectable Marker ◽  
Recombinant Baculoviruses ◽  
Green Fluorescent

scholarly journals ROLE OF GREEN-FLUORESCENT-PROTEIN-EXPRESSING T CELLS IN EXPERIMENTAL AUTOIMMUNE MYOCARDITIS

2011 ◽  
Vol 57 (14) ◽  
pp. E228
Author(s):  
Tomoyoshi Yanagisawa ◽  
Takayuki Inomata ◽  
Ichiro Watanabe ◽  
Emi Maekawa ◽  
Tomohiro Mizutani ◽  
...  
Keyword(s):  
T Cells ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Autoimmune Myocarditis ◽  
Experimental Autoimmune Myocarditis ◽  
Green Fluorescent ◽  
Experimental Autoimmune

scholarly journals Experimental Model of Human Body Louse Infection Using Green Fluorescent Protein-Expressing Bartonella quintana

2001 ◽  
Vol 69 (3) ◽  
pp. 1876-1879 ◽  
Author(s):  
Pierre-Edouard Fournier ◽  
Michael F. Minnick ◽  
Hubert Lepidi ◽  
Eric Salvo ◽  
Didier Raoult
Keyword(s):  
Green Fluorescent Protein ◽  
Human Body ◽  
Fluorescent Protein ◽  
Rabbit Model ◽  
Bartonella Quintana ◽  
Laboratory Colony ◽  
Green Fluorescent ◽  
Human Body Louse ◽  
Body Lice

ABSTRACT A laboratory colony of human body lice was experimentally infected by feeding on rabbits made artificially bacteremic with a green fluorescent protein-expressing Bartonella quintana. B. quintana was detected in the gut and feces until death but not in the eggs. The life span of the lice was not modified. The rabbit model should provide valuable clues to the role of lice in the transmission of B. quintana.

scholarly journals A fine structure study of the anthocodium in Renilla mülleri. Evidence for the existence of a bioluminescent organelle, the luminelle.

1975 ◽  
Vol 64 (1) ◽  
pp. 15-28 ◽  
Author(s):  
B O Spurlock ◽  
M J Cormier
Keyword(s):  
Fine Structure ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Structure Study ◽  
Green Fluorescence ◽  
Subcellular Structure ◽  
Subcellular Organelle ◽  
Green Fluorescent ◽  
Structural Entity

A fine structure study of the anthocodium of the sea pansy, Renilla mülleri, was undertaken. The anthocodium, a known site of bioluminescence, was selected in order to determine whether a structural entity could be found which would satisfy the biochemical and physiological features associated with the known sites of bioluminescence in this animal. These sites, termed lumisomes, have previously been shown to be small (0.1-0.2 mum), membrane-enclosed vesicles which contain all the proteins necessary for bioluminescence and its immediate control. One of the lumisomal proteins is an intensely green fluorescent protein and has been used as a probe for the detection of the cellular sites of bioluminescence. This green fluorescence was associated only with gastrodermal cells. We report the identification of a unique morphological entity, restricted to the cells of the gastrodermis, which satisfies the biochemical and physiological requirements for bioluminescence in Renilla. It is a large (4-6 mum), membrane-bounded subcellular organelle comparable in size to a subcellular structure whose green fluorescence is typically associated with the in vivo bioluminescence. Furthermore, it is filled with smaller membrane-bounded vesicles which have the same size and shape as the lumisomes. We suggest that the organelle identified in this study be termed a luminelle.

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