scholarly journals Hydrogel-based platforms to mimic in vivo drug diffusion: A multicenter research

2020 ◽  
Author(s):  
Paola Petrini ◽  
Daniela Pacheco ◽  
Francesco Briatico Vangosa ◽  
Cosmin S. Butnarasu ◽  
Livia Visai ◽  
...  
Keyword(s):  
High Throughput ◽  
Screening Tool ◽  
In Vitro Screening ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Drug Diffusion ◽  
Preclinical Trials ◽  
State Of Art

An airway mucus model is proposed thus serving as an in vitro screening tool with the aim to reduce the number of noneffective drugs reaching the preclinical trials. The engineered mucus model is an easy-to-use and easy-to-produce tool that can be easily coupled to state-of-art diffusion models and it is compatible with high throughput analysis. This platform will serve as the basis to implement the complexity of the model in terms of components, also including the effect of bacteria

scholarly journals Formulation, High Throughput In Vitro Screening and In Vivo Functional Characterization of Nanoemulsion-Based Intranasal Vaccine Adjuvants

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0126120 ◽  
Author(s):  
Pamela T. Wong ◽  
Pascale R. Leroueil ◽  
Douglas M. Smith ◽  
Susan Ciotti ◽  
Anna U. Bielinska ◽  
...  
Keyword(s):  
High Throughput ◽  
Functional Characterization ◽  
In Vitro Screening ◽  
Vaccine Adjuvants ◽  
Intranasal Vaccine

scholarly journals High-Throughput Screening of Novel Peptide Inhibitors of an Integrin Receptor from the Hexapeptide Library by Using a Protein Microarray Chip

2004 ◽  
Vol 9 (8) ◽  
pp. 687-694 ◽  
Author(s):  
Yoonsuk Lee ◽  
Dong-Ku Kang ◽  
Soo-Ik Chang ◽  
Moon Hi Han ◽  
In-Cheol Kang
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Competitive Inhibition ◽  
Protein Microarray ◽  
Dependent Manner ◽  
High Throughput Analysis ◽  
Microarray Chip ◽  
New Drug

Protein microarray is an emerging technology that makes high-throughput analysis possible for protein-protein interactions and analysis of proteome and biomarkers in parallel. The authors investigated the application of a novel protein microarray chip, Proteo Chip, in new drug discovery. Integrin αvβ3 microarray immobilized on the Proteo Chip was employed to screen new active peptides against the integrin from multiple hexapeptide sublibraries of a positional scanning synthetic peptide combinatorial library (PS-SPCL). The integrin αvβ3-vitronectin interaction was successfully demonstrated on the integrin microarray in a dose-dependent manner andwas inhibited not only by the syntheticRGDpeptide but also by various integrin antagonists on the integrin microarray chip. Novel peptide ligands with high affinity to the integrin were also identified from the peptide libraries with this chip-based screening system by a competitive inhibition assay in a simultaneous and highthroughput fashion. The authors have confirmed antiangiogenic functions of the novel peptides thus screened through an in vitro and in vivo angiogenesis assay. These results provide evidence that the Proteo Chip is a promising tool for highthroughput screening of lead molecules in new drug development.

scholarly journals An in vitro assay for clonogenic, high‐throughput analysis of intestinal stem cells

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Adam David Gracz ◽  
Michael J Johnston ◽  
Fengchao Wang ◽  
Ian A Williamson ◽  
Yuli Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
High Throughput ◽  
In Vitro Assay ◽  
Intestinal Stem Cells ◽  
High Throughput Analysis ◽  
Vitro Assay ◽  
Throughput Analysis

Quantitative PCR-based approach for rapid phage display analysis: a foundation for high throughput vascular proteomic profiling

2006 ◽  
Vol 26 (3) ◽  
pp. 202-208 ◽  
Author(s):  
Victoria L. T. Ballard ◽  
Jacquelyne M. Holm ◽  
Jay M. Edelberg
Keyword(s):  
Phage Display ◽  
Quantitative Pcr ◽  
High Throughput ◽  
Close Correlation ◽  
Proteomic Profiling ◽  
High Throughput Analysis ◽  
Vascular Beds ◽  
Primer Sets

Functional proteomic strategies offer unique advantages over current molecular array approaches, as the epitopes identified can directly provide bioactive peptides for investigational and/or translational applications. The vascular endothelium is well suited to proteomic assessment by in vivo phage display, but extensive enrichment and sequencing steps limit its application for high throughput molecular profiling. To overcome these limitations we developed a quantitative PCR (Q-PCR) strategy to allow the rapid quantification of in vivo phage binding. Primers were designed for distinct clones selected from a defined phage pool to probe for age-associated changes in cardiac vascular epitopes. Sensitivity and specificity of the primer sets were tested and confirmed in vitro. Q-PCR quantification of phage in vivo confirmed the preferential homing of all phage clones to the young rather than old cardiac vasculature and demonstrated a close correlation with phage measurements previously determined using traditional bacterial-based titration methods. This Q-PCR approach provides quantification of phage within hours of phage injection and may therefore be used for rapid, high throughput analysis of binding of defined phage sequences both in vivo and in vitro, complementing nonbiased phage approaches for the proteomic mapping of vascular beds and other tissues.

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