scholarly journals IVTech: The next generation of in vitro models

2021 ◽  
Vol 4 (s1) ◽  
Author(s):  
Tommaso Sbrana
Keyword(s):  
Cell Culture ◽  
In Vitro Model ◽  
Standard Equipment ◽  
The Poor ◽  
Human Reality ◽  
Vitro Model ◽  
In Vitro Cell Cultures ◽  
Technological Limitations ◽  
Different Tissues

In vitro cell cultures are often proposed as “Alternatives” to animal models, but they are still inadequate to reproduce the human pathophysiology. This is mainly due to the technological limitations of the standard equipment used in cell culture laboratories, such as the lack of a 3D micro-architecture, the static environment and the absence of cross talk between different tissues. These limitations cause the poor predictivity of a standard in-vitro model, if compared with the human reality.

Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)

2016 ◽  
Vol 133 ◽  
pp. 100-112 ◽  
Author(s):  
Victor Llombart ◽  
Teresa García-Berrocoso ◽  
Joan Josep Bech-Serra ◽  
Alba Simats ◽  
Alejandro Bustamante ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Stable Isotope ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Isotope Labeling ◽  
Vitro Model

SU-FF-J-104: In Vitro Model to Study the Biological Effect of Dose Gradients On a Cell Culture System

2005 ◽  
Vol 32 (6Part6) ◽  
pp. 1944-1944 ◽  
Author(s):  
R Bromley ◽  
L Oliver ◽  
R Harvie ◽  
R Davey
Keyword(s):  
Cell Culture ◽  
Biological Effect ◽  
Culture System ◽  
In Vitro Model ◽  
Cell Culture System ◽  
A Cell ◽  
Vitro Model

scholarly journals Isospora suis in an Epithelial Cell Culture System – An In Vitro Model for Sexual Development in Coccidia

PLoS ONE ◽  
2013 ◽  
Vol 8 (7) ◽  
pp. e69797 ◽  
Author(s):  
Hanna Lucia Worliczek ◽  
Bärbel Ruttkowski ◽  
Lukas Schwarz ◽  
Kirsti Witter ◽  
Waltraud Tschulenk ◽  
...  
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Sexual Development ◽  
Culture System ◽  
In Vitro Model ◽  
Cell Culture System ◽  
Epithelial Cell Culture ◽  
Isospora Suis ◽  
Vitro Model

scholarly journals A Pilot Study To Establish an In Vitro Model To Study Premature Intestinal Epithelium and Gut Microbiota Interactions

mSphere ◽  
2021 ◽  
Author(s):  
Justin Gibbons ◽  
Ji Youn Yoo ◽  
Tina Mutka ◽  
Maureen Groer ◽  
Thao T. B. Ho
Keyword(s):  
Cell Culture ◽  
In Vitro Model ◽  
Bacterial Flora ◽  
Mucosal Barrier ◽  
Clinical Observations ◽  
Culture Models ◽  
Vitro Model ◽  
Cell Culture Models

The gut bacterial flora influences the development of the immune system and long-term health outcomes in preterm infants. Studies of the mechanistic interactions between the gut bacteria and mucosal barrier are limited to clinical observations, animal models, and in vitro cell culture models for this vulnerable population.

Three-dimensional microtissues as an in vitro model for personalized radiation therapy

The Analyst ◽  
2017 ◽  
Vol 142 (19) ◽  
pp. 3605-3612 ◽  
Author(s):  
Yuting Qiu ◽  
Dandan Ning ◽  
Peipei Zhang ◽  
Stephanie Curly ◽  
Yong Qiao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Dna Damage ◽  
Animal Model ◽  
Radiation Therapy ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Intermediate Model ◽  
Radiation Induced ◽  
Vitro Model

This paper describes the use of 3D microtissues as an intermediate model between the 2D cell culture and the animal model to assess radiation-induced cellular and DNA damage in the context of personalized radiation therapy.

scholarly journals Considerations for a Reliable In Vitro Model of Chemotherapy-Induced Peripheral Neuropathy

Toxics ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 300
Author(s):  
Sandy Eldridge ◽  
Arianna Scuteri ◽  
Eugenia M. C. Jones ◽  
Guido Cavaletti ◽  
Liang Guo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Peripheral Neuropathy ◽  
Human Cell ◽  
Anticancer Agents ◽  
In Vitro Model ◽  
Neuronal Cell ◽  
Severe Toxicity ◽  
Human Cell Culture ◽  
Vitro Model

Chemotherapy-induced peripheral neuropathy (CIPN) is widely recognized as a potentially severe toxicity that often leads to dose reduction or discontinuation of cancer treatment. Symptoms may persist despite discontinuation of chemotherapy and quality of life can be severely compromised. The clinical symptoms of CIPN, and the cellular and molecular targets involved in CIPN, are just as diverse as the wide variety of anticancer agents that cause peripheral neurotoxicity. There is an urgent need for extensive molecular and functional investigations aimed at understanding the mechanisms of CIPN. Furthermore, a reliable human cell culture system that recapitulates the diversity of neuronal modalities found in vivo and the pathophysiological changes that underlie CIPN would serve to advance the understanding of the pathogenesis of CIPN. The demonstration of experimental reproducibility in a human peripheral neuronal cell system will increase confidence that such an in vitro model is clinically useful, ultimately resulting in deeper exploration for the prevention and treatment of CIPN. Herein, we review current in vitro models with a focus on key characteristics and attributes desirable for an ideal human cell culture model relevant for CIPN investigations.

Sign in / Sign up

Export Citation Format

Share Document