scholarly journals Alterations in chromosomal synapses and DNA repair in apoptotic spermatocytes of Mus m. domesticus

2016 ◽  
Vol 60 (2) ◽  
Author(s):  
E. Ayarza ◽  
M. González ◽  
F. López ◽  
R. Fernández-Donoso ◽  
J. Page ◽  
...  
Keyword(s):  
Dna Repair ◽  
Chromatin Remodeling ◽  
High Frequency ◽  
Caspase 9 ◽  
Autosomal Bivalents ◽  
Single Chromosome ◽  
Homologous Chromosomes ◽  
Synaptonemal Complexes ◽  
Dna Repair Proteins

<p>We investigated whether apoptotic spermatocytes from the mouse <em>Mus m. domesticus</em> presented alterations in chromosomal synapses and DNA repair. To enrich for apoptotic spermatocytes, the scrotum’s temperature was raised by partially exposing animals for 15 min to a 42ºC water bath. Spermatocytes in initial apoptosis were identified <em>in situ</em> by detecting activated Caspase-9.  SYCP1 and SYCP3 were markers for evaluating synapses or the structure of synaptonemal complexes and Rad51 and γH2AX for detecting DNA repair and chromatin remodeling. Apoptotic spermatocytes were concentrated in spermatogenic cycle stages III-IV (50.3%), XI-XII (44.1%) and IX-X (4.2%). Among apoptotic spermatocytes, 48% were in middle pachytene, 44% in metaphase and 6% in diplotene. Moreover, apoptotic spermatocytes showed several structural anomalies in autosomal bivalents, including splitting of chromosomal axes and partial asynapses between homologous chromosomes. gH2AX and Rad51 were atypically distributed during pachytene and as late as diplotene and associated with asynaptic chromatin, single chromosome axes or discontinuous chromosome axes. Among apoptotic spermatocytes at pachytene, 70% showed changes in the structure of synapses, 67% showed changes in gH2AX and Rad51 distribution and 50% shared alterations in both synapses and DNA repair. Our results showed that apoptotic spermatocytes from <em>Mus m. domesticus</em> contain a high frequency of alterations in chromosomal synapses and in the recruitment and distribution of DNA repair proteins. Together, these observations suggest that these alterations may have been detected by meiotic checkpoints triggering apoptosis.</p>

scholarly journals Cell Metabolism and DNA Repair Pathways: Implications for Cancer Therapy

2021 ◽  
Vol 9 ◽  
Author(s):  
Thais Sobanski ◽  
Maddison Rose ◽  
Amila Suraweera ◽  
Kenneth O’Byrne ◽  
Derek J. Richard ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Clinical Practice ◽  
Chromatin Remodeling ◽  
Metabolic Pathways ◽  
Cell Metabolism ◽  
Therapeutic Interventions ◽  
Dna Repair Proteins ◽  
Normal Human ◽  
Repair Pathways

DNA repair and metabolic pathways are vital to maintain cellular homeostasis in normal human cells. Both of these pathways, however, undergo extensive changes during tumorigenesis, including modifications that promote rapid growth, genetic heterogeneity, and survival. While these two areas of research have remained relatively distinct, there is growing evidence that the pathways are interdependent and intrinsically linked. Therapeutic interventions that target metabolism or DNA repair systems have entered clinical practice in recent years, highlighting the potential of targeting these pathways in cancer. Further exploration of the links between metabolic and DNA repair pathways may open new therapeutic avenues in the future. Here, we discuss the dependence of DNA repair processes upon cellular metabolism; including the production of nucleotides required for repair, the necessity of metabolic pathways for the chromatin remodeling required for DNA repair, and the ways in which metabolism itself can induce and prevent DNA damage. We will also discuss the roles of metabolic proteins in DNA repair and, conversely, how DNA repair proteins can impact upon cell metabolism. Finally, we will discuss how further research may open therapeutic avenues in the treatment of cancer.

scholarly journals DNA Environment of Centromeres and Non-Homologous Chromosomes Interactions in Mouse

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3375
Author(s):  
Victor Spangenberg ◽  
Mikhail Losev ◽  
Ilya Volkhin ◽  
Svetlana Smirnova ◽  
Pavel Nikitin ◽  
...  
Keyword(s):  
Y Chromosome ◽  
X Chromosome ◽  
Satellite Dna ◽  
Centromeric Region ◽  
Autosomal Bivalents ◽  
Homologous Chromosomes ◽  
Interdisciplinary Knowledge ◽  
Eukaryotic Genomes

Although the pericentromeric regions of chromosomes that are enriched in tandemly repeated satellite DNA represent a significant part of eukaryotic genomes, they remain understudied, which is mainly due to interdisciplinary knowledge gaps. Recent studies suggest their important role in genome regulation, karyotype stability, and evolution. Thus, the idea of satellite DNA as a junk part of the genome has been refuted. The integration of data regarding molecular composition, chromosome behaviour, and the details of the in situ organization of pericentromeric regions is of great interest. The objective of this work was a cytogenetic analysis of the interactions between pericentromeric regions from non-homologous chromosomes in mouse spermatocytes using immuno-FISH. We analysed two events: the associations between centromeric regions of the X chromosome and autosomes and the associations between the centromeric regions of the autosomal bivalents that form chromocenters. We concluded that the X chromosome forms temporary synaptic associations with different autosomes in early meiotic prophase I, which can normally be found until the pachytene–diplotene, without signs of pachytene arrest. These associations are formed between the satellite-DNA-rich centromeric regions of the X chromosome and different autosomes but do not involve the satellite-DNA-poor centromeric region of the Y chromosome. We suggest the hypothetical model of X chromosome competitive replacement from such associations during synaptic correction. We showed that the centromeric region of the X chromosome in association remains free of γH2Ax-dependent chromatin inactivation, while the Y chromosome is completely inactivated. This finding highlights the predominant role of associations between satellite DNA-rich regions of different chromosomes, including the X chromosome. We suppose that X-autosomal transient associations are a manifestation of an additional synaptic disorder checkpoint. These associations are normally corrected before the late diplotene stage. We revealed that the intense spreading conditions that were applied to the spermatocyte I nuclei did not lead to the destruction of stretched chromatin fibers of elongated chromocenters enriched in satellite DNA. The tight associations that we revealed between the pericentromeric regions of different autosomal bivalents and the X chromosome may represent the basis for a mechanism for maintaining the repeats stability in the autosomes and in the X chromosome. The consequences of our findings are discussed.

In-situ compaction and sintering of Al2O3 – GNP nanoparticles using a high-frequency induction system

2018 ◽  
Vol 60 (7-8) ◽  
pp. 727-732
Author(s):  
Uğur Çavdar ◽  
İ. Murat Kusoglu ◽  
Ayberk Altintas
Keyword(s):  
High Frequency ◽  
Induction System ◽  
High Frequency Induction

scholarly journals Location of agate geodes in Permian deposits of Simota gully using the GPR

2021 ◽  
Author(s):  
Michał Mierczak ◽  
Jerzy Karczewski
Keyword(s):  
Signal Processing ◽  
High Frequency ◽  
Electromagnetic Energy ◽  
Reflection Coefficients ◽  
Electric Permittivity ◽  
Pyroclastic Deposits ◽  
High Attenuation ◽  
Subsurface Geology ◽  
Study Test

AbstractThe article describes the establishment of the location of agate geodes using the GPR method in the area of the Simota gully (Lesser Poland Voivodeship). Agates (a multicolored variety of gemstone of chalcedony group) have multifaceted values that informed their study. Traditional methods of geode location are less reliable, hence the attempt to use the GPR method. Measurements were taken at two study test sites with subsurface geology of weathered melaphyre and pyroclastic deposits using a GPR system (ProEx). A high-frequency antenna (1.6 GHz) was used along with the pre-established profiles of lengths of 6-m and 10-cm intervals. Furthermore, simple soil tests using the soil sampler tool were made prior to the GPR measurement. The GPR results show significant high attenuation of the electromagnetic energy interpreted to be due to clay components of the regolith. Advanced signal processing procedures (such as the attribute of the signal) were used on the data for better enhancement that aided interpretation. Other anomalies depicted on the radargrams were thought to be the presence of roots, pieces of melaphyres-targeted agates. Furtherance to ascertain the reflection coefficients as recorded on the GPR data, in situ samples (root pieces, melaphyres, agates) taken were tested in the laboratory for electric permittivity property. Based on the interpretation results, several agate geodes were dug out from the ground.

A Demonstration of a 1:1 Correspondence Between Chiasma Frequency and Recombination Using a Lolium perenne/Festuca pratensis Substitution

Genetics ◽  
2002 ◽  
Vol 161 (1) ◽  
pp. 307-314 ◽  
Author(s):  
J King ◽  
L A Roberts ◽  
M J Kearsey ◽  
H M Thomas ◽  
R N Jones ◽  
...  
Keyword(s):  
Lolium Perenne ◽  
Chiasma Frequency ◽  
Chiasma Formation ◽  
Grass Species ◽  
Festuca Pratensis ◽  
Single Chromosome ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Frequency Counts

Abstract A single chromosome of the grass species Festuca pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line (2n = 2x = 14). The chromatin of F. pratensis and L. perenne can be distinguished by genomic in situ hybridization (GISH), and it is therefore possible to visualize the substituted F. pratensis chromosome in the L. perenne background and to study chiasma formation in a single marked bivalent. Recombination occurs freely in the F. pratensis/L. perenne bivalent, and chiasma frequency counts give a predicted map length for this bivalent of 76 cM. The substituted F. pratensis chromosome was also mapped with 104 EcoRI/Tru91 and HindIII/Tru91 amplified fragment length polymorphisms (AFLPs), generating a marker map of 81 cM. This map length is almost identical to the map length of 76 cM predicted from the chiasma frequency data. The work demonstrates a 1:1 correspondence between chiasma frequency and recombination and, in addition, the absence of chromatid interference across the Festuca and Lolium centromeres.

scholarly journals Lateral Elements Inside Synaptonemal Complex-Like Polycomplexes in ndt80 Mutants of Yeast Bind DNA

Genetics ◽  
2003 ◽  
Vol 163 (2) ◽  
pp. 539-544 ◽  
Author(s):  
Hasanuzzaman Bhuiyan ◽  
Gunilla Dahlfors ◽  
Karin Schmekel
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscope ◽  
Synaptonemal Complex ◽  
Immunoelectron Microscopy ◽  
Meiotic Prophase ◽  
Lateral Element ◽  
Homologous Chromosomes ◽  
Meiotic Prophase I ◽  
Dna Antibodies

Abstract The synaptonemal complex (SC) keeps the synapsed homologous chromosomes together during pachytene in meiotic prophase I. Structures that resemble stacks of SCs, polycomplexes, are sometimes found before or after pachytene. We have investigated ndt80 mutants of yeast, which arrest in pachytene. SCs appear normal in spread chromosome preparations, but are only occasionally found in intact nuclei examined in the electron microscope. Instead, large polycomplexes occur in almost every ndt80 mutant nucleus. Immunoelectron microscopy using DNA antibodies show strong preferential labeling to the lateral element parts of the polycomplexes. In situ hybridization using chromosome-specific probes confirms that the chromosomes in ndt80 mutants are paired and attached to the SCs. Our results suggest that polycomplexes can be involved in binding of chromosomes and possibly also in synapsis.

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