scholarly journals Histochemistry for nanomedicine: Novelty in tradition

2021 ◽  
Vol 65 (4) ◽  
Author(s):  
Manuela Malatesta
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Life Sciences ◽  
The Novel ◽  
Transmission Electron ◽  
Unequivocal Identification ◽  
Cell Components ◽  
Staining Methods ◽  
Great Development

During the last two centuries, histochemistry has provided significant advancements in many fields of life sciences. After a period of neglect due to the great development of biomolecular techniques, the histochemical approach has been reappraised and is now widely applied in the field of nanomedicine. In fact, the novel nanoconstructs intended for biomedical purposes must be visualized to test their interaction with tissue and cell components. To this aim, several long-established staining methods have been re-discovered and re-interpreted in an unconventional way for unequivocal identification of nanoparticulates at both light and transmission electron microscopy.

Investigation of the Morphology of Recombinant Von Willebrand Factor by Two High-Resolution Microscopic Techniques

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 1409-1409
Author(s):  
Hanspeter Rottensteiner ◽  
Birgit K Seyfried ◽  
Gernot Friedbacher ◽  
Guenter Allmaier ◽  
Nicola Ilk ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Life Sciences ◽  
Force Microscopy ◽  
Atomic Force ◽  
University Of Technology ◽  
Transmission Electron ◽  
Von Willebrand ◽  
Willebrand Factor ◽  
Mode Atomic Force Microscopy

Abstract Abstract 1409 Von Willebrand factor (VWF) is the largest known glycoprotein circulating in human blood plasma and is composed of a series of multimers with molecular weights ranging from 600 to 20,000 kDa or even more. In this study, we investigated the morphology of recombinant VWF (rVWF) and compared it to that of plasma-derived VWF (pdVWF) by tapping mode atomic force microscopy and transmission electron microscopy. Tapping mode atomic force microscopy showed that both rVWF and pdVWF contain globular and stretched domains. Mean chain lengths of the filaments and diameters of the core globular domains were determined and analyzed on a statistical basis. About 70% of the rVWF and pdVWF molecules were 100–300 nm long. The portion of very long molecules (>300 nm) was only slightly greater in rVWF than in pdVWF (20% versus 18%). The diameters of the globular core structures were in the range of 12 to 30 nm for both types of VWF. Inspection of a purified rVWF dimer revealed a similar range for the globular domain (14-32 nm). Upon exposure to high shear stress, a dramatic conformational change was observed for rVWF, just as has been reported for pdVWF. Investigation of negatively stained preparations of rVWF by transmission electron microscopy showed a highly organized structure. The heterogeneity in size observed was as expected for a polymeric VWF that consists of a mixture of multimers of varying sizes. The micrographs also revealed the presence of coiled and elongated structures. Analysis of pdVWF led to similar results, which were also in agreement with data from the literature. The results confirmed that the morphology of rVWF is similar to that observed for VWF purified from human normal plasma. Disclosures: Rottensteiner: Baxter Innovations GmbH: Employment. Seyfried:Baxter Innovations GmbH: Employment. Friedbacher:Vienna University of Technology: Consultancy. Allmaier:Vienna University of Technology: Consultancy. Ilk:University of Natural Resources and Applied Life Sciences: Consultancy. Sleytr:University of Natural Resources and Applied Life Sciences: Consultancy. Ehrlich:Baxter Innovations GmbH: Employment. Turecek:Baxter BioScience: Employment.

Fabrication of boron carbonitride (BCN) nanotubes and giant fullerene cages

2010 ◽  
Vol 88 (12) ◽  
pp. 1256-1261 ◽  
Author(s):  
Guifang Sun ◽  
Faming Gao ◽  
Li Hou
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
The Novel ◽  
X Ray Diffraction ◽  
Boron Carbonitride ◽  
X Ray ◽  
Transmission Electron ◽  
New Form ◽  
First Time ◽  
Electron Energy Loss

Boron carbonitride (BCN) nanotubes have been successfully prepared using NH4Cl, KBH4, and ZnBr2 as the reactants at 480 °C for 12 h by a new benzene-thermal approach in a N2 atmosphere. As its by-product, a new form of carbon regular hexagonal nanocages are observed. The samples are characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), transmission electron diffraction (TED), electron energy loss spectroscopy (EELS), and high-resolution transmission electron microscopy (HRTEM). The prepared nanotubes have uniform outer diameters in the range of 150 to 500 nm and a length of up to several micrometerss. The novel carbon hexagonal nanocages have a typical size ranging from 100 nm to 1.5 µm, which could be the giant fullerene cages of [Formula: see text] (N = 17∼148). So, high fullerenes are observed for the first time. The influences of reaction temperature and ZnBr2 on products and the formation mechanism of BCN nanotubes are discussed.

Visualization of Dendrimer Molecules by Transmission Electron Microscopy (TEM):  Staining Methods and Cryo-TEM of Vitrified Solutions

1998 ◽  
Vol 31 (18) ◽  
pp. 6259-6265 ◽  
Author(s):  
Catheryn L. Jackson ◽  
Henri D. Chanzy ◽  
Frank P. Booy ◽  
Bartholomew J. Drake ◽  
Donald A. Tomalia ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Transmission Electron ◽  
Staining Methods

Crystallography of the Mg2Y precipitates in a damping Mg–Cu–Mn–Zn–Y alloy

2016 ◽  
Vol 49 (6) ◽  
pp. 2031-2035 ◽  
Author(s):  
Xuefei Huang ◽  
Weigang Huang
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Orientation Relationship ◽  
Lattice Model ◽  
Coincidence Site Lattice ◽  
Reciprocal Space ◽  
The Novel ◽  
Site Lattice ◽  
Lattice Matching ◽  
Transmission Electron

The crystallography of the Mg2Y precipitates in an Mg–Cu–Mn–Zn–Y damping alloy has been characterized by transmission electron microscopy. The novel orientation relationship between Mg2Y and the α-Mg matrix was determined as [0001]p//[0001]α, 〈10{\overline 1}0〉p//〈2{\overline 1}{\overline 1}0〉αand 〈01{\overline 1}0〉p//〈11{\overline 2}0〉α. The precipitate exhibits a polygonal morphology with four pairs of facets, each of which is normal to a Δgvector in reciprocal space. The secondary constrained coincidence site lattice model was employed to examine the preference of each facet. The results show that a much better degree of lattice matching is realized across each facet than their vicinal orientations, indicating the energetically favoured feature of the interface. The configurations of the misfit-compensating dislocations across each interface were also calculated using the O-lattice model.

scholarly journals Unique Minilens Cooling System Results in a Compact TEM

2008 ◽  
Vol 16 (2) ◽  
pp. 10-13
Author(s):  
Dmitry Lysenkov ◽  
William C. Monigle
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Product Design ◽  
Cooling System ◽  
Semiconductor Industry ◽  
Life Sciences ◽  
Recent Advances ◽  
Transmission Electron ◽  
Small Room

Transmission electron microscopy (TEM) has been proven to be one of the most convenient and straight forward methods for resolving sub-nanometer structures. The use of these instruments in life sciences, materials sciences and in the semiconductor industry has contributed greatly to many significant advances. However, typical TEMs are very large, expensive and relatively complicated to operate. Fortunately, recent advances in TEM technology and product design have created a new TEM instrument that is very well suited to everyday applications in hospitals, universities and laboratories of all types. Able to fit in a small room, this new TEM does not require the expensive, extensive building modifications usually associated with a TEM installation.

Growth behavior and structures of carbon nanotubes

1993 ◽  
Vol 51 ◽  
pp. 752-753
Author(s):  
Mingqi Liu ◽  
John M. Cowley
Keyword(s):  
Electron Microscopy ◽  
Carbon Nanotubes ◽  
Transmission Electron Microscopy ◽  
Scanning Transmission Electron Microscopy ◽  
Arc Discharge ◽  
The Novel ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Potential Applications ◽  
Discharge Method

Recent discovery of the novel hollow graphitic tubules of nanometer dimensions, so called carbon nanotubes, has greatly stimulated the studies in the field of carbon fiber growth. Their potential applications in electronic and materials industries have been suggested. In the present study, carbon nanotubes were fabricated by an arc-discharge method. The microstructures of nanotubes thus obtained were examined with a JEM-2000FX high-resolution transmission electron microscopy (HRTEM) and with an electron nanodiffraction technique in a VG HB-5 scanning transmission electron microscopy (STEM).HRTEM images show that nanotubes, consisting of 3 to 30 carbon sheets, have a length up to 1 μm and a diameter of 6 to 26 nm. Some nanotubes have a symmetric uniform sheet spacing of 0.34 nm. Others show non-symmetric uneven fringes (Fig. 1), indicating that they may have a polyhedral cross section. Most nanotubes are closed by cone-like or polyhedral shaped caps (Figs. 2 and 3). The closure angle varies from 17 to 40° and the internal sheets have a tendency to be closed in pairs. Fig. 4 shows the image of a growing tubes.

scholarly journals Visualization of Meiotic Chromosomes by Negative-Stain Transmission Electron Microscopy: Preparation and Staining Methods Using Common Staining Reagents

2006 ◽  
Vol 12 (S02) ◽  
pp. 284-285
Author(s):  
MJ de la Cruz ◽  
S Lockett ◽  
K Nagashima
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Meiotic Chromosomes ◽  
Negative Stain ◽  
Transmission Electron ◽  
Staining Methods

Extended abstract of a paper presented at Microscopy and Microanalysis 2006 in Chicago, Illinois, USA, July 30 – August 3, 2005

scholarly journals Fibrillar biocomposite on the base of poly(3-hydroxybutyrate) nanofibers and iron oxide magnetic nanoparticles

2019 ◽  
Vol 489 (2) ◽  
pp. 157-160
Author(s):  
V. E. Prusakov ◽  
Yu. V. Maksimov ◽  
V. A. Beglov ◽  
M. V. Gerasimov ◽  
A. V. Bychkova ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Oxide Nanoparticles ◽  
The Novel ◽  
X Ray ◽  
X Ray Scattering ◽  
Transmission Electron ◽  
Iron Oxide Magnetic Nanoparticles

The novel biodegradable biocomposites on the base of poly(3-hydroxybutyrare) nanofibers with embedded iron oxide nanoparticles have been studied by transmission electron microscopy (TEM), Mssbayer spectroscopy, small angle X‑ray scattering (SAXS), and macroscopic magnetization techniques.

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