scholarly journals Deciphering microbiota associated to Rhynchophorus ferrugineus in Italian samples: a preliminary study

2012 ◽  
Vol 44 (3) ◽  
pp. 16 ◽  
Author(s):  
Matteo Valzano ◽  
Gabriele Achille ◽  
Francesca Burzacca ◽  
C. Damiani ◽  
Patrizia Scuppa ◽  
...  
Keyword(s):  
Control Method ◽  
Insect Pest ◽  
Bacterial Species ◽  
Coconut Oil ◽  
Pilot Project ◽  
Rhynchophorus Ferrugineus ◽  
Red Palm Weevil ◽  
Larval Stages ◽  
Palm Weevil ◽  
Preliminary Study

The Red Palm Weevil, <em>Rhynchophorus ferrugineus</em> (Olivier, 1790) is the most dangerous and deadly pest of date, coconut, oil, sago and other palms. Recently introduced in the Mediterranean basin, it became the most relevant insect pest for ornamental palms in the urban environment. Given the development of an innovative control method based on symbiotic control, we have performed a pilot project to decrypt the microbiota associated to both adults and larval stages of the insect to identify potential tools for biocontrol agents against the Palm Weevil. A number of bacterial species were found associated with the insect. In particular, species of the genera <em>Lactococcus</em>, <em>Proteus</em>, and others were detected<em>.</em>

scholarly journals Haplotype-resolved genome assembly enables gene discovery in the red palm weevil Rhynchophorus ferrugineus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Guilherme B. Dias ◽  
Musaad A. Altammami ◽  
Hamadttu A. F. El-Shafie ◽  
Fahad M. Alhoshani ◽  
Mohamed B. Al-Fageeh ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Control Strategies ◽  
Insect Pest ◽  
Single Copy ◽  
Gene Content ◽  
Rhynchophorus Ferrugineus ◽  
Red Palm Weevil ◽  
Transcriptomic Data ◽  
Palm Trees ◽  
Palm Weevil

AbstractThe red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) is an economically-important invasive species that attacks multiple species of palm trees around the world. A better understanding of gene content and function in R. ferrugineus has the potential to inform pest control strategies and thereby mitigate economic and biodiversity losses caused by this species. Using 10x Genomics linked-read sequencing, we produced a haplotype-resolved diploid genome assembly for R. ferrugineus from a single heterozygous individual with modest sequencing coverage ($$\sim$$ ∼ 62x). Benchmarking against conserved single-copy Arthropod orthologs suggests both pseudo-haplotypes in our R. ferrugineus genome assembly are highly complete with respect to gene content, and do not suffer from haplotype-induced duplication artifacts present in a recently published hybrid assembly for this species. Annotation of the larger pseudo-haplotype in our assembly provides evidence for 23,413 protein-coding loci in R. ferrugineus, including over 13,000 predicted proteins annotated with Gene Ontology terms and over 6000 loci independently supported by high-quality Iso-Seq transcriptomic data. Our assembly also includes 95% of R. ferrugineus chemosensory, detoxification and neuropeptide-related transcripts identified previously using RNA-seq transcriptomic data, and provides a platform for the molecular analysis of these and other functionally-relevant genes that can help guide management of this widespread insect pest.

scholarly journals Haplotype-resolved genome assembly enables gene discovery in the red palm weevil Rhynchophorus ferrugineus

2020 ◽  
Author(s):  
Guilherme B. Dias ◽  
Musaad A. Altammami ◽  
Hamadttu A.F. El-Shafie ◽  
Fahad M. Alhoshani ◽  
Mohamed B. Al-Fageeh ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Control Strategies ◽  
Insect Pest ◽  
Single Copy ◽  
Gene Content ◽  
Rhynchophorus Ferrugineus ◽  
Red Palm Weevil ◽  
Transcriptomic Data ◽  
Palm Trees ◽  
Palm Weevil

ABSTRACTThe red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) is an economically-important invasive species that attacks multiple species of palm trees around the world. A better understanding of gene content and function in R. ferrugineus has the potential to inform pest control strategies and thereby mitigate economic and biodiversity losses caused by this species. Using 10x Genomics linked-read sequencing, we produced a haplotype-resolved diploid genome assembly for R. ferrugineus from a single heterozygous individual with modest sequencing coverage (~62x). Benchmarking against conserved single-copy Arthropod orthologs suggests both pseudo-haplotypes in our R. ferrugineus genome assembly are highly complete with respect to gene content, and do not suffer from haplotype-induced duplication artifacts present in a recently published hybrid assembly for this species. Annotation of the larger pseudo-haplotype in our assembly provides evidence for 23,413 protein-coding loci in R. ferrugineus, including over 13,000 predicted proteins annotated with Gene Ontology terms and over 6,000 loci independently supported by high-quality Iso-Seq transcriptomic data. Our assembly also includes 95% of R. ferrugineus chemosensory, detoxification and neuropeptide-related transcripts identified previously using RNA-seq transcriptomic data, and provides a platform for the molecular analysis of these and other functionally-relevant genes that can help guide management of this widespread insect pest.

scholarly journals Pheromone receptor of the globally invasive quarantine pest of the palm tree, the red palm weevil (Rhynchophorus ferrugineus)

2020 ◽  
Author(s):  
Binu Antony ◽  
Jibin Johny ◽  
Nicolas Montagné ◽  
Emmanuelle Jacquin-Joly ◽  
Rémi Capoduro ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Insect Pest ◽  
Economic Losses ◽  
Palm Tree ◽  
Rhynchophorus Ferrugineus ◽  
Red Palm Weevil ◽  
Pheromone Receptor ◽  
Pheromone Detection ◽  
Palm Trees ◽  
Palm Weevil

AbstractPalm trees are of immense economic, sociocultural, touristic and patrimonial significance all over the world, and date palm-related knowledge, traditions and practices are now included in UNESCO’s list of the Intangible Cultural Heritage of Humanity. Of all the pests that infest these trees, the red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) is its primary enemy. The RPW is a category-1 quarantine insect pest that causes enormous economic losses in the cultivation of palm trees worldwide. The RPW synchronizes mass gathering on the palm tree for feeding and mating, regulated by a male-produced pheromone composed of two methyl-branched compounds, (4RS,5RS)-4-methylnonan-5-ol (ferrugineol) and 4(RS)-methylnonan-5-one (ferrugineone). Despite the importance of odorant detection in long-range orientation towards palm trees, palm colonization and mating, nothing regarding the molecular mechanisms of pheromone detection in this species is known. In this study, we report the identification and characterization of the first RPW pheromone receptor, RferOR1. Using gene silencing and functional expression in Drosophila olfactory receptor neurons, we demonstrate that RferOR1 is tuned to both ferrugineol and ferrugineone and binds five other structurally related molecules. We reveal the lifetime expression of RferOR1, which correlates with adult mating success irrespective of age, a factor that could explain the wide distribution and spread of this pest. As palm weevils are challenging to control based on conventional methods, elucidation of the mechanisms of pheromone detection opens new routes for mating disruption and the early detection of this pest via the development of pheromone receptor-based biosensors.

scholarly journals A QUARANTINE PROTOCOL AGAINST RED PALM WEEVIL RHYNCHOPHORUS FERRUGINEUS (OLIVIER) (COLEPTERA: CURCULIONDAE) IN DATE PALM

2013 ◽  
Vol 53 (4) ◽  
pp. 409-415 ◽  
Author(s):  
Abdul Moneim Al-Shawaf ◽  
Abdullah Al-Shagag ◽  
Mansour Al-Bagshi ◽  
Sami Al-Saroj ◽  
Salim Al-Bather ◽  
...  
Keyword(s):  
Middle East ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Rhynchophorus Ferrugineus ◽  
Red Palm Weevil ◽  
Partial Mortality ◽  
Larval Stages ◽  
Palm Weevil ◽  
The World ◽  
Better Than

Abstract The red palm weevil (RPW) Rhynchophorus ferrugineus (Olivier), (Coleptera: Curculiondae) is a key pest of date palm in the Middle East. This weevil is currently being reported from over 50% of the date growing countries of the world. The date palm Phoenix dactylifera cropped area in the Middle East, has significantly increased during the last two decades where date palm is mainly propagated through offshoots. The red palm weevil larvae are often found in the offshoots, resulting in the spread of the pest and also its re-inoculation where RPW is already controlled. Currently, there are no quarantine protocols to ensure that date palm offshoots which are transported for planting, are free of RPW larvae. In this study, date palm offshoots were sprayed while still attached to the mother palm and also dipped separately with Fipronil 3.5% (Thripguard 35 SCTM) and Imidacloprid 35% (Confidor 350 SCTM) a day after detachment from the mother palm. Fipronil and Imidacloprid were tested at 0.008 % and 0.01%, respectively. Results revealed that dipping gave complete mortality of the larvae. Dipping was significantly better than spraying since spraying resulted in only partial mortality of the larvae. It was also seen that dipping offshoots in Fipronil 0.004% and 0.002% for 30 and 60 min, respectively, resulted in 100% mortality of the larvae tested. Further, toxicity studies resulted in complete mortality of larvae and adults that were exposed to offshoot tissue that was dipped in Fipronil at the above concentrations. For this reason, it is recommended to dip date palm offshoots in 0.004% Fipronil for 30 min before transporting to ensure complete mortality of the hidden larval stages, if any and complete certification and transport of the treated offshoots to the new planting site within 72 h of treatment.

scholarly journals Electrocution and containment methods to reduce the activity of red palm weevil (Rhynchophorus ferrugineus, Ol.)

2020 ◽  
Vol 18 (4) ◽  
pp. e1006
Author(s):  
Marco Fedrizzi ◽  
Mauro Pagano ◽  
Mirko Guerrieri ◽  
Roberto Tomasone ◽  
Sergio Musmeci ◽  
...  
Keyword(s):  
Integrated Control ◽  
Plant Residues ◽  
Palm Tree ◽  
Strong Impact ◽  
Electric Discharges ◽  
Rhynchophorus Ferrugineus ◽  
Red Palm Weevil ◽  
Electric Voltage ◽  
Larval Stages ◽  
Palm Weevil

Aim of study: To evaluate the susceptibility of the Rhynchophorus ferrugineus to electric discharges, to eventually use this application in synergy with other methods as part of an integrated control strategy.Area of study: Worldwide.Material and methods: Three different electric voltage intensities were applied to R. ferrugineus to investigate the insect’s susceptibility in both adult and larval stages. The transmission and diffusion of electricity within a portion of the palm tree was tested. In addition, the suitability of containment materials for handling and transportation of plant residues was tested.Main results: The results of adult test showed that the total number of eggs laid per mating couple and the number of larvae born were about 1.5 times higher in the control compared to the electrified samples. The duration of the electric discharge on larvae had a small impact on the mortality rate, while the electric voltage showed a strong impact on mortality (87% for 10 V and 99% for 15 V). The presence of a significant electric current was observed in a viable portion of stem (distance from the source of electricity 10 cm) providing a direct current with 250 V tension. Among the materials tested for the transportation of palm residues, the aluminium net sheet showed the best results in the containment of both larval and adult individuals.Research highlights: Electrocution has proven to be a system which can help in the control of red palm weevil reducing the use of synthetic chemicals.

Quarantine Protocol Against Coleopteran Borers in Date Palm Offshoots Using Eco2 Fume Gas

2020 ◽  
Vol 31 (4) ◽  
pp. 190-192
Author(s):  
H.A. El-Shafie ◽  
M.E. Mohammed ◽  
A.A. Sallam
Keyword(s):  
Date Palm ◽  
Exposure Period ◽  
Rhynchophorus Ferrugineus ◽  
Internal Tissue ◽  
Red Palm Weevil ◽  
Rhinoceros Beetle ◽  
Planting Material ◽  
Palm Weevil ◽  
Longhorn Beetle ◽  
The World

Date palm offshoots represent an important source of planting material in many date palm-growing countries around the world. Infestation by the red palm weevil ((RPW) Rhynchophorus ferrugineus), the longhorn beetle ((LHB) Jebusaea hammerschmidti) and the rhinoceros beetle ((OB) Oryctes spp.) hinders commercialization and movement of these offshoots. An effective quarantine protocol, with exposure period of 72 h at 25 °C using ECO2FUME (EF) with phosphine concentration of 1500 ppm has been developed for date palm offshoots against these coleopteran internal tissue borers.

scholarly journals The Effect of Gut Bacteria on the Physiology of Red Palm Weevil, Rhynchophorus ferrugineus Olivier and Their Potential for the Control of This Pest

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 594
Author(s):  
Qian-Xia Liu ◽  
Zhi-Ping Su ◽  
Hui-Hui Liu ◽  
Sheng-Ping Lu ◽  
Bing Ma ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Current Knowledge ◽  
Management Strategies ◽  
Gut Bacteria ◽  
Economic Losses ◽  
Rhynchophorus Ferrugineus ◽  
Intestinal Immunity ◽  
Red Palm Weevil ◽  
Nutrient Metabolism ◽  
Palm Weevil

Red Palm Weevil (RPW), Rhynchophorus ferrugineus Olivier, is a notorious pest, which infests palm trees and has caused great economic losses worldwide. At present, insecticide applications are still the main way to control this pest. However, pesticide resistance has been detected in the field populations of RPW. Thus, future management strategies based on the novel association biological control need be developed. Recent studies have shown that the intestinal tract of RPW is often colonized by multiple microbial species as mammals and model insects, and gut bacteria have been found to promote the growth, development and immune activity of RPW larvae by modulating nutrient metabolism. Furthermore, two peptidoglycan recognition proteins (PGRPs), PGRP-LB and PGRP-S1, can act as the negative regulators to modulate the intestinal immunity to maintain the homeostasis of gut bacteria in RPW larvae. Here, we summarized the current knowledge on the gut bacterial composition of RPW and their impact on the physiological traits of RPW larvae. In contrast with metazoans, it is much easier to make genetic engineered microbes to produce some active molecules against pests. From this perspective, because of the profound effects of gut bacteria on host phenotypes, it is promising to dissect the molecular mechanisms behind their effect on host physiology and facilitate the development of microbial resource-based management methods for pest control.

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