Altering the Sex Pheromone Cyclo(l-Pro-l-Pro) of the Diatom Seminavis robusta towards a Chemical Probe

As a major group of algae, diatoms are responsible for a substantial part of the primary production on the planet. Pennate diatoms have a predominantly benthic lifestyle and are the most species-rich diatom group, with members of the raphid clades being motile and generally having heterothallic sexual reproduction. It was recently shown that the model species Seminavis robusta uses multiple sexual cues during mating, including cyclo(l-Pro-l-Pro) as an attraction pheromone. Elaboration of the pheromone-detection system is a key aspect in elucidating pennate diatom life-cycle regulation that could yield novel fundamental insights into diatom speciation. This study reports the synthesis and bio-evaluation of seven novel pheromone analogs containing small structural alterations to the cyclo(l-Pro-l-Pro) pheromone. Toxicity, attraction, and interference assays were applied to assess their potential activity as a pheromone. Most of our analogs show a moderate-to-good bioactivity and low-to-no phytotoxicity. The pheromone activity of azide- and diazirine-containing analogs was unaffected and induced a similar mating behavior as the natural pheromone. These results demonstrate that the introduction of confined structural modifications can be used to develop a chemical probe based on the diazirine- and/or azide-containing analogs to study the pheromone-detection system of S. robusta.

Correction: Sexual pheromone detection using PANI·Ag nanohybrid and PANI/PSS nanocomposite nanosensors

Correction for ‘Sexual pheromone detection using PANI·Ag nanohybrid and PANI/PSS nanocomposite nanosensors’ by Janine Martinazzo et al., Anal. Methods, 2021, 13, 3900–3908, DOI: 10.1039/d1ay00987g.

Sexual pheromone detection using PANI.Ag nanohybrid and PANI/PSS nanocomposite nanosensors

In this study, polyaniline/poly (styrene sulfonate) (PANI/PSS) nanocomposite and polyaniline.silver (PANI.Ag) nanohybrid thin films were obtained in cantilever nanosensors surface. The developed films were characterized in relation to topography, roughness,...

Pheromone receptor of the globally invasive quarantine pest of the palm tree, the red palm weevil (Rhynchophorus ferrugineus)

Palm trees are of immense economic, sociocultural, touristic and patrimonial significance all over the world, and date palm-related knowledge, traditions and practices are now included in UNESCO’s list of the Intangible Cultural Heritage of Humanity. Of all the pests that infest these trees, the red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) is its primary enemy. The RPW is a category-1 quarantine insect pest that causes enormous economic losses in the cultivation of palm trees worldwide. The RPW synchronizes mass gathering on the palm tree for feeding and mating, regulated by a male-produced pheromone composed of two methyl-branched compounds, (4RS,5RS)-4-methylnonan-5-ol (ferrugineol) and 4(RS)-methylnonan-5-one (ferrugineone). Despite the importance of odorant detection in long-range orientation towards palm trees, palm colonization and mating, nothing regarding the molecular mechanisms of pheromone detection in this species is known. In this study, we report the identification and characterization of the first RPW pheromone receptor, RferOR1. Using gene silencing and functional expression in Drosophila olfactory receptor neurons, we demonstrate that RferOR1 is tuned to both ferrugineol and ferrugineone and binds five other structurally related molecules. We reveal the lifetime expression of RferOR1, which correlates with adult mating success irrespective of age, a factor that could explain the wide distribution and spread of this pest. As palm weevils are challenging to control based on conventional methods, elucidation of the mechanisms of pheromone detection opens new routes for mating disruption and the early detection of this pest via the development of pheromone receptor-based biosensors.

Mechanisms of sensation

“Mechanisms of sensation” is the second chapter of the book Sensory Transduction and describes general features of sensory cells, including types of sensory membrane, the specialized organization of membrane and protein within sensory cells, membrane renewal, external specializations of sense cells, mechanisms of stimulus detection, primary and secondary receptor cells, and receptor sensitivity and noise. These general features of sensory cells are illustrated by specific examples taken from a wide variety of organisms, from scallop and crayfish to Drosophila and vertebrates including mammals. The chapter concludes with a description of sex pheromone detection in the male moth, which achieves the physical limit of sensitivity of the receptor to a single molecule of attractant.