scholarly journals Pleiotropy of chemically modified tetracycline in periodontitis

2013 ◽  
Vol 5 (3) ◽  
pp. 242 ◽  
Author(s):  
Ashish Agarwal
Keyword(s):  
Chemically Modified ◽  
Chemically Modified Tetracycline

The Effect of Chemically Modified Tetracycline-3 on the Progression of Dental Caries in Rats

2018 ◽  
Vol 52 (4) ◽  
pp. 297-302 ◽  
Author(s):  
Jun Xu ◽  
Congcong Miao ◽  
Zhenchuan Tian ◽  
Jinlu Li ◽  
Chunmei Zhang ◽  
...  
Keyword(s):  
Dental Caries ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Caries Prevalence ◽  
Chemically Modified ◽  
Dentin Caries ◽  
Chemically Modified Tetracycline

Objective: Matrix metalloproteinases (MMPs) exist in human saliva and dentin and play an important role in the degradation of organic matrix in teeth. Chemically modified tetracycline-3 (CMT-3) is an inhibitor of MMPs. CMT-3 has been used experimentally to treat caries since 1999, but no distinction between dental caries prevalence and dentin caries prevalence has been described. Methods: A total of 65 Sprague-Dawley rats were randomly divided into three groups. The positive control group (25 rats) was inoculated with Streptococcus mutans (ATCC700610) and fed the cariogenic feed of improved Keyes Diet 2000. The CMT-3 group (25 rats) was also inoculated with S. mutans and fed the cariogenic feed of improved Keyes Diet 2000; the surfaces of rats’ molars were daily treated with 0.02% CMT-3. The negative control group (15 rats) was only fed the standard rodent chow. At the end of the 10th week, the dental caries prevalence and dentin caries prevalence of each group were calculated, and the regions of caries were assessed. Results: No caries was found in the negative control group. The dental caries prevalence of the CMT-3 and the positive control group was 75.0 and 83.3%, respectively (p > 0.05, Table 2). The dentin caries prevalence of the CMT-3 and the positive control group was 33.3 and 70.8%, respectively (p < 0.05, Table 2). The Keyes scoring of dentin caries in the CMT-3 group was significantly lower than that in the positive control group (p < 0.05, Table 3). Conclusions: CMT-3 had no effect on the prevalence of dental caries, but could lower the prevalence and slow down the progression of dentin caries.

Chemically modified tetracycline-3 (CMT-3): A novel inhibitor of the serine proteinase, elastase

2011 ◽  
Vol 64 (6) ◽  
pp. 595-601 ◽  
Author(s):  
Ying Gu ◽  
Hsi-Ming Lee ◽  
Sanford R. Simon ◽  
Lorne M. Golub
Keyword(s):  
Serine Proteinase ◽  
Chemically Modified ◽  
Chemically Modified Tetracycline

Chemically-modified tetracycline normalizes collagen metabolism in diabetic rats: a dose-response study

1993 ◽  
Vol 28 (6) ◽  
pp. 420-428 ◽  
Author(s):  
Z. Yu ◽  
N. S. Ramamurthy ◽  
M. Leung ◽  
K. M. Chang ◽  
T. F. McNamara ◽  
...  
Keyword(s):  
Dose Response ◽  
Diabetic Rats ◽  
Collagen Metabolism ◽  
Chemically Modified ◽  
Chemically Modified Tetracycline ◽  
Dose Response Study

scholarly journals CHEMICALLY MODIFIED TETRACYCLINE (CMT) DECREASES NEUTROPHIL PROTEASE ACTIVITY AND PREVENTS POST-PUMP SYNDROME (PPS).

Shock ◽  
1998 ◽  
Vol 9 (Supplement) ◽  
pp. 33
Author(s):  
G Nieman ◽  
C Lutz ◽  
D Carney ◽  
A Picone ◽  
L Gatto ◽  
...  
Keyword(s):  
Protease Activity ◽  
Chemically Modified ◽  
Chemically Modified Tetracycline

ROLE OF CHEMICALLY MODIFIED TETRACYCLINE ON TNF-?? AND MITOGEN-ACTIVATED PROTEIN KINASES IN SEPSIS

Shock ◽  
2004 ◽  
Vol 22 (5) ◽  
pp. 478-481 ◽  
Author(s):  
Subir R Maitra ◽  
Sikha Bhaduri ◽  
Elliott Chen ◽  
Marc J Shapiro
Keyword(s):  
Protein Kinases ◽  
Mitogen Activated Protein Kinases ◽  
Chemically Modified ◽  
Chemically Modified Tetracycline ◽  
Mitogen Activated Protein

Novel mechanisms involving chemically modified tetracycline 3 cytotoxicity

2014 ◽  
Vol 25 (10) ◽  
pp. 1165-1174 ◽  
Author(s):  
Lin Zhao ◽  
Jiaying Xu ◽  
Yang Jiao ◽  
Haichao Wang ◽  
Saijun Fan
Keyword(s):  
Chemically Modified ◽  
Chemically Modified Tetracycline

Wound Healing in Ovariectomized Rats Effects of Chemically Modified Tetracycline (CMT-8) and Estrogen on Matrix Metalloproteinases -8, -13 and Type I Collagen Expression

2001 ◽  
Vol 8 (3) ◽  
pp. 281-294 ◽  
Author(s):  
Emma Pirila ◽  
Nungavarm Ramamurthy ◽  
Paivi Maisi ◽  
Steve McClain ◽  
Allan Kucine ◽  
...  
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinases ◽  
Type I Collagen ◽  
Ovariectomized Rats ◽  
Type I ◽  
Chemically Modified ◽  
Collagen Expression ◽  
Chemically Modified Tetracycline

Chemically Modified Tetracycline Improves Contractility in Porcine Coronary Ischemia/Reperfusion Injury

2006 ◽  
Vol 21 (3) ◽  
pp. 254-260 ◽  
Author(s):  
Michael F. Swartz ◽  
Jeffrey M. Halter ◽  
Gregory W. Fink ◽  
Lucio Pavone ◽  
Alexey Zaitsev ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Chemically Modified ◽  
Chemically Modified Tetracycline ◽  
Coronary Ischemia

scholarly journals CMT-308, a Nonantimicrobial Chemically-Modified Tetracycline, Exhibits Anti-Melanogenic Activity by Suppression of Melanosome Export

Biomedicines ◽  
2020 ◽  
Vol 8 (10) ◽  
pp. 411
Author(s):  
Shilpi Goenka ◽  
Sanford R. Simon
Keyword(s):  
Cell Cultures ◽  
Mammalian Cells ◽  
Selective Reduction ◽  
Mushroom Tyrosinase ◽  
Free System ◽  
Melanin Biosynthesis ◽  
Chemically Modified ◽  
B16f10 Cells ◽  
Chemically Modified Tetracycline

CMT-308 is a nonantimicrobial chemically-modified tetracycline (CMT), which we have previously shown exhibits antifungal activity and pleiotropic anti-inflammatory activities, including inhibition of the enzymatic activity of matrix metalloproteinases (MMPs). Based on its chemical structure, we hypothesized that CMT-308 could inhibit melanogenesis and might be a candidate for the treatment of skin hyperpigmentation disorders which occur due to unregulated melanin biosynthesis and/or transport. CMT-308 was first studied for any effects on activity of the enzyme tyrosinase in vitro using a purified preparation of mushroom tyrosinase; the mode of inhibition of the soluble fungal enzyme was evaluated by Lineweaver-Burk and Dixon plots as well as by non-linear least squares fitting. Next, the effects of CMT-308 were tested in mammalian cell cultures using B16F10 mouse melanoma cells and further validated in darkly-pigmented human melanocytes (HEMn-DP). Our results showed that micromolar concentrations of CMT-308 inhibited mushroom tyrosinase enzyme activity, using the first two substrates in the melanogenesis pathway (l-tyrosine and l-3,4-dihydroxyphenylalanine (l-DOPA)); CMT-308 inhibited mushroom tyrosinase primarily via a mixed mode of inhibition, with the major contribution from a competitive mode. In B16F10 cell cultures, CMT-308 (10 µM) significantly diminished total melanin levels with a selective reduction of extracellular melanin levels, under both basal and hormone-stimulated conditions without any cytotoxicity over a duration of 72 h. Studies of potential mechanisms of inhibition of melanogenesis in B16F10 cells showed that, in mammalian cells, CMT-308 did not inhibit intracellular tyrosinase activity or the activity of α-glucosidase, an enzyme that regulates maturation of tyrosinase. However, CMT-308 suppressed MITF protein expression in B16F10 cells and showed copper chelating activity and antioxidant activity in a cell-free system. The significantly lower extracellular melanin levels obtained at 10 µM indicate that CMT-308’s anti-melanogenic action may be attributed to a selective inhibition of melanosome export with the perinuclear aggregation of melanosomes, rather than a direct effect on the tyrosinase-catalyzed steps in melanin biosynthesis. These results were validated in HEMn-DP cells where CMT-308 suppressed dendricity in a fully reversible manner without affecting intracellular melanin synthesis. Furthermore, the capacity of CMT-308 to inhibit melanosome export was retained in cocultures of HEMn-DP and HaCaT. In summary, our results offer promise for therapeutic strategies to combat the effects of hyperpigmentation by use of CMT-308 at low micromolar concentrations.

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