Glutathione peroxidase activity in obese and nonobese diabetic patients and role of hyperglycemia in oxidative stress

Background: Cholestatic liver disease, a serious chronic condition that develops progressive hepatic degeneration through free radicals. Objective: The present study was designed to extract and identify two flavonoids in Phlomoides hyoscyamoides plant, native to Iran and evaluate the role of quercetin identified on the liver injury among bile ductligated rats. Methods: This study was conducted on 25 male Wistar rats within three groups of sham control, mere bile duct-ligated, and bile duct-ligated with quercetin. The bile duct-ligated animals received quercetin at a dose of 50 mg/kg/day for 10 days, followed by biochemical tests, oxidative stress markers, activity of antioxidant enzymes and hematoxylin and eosin staining. Molecular docking was used to explore the interactive behavior of quercetin with glutathione peroxidase. Results: According to analyses of the obtained extract, two main active ingredients of P. hyoscyamoides were rutin and quercetin. Bile duct-ligated group showed a significant liver necrosis, a clear increase in plasma and tissue oxidative stress parameters, and a decrease in glutathione peroxidase activity as compared to sham control group. Quercetin injection in bile duct-ligated rats resulted in significant decrease in hydroxyproline, protein carbonyl and histopathologic indexes and significant increase in glutathione peroxidase activity (P-value≤0.05). Based on the molecular docking, the quercetin was able to regulate the glutathione peroxidase activity. Conclusion: The quercetin acts as an enzyme inducer by renewing the glutathione peroxidase activity and inhibiting the oxidation of proteins and hence decreases the oxidative stress. These results could be a sign of confirming the positive role of quercetin in attenuating the liver damage and degeneration.

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Selenium levels and glutathione peroxidase activity in patients with ataxia-telangiectasia: association with oxidative stress and lipid status biomarkers

Orphanet Journal of Rare Diseases ◽

10.1186/s13023-021-01732-5 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Itana Gomes Alves Andrade ◽

Fabíola Isabel Suano-Souza ◽

Fernando Luiz Affonso Fonseca ◽

Carolina Sanchez Aranda Lago ◽

Roseli Oselka Saccardo Sarni

Keyword(s):

Oxidative Stress ◽

Glutathione Peroxidase ◽

Peroxidase Activity ◽

Oxidized Ldl ◽

Reference Value ◽

Glutathione Peroxidase Activity ◽

Oxidative Stress Biomarkers ◽

Apo B ◽

Lipid Status ◽

And Oxidative Stress

Abstract Introduction Ataxia-Telangiectasia (A-T) is a multi-system disorder that may be associated with endocrine changes, oxidative stress in addition to inflammation. Studies suggest that selenium is a trace element related to protection against damage caused by oxidative stress. Objective To describe the plasma levels of selenium and erythrocyte glutathione peroxidase activity in A-T patients and to relate them to oxidative stress and lipid status biomarkers. Methods This is a cross-sectional and controlled study evaluating 22 A-T patients (age median, 12.2 years old) matched by gender and age with 18 healthy controls. We evaluated: nutritional status, food intake, plasma selenium levels, erythrocyte glutathione peroxidase activity, lipid status, inflammation and oxidative stress biomarkers. Results Adequate levels of selenium were observed in 24/36 (66.7%) in this evaluated population. There was no statistically significant difference between the groups in selenium levels [47.6 μg/L (43.2–57.0) vs 54.6 (45.2–62.6) μg/dL, p = 0.242]. Nine of A-T patients (41%) had selenium levels below the reference value. The A-T group presented higher levels of LDL-c, non-HDL-c, oxidized LDL, Apo B, Apo-B/Apo-A-I1, LDL-c/HDL-c ratio, malondialdehyde [3.8 µg/L vs 2.8 µg/L, p = 0.029] and lower Apo-A-I1/HDL-c and glutathione peroxidase activity [7300 U/L vs 8686 U/L, p = 0.005]. Selenium levels were influenced, in both groups, independently, by the concentrations of oxidized LDL, malonaldehyde and non-HDL-c. The oxidized LDL (AUC = 0.849) and ALT (AUC = 0.854) were the variables that showed the greatest discriminatory power between groups. Conclusion In conclusion, we observed the presence of selenium below the reference value in nearly 40% and low GPx activity in A-T patients. There was a significant, inverse and independent association between selenium concentrations and oxidative stress biomarkers. Those data reinforce the importance of assessing the nutritional status of selenium in those patients.

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OXIDATIVE STRESS MARKERS IN THE LUNGS UNDER EXPERIMENTAL HYPEROXIA WITH INHALED LIPOSOMES BASED ON EGG LECITHIN

Biomeditsina ◽

10.33647/2074-5982-15-3-49-58 ◽

2019 ◽

pp. 49-58

Author(s):

I. L. Kotovich ◽

Zh. A. Rutkovskaya ◽

A. D. Taganovich

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Glutathione Peroxidase ◽

Peroxidase Activity ◽

Alpha Tocopherol ◽

Oxygen Species ◽

Glutathione Peroxidase Activity ◽

Diene Conjugates ◽

Hyperoxia Exposure ◽

Egg Lecithin

Oxidative stress is considered to be a factor leading to lung damage in premature infants. The aim of this study was to investigate the effect of inhaled antioxidants incorporated into egg lecithin liposomes on the indicators of oxidative stress in the lungs of newborn guinea pigs under experimental hyperoxia (3 days). Bronchoalveolar lavage fl uid (BALF) was used as a material for the study. Under hyperoxia exposure, inhalation of liposomes containing N-acetylcysteine and alpha-tocopherol contributed to the suppression of the reactive oxygen species production by cells, normalization of glutathione peroxidase activity and carbonyls content, while not affecting the level of diene conjugates in BALF. The introduction of retinoid-containing liposomes (retinol and retinoic acid) under hyperoxia was accompanied by normalization of glutathione peroxidase activity as well as the content of protein oxidation products in BALF, while the generation of reactive oxygen species remained enhanced, and the diene conjugates and thiobarbituric acid reactive products exceeded the levels in animals exposed to hyperoxia alone. Thus, the inhaled liposomes containing retinoids and egg lecithin exhibit not only anti-, but also a prooxidant effect in the lungs under hyperoxia exposure, unlike the liposomal forms of N-acetylcysteine and alpha-tocopherol.

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Oxidative stress assessment by glutathione peroxidase activity and glutathione levels in response to selenium supplementation in patients with Mucopolysaccharidosis I, II and VI

Genetics and Molecular Biology ◽

10.1590/1678-4685-gmb-2017-0334 ◽

2019 ◽

Vol 42 (1) ◽

pp. 1-8 ◽

Cited By ~ 7

Author(s):

José Araújo de Oliveira-Silva ◽

Joyce Umbelino Pinto Yamamoto ◽

Renata Bernardes de Oliveira ◽

Vaneisse Cristina Lima Monteiro ◽

Beatriz Jurkiewcz Frangipani ◽

...

Keyword(s):

Oxidative Stress ◽

Glutathione Peroxidase ◽

Peroxidase Activity ◽

Selenium Supplementation ◽

Glutathione Peroxidase Activity ◽

Stress Assessment ◽

Mucopolysaccharidosis I

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Stimulation of Glutathione Peroxidase Activity Decreases HIV Type 1 Activation after Oxidative Stress

AIDS Research and Human Retroviruses ◽

10.1089/aid.1994.10.1451 ◽

1994 ◽

Vol 10 (11) ◽

pp. 1451-1461 ◽

Cited By ~ 111

Author(s):

CHRISTINE SAPPEY ◽

SYLVIE LEGRAND-POELS ◽

MARTIN BEST-BELPOMME ◽

ALAIN FAVIER ◽

BERNARD RENTIER ◽

...

Keyword(s):

Oxidative Stress ◽

Glutathione Peroxidase ◽

Peroxidase Activity ◽

Glutathione Peroxidase Activity ◽

Hiv Type 1 ◽

Stimulation Of

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Glutathione Peroxidase Activity, Plasma Total Antioxidant Capacity, and Urinary F2- Isoprostanes as Markers of Oxidative Stress in Anemic Dogs

Journal of Veterinary Internal Medicine ◽

10.1111/jvim.14847 ◽

2017 ◽

Vol 31 (6) ◽

pp. 1700-1707 ◽

Cited By ~ 6

Author(s):

A. Kendall ◽

A. Woolcock ◽

A. Brooks ◽

G.E Moore

Keyword(s):

Oxidative Stress ◽

Glutathione Peroxidase ◽

Antioxidant Capacity ◽

Peroxidase Activity ◽

Total Antioxidant Capacity ◽

Glutathione Peroxidase Activity ◽

Total Antioxidant ◽

Markers Of Oxidative Stress

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Vitamin E protection of obesity-enhanced vascular calcification in uremic rats

AJP Renal Physiology ◽

10.1152/ajprenal.00355.2013 ◽

2014 ◽

Vol 306 (4) ◽

pp. F422-F429 ◽

Cited By ~ 15

Author(s):

A. Peralta-Ramírez ◽

A. Montes de Oca ◽

A. I. Raya ◽

C. Pineda ◽

I. López ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

Glutathione Peroxidase ◽

Peroxidase Activity ◽

Calcium Concentration ◽

Soft Tissues ◽

Zucker Rats ◽

Glutathione Peroxidase Activity ◽

Obese Rats

This study aimed to determine the extent of extraskeletal calcification in uremic Zucker rats, by comparing obese and lean phenotypes, and to evaluate the influence of vitamin E (VitE) on the development of calcifications in both uremic rats and human vascular smooth muscle cells (HVSMCs) cultured in vitro. Zucker rats of lean and obese phenotypes with normal renal function [control (C); C-lean and C-obese groups] and with uremia [5/6 nephrectomy (Nx); Nx-lean and Nx-obese groups] and uremic rats treated with VitE (Nx-lean + VitE and Nx-obese + VitE groups) were studied. Uremic groups were subjected to Nx, fed a 0.9% phosphorus diet, and treated with calcitriol (80 ng/kg ip). The aortic calcium concentration was significantly higher ( P < 0.05) in Nx-obese rats (10.0 ± 2.1 mg/g tissue) than in Nx-lean rats (3.6 ± 1.3 mg/g tissue). A decrease in plasma glutathione peroxidase activity was observed in Nx-obese rats compared with Nx-lean rats (217.2 ± 18.2 vs. 382.3 ± 15.5 nmol·min−1·ml−1, P < 0.05). Treatment with VitE restored glutathione peroxidase activity and reduced the aortic calcium concentration to 4.6 ± 1.3 mg/g tissue. The differences in mineral deposition between Nx-lean, Nx-obese, Nx-lean + VitE, and Nx-obese + VitE rats were also evidenced in other soft tissues. In HVSMCs incubated with high phosphate, VitE also prevented oxidative stress and reduced calcium content, bone alkaline phosphatase, and gene expression of core-binding factor-α1. In conclusion, uremic obese rats develop more severe calcifications than uremic lean rats and VitE reduces oxidative stress and vascular calcifications in both rats and cultures of HVSMCs.

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Haemoglobin Oxidation in Whole Blood Samples From Sheep in Relation to Glutathione Peroxidase Activity

Australian Journal of Biological Sciences ◽

10.1071/bi9790451 ◽

1979 ◽

Vol 32 (5) ◽

pp. 451 ◽

Cited By ~ 1

Author(s):

JF Wilkins

Keyword(s):

Glutathione Peroxidase ◽

Oxidative Damage ◽

Peroxidase Activity ◽

Whole Blood ◽

Alternative Assessment ◽

Selenium Status ◽

Glutathione Peroxidase Activity ◽

Blood Samples ◽

Whole Blood Samples

A high correlation was observed between HzOz-induced oxidation of haemoglobin and glutathione peroxidase activity in whole blood samples from sheep. A role of this enzyme in the prevention of oxidative damage to the erythrocyte and its contents has been previously demonstrated. The possibility of using haemoglobin oxidation in whole blood as an alternative assessment of glutathione peroxidase activity and hence of selenium status is proposed.

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