Effect of short-term gavage of ethanolic extract of cogon grass (Imperata cylindrica L) root on the ovarian activity and estrus behavior of female mice

Abstract Premature loss of ovarian activity before 40 years of age is known as primary ovarian insufficiency (POI) and occurs in ∼1% of women. A more subtle decline in ovarian activity, known as premature ovarian ageing (POA), occurs in ∼10% of women. Despite the high prevalence of POA, very little is known regarding its genetic causation. Senataxin (SETX) is an RNA/DNA helicase involved in repair of oxidative stress-induced DNA damage. Homozygous mutation of SETX leads to the neurodegenerative disorder, ataxia oculomotor apraxia type 2 (AOA2). There have been reports of POI in AOA2 females suggesting a link between SETX and ovarian ageing. Here, we studied female mice lacking either one (Setx+/−) or both (Setx−/−) copies of SETX over a 12- to 14-month period. We find that DNA damage is increased in oocytes from 8-month-old Setx+/− and Setx−/− females compared with Setx+/+ oocytes leading to a marked reduction in all classes of ovarian follicles at least 4 months earlier than typically occurs in female mice. Furthermore, during a 12-month long mating trial, Setx+/− and Setx−/− females produced significantly fewer pups than Setx+/+ females from 7 months of age onwards. These data show that SETX is critical for preventing POA in mice, likely by preserving DNA integrity in oocytes. Intriguingly, heterozygous Setx loss causes an equally severe impact on ovarian ageing as homozygous Setx loss. Because heterozygous SETX disruption is less likely to produce systemic effects, SETX compromise could underpin some cases of insidious POA.

Download Full-text

Influence of short-term energy supplementation on estrus, ovarian activity, and blood biochemistry in Ossimi ewes synchronized with fluorogestone acetate in the subtropics

Theriogenology ◽

10.1016/j.theriogenology.2016.09.027 ◽

2017 ◽

Vol 88 ◽

pp. 152-157 ◽

Cited By ~ 4

Author(s):

W. Senosy ◽

G.B. Mahmoud ◽

Sh.M. Abdel-Raheem

Keyword(s):

Blood Biochemistry ◽

Ovarian Activity ◽

Short Term ◽

Term Energy ◽

Energy Supplementation

Download Full-text

Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells

BioMed Research International ◽

10.1155/2017/9738640 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 12

Author(s):

Sylwia Ciesiółka ◽

Joanna Budna ◽

Karol Jopek ◽

Artur Bryja ◽

Wiesława Kranc ◽

...

Keyword(s):

Real Time ◽

Granulosa Cells ◽

Hormone Receptor ◽

Proliferative Activity ◽

Cell Transformation ◽

Luteinizing Hormone Receptor ◽

Luteal Cell ◽

Ovarian Activity ◽

Short Term

The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation and transformation of granulosa cells (GCs) during folliculogenesis, oogenesis, and oocyte maturation. Although the role of 17 beta-estradiol (E2) in ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, and GCs-luteal cells transformation requires further research. Therefore, the goal of this study was to assess the real-time proliferative activity of porcine GCs in vitro in relation to connexin (Cx), luteinizing hormone receptor (LHR), follicle stimulating hormone receptor (FSHR), and aromatase (CYP19A1) expression during short-term (168 h) primary culture. The cultured GCs were exposed to acute (at 96 h of culture) and/or prolonged (between 0 and 168 h of culture) administration of 1.8 and 3.6 μM E2. The relative abundance of Cx36, Cx37, Cx40, Cx43, LHR, FSHR, and CYP19A1 mRNA was measured. We conclude that the proliferation capability of GCs in vitro is substantially associated with expression of Cxs, LHR, FSHR, and CYP19A1. Furthermore, the GC-luteal cell transformation in vitro may be significantly accompanied by the proliferative activity of GCs in pigs.

Download Full-text

Effects of Portulaca oleracea ethanolic extract on reproductive system of aging female mice

International Journal of Reproductive BioMedicine ◽

10.29252/ijrm.14.3.205 ◽

2016 ◽

Vol 14 (3) ◽

pp. 205-212 ◽

Cited By ~ 8

Author(s):

Akram Ahangarpour ◽

Zohreh Lamoochi ◽

Hadi Fathi Moghaddam ◽

Seyed Mohamad Taghi Mansouri ◽

◽

...

Keyword(s):

Reproductive System ◽

Ethanolic Extract ◽

Portulaca Oleracea ◽

Female Mice

Download Full-text

Preclinical safety evaluation of the ethanolic extract from guavira fruits (Campomanesia pubescens (D.C.) O. BERG) in experimental models of acute and short-term toxicity in rats

Food and Chemical Toxicology ◽

10.1016/j.fct.2018.04.063 ◽

2018 ◽

Vol 118 ◽

pp. 1-12 ◽

Cited By ~ 5

Author(s):

Gustavo Roberto Villas Boas ◽

Ariany Carvalho dos Santos ◽

Roosevelt Isaias Carvalho Souza ◽

Flávio Henrique Souza de Araújo ◽

Giseli Karenina Traesel ◽

...

Keyword(s):

Safety Evaluation ◽

Ethanolic Extract ◽

Experimental Models ◽

Short Term ◽

Preclinical Safety

Download Full-text

Influence of Progesterone-Treatment Length and eCG Administration on Appearance of Estrus Behavior, Ovulatory Success and Fertility in Sheep

Animals ◽

10.3390/ani9010009 ◽

2018 ◽

Vol 9 (1) ◽

pp. 9 ◽

Cited By ~ 7

Author(s):

Paula Martinez-Ros ◽

Alejandro Rios-Abellan ◽

Antonio Gonzalez-Bulnes

Keyword(s):

Drug Release ◽

Chorionic Gonadotrophin ◽

Ovulation Rate ◽

The Other ◽

Short Term ◽

Treatment Length ◽

Progesterone Secretion ◽

Progesterone Treatment ◽

Estrus Behavior

The present study characterizes, for sheep, the occurrence and timing of the onset of estrus behavior and ovulation and the yields obtained (ovulation rate, progesterone secretion, and fertility) after Controlled Internal Drug Release (CIDR) insertion for five, six, seven, or fourteen days, with or without equine chorionic gonadotrophin (eCG) in ewes of the Segureña meat breed. All the treatments showed above 80% of females displaying estrus, but the onset of estrus was earlier and more synchronized when using eCG and, among groups with eCG, onset of estrus was earlier in the sheep treated for 14 days than in the short-term treatments (p < 0.05 for all). Administration of eCG after either short- or long-term treatments assured the occurrence of fertile ovulations in all the animals. Conversely, ovulatory success without eCG was found to be dependent on treatment length, with a high percentage of animals ovulating after five days of treatment (83.3%) and very low percentages after treatment for six or seven days (40% and 20%, respectively). Ovulation rate and progesterone secretion were similar among animals ovulating, but ovulation failures predetermined the fertility yields obtained in response to the treatments. Hence, the best results were found after treatment for 14 days plus eCG, and for 5 days without eCG (83.3 for both, p < 0.05 when compared to the other groups with different treatment lengths and with or without eCG).

Download Full-text