Dietary selenium supplementation, clarified egg yolk extender and slow cooling improve cryopreserved sperm characteristics of Saanen buck

Cryopreservation is one of the widely accepted and useful methods for preservation of gamete especially the spermatozoa. Experiments were conducted to develop the protocols for cryopreservation of sperm of Labeo rohita and to assess the effect of cryopreserved sperm on fertilization and hatching. Six extenders Alsevers solution, egg-yolk citrate, urea egg-yolk, Kurokura-2, Ma and Mb and five cryoprotectants ethanol, methanol, DMSO, DMA and glycerol were employed. Diluents were prepared by mixing the cryoprotectants at 10% concentration of the extenders (% v/v). Milt and diluents were mixed at a ratio of 1:9 for Alsevers solution and Kurokura-2; and 1:4 for urea egg-yolk, egg-yolk citrate, Ma and Mb. Egg-yolk citrate with 10% DMSO showed best performance producing 85 ± 1.6% post-thaw motility followed by 83±2% and 81±2.9% with Alsevers solution and urea egg-yolk respectively. Other extenders did not produce satisfactory results. Milt was diluted at six ratios (1:2, 1:4, 1:7, 1:9, 1:15 and 1:20) and 1:4 dilution showed the highest post-thaw motility for egg-yolk citrate and urea egg-yolk and 1:9 for Alsevers solution. Six cryoprotectant concentrations (5, 7, 10, 15, 20% and 30%) were investigated and 10% concentration produced the highest post-thaw motility. In breeding trials, sperm preserved with egg-yolk citrate plus DMSO as well as Alsevers solution plus DMSO fertilized eggs and produced hatchlings. The fertilization and hatching rates were 57 ± 7% and 46.5 ± 3.5% for egg-yolk citrate, and 33.5 ± 3.5% and 27±3% for Alsevers solution respectively. Fresh sperm yielded 76 ± 4% fertilization and 70.5 ± 5.5% hatching. The protocol developed through this study can be applied for long-term conservation of genetic materials of L. rohita and the cryopreserved sperm can be used in artificial breeding for generating new individuals.DOI: http://dx.doi.org/10.3329/pa.v22i1-2.16474 Progress. Agric. 22(1 & 2): 123-137, 2011

Download Full-text

Effect of chronic exposure to excess dietary copper and dietary selenium supplementation on liver specimens from rats

American Journal of Veterinary Research ◽

10.2460/ajvr.2001.62.1423 ◽

2001 ◽

Vol 62 (9) ◽

pp. 1423-1427 ◽

Cited By ~ 5

Author(s):

Enrique M. Aburto ◽

Alastair E. Cribb ◽

I. Carmen Fuentealba

Keyword(s):

Chronic Exposure ◽

Selenium Supplementation ◽

Dietary Copper ◽

Dietary Selenium

Download Full-text

Low-temperature storage of mammalian spermatozoa

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1957.0068 ◽

1957 ◽

Vol 147 (929) ◽

pp. 498-508 ◽

Cited By ~ 38

Keyword(s):

Critical Temperature ◽

Low Temperatures ◽

Freezing Point ◽

Egg Yolk ◽

Slow Cooling ◽

Temperature Range ◽

Low Temperature Storage ◽

The Media ◽

Extent Of Damage ◽

Mammalian Spermatozoa

Experiments in this and other countries on the preservation of spermatozoa at very low temperatures have shown that no mammalian spermatozoa so far examined survive freezing when they are cooled ultra-rapidly from temperatures above freezing point to temperatures of — 79° C or below. Slow cooling and the addition of glycerol to the media in which the spermatozoa are suspended, however, permits survival of the spermatozoa of many species. In different animals, there are marked variations in the resistance of their spermatozoa to freezing and the proportion of spermatozoa which can be revived from very low temperatures may be influenced both by the concentration of glycerol added to the semen and by the composition of the diluting fluid. In experiments with the spermatozoa of the bull, ram, stallion and boar it has been found that during slow cooling to — 79° C there is a critical temperature range between — 15 and — 25° C at which the greatest amount of damage occurs. The rate at which the capacity for motility of the spermatozoa is destroyed within this critical temperature range is considerably reduced by allowing the spermatozoa to stand at 2° C in contact with a medium containing egg yolk and glycerol for 18 h before freezing. The extent of damage in the critical temperature range may also be reduced by cooling the specimens at a rate of 0-25 to 0-5° per second between —15 and —25° C.

Download Full-text

Effects of Dietary Selenium Supplementation on Seminiferous Tubules and SelW, GPx4, LHCGR, and ACE Expression in Chicken Testis

Biological Trace Element Research ◽

10.1007/s12011-016-0646-y ◽

2016 ◽

Vol 173 (1) ◽

pp. 202-209 ◽

Cited By ~ 11

Author(s):

Ahmed Khalid ◽

Nagam Khudhair ◽

Huang He ◽

Zheng Peng ◽

Tian Yaguang ◽

...

Keyword(s):

Selenium Supplementation ◽

Seminiferous Tubules ◽

Dietary Selenium ◽

Chicken Testis

Download Full-text

THE EFFECT OF ADDED DIETARY SELENIUM ON THE SELENIUM CONTENT OF MILK, URINE AND FECES

Canadian Journal of Animal Science ◽

10.4141/cjas80-010 ◽

1980 ◽

Vol 60 (1) ◽

pp. 79-86 ◽

Cited By ~ 15

Author(s):

L. J. FISHER ◽

J. MONTEMURRO ◽

C. HOOGENDOORN

Keyword(s):

Dietary Intake ◽

Treatment Period ◽

Sodium Selenite ◽

Normal Values ◽

Fold Increase ◽

Selenium Supplementation ◽

Identical Twin ◽

Selenium Content ◽

Variable Response ◽

Dietary Selenium

Three sets of identical twin cows were used to quantitate the excretion of selenium in feces, urine and milk in response to increments of dietary selenium. The first trial consisted of three test periods, each 8 days long, separated by recovery periods of 5 days. A solution of sodium selenite was applied to the grain portion of the ration to provide 0, 6.0, 12.0, 24.0 and 48.0 or 100.0 mg of sodium selenite per day. Samples of milk, urine and feces were collected on the last 2 days of each treatment period and analyzed for selenium. There was a significant, positive linear (P < 0.05) response between excretion of selenium in feces and increments of dietary selenium. There was a marked but variable response in selenium content of urine to dietary intake but selenium levels in milk were not increased above those of control animals. In a second trial, sodium selenite was fed at the rate of 170 and 260 mg per day for 10 days. These theoretically toxic levels resulted in 10- and 40-fold increases in the selenium levels of feces and urine, respectively, but only a 3-fold increase in selenium content of milk. At these high levels of selenium supplementation the amounts of selenium in the kidney and liver were increased above the normal values. This study shows that selenium in the form of sodium selenite would have to be fed at levels toxic to the cow before there would be any measurable increase of selenium in milk.

Download Full-text