Cryopreservation of Sperm of <i>Labeo rohita</i> (Hamilton, 1822) and Its Use in Fertilization and Hatching of Eggs

Cryopreservation is one of the widely accepted and useful methods for preservation of gamete especially the spermatozoa. Experiments were conducted to develop the protocols for cryopreservation of sperm of Labeo rohita and to assess the effect of cryopreserved sperm on fertilization and hatching. Six extenders Alsevers solution, egg-yolk citrate, urea egg-yolk, Kurokura-2, Ma and Mb and five cryoprotectants ethanol, methanol, DMSO, DMA and glycerol were employed. Diluents were prepared by mixing the cryoprotectants at 10% concentration of the extenders (% v/v). Milt and diluents were mixed at a ratio of 1:9 for Alsevers solution and Kurokura-2; and 1:4 for urea egg-yolk, egg-yolk citrate, Ma and Mb. Egg-yolk citrate with 10% DMSO showed best performance producing 85 ± 1.6% post-thaw motility followed by 83±2% and 81±2.9% with Alsevers solution and urea egg-yolk respectively. Other extenders did not produce satisfactory results. Milt was diluted at six ratios (1:2, 1:4, 1:7, 1:9, 1:15 and 1:20) and 1:4 dilution showed the highest post-thaw motility for egg-yolk citrate and urea egg-yolk and 1:9 for Alsevers solution. Six cryoprotectant concentrations (5, 7, 10, 15, 20% and 30%) were investigated and 10% concentration produced the highest post-thaw motility. In breeding trials, sperm preserved with egg-yolk citrate plus DMSO as well as Alsevers solution plus DMSO fertilized eggs and produced hatchlings. The fertilization and hatching rates were 57 ± 7% and 46.5 ± 3.5% for egg-yolk citrate, and 33.5 ± 3.5% and 27±3% for Alsevers solution respectively. Fresh sperm yielded 76 ± 4% fertilization and 70.5 ± 5.5% hatching. The protocol developed through this study can be applied for long-term conservation of genetic materials of L. rohita and the cryopreserved sperm can be used in artificial breeding for generating new individuals.DOI: http://dx.doi.org/10.3329/pa.v22i1-2.16474 Progress. Agric. 22(1 & 2): 123-137, 2011

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How are Kawaii (Cute) Fictional Characters Used in Japanese Advertising

Journal of Media Research ◽

10.24193/jmr.39.4 ◽

2021 ◽

Vol 14 (1 (39)) ◽

pp. 55-78

Author(s):

Oana-Maria BÎRLEA

Keyword(s):

Egg Yolk ◽

Cultural Dimensions ◽

Fictional Characters ◽

Japanese Company ◽

The Public ◽

Different Types ◽

The Government ◽

Commercial Advertising

This article aims to explain the use and role of cute, adora- ble characters in Japanese advertising. Kawaii culture or the “culture of cute” has become known worldwide mainly because of Hello Kitty, the cat which led the “pink globalization”, as Yano (2013) states. In this paper we have attempted to reveal the symbolism of these apparent meaning- less cute signs and characters. Starting from Hofstede’s theory of cultural dimensions (2001, 2003, 2010), which shows how a society’s culture influ- ences the values of its members, we intended to show how are these kawaii characters used in Japanese advertising and how they fit cultural specif- ics. Used either in non-commercial, educational or commercial advertising, cute personae can make the target audience feel more comfortable, secure and cooperating (Murakami, 2005). In this paper we have discussed the role of three iconic characters: the emblematic Hello Kitty, Kumamon, the lovely bear created by the government of Kumamoto Prefecture (Japan) and Gudetama, a lazy egg yolk produced by the Japanese company Sanrio in 2014. The selected characters reflect different types and personalities and each of them serves a specific purpose, but via the analysis conveyed we conclude that perhaps their main aim is to persuade and create a long-term relationship with the public.

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Effect of Diafenthiuron exposure under short and long term experimental conditions on hematology, serum biochemical profile and elemental composition of a non-target organism, Labeo rohita

Environmental Toxicology and Pharmacology ◽

10.1016/j.etap.2018.06.006 ◽

2018 ◽

Vol 62 ◽

pp. 40-45 ◽

Cited By ~ 4

Author(s):

Muhammad Riaz-ul-Haq ◽

Rabia Javeed ◽

Saman Iram ◽

Muhammad Arslan Rasheed ◽

Muhammad Amjad ◽

...

Keyword(s):

Elemental Composition ◽

Labeo Rohita ◽

Biochemical Profile ◽

Experimental Conditions ◽

Serum Biochemical ◽

Short And Long Term ◽

Serum Biochemical Profile

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Intergenerational fluctuations in colour morph frequencies may maintain elytral polymorphisms in the ladybird beetle Cheilomenes sexmaculata (Coleoptera: Coccinellidae)

Biological Journal of the Linnean Society ◽

10.1093/biolinnean/blz031 ◽

2019 ◽

Vol 128 (3) ◽

pp. 725-734

Author(s):

Yasuko Kawakami ◽

Kazuo Yamazaki ◽

Kazunori Ohashi

Keyword(s):

Low Temperatures ◽

Life History Traits ◽

Environmental Heterogeneity ◽

Field Survey ◽

Hatching Rate ◽

Colour Morph ◽

Ladybird Beetle ◽

Term Field ◽

Hatching Rates

Abstract Phenotypic polymorphisms are found in a wide array of taxa, and unravelling the mechanisms that maintain them is of great interest to evolutionary and ecological biologists. Temporal environmental heterogeneity may play a role in the maintenance of polymorphisms but is poorly understood. In the present study, we analysed trends in intergenerational elytral colour morph frequencies in relation to changes in fitness and life history traits (i.e. body size, mortality, fecundity, hatching rate and mate preference) in the ladybird beetle Cheilomenes sexmaculata (Coleoptera: Coccinellidae). A long-term field survey spanning nine years showed that the frequency of dark morphs increases over winter and then decreases in spring. Dark morphs may have an advantage in winter due to their higher tolerance of low temperatures compared with light morphs. Light-morph females were heavier in winter than dark-morph females. They also mated more frequently and had higher hatching rates, potentially causing an increase in light morphs in spring. These results suggest that fluctuations in morph frequencies resulting from the conflicting directions of selection pressures between overwintering and spring generations may help to maintain genetic polymorphism.

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Cryopreservation of boar semen

Czech Journal of Animal Science ◽

10.17221/47/2020-cjas ◽

2020 ◽

Vol 65 (No. 4) ◽

pp. 115-123

Author(s):

Marija Jovičić ◽

Eva Chmelíková ◽

Markéta Sedmíková

Keyword(s):

Animal Species ◽

Semen Quality ◽

Egg Yolk ◽

High Rate ◽

Freezing And Thawing ◽

Long Term Storage ◽

Boar Semen ◽

Boar Spermatozoa ◽

Term Storage

Sperm cryopreservation is the best technology for long-term storage of the semen. However, the damage of boar spermatozoa by cryopreservation is more severe than in other animal species and a standardized freezing protocol for efficient cryopreservation has not been established yet. Semen quality and freezability vary greatly between breeds as well as between individual boars and even the season. Boar spermatozoa are sensitive to low temperatures; they sustain damage and a high rate of mortality and freezing/thawing the boar semen may strongly impair the sperm function and decrease the semen quality. The freezability of boar semen can be influenced by a cryopreservation procedure, and also by using various additives to freezing and thawing extenders such as antioxidants. In order to obtain acceptable results after thawing the boar semen, it is necessary to combine an optimal amount of additives (glycerol, egg yolk, sugars, antioxidants), cooling and warming velocities.

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Effect of Dimethyl sulfoxide (DMSO) and egg yolk on sperm motility, fertility and hatching rates of depik Rasbora tawarensis (Pisces: Cyprinidae) eggs after short‐term cryopreservation

Aquaculture Research ◽

10.1111/are.14516 ◽

2020 ◽

Vol 51 (4) ◽

pp. 1700-1705

Author(s):

Zainal A. Muchlisin ◽

Putri I. Sarah ◽

Dhea F. Aldila ◽

Kartini Eriani ◽

Iwan Hasri ◽

...

Keyword(s):

Dimethyl Sulfoxide ◽

Sperm Motility ◽

Egg Yolk ◽

Short Term ◽

Hatching Rates

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Vitrification Using Soy Lecithin and Sucrose: A New Way to Store the Sperm for the Preservation of Canine Reproductive Function

Animals ◽

10.3390/ani10040653 ◽

2020 ◽

Vol 10 (4) ◽

pp. 653

Author(s):

Maja Zakošek Pipan ◽

Margret L. Casal ◽

Nataša Šterbenc ◽

Irma Virant Klun ◽

Janko Mrkun

Keyword(s):

Semen Quality ◽

Reproductive Function ◽

Egg Yolk ◽

Final Concentration ◽

Freezing Process ◽

Soy Lecithin ◽

Group A ◽

Short And Long Term

A challenge in freezing semen for short and long-term availability is avoiding damage to intact spermatozoa caused by the freezing process. Vitrification protocols provide better results through less manipulation of semen and shorter freezing time compared to slow freezing techniques. Our research was aimed at improving vitrification methods for canine semen. Semen quality was determined in 20 ejaculates after collection. Each ejaculate was divided into eight aliquots, each with a different extender. The control extender contained TRIS, citric acid, fructose, and antibiotics. Soy lecithin and sucrose were added to the control extender at different concentrations to make up the test extenders and final concentration of 50 × 106 spermatozoa/mL. From each group, a 33 µL (1.65 × 106 spermatozoa) suspension of spermatozoa was dropped directly into liquid nitrogen and devitrified at least one week later and evaluated as before. Soy lecithin at 1% and 0.25 M sucrose added to the base vitrification media effectively preserved all sperm qualities. Our results demonstrate the effectiveness of our methods. Vitrification media containing sucrose and soy lecithin cause a minimal decline in quality of canine semen after devitrification. Furthermore, extenders used in our research did not contain egg yolk, which was replaced by soy lecithin, thus allowing for ease of shipping to other countries with strict requirements.

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Short- and long-term exposure to imidacloprid disturbs the elemental composition and free amino acid profile in muscles of Labeo rohita

Comparative Clinical Pathology ◽

10.1007/s00580-017-2538-8 ◽

2017 ◽

Vol 26 (6) ◽

pp. 1339-1346 ◽

Cited By ~ 1

Author(s):

Shazia Qadir ◽

Chen Xiwei ◽

Wei Wei ◽

Fengqin Feng ◽

Shahid Sultan ◽

...

Keyword(s):

Amino Acid ◽

Free Amino Acid ◽

Elemental Composition ◽

Free Amino ◽

Labeo Rohita ◽

Amino Acid Profile ◽

Short And Long Term

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Long-term egg-yolk adaptation of the Orientia tsutsugamushi for preparation of a formalinized immunogen

Vaccine ◽

10.1016/j.vaccine.2005.07.113 ◽

2006 ◽

Vol 24 (9) ◽

pp. 1438-1445 ◽

Cited By ~ 5

Author(s):

Y CHOI ◽

K KIM ◽

T KIM ◽

H CHEONG ◽

B AHN

Keyword(s):

Egg Yolk ◽

Orientia Tsutsugamushi

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ANALYSIS OF CRYOСONSERVED SPERM OF SIRES OF THE LEBEDINIAN BREED AND ORIGINAL BROWN CATTLE OF GERMANY

Animal Breeding and Genetics ◽

10.31073/abg.58.13 ◽

2019 ◽

Vol 58 ◽

pp. 95-101

Author(s):

V. I. Ladyka ◽

Y. I. Sklyarenko ◽

Y. M. Pavlenko ◽

O. V. Sherbak

Keyword(s):

Sperm Motility ◽

Sperm Production ◽

Long Term Storage ◽

Brown Cattle ◽

Cryopreserved Sperm ◽

Analysis System ◽

Quantitative Indicators ◽

Term Storage

In recent years, in Ukraine, as well as around the world, some local breeds have been on the verge of extinction, resulting in irretrievable loss of valuable genes, reduction of genetic diversity. In order to avoid these problems, it is necessary to work on the conservation of animal genetic resources. The creation of banks for long-term storage of biological material is one of the methods of preserving the gene pool of local, small and indigenous breeds of animals. Sperm quality is one of the main factors that determines the success of insemination of heifers and cows. Comprehensive analysis of sperm includes a large number of qualitative and quantitative indicators, the accuracy of which is influenced by a number of objective and subjective factors. Currently, microscopic analysis of sperm motility and survival is used in production conditions. Such approaches are easy to implement, but their results can be subjective. Research using a computer-based sperm fertility analysis system is becoming a priority today. With the help of CASA-Sperm Vision it is possible to carry out a morphological analysis already during the study of sperm motility. The purpose of our research was to analyze the quality of deep-frozen sperm of sires of Lebedinian breed and original brown cattle of Germany in order to attract such genetic material in the conservation of local Lebedinian breed cattle. Materials and methods. In the study used cryopreserved sperm doses of five sires of Lebedinian breed and three sires of the original brown cattle of Germany. Researches of qualitative, quantitative and dynamic characteristics of sperm of bulls were carried out at the laboratory of biotechnology of reproduction of Institute of Animal Breeding and Genetics nd. a. M.V.Zubets of NAAS and production laboratory "Ukrainian genetic company" using computer analysis of sperm motility Sperm Analysis System Version 12 IVOS. It was determined that the average dose of cryopreserved sperm of bulls of the Lebedinian breed was 0.25 ml, and of the sires of the original brown cattle of Germany -0.21 ml. It was found that the sires of the original brown cattle of Germany had the best indicators of total sperm motility by 21.7% compared to the sires of the Lebedinian breed (55.7%). They also noted a greater number of sperm with straight-forward movement of 16.5%. (40.2% of the Lebedinian breed sires). The average concentration of sperm in one milliliter was 10.5 times higher in animals of the Lebedinian breed (compared to the sires of the original brown cattle of Germany (331 million/ml). Comparing the indicators of the average speed of the sperm on the average trajectory (VAP), it should be noted that the minimum value was 85.3 microns/sec, the maximum value for this indicator – 141.7 microns/sec. The results of the VSL study showed that the average value of this indicator of sperm in the studied sperm doses of sires of the Lebedinian breed was 102 microns/sec, of the original brown cattle of Germany – 75. Obtained opposite to the direction coefficients of the correlation depending on the origin, between total sperm motility and average sperm speed for the average trajectory, the average speed of the sperm on the real trajectory, the average deviation of the sperm, the degree of straightness of the directed movement of sperm. Summary. 1. The complex of researches provided an objective analysis of qualitative and quantitative indicators of cryopreserved sperm production of the bulls of original brown cattle of Germany and the Lebedinian breed. It was found that the quality of the studied sperm doses met the requirements of "Instructions for Artificial Insemination of Cows and Heifers" (Order of the Ministry of Agrarian Policy of Ukraine dated August 1, 2001 № 230). 2. The comparative analysis of sperm production of the original brown cattle of Germany and the Lebedinian breed for its long-term storage was carried out. It is established that the sperm of sires of the original brown cattle of Germany exhibit higher dynamic characteristics of movement, while the sperm of sires of the Lebedinian breed were inferior to them in these indicators. 3. The possibility of insemination of females with cryopreserved sperm of the studied bulls whose sperm production has been stored for more than 30 years has been proved.

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