scholarly journals Effect of Tamoxifen and Raloxifene on the Proliferative Activity of the Breast Epithelium in Premenopausal Women

2015 ◽  
Vol 9 ◽  
pp. CMO.S22456 ◽  
Author(s):  
Miliana T. Lucato ◽  
Ruffo Freitas-Junior ◽  
Marise A. R. Moreira ◽  
Júlio R. M. Bernardes-Junior ◽  
Sebastião A. Pinto ◽  
...  
Keyword(s):  
Proliferative Activity ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Premenopausal Women ◽  
Normal Breast ◽  
Surgical Removal ◽  
Breast Epithelium ◽  
Normal Breast Epithelium ◽  
Ki 67 ◽  
Significant Difference

Objectives To compare the effects of tamoxifen and raloxifene on the proliferative activity of normal breast tissue in premenopausal women as measured by Ki-67/MIB-1 expression. Study Design A total of 48 women with benign breast nodules and a recommendation for surgical removal of the lesion took part in this study. They were randomized to use tamoxifen or raloxifene for 22 days, after which they were submitted to surgery. During the surgical procedure, a 1-cm fragment of normal breast tissue was removed to study Ki-67 expression. Results The mean percentage ratios between immunolabeled and non-labeled cells were 2.02 ± 1.09 and 3.13 ± 3.23 for the tamoxifen and raloxifene groups, respectively. There was no statistically significant difference between the tamoxifen ( n = 16) and raloxifene ( n = 14) groups in relation to the immunohistochemical analysis of Ki-67 ( P = 0.205). Conclusion The results of this study showed no difference between tamoxifen and raloxifene with respect to the potential of these drugs to reduce the proliferative activity of the normal breast epithelium in premenopausal women.

scholarly journals DNA Methylation and Breast Cancer Risk: An Epigenome-Wide Study of Normal Breast Tissue and Blood

Cancers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3088 ◽  
Author(s):  
Kaoutar Ennour-Idrissi ◽  
Dzevka Dragic ◽  
Elissar Issa ◽  
Annick Michaud ◽  
Sue-Ling Chang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Methylation ◽  
Breast Cancer Risk ◽  
Cancer Risk ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Breast Epithelium ◽  
Normal Breast Epithelium ◽  
Differentially Methylated Genes

Differential DNA methylation is a potential marker of breast cancer risk. Few studies have investigated DNA methylation changes in normal breast tissue and were largely confounded by cancer field effects. To detect methylation changes in normal breast epithelium that are causally associated with breast cancer occurrence, we used a nested case–control study design based on a prospective cohort of patients diagnosed with a primary invasive hormone receptor-positive breast cancer. Twenty patients diagnosed with a contralateral breast cancer (CBC) were matched (1:1) with 20 patients who did not develop a CBC on relevant risk factors. Differentially methylated Cytosine-phosphate-Guanines (CpGs) and regions in normal breast epithelium were identified using an epigenome-wide DNA methylation assay and robust linear regressions. Analyses were replicated in two independent sets of normal breast tissue and blood. We identified 7315 CpGs (FDR < 0.05), 52 passing strict Bonferroni correction (p < 1.22 × 10−7) and 43 mapping to known genes involved in metabolic diseases with significant enrichment (p < 0.01) of pathways involving fatty acids metabolic processes. Four differentially methylated genes were detected in both site-specific and regions analyses (LHX2, TFAP2B, JAKMIP1, SEPT9), and three genes overlapped all three datasets (POM121L2, KCNQ1, CLEC4C). Once validated, the seven differentially methylated genes distinguishing women who developed and who did not develop a sporadic breast cancer could be used to enhance breast cancer risk-stratification, and allow implementation of targeted screening and preventive strategies that would ultimately improve breast cancer prognosis.

Effects of low dose tamoxifen on normal breast tissue from premenopausal women

2003 ◽  
Vol 39 (7) ◽  
pp. 891-898 ◽  
Author(s):  
G.R. de Lima ◽  
G. Facina ◽  
J.Y. Shida ◽  
M.B.C. Chein ◽  
P. Tanaka ◽  
...  
Keyword(s):  
Breast Tissue ◽  
Low Dose ◽  
Normal Breast Tissue ◽  
Premenopausal Women ◽  
Normal Breast

Effects of raloxifene on normal breast tissue from premenopausal women

2005 ◽  
Vol 95 (2) ◽  
pp. 99-103 ◽  
Author(s):  
Benedito Borges da Silva ◽  
Ione Maria Ribeiro Soares Lopes ◽  
Luiz Henrique Gebrim
Keyword(s):  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Premenopausal Women ◽  
Normal Breast

scholarly journals THE ROLE OF METHYLATION OF A GENE GROUP’S ОF THE SHORT ARM OF CHROMOSOME 3 IN DIAGNOSTICS AND PROGNOSIS OF BIOLOGICAL SUBTYPES OF BREAST CANCER

2019 ◽  
Vol 65 (4) ◽  
pp. 556-564
Author(s):  
Denis Ryabchikov ◽  
Vitaliy Loginov ◽  
Tatyana Kazubskaya ◽  
Aleksey Burdennyy ◽  
Yelena Filippova ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Suppressor ◽  
Tumor Suppressor Genes ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Regulation Mechanism ◽  
Ki 67 ◽  
Suppressor Genes ◽  
Positive Ratio

Breast cancer (BC) is the multifactorial disease where different genes play the major pathogenic role with tumor-suppressor genes in particular. These genes methylation is supposed to be the common regulation mechanism. The aim of the present paper is to study methylation in group of tumor-suppressor genes: RASSF1A, SEMA3B, RARP2, RHOA, GPX1 and NKIRAS1 in luminal and mioepithelial breast cancer, and to evaluate their correlation with clinical subtype's course of this disease. Methods. Analysis of methylation was done in group of 174 malignant/normal breast tissue pairs and of 10 health breast tissue samples. Two independent methods a methyl-specific PCR (RASSF1A, RARP2, SEMA3B) and methylation-sensitive restriction analysis (RHOA, GPX1, NKIRAS were used. Results. A statistically significant high methylation frequency for genes RASSF1A, SEMA3B and RAR-p2 were shown (the positive ratio ranged from 13.2% to 37.9%) compared with normal breast tissue (the positive ratio ranged 0-6.9%, respectively), (p<0.00001). We revealed high frequent methylation level of RAR-P2 gene that was significantly higher in the luminal subtype B with the over expression of Her2 and Ki-67 compared with Her2-negative type and low level of Ki-67 (44% vs. 23.1%, p = 0.0458 and 38.5% vs. 16.2%, p = 0.0059, respectively). Interestingly we report that 5 and 10 years survivability without methylation was 93.5%, 85.5%, and 91.3%, 83.7%, respectively, compared ones with methylation where was lower 80.3 %, 65.3% (p = 0.007) and 84.3%, 67.2% (р=0,038) respectively. Conclusion. Methylation of RASSF1A, SEMA3B, RAR-P2, GPX1, RHOA и NKIRAS1 genes plays important role in tumorigenesis of luminal breast cancer by modulating their functions. Methylation of the RASSF1A and RAR-P2 genes is associated with an unfavorable the disease outcome. Identified epigenetic alterations in luminal subtypes BC could be included in the biomarker system to improve in evaluating diagnosis, prognosis of disease, especially for choosing the individual tactics of treatment.

Effect of a half dose of tamoxifen on proliferative activity in normal breast tissue

2000 ◽  
Vol 70 ◽  
pp. A86-A86
Author(s):  
J.R.M. Bernardes ◽  
M.T. Seixas ◽  
G.R. de Lima ◽  
E.C. Baracat ◽  
L.H. Gebrim
Keyword(s):  
Proliferative Activity ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Half Dose

Effect of a half dose of tamoxifen on proliferative activity in normal breast tissue

1999 ◽  
Vol 67 (1) ◽  
pp. 33-38 ◽  
Author(s):  
J.R.M. Bernardes ◽  
S. Nonogaki ◽  
M.T. Seixas ◽  
G. Rodrigues de Lima ◽  
E.C. Baracat ◽  
...  
Keyword(s):  
Proliferative Activity ◽  
Breast Tissue ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Half Dose
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