scholarly journals Lack of Association between Polymorphisms of Hepatic Lipase with Lipid Profile in Young Jordanian Adults

2014 ◽  
Vol 7 ◽  
pp. LPI.S14798
Author(s):  
Omar F. Khabour ◽  
Mahmoud A. Alomari ◽  
Karem H. Alzoubi ◽  
Mohammad Y. Gharaibeh ◽  
Farah H. Alhashimi
Keyword(s):  
Lipid Profile ◽  
Hepatic Lipase ◽  
Plasma Lipid ◽  
Lipoprotein Metabolism ◽  
Density Lipoprotein ◽  
Restriction Enzymes ◽  
Plasma Total Cholesterol ◽  
Chain Reaction ◽  
Males And Females ◽  
Polymerase Chain

The human hepatic lipase ( LIPC) gene encodes hepatic lipase, an enzyme involved in lipoprotein metabolism and regulation. Therefore, variants in LIPC gene may influence plasma lipoprotein levels. In this study, the association of LIPC C-514T and G-250A polymorphisms with plasma lipid profiles in 348 young Jordanians was investigated. Genotyping of C-514T and G-250A was performed by polymerase chain reaction and subsequent digestion with DraI and NiaIII restriction enzymes, respectively, while Roche analyzer was used to determine plasma total cholesterol, triglycerides, low-and high-density lipoprotein. The G-250 and C-514 alleles were most abundant in Jordanians with 79 and 80% frequencies, respectively. Additionally, no difference was found in the lipid–lipoprotein profile between the different genotype groups of C-514T or G-250A polymorphisms, even when males and females were examined separately ( P ≫ 0.05). In young Jordanian adults, the examined LIPC polymorphisms seem to play a limited role in determining the lipid profile.

Dyslipidemia of chronic renal failure: the nature, mechanisms, and potential consequences

2006 ◽  
Vol 290 (2) ◽  
pp. F262-F272 ◽  
Author(s):  
N. D. Vaziri
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Hepatic Lipase ◽  
Low Density Lipoprotein ◽  
Plasma Concentrations ◽  
Cholesterol Acyltransferase ◽  
Lipoprotein Metabolism ◽  
Density Lipoprotein ◽  
Triglyceride Rich Lipoprotein ◽  
Density Lipoprotein Receptor

Chronic renal failure (CRF) results in profound lipid disorders, which stem largely from dysregulation of high-density lipoprotein (HDL) and triglyceride-rich lipoprotein metabolism. Specifically, maturation of HDL is impaired and its composition is altered in CRF. In addition, clearance of triglyceride-rich lipoproteins and their atherogenic remnants is impaired, their composition is altered, and their plasma concentrations are elevated in CRF. Impaired maturation of HDL in CRF is primarily due to downregulation of lecithin-cholesterol acyltransferase (LCAT) and, to a lesser extent, increased plasma cholesteryl ester transfer protein (CETP). Triglyceride enrichment of HDL in CRF is primarily due to hepatic lipase deficiency and elevated CETP activity. The CRF-induced hypertriglyceridemia, abnormal composition, and impaired clearance of triglyceride-rich lipoproteins and their remnants are primarily due to downregulation of lipoprotein lipase, hepatic lipase, and the very-low-density lipoprotein receptor, as well as, upregulation of hepatic acyl-CoA cholesterol acyltransferase (ACAT). In addition, impaired HDL metabolism contributes to the disturbances of triglyceride-rich lipoprotein metabolism. These abnormalities are compounded by downregulation of apolipoproteins apoA-I, apoA-II, and apoC-II in CRF. Together, these abnormalities may contribute to the risk of arteriosclerotic cardiovascular disease and may adversely affect progression of renal disease and energy metabolism in CRF.

scholarly journals Plasma Lipid Profile of Moderate and Severe Hypertensive Patients

2020 ◽  
Vol 1 (4) ◽  
Author(s):  
Ibikunle Akinlua ◽  
Akinwumi T. Ogundajo ◽  
Olufisayo G. Oyebanji ◽  
Babajide O. Arobasalu
Keyword(s):  
Cardiovascular Diseases ◽  
Plasma Level ◽  
Lipid Profile ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
University Teaching ◽  
Lipoprotein Cholesterol ◽  
Hypertensive Patients ◽  
Control Subjects ◽  
Mild Hypertensive

Hypertension is the most prevalent and treatable risk factors for cardiovascular diseases and a major cause of morbidity and mortality worldwide. Elevated levels of lipid in the blood also known as hyperlipideamia or dyslipidemia have been implicated in the development of atherosclerosis and most cardiovascular diseases including hypertension. This study was designed to evaluate the plasma level of lipid profile in mild and severe hypertensive patients with a view to provide information on the link between these parameters and the development and severity of hypertension. Blood samples were collected from 120 freshly diagnosed hypertensive patients consisting of 60 mild and 60 severe hypertensive patients at Wesley Guide Hospital of Obafemi Awolowo University teaching Hospital Ilesa Osun state and 60 relatively healthy subjects as control. Plasma level of lipid profile [namely High density lipoprotein cholesterol (HDLc), low density lipoprotein cholesterol (LDLc), triglycerides (TG), total cholesterol (TC)] in the blood sample of both patients and control subjects were analyzed using standard methods. The results obtained were subjected to statistical analysis (p<0.05).The results of lipid profile in mild and severe hypertensive patients was compared to the control subjects. The results in mild hypertensive patients were also compared with the severe ones. There was a significant increase (P<0.05) in the plasma level of TC and LDLc in both mild and severe hypertensive patients when compared with the control subjects. Similarly a significant increase (p<0.05) was recorded in the plasma level of the both TC and LDLc in severe hypertensive patients when compared with mild hypertensive patients. However, the plasma level of HDLc in moderate and severe hypertensive patients was slightly lowered but not significant (p<0.05) while plasma TG level was not significantly different when compared with the control subjects. This study reveals a progressive increase in the plasma level of TC and LDLc from mild to severe hypertensive patients which could be a pointer to the fact that abnormalities in lipid metabolism might plays a significant role in the development and severity of hypertension.

scholarly journals Effect of Red Raspberry (Rubus idaeus L.) Consumption With or Without Fructo-Oligosaccharide on Metabolic Indices: A Single-Blinded Randomized, Crossover Clinical Trial (OR29-03-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Xuhuiqun Zhang ◽  
Anqi Zhao ◽  
Indika Edirisinghe ◽  
Amandeep Sandhu ◽  
Britt Burton-Freeman
Keyword(s):  
Clinical Trial ◽  
Lipid Profile ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
Rubus Idaeus ◽  
Lipoprotein Cholesterol ◽  
Lipid Lowering ◽  
Lowering Effect ◽  
Insulin Responses ◽  
Lipid Lowering Effect

Abstract Objectives Red raspberries (RRB) contain a unique polyphenol profile associated with cardio-metabolic benefits. Fructo-oligosaccharides (FOS), as prebiotics, present an approach to augment the cardio-metabolic benefits of RRB through their known effects in modifying the gut microbiota composition. The objective of this study is to determine the effect of RRB intake with or without FOS on glucose and insulin responses and fasting lipid profile in insulin-resistant (IR) (n = 20) and metabolically healthy reference (R) adults (n = 11) in a single-blinded, randomized, 12-week crossover clinical trial. Methods In this crossover study, after one-week run-in, subjects consumed RRB (1 cup RRB equivalence) or RRB + FOS (1 cup RRB equivalence with 8 g FOS) for 4 weeks in random order separated by 4-week washout between supplementation periods. Before and after each supplementation period, glucose and insulin responses were assessed by 2-h postprandial glycemic challenge with RRB (75 g glucose equivalence) and the plasma lipid profile characterized (total cholesterol (TC), high-density lipoprotein cholesterol (HDL), low-density lipoprotein cholesterol (LDL) and triglycerides (TG)). Results IR group had significantly elevated fasting and postprandial glucose and insulin, and higher fasting TG and lower HDL compared to R at baseline (0-week) (P ≤ 0.05); no differences in TC and LDL between groups (P > 0.05). After 4-week intervention, RRB decreased TC and LDL of IR group from baseline by 7% (P = 0.024) and 9% (P = 0.007), respectively, whereas adding FOS significantly attenuated the lipid-lowering effect of RRB. Alternatively, addingFOS to RRB augmented RRB effect on glycemic variables: 4-week intake of RRB + FOS significantly decreased 30 min incremental areas under curve (iAUC 0–30) of IR group for glucose and insulin from baseline by 20% (P = 0.014) and 18% (P = 0.012), respectively. Conclusions 4-week RRB intervention improves glycemic and lipid profiles in people with IR. Adding FOS to RRB supplement enhanced the glycemic benefits, but attenuated the lipid-lowering effect of RRB. Funding Sources This work was supported by the National Processed Raspberry Council.

scholarly journals Hepatic Lipase: a Comprehensive View of its Role on Plasma Lipid and Lipoprotein Metabolism

2015 ◽  
Vol 22 (10) ◽  
pp. 1001-1011 ◽  
Author(s):  
Junji Kobayashi ◽  
Kazuya Miyashita ◽  
Katsuyuki Nakajima ◽  
Hiroshi Mabuchi
Keyword(s):  
Hepatic Lipase ◽  
Plasma Lipid ◽  
Lipoprotein Metabolism ◽  
Comprehensive View ◽  
Lipase A

Determination of ABO glycosyltransferase genotypes by use of polymerase chain reaction and restriction enzymes

Transfusion ◽  
1995 ◽  
Vol 35 (3) ◽  
pp. 231-240 ◽  
Author(s):  
D. F. Stroncek ◽  
R. Konz ◽  
M. E. Clay ◽  
J. P. Houchins ◽  
J. McCullough
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Enzymes ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Plasma lipid profile of mice fed diet supplemented with broiler fat

2014 ◽  
Vol 43 (1) ◽  
pp. 21-24 ◽  
Author(s):  
GB Das ◽  
ME Hossain ◽  
MA Akbar
Keyword(s):  
Lipid Profile ◽  
Palm Oil ◽  
Low Density Lipoprotein ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
Dietary Treatment ◽  
Blood Lipid ◽  
Blood Cholesterol ◽  
Control Group ◽  
Blood Lipid Profile

This study was conducted with 180 female Swiss albino mice to examine the effects of supplementing broiler fat in the diets of mice on blood lipid profile. Twenty one days old mice were collected from ICDDRB, Mohakhali, Dhaka. They were randomly distributed in four groups having three replications each. Each replicate group had 15 mice. Results revealed that supplementation of broiler fat in mice diet significantly changed (p<0.05) triglyceride (TG) and low density lipoprotein (LDL) among groups. The control group (T0) had the highest level of cholesterol and LDL. Cholesterol, TG and LDL markedly increased (p<0.05) in all dietary treatment groups, while high density lipoprotein (HDL) significantly (p<0.01) decreased after supplementation of experimental diets. It was concluded that all the dietary treatments in general increased blood cholesterol, TG and LDL in mice fed broiler fat with different oil supplements. However, the soybean and palm oil group was comparatively better than other groups in respect of cholesterol, HDL and LDL at the blood lipid profile of mice. Therefore, it can be concluded that consumption of broiler fat with soybean and palm oil could be less harmful for mice.DOI: http://dx.doi.org/10.3329/bjas.v43i1.19380 Bang. J. Anim. Sci. 2014. 43 (1): 21-24

scholarly journals Specific Polymerase Chain Reaction Identification of Venturia nashicola Using Internally Transcribed Spacer Region in the Ribosomal DNA

2001 ◽  
Vol 91 (9) ◽  
pp. 900-904 ◽  
Author(s):  
Bruno Le Cam ◽  
Martine Devaux ◽  
Luciana Parisi
Keyword(s):  
Polymerase Chain Reaction ◽  
Ribosomal Dna ◽  
Related Species ◽  
Its Region ◽  
Restriction Enzymes ◽  
Oligonucleotide Primer ◽  
Sequence Information ◽  
Chain Reaction ◽  
Venturia Nashicola ◽  
Polymerase Chain

A technique based on the polymerase chain reaction (PCR) was developed for the identification of Venturia nashicola using nucleotide sequence information of the ribosomal DNA region. The complete internal transcribed spacer (ITS) region of V. nashicola strains and phylo-genetically related species was amplified with the two universal ITS1 and ITS4 primers, sequenced, and digested with five restriction enzymes. The alignment of nucleotide sequences and analyses of digestion patterns indicated constant polymorphisms between V. nashicola and related species at nucleotides 126 and 127, which overlapped a TaqI restriction site. An oligonucleotide primer named A126 was designed for identifying this variable region. A primer set (A126 and ITS4) that allowed the amplification of a 391-bp DNA fragment within the ITS region by PCR was specific to V. nashicola when it was checked against fungal genomic DNAs of related fungi. This primer set was a good candidate for a species-specific reagent in a procedure for identification of V. nashicola by PCR.

scholarly journals The lipoprotein/lipid profile is modulated by a gene–diet interaction effect between polymorphisms in the liver X receptor-α and dietary cholesterol intake in French-Canadians

2007 ◽  
Vol 97 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Julie Robitaille ◽  
Alain Houde ◽  
Simone Lemieux ◽  
Daniel Gaudet ◽  
Louis Pérusse ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Total Cholesterol ◽  
Ldl Cholesterol ◽  
Dietary Cholesterol ◽  
Plasma Lipid ◽  
Plasma Lipid Profile ◽  
Plasma Total Cholesterol ◽  
Lipoprotein Lipid ◽  
Liver X Receptor Α ◽  
Cholesterol Intake

Genetic and nutritional factors interact together and modulate the plasma lipid profile. We identified variations in the gene encoding the liver X receptor α (LXRα) and investigated their effects on the plasma lipoprotein/lipid profile. We also examined whether the association between cholesterol intake and plasma lipid profile was modulated by LXRα variants. The LXRα gene was sequenced in thirty-five French-Canadian men with high plasma total cholesterol (>5·0 mmol/l) and LDL-cholesterol (>3·5 mmol/l) concentrations. Dietary cholesterol was obtained from a food-frequency questionnaire. The LXRα c.-115G>A, c.-840C>A and c.-1830T>C genotypes were determined by direct sequencing in 732 subjects. Molecular screening of the LXRα gene revealed sixteen variants. Genotypes c.-115G>A, c.-840C>A and c.-1830T>C (rare allele frequency of 14·3 %, 14·2 % and 11·0 %, respectively) were analysed further. Plasma total cholesterol concentrations were higher in carriers of the -115A, -840A and -1830C allele, compared with the -115G/G, -840C/C and -1830T/T homozygotes (P ≤ 0·05). In a model including the c.-115G>A polymorphism, cholesterol intake, the interaction term c.-115G>A × cholesterol intake (mg/d) and covariates, LXRα-115G>A explained 1·8 % and 2·1 % of the variance in total cholesterol and LDL-cholesterol concentrations (P = 0·02 andP = 0·01), whereas the interaction term explained 2·9 % (P = 0·002) and 2·8 % (P = 0·005), respectively. When subjects were divided into four groups according to the median of cholesterol (290·8 mg) and -115G>A genotypes, high cholesterol intake was associated with higher cholesterol levels in -115A carriers. Similar results were observed for c.-840C>A and c.-1830T>C. These results suggest that cholesterol intake interacts with LXRα variants to modulate the plasma lipid profile.

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