Effects of source of rumen fluid on in vitro dry matter digestibility of feeds determined using the DAISYII incubator

2006 ◽  
Vol 86 (3) ◽  
pp. 439-441 ◽  
Author(s):  
J. King ◽  
J. C. Plaizier
Keyword(s):  
Key Words ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Ruminal Fluid ◽  
Total Mixed Ration ◽  
Inoculum Source ◽  
Dry Matter Digestibility ◽  
Percentage Points ◽  
Grass Hay

Apparent (ADD) and true (TDD) in vitro dry matter digestibilities of 12 ruminant feeds were determined with the DAISYII incubator (ANKOM Technology Corp. Macedon, NY) using inoculum prepared from ruminal fluid obtained from steers fed grass hay or from cows fed total mixed ration (56% forage and 44% grain, DM basis). Inoculum source did not affect ADD and TDD. Averaged across feeds and sources of inoculum, ADD was 6.7 percentage points lower than TDD. Key words: In vitro dry matter digestibility, ruminant feeds, inoculum, ANKOM DAISYII incubator

Comparisons of methods for in vitro dry matter digestibility of ruminant feeds

2005 ◽  
Vol 85 (2) ◽  
pp. 243-245 ◽  
Author(s):  
E. Brons ◽  
J. C. Plaizier
Keyword(s):  
Key Words ◽  
Dry Matter ◽  
Dry Matter Digestibility ◽  
Percentage Points ◽  
Grain Crop

Apparent in vitro dry matter digestibilities of selected ruminant feeds were determined with the DAISYII incubator (ADD, ANKOM Technology Corp., Macedon, NY) and the Tilley and Terry technique (ADTT). True in vitro dry matter digestibility was also determined with the DAISYII incubator (TDD). The ADD and ADTT did not differ for grain crop silages and total mixed rations. The ADD was 9.0 percentage points higher than ADTT for grains and 3.4 percentage points lower than ADTT for grass and legume forages. The TDD was between 5.7 and 11.2 percentage points higher than ADD depending on the feed. Key words: In vitro dry matter digestibility, forages, grains, ANKOM DAISYII incubator

In vitro degradation of fresh substrates treated with exogenous fibrolytic enzymes

2002 ◽  
Vol 82 (4) ◽  
pp. 611-615 ◽  
Author(s):  
G. R. Bowman ◽  
K. A. Beauchemin ◽  
J. A. Shelford
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Degradation ◽  
Total Mixed Ration ◽  
Fibrolytic Enzymes ◽  
Enzyme Product ◽  
Dry Matter Degradation

An exogenous fibrolytic enzyme product was applied to three different proportions of a total mixed ration (TMR) and fed to dairy cows. There was no enhancement of the hydrolytic capacity of rumen fluid from cows serving as inoculum donors as a result of enzyme supplementation. In vitro dry matter degradation of the TMR at 12 h of incubation was increased by 15% when enzymes were added to the concentrate portion, and by 17% when added to the premix portion of the TMR (P < 0.05), but adding enzymes to the supplement (pelleted portion of the concentrate) had no effect. Key words: Enzymes, fresh substrate, dry matter degradation

Differences between the in vitro digestibility of extrusa collected from oesophageal fistulated steers and the forage consumed

2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Pasture Legumes ◽  
Dry Matter Digestibility ◽  
Significant Difference ◽  
C3 Grasses

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.

scholarly journals Effects of Geraniol and Camphene on in Vitro Rumen Fermentation and Methane Production

2017 ◽  
Vol 48 (2) ◽  
pp. 63-69
Author(s):  
M. Joch ◽  
V. Kudrna ◽  
B. Hučko
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Positive Control ◽  
Rumen Fermentation ◽  
Dry Matter Digestibility

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.

EVALUATION OF THE TWO-STAGE TECHNIQUE FOR THE IN VITRO ESTIMATION OF THE DRY MATTER DIGESTIBILITY OF CORN SILAGE

1980 ◽  
Vol 60 (2) ◽  
pp. 367-378 ◽  
Author(s):  
R. J. BOILA ◽  
J. D. ERFLE ◽  
F. D. SAUER
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Corn Silage ◽  
Two Stage ◽  
Dry Matter Digestibility ◽  
Number Of Bacteria ◽  
Incubation Tube

The two-stage Tilley and Terry technique (incubation with rumen fluid followed by an acid-pepsin digest), used to estimate dry matter (DM) digestibility of forages in vitro, was evaluated with oven-dried corn silage as a substrate. The effect of weight of substrate (100–3000 mg), continuous shaking of incubations for the period of incubation with rumen fluid, number of bacteria present in the inoculum, and the contribution of bacterial dry matter to residual feed DM was measured. Percent dry matter digested decreased linearly as weight of substrate per incubation tube increased. Continuous shaking, as opposed to intermittent mixing (twice daily) during incubation with rumen fluid, increased the rate of DM disappearance and resulted in higher digestibility coefficients. Both the volume of inoculum and the number of bacteria present in that volume of inoculum influenced the percent DM digested. Bacteria contribute weight to residual feed DM unless steps are taken to remove them by centrifugation or solubilization.

scholarly journals Pengaruh Molases Pada Amoniasi Jerami Padi Menggunakan Urea Terhadap Kecernaan Bahan Kering dan Bahan Organik In Vitro

2008 ◽  
Vol 8 (2) ◽  
pp. 15-20 ◽  
Author(s):  
Muhamad Bata
Keyword(s):  
Organic Matter ◽  
Rice Straw ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Research Result ◽  
Optimal Level ◽  
Improve Quality ◽  
Dry Matter Digestibility ◽  
Organic Matter Digestibility

The effect of molasses on ammoniated straw by using urea on dry and organic matter digestibility as in vitroABSTRACT. Aimed of this research was to find out the optimal level of molasses addition to improve quality, dry matter and organic matter digestibility of rice straw ammonization process. Materials used were rumen fluid of fistula cattle, grind of rice straw, water, urea and molasses. Research designed used Completely Randomized Design (CRD). As treatments were R0: rice straw 1000 g dry matter + 500 g water + 50 g urea + 0 percent of molasses, R1: R0 + 15 percent of molasses, R2: R0 + 30 percent of molasses. Urea and molasses dissolved in water and then entered into pollybag. All pollybag observe and let for 15 days, each treatment replicated 6 times. Variable measured were dry matter digestibility and organic matter digestibility. Research result showed that ammonization product of NH3, Acidity Level and crude fiber having decreased while crude protein content increased. Variance analysis indicated that treatments had significant effect (P0.05) on dry matter and organic matter digestibility. Orthogonal polynomial test indicated that level of molasses increase (P0.05) of dry matter and organic matter digestibility linearly. It can be concluded that addition up to 30 percent in ammoniating of rice straw using urea can improve quality of ammonization and increasing dry matter and organic matter digestibility.

scholarly journals Influence of Rumen Fluid Source and Fermentation Time on In Vitro True Dry Matter Digestibility

1974 ◽  
Vol 57 (10) ◽  
pp. 1201-1205 ◽  
Author(s):  
R.J. Grant ◽  
P.J. Van Soest ◽  
R.E. McDowell
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Fermentation Time ◽  
Fluid Source ◽  
Dry Matter Digestibility
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