Comparisons of methods for in vitro dry matter digestibility of ruminant feeds

2005 ◽  
Vol 85 (2) ◽  
pp. 243-245 ◽  
Author(s):  
E. Brons ◽  
J. C. Plaizier
Keyword(s):  
Key Words ◽  
Dry Matter ◽  
Dry Matter Digestibility ◽  
Percentage Points ◽  
Grain Crop

Apparent in vitro dry matter digestibilities of selected ruminant feeds were determined with the DAISYII incubator (ADD, ANKOM Technology Corp., Macedon, NY) and the Tilley and Terry technique (ADTT). True in vitro dry matter digestibility was also determined with the DAISYII incubator (TDD). The ADD and ADTT did not differ for grain crop silages and total mixed rations. The ADD was 9.0 percentage points higher than ADTT for grains and 3.4 percentage points lower than ADTT for grass and legume forages. The TDD was between 5.7 and 11.2 percentage points higher than ADD depending on the feed. Key words: In vitro dry matter digestibility, forages, grains, ANKOM DAISYII incubator

Effects of source of rumen fluid on in vitro dry matter digestibility of feeds determined using the DAISYII incubator

2006 ◽  
Vol 86 (3) ◽  
pp. 439-441 ◽  
Author(s):  
J. King ◽  
J. C. Plaizier
Keyword(s):  
Key Words ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Ruminal Fluid ◽  
Total Mixed Ration ◽  
Inoculum Source ◽  
Dry Matter Digestibility ◽  
Percentage Points ◽  
Grass Hay

Apparent (ADD) and true (TDD) in vitro dry matter digestibilities of 12 ruminant feeds were determined with the DAISYII incubator (ANKOM Technology Corp. Macedon, NY) using inoculum prepared from ruminal fluid obtained from steers fed grass hay or from cows fed total mixed ration (56% forage and 44% grain, DM basis). Inoculum source did not affect ADD and TDD. Averaged across feeds and sources of inoculum, ADD was 6.7 percentage points lower than TDD. Key words: In vitro dry matter digestibility, ruminant feeds, inoculum, ANKOM DAISYII incubator

Short Communication: Prediction of in vitro dry matter digestibility with the ANKOM Daisy II system of ruminant feeds using the gas production technique

2013 ◽  
Vol 93 (3) ◽  
pp. 399-402
Author(s):  
J. C. Plaizier ◽  
S. Li
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Dry Matter ◽  
Short Communication ◽  
Prediction Equation ◽  
Gas Production ◽  
Production Technique ◽  
Dry Matter Digestibility ◽  
Grain Crop

Plaizier, J. C. and Li, S. 2013. Short Communication: Prediction of in vitro dry matter digestibility with the ANKOM Daisy II system of ruminant feeds using the gas production technique. Can. J. Anim. Sci. 93: 399–402. The potential of the gas production technique to predict in vitro dry matter digestibility was determined using 50 ruminant feeds, including grass/alfalfa forages, grain crop silages, grains, high protein feeds, and feed straws. Without including the chemical composition in the prediction equation, the prediction of IVDMD based on the cumulative gas production during 48 h alone was poor (R 2=0.48). Including the crude protein and neutral detergent fibre concentrations of the feed in the prediction equation improved the accuracy of the prediction (R 2=0.87), which was higher than that of predicting IVDMD based on the chemical composition alone (R 2=0.82).

A COMPARISON OF IN VITRO AND IN VIVO DRY MATTER DIGESTTBDLITY TECHNIQUES FOR THE EVALUATION OF FORAGE QUALITY

1987 ◽  
Vol 67 (2) ◽  
pp. 573-576 ◽  
Author(s):  
E. V. VALDES ◽  
G. E. JONES
Keyword(s):  
Key Words ◽  
Dry Matter ◽  
Forage Quality ◽  
In Vitro Digestibility ◽  
Two Stage ◽  
Dry Matter Digestibility ◽  
Whole Plant ◽  
Standard Deviations

A two-stage cellulase-pepsin technique (CS) was compared with the two-stage rumen inoculum-pepsin (IVDMD) method to predict in vivo dry matter digestibility (in vivo DMD) in grasses (n = 30) and legumes (n = 25). Comparisons of CS with IVDMD in whole-plant corn (n = 186) were also conducted. There were significant correlations (P < 0.01) between CS and in vivo DMD and between CS and IVDMD in all forages. The coefficients of determination (r2) and residual standard deviations for the prediction of IVDMD by CS varied between 0.76 and 0.90 and 2.5 and 3.4, respectively. Key words: Digestibility (in vitro), digestibility (in vivo), fungal cellulases, forage

The role of silicon in forages: A quantitative X-Ray study

1987 ◽  
Vol 45 ◽  
pp. 416-417
Author(s):  
Janet H. Woodward ◽  
D. E. Akin
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Acetate Buffer ◽  
Plant Tissues ◽  
Sodium Acetate Buffer ◽  
X Ray ◽  
Dry Matter Digestibility ◽  
Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

In Vivo Nylon Bag vs. Two‐Stage In Vitro Digestion: Comparison of Two Techniques for Estimating Dry‐Matter Digestibility of Forages 1

1969 ◽  
Vol 61 (4) ◽  
pp. 587-589 ◽  
Author(s):  
W. G. Manson ◽  
R. S. Lowrey ◽  
Ian Forbes
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Two Stage ◽  
Dry Matter Digestibility

Genetic Variability in In Vitro Dry Matter Digestibility of Fall‐Saved Reed Canarygrass, Phalaris arundinacea L. 1

Crop Science ◽  
1969 ◽  
Vol 9 (2) ◽  
pp. 162-164 ◽  
Author(s):  
I. T. Carlson ◽  
K. H. Asay ◽  
W. F. Wedin ◽  
R. L. Vetter
Keyword(s):  
Genetic Variability ◽  
Dry Matter ◽  
Phalaris Arundinacea ◽  
Reed Canarygrass ◽  
Dry Matter Digestibility

Divergent Selection for In Vitro Dry Matter Digestibility in Smooth Bromegrass 1

Crop Science ◽  
1986 ◽  
Vol 26 (6) ◽  
pp. 1123-1126 ◽  
Author(s):  
N. J. Ehlke ◽  
M. D. Casler ◽  
P. N. Drolsom ◽  
J. S. Shenk
Keyword(s):  
Dry Matter ◽  
Divergent Selection ◽  
Smooth Bromegrass ◽  
Dry Matter Digestibility ◽  
Selection For

scholarly journals Application of Selected Inoculant Producing Antifungal and Fibrinolytic Substances on Rye Silage with Different Wilting Time

Processes ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 879
Author(s):  
Seong-Shin Lee ◽  
Jeong-Seok Choi ◽  
Dimas Hand Vidya Paradhipta ◽  
Young-Ho Joo ◽  
Hyuk-Jun Lee ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Lactobacillus Brevis ◽  
Neutral Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Fiber Digestibility ◽  
Aerobic Stability ◽  
Commercial Inoculant

This research was conducted to determine the effects of selected inoculant on the silage with different wilting times. The ryes were unwilted or wilted for 12 h. Each rye forage was ensiled for 100 d in quadruplicate with commercial inoculant (Lactobacillus plantarum sp.; LPT) or selected inoculant (Lactobacillus brevis 100D8 and Leuconostoc holzapfelii 5H4 at 1:1 ratio; MIX). In vitro dry matter digestibility and in vitro neutral detergent fiber digestibility were highest in the unwilted MIX silages (p < 0.05), and the concentration of ruminal acetate was increased in MIX silages (p < 0.001; 61.4% vs. 60.3%) by the increase of neutral detergent fiber digestibility. The concentration of ruminal ammonia-N was increased in wilted silages (p < 0.001; 34.8% vs. 21.1%). The yeast count was lower in the MIX silages than in the LPT silages (p < 0.05) due to a higher concentration of acetate in MIX silages (p < 0.05). Aerobic stability was highest in the wilted MIX silages (p < 0.05). In conclusion, the MIX inoculation increased aerobic stability and improved fiber digestibility. As a result of the wilting process, ammonia-N in silage decreased but ruminal ammonia-N increased. Notably, the wilted silage with applied mixed inoculant had the highest aerobic stability.

scholarly journals 127 Porcine in vitro digestibility of whole stillage predigested with multi-enzyme during different time periods

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 49-50
Author(s):  
Kevin S Jerez Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Enzyme Treatment ◽  
Optimal Time ◽  
In Vitro Digestibility ◽  
Percentage Points ◽  
Whole Stillage ◽  
Freeze Dried ◽  
Different Sources

Abstract A study was conducted to determine the effects of the period of predigesting whole stillage (WS; slurry material that is dried into DDGS) with multi-enzyme and composition of the multi-enzyme on porcine in vitro digestibility of dry matter (IVDDM) of the WS. Four samples of whole stillage from 4 different sources were freeze-dried and divided into 13 subsamples to give 52 sub-samples. Thirteen treatments were applied to the 48 sub-samples within source. The treatments were undigested WS (control); or pre-digested with 1 of 3 multi-enzymes (MTE1, MTE2, and MTE3) at 55 °C for 6, 12, 18 or 24 h in 3 × 4 factorial arrangement. The MTE1 contained xylanase, β-glucanase, cellulase, mannanase, protease, and amylase; MTE2 contained xylanase, α-galactosidase, and cellulase; and MTE3 contained xylanase, cellulase, β-glucanase, and mannanase. The 52 subsamples were subjected to porcine in vitro digestion. The IVDDM of untreated WS was 73.3%. The IVDDM increased (P&lt; 0.05) with an increase in the predigestion period. However, a rise in the predigestion period from 0 to 12 h resulted in greater (P&lt; 0.05) response in mean IVDDM than an increment in the predigestion period from 12 to 24 h (11 vs. 0.83 percentage points). Predigestion period and multi-enzyme type interacted on IVDDM such that the improvement in IVDDM between 0 and 12 hours of predigestion differed (P&lt; 0.05) among the 3 multi-enzyme types (13.3, 11.1, and 8.5 percentage points for MTE3, MTE2, and MTE1, respectively). The LS means by multi-enzyme treatment were modeled and resulted in unparallel curves (P&lt; 0.05). The estimated maximum response of IVDDM for MTE1, MTE2 and MTE 3 were 82.4%, 84.7% and 87.1% at 15.8, 13 and 13.1 hours, respectively. In conclusion, the optimal time of predigestion of WS with multi-enzymes (with regard to improvement in its IVDDM) was approximately 14 h.

EFFECT OF STRUCTURAL AND CHEMICAL FACTORS OF FORAGES ON POTENTIALLY DIGESTIBLE FIBER, INTAKE, AND TRUE DIGESTIBILITY BY RUMINANTS

1988 ◽  
Vol 68 (3) ◽  
pp. 787-799 ◽  
Author(s):  
V. GIRARD ◽  
G. DUPUIS
Keyword(s):  
Cell Wall ◽  
Dry Matter ◽  
Plant Cell Wall ◽  
Neutral Detergent Fiber ◽  
Optimum Number ◽  
Apparent Digestibility ◽  
Cool Season ◽  
Dry Matter Digestibility ◽  
True Digestibility

In view of the large variation found in plant cell wall digestibilities with ruminants, an attempt was made to group 124 feeds into different lignification classes (clusters) on the basis of chemical characteristics. Each feed cluster was described using a structural coefficient [Formula: see text] that related the potentially digestible fiber (PDF, %) to the ratio between lignin and cell wall volume. The optimum number of clusters was determined iteratively by performing a regression of the apparent digestibility of dry matter at maintenance level (DDM1, %) against the PDF and cell soluble (SOL, %) contents of feeds. The [Formula: see text] coefficients varied from 0.05 (grains, N = 13) to 1.85 (corn silage, N = 3) and increased with the maturity of the grasses from 0.88 (legumes, vegetative cool season grasses, N = 26) to 1.33 (mature, cool season grasses, N = 19). Predicted PDF were closely correlated (r > 0.9, P < 0.01) to in vitro cell wall disappearances (IVCWD). Apparently digestible cell wall in four grasses and four legumes increased linearly with 96-h IVCWD and standard error (SE) was similar to the SE of predicted apparent digestible SOL from SOL concentrations. Assuming that similarity between SE could be also observed in larger samples, PDF and SOL were used in summative equations to predict apparent dry matter digestibility. DDM1 discounted for intake (DDM1 – 4, %) was regressed against SOL and PDF concentrations of 87 feeds:[Formula: see text]with ds and df, the true digestibilities of SOL and PDF. Estimates of ds and df were 0.98 and 0.95 for a zero-production (maintenance) level of intake, and 0.91 and 0.79 for an intake level four times maintenance. Since the true digestibility of the PDF component was only 4% – 13% lower than that of the cell soluble component, the concentration of PDF in cell wall was the major determinant in the variation in apparent digestibility of forages. Key words: lignin, neutral detergent fiber, true digestibility, cluster analysis, feeds

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