Influence of feeding a rapidly rumen degraded dietary protein at night or with a basal mixed ration on performance of dairy cows
Twelve multiparous and ten primiparous cows in late lactation were used in a 16-wk study to determine the influence of feeding times of a protein supplement (PS) on dry matter (DM) intake, ruminal fermentation, milk yield and milk composition. One mixed ration consisting of 50% whole crop oat silage, 10% alfalfa silage and 40% grain concentrate was offered twice daily with two-thirds at 08:00 h and the remainder at 18:00 h. In addition, a PS was offered in one meal per day at either 08:30 h or 00:30 h at an amount equal to 12% of the mixed ration DM intake. Half of the cows were assigned to each of the two dietary treatments which were designated as DAY (08:30 h) or NIGHT (00:30 h). Eight cows in the production study, previously fitted with large-diameter rumen cannula, were used to study treatment effects on rumen fermentation and ruminal in situ nutrient disappearance. Rate of passage of PS was measured using chromium-mordanted PS. Time of feeding PS had no effect on DM intake or body weight. Milk yield and milk composition were not influenced by treatment. Treatment had no effect on in situ DM and crude protein disappearance or ruminal rate of passage of PS. Concentrations of all VFA were not influenced by treatments. All measured volatile fatty acid concentrations were higher during the period of 01:00–06:00 h for cows fed the PS at night. Results indicate that timing of feeding a PS can alter ruminal fermentation characteristic without influencing animal performance. Key words: Protein supplement, feeding times, dairy cows, milk yield, rumen fermentation