Genetic diversification of the Chinese wheat landrace Mazhamai as revealed by morphological characteristics, seed storage proteins, and microsatellite markers

2007 ◽  
Vol 87 (4) ◽  
pp. 763-771 ◽  
Author(s):  
Lingli Zhang ◽  
Hongjie Li ◽  
Hui Wang ◽  
Lihui Li
Keyword(s):  
Microsatellite Markers ◽  
Storage Proteins ◽  
Genetic Relationships ◽  
Morphological Characteristics ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Geographical Regions ◽  
Wheat Landraces ◽  
Agronomical Traits

Chinese landraces of wheat (Triticum aestivum L. em Thell.) have had significant contributions not only to wheat production, but also to wheat improvement. Mazhamai is one of the most distinctive landraces, and has been used as a foundation parent in developing many outstanding improved cultivars in China. The objective of this study was to determine the genetic relationships of eight wheat landraces with the name Mazhamai, collected from wheat-producing regions with various geographical and microgeographic environments using morphological and agronomical traits, the composition of their gliadins and high-molecular-weight glutenin subunits (HMW-GS) and microsatellite markers. These homonymous accessions of Mazhamai differed, to some extent in their morphological and agronomical traits, gliadin composition, and microsatellite markers, but not for the HMW-GS and similar profiles for 52 of the SSR markers. This suggests that genetic variation must have occurred during the long-term growing of the Mazhamai accessions in various geographical regions. Furthermore, microsatellite analysis also revealed that the B genomewas more variable than the A and D genomes and chromosomes 2A and 2B were the most variable chromosomes among the Mazhamai accessions. However, chromosomes 1A, 1D, 2D, 5B, and 7A were conserved for the SSR loci used in the study. Key words: Wheat landraces, Mazhamai, genetic relationship, gliadin, glutenin, microsatellites

scholarly journals Genetic Diversity of Common Bean Genotypes as Revealed By Seed Storage Proteins and Some Agronomic Traits

2014 ◽  
Vol 67 (1) ◽  
pp. 125-137 ◽  
Author(s):  
Akbar Marzooghian ◽  
Mohammad Moghaddam ◽  
Mostafa Valizadeh ◽  
Mohammad Hasan Kooshki
Keyword(s):  
Genetic Diversity ◽  
Common Bean ◽  
Yield Components ◽  
Storage Proteins ◽  
Agronomic Traits ◽  
Morphological Characteristics ◽  
Seed Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Seed Morphology

AbstractEvaluation of the genetic diversity present within species is essential for conservation, management and utilization of the genetic resources. The objective of this study was to evaluate genetic variability of 70 common bean genotypes for seed storage proteins, grain morphological characteristics and agronomic traits. Two methods of extracting soluble seed proteins in salt were used.Positive correlations were observed among both seed morphological characters and developmental characters while yield components showed negative correlations with each other. Factor analysis for agronomic and grain morphological traits resulted in three factors were named yield components, seed morphology and phenology, respectively. Most genotypes had lower or medium scores for yield components and phenology factors. Considerable diversity was observed for seed morphology factor among the common bean genotypes.Nei’s diversity coefficient (He= 0.4), effective number of alleles (Ae= 1.69) and number of polymorphic loci (N = 17) indicated larger variation in the extraction method of soluble proteins in low salt (0.2 M NaCl) than high salt (1 M NaCl) condition. Considering that the centers of diversity for common bean are different in seed size, the result of Gst statistics showed that bands with relative mobility of 30, 32, 38 and 40 differentiated two weight groups more than other bands. Furthermore, significant differences were observed between these bands for number of pods per plant and number of seeds per plant.

Diversity of seed storage proteins in common wheat (Triticum aestivum L.)

2011 ◽  
Vol 9 (2) ◽  
pp. 256-259
Author(s):  
Zuzana Šramková ◽  
Edita Gregová ◽  
Svetlana Šliková ◽  
Ernest Šturdík
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Gel Electrophoresis ◽  
Storage Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Polyacrylamide Gel

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7+9 (43.3%) and 5+10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13+16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4+12, encoded by the Glu-D1 locus. The wheat–rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10.

Homology of chromosomes of Triticum macha Dek. et Men. and Triticum aestivum L. as shown with the help of genetic markers

Genome ◽  
1990 ◽  
Vol 33 (5) ◽  
pp. 755-757 ◽  
Author(s):  
E. V. Metakovsky ◽  
Z. A. Iakobashvili
Keyword(s):  
Triticum Aestivum ◽  
Genetic Analysis ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Triticum Aestivum L ◽  
Close Relationship ◽  
Homology Relationship ◽  
Relationship Of ◽  
Triticum Macha

Inheritance of the storage protein (gliadin and glutenin) genes of Triticum macha Dek. et Men. and their allelism to Triticum aestivum L. genes have been studied. A close homology of at least chromosomes 1A and 1B of the two species has been found. Results confirm a very close relationship between T. macha and T. aestivum.Key words: seed storage proteins, genetic analysis, chromosome homology, relationship of Triticum macha Dek. et Men. and Triticum aestivum L.

scholarly journals Genetic diversity of red clover cultivars (Trifolium pratense L.) based on protein polimorphism

Genetika ◽  
2010 ◽  
Vol 42 (2) ◽  
pp. 249-258 ◽  
Author(s):  
Zorica Nikolic ◽  
Sanja Vasiljevic ◽  
Djura Karagic ◽  
Milka Vujakovic ◽  
Dusica Jovicic ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Storage Proteins ◽  
Genetic Relationships ◽  
Geographic Origin ◽  
Seed Proteins ◽  
Red Clover ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Intellectual Property Right ◽  
Shikimate Dehydrogenase

Red clover is the second most important perennial forage legume. Based on morphological characters alone, it is difficult to distinguish accessories of red clover from each other because they have overlapping variations in terms of the major delimiting morphological and biological characters. The aim of this study was to analyze the genetic relationships of 32 red clover cultivars collected from European gene banks, as well as created in Serbia, based on seed storage proteins and isozymes. From 8 analyzed enzymic systems, there were no differences in zymograms for six enzymes only two enzymes were polymorphic. Two different allelic variants were found for enzyme shikimate dehydrogenase and three for phosphohexose isomeraze. The seed proteins in the area of higher molecular high weight, 55- 95 kDa, were identified as polimorphic. All the cultivars were placed into three clusters on the basis of Ward's distance range. The cluster pattern showed correlation between genetic diversity and geographic origin. Genetic distance between cultivars based on protein fingerprint could be used as a tool to control and protect intellectual property right over plant breeding material.

Methods of Identifying Genetic Diversity in Gliricidia Species for Biomass Production

1993 ◽  
Vol 29 (1) ◽  
pp. 87-96 ◽  
Author(s):  
J. R. Chamberlain ◽  
N. W. Galwey
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rural Communities ◽  
Storage Proteins ◽  
Morphological Characteristics ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Gliricidia Sepium ◽  
Morphological Characters ◽  
The Status

SummaryGliricidia sepium is a leguminous tree native to Mexico and Central America, and is utilized for fuelwood, animal fodder and green manure in rural communities. It is widely distributed throughout the tropics, but many populations of the tree have arisen from haphazard introductions of unknown quality. Methods for determining the genetic structure and origin of populations are therefore required as a basis for genetic improvement. In order to test the effectiveness of various measures of genetic diversity, provenances of G. sepium from Panama, Guatemala, Mexico and Honduras were studied by electrophoresis of seed storage proteins and the enzymes leucine aminopeptidase (LAP) and acid phosphatase (ACP), and by measurement of plant morphological characteristics. Study of seed storage proteins and morphological characters was very informative, and there was some genetic variation for ACP, but LAP showed no clear variation. In the cases where genetic variation was present, the different methods of study showed a consistent pattern of variation: genetic diversity was greatest in the population from Honduras, and least in that from Panama. This was contrary to expectations on the basis of the locations of origin of the populations and the range in which G. sepium is believed to be native. Electrophoresis of the storage proteins of Gliricidia maculata confirmed the status of this species as a distinct taxon.

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