scholarly journals Identification and Validation of a Microsatellite Marker for the Seedling Resistance Gene Lr24 in Bread Wheat

2015 ◽  
Vol 06 (06) ◽  
Author(s):  
Pallavi JK Anupam Singh
Keyword(s):  
Resistance Gene ◽  
Microsatellite Marker ◽  
Bread Wheat ◽  
Seedling Resistance ◽  
Seedling Resistance Gene

scholarly journals Lr72 Confers Resistance to Leaf Rust in Durum Wheat Cultivar Atil C2000

Plant Disease ◽  
2014 ◽  
Vol 98 (5) ◽  
pp. 631-635 ◽  
Author(s):  
S. A. Herrera-Foessel ◽  
J. Huerta-Espino ◽  
V. Calvo-Salazar ◽  
C. X. Lan ◽  
R. P. Singh
Keyword(s):  
Durum Wheat ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Triticum Turgidum ◽  
Single Gene ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Seedling Resistance ◽  
Seedling Resistance Gene

Leaf rust, caused by Puccinia triticina (Pt), has become a globally important disease for durum wheat (Triticum turgidum subsp. durum) since the detection of race group BBG/BN, which renders ineffective a widely deployed seedling resistance gene present in several popular cultivars including Mexican cultivars Altar C84 and Atil C2000. The resistance gene continues to play a key role in protecting durum wheat against bread wheat–predominant races since virulence among this race group has not been found. We developed F3 and F5 mapping populations from a cross between Atil C2000 and the susceptible line Atred #1. Resistance was characterized by greenhouse seedling tests using three Pt races. Segregation tests indicated the presence of a single gene, which was mapped to the distal end of 7BS by bulk segregant analysis. The closest marker, wmc606, was located 5.5 cM proximal to the gene. No known leaf rust resistance genes are reported in this region; this gene was therefore designated as Lr72. The presence of Lr72 was further investigated in greenhouse tests in a collection of durum wheat using 13 Pt races. It was concluded that at least one additional gene protects durum wheat from bread wheat–predominant Pt races.

Mining synthetic hexaploids for multiple disease resistance to improve bread wheat

2008 ◽  
Vol 59 (5) ◽  
pp. 421 ◽  
Author(s):  
F. C. Ogbonnaya ◽  
M. Imtiaz ◽  
H. S. Bariana ◽  
M. McLean ◽  
M. M. Shankar ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Stripe Rust ◽  
Stem Rust ◽  
Wheat Yield ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Seedling Resistance

A collection of 253 synthetic hexaploid wheats (SHWs) produced from 192 Aegilops tauschii accessions and 39 elite durum varieties were studied to identify, characterise, and evaluate potentially untapped diversity of disease resistance in wheat. The diseases for which resistance was sought included cereal cyst nematode (CCN), root lesion nematode (RLN), Stagonospora nodorum blotch (SNB), Septoria tritici blotch (STB), and the 3 rusts, leaf rust, stem rust, and stripe rust, all important diseases of bread wheat worldwide, which can severely reduce wheat yield and quality. The SHWs exhibited a wide spectrum of resistance to the 8 pathogens. The frequency of disease-resistant SHWs ranged from 1% for one species of RLN (Pratylenchus neglectus), 3% and 10% for Septoria nodorum leaf and glume blotch, 10% for seedling resistance to yellow leaf spot, 16% for CCN, 21% for the second species of RLN (Pratylenchus thornei), 73% for Septoria tritici blotch, and 15%, 40%, and 24% for leaf rust, stem rust, and stripe rust, respectively. Five SHWs, Aus26860, Aus30258, Aus30294, Aus30301, and Aus30304, exhibited high levels of resistance to CCN, YLP, STB, LR, and SR, while 56 SHWs showed resistance to either 3 or 4 diseases. The genetics of resistance to CCN in some of the SHWs revealed that some of the accessions carry the same CCN gene(s) against pathotype Ha13, while others may carry different resistance gene(s). Additional studies were carried out to understand the relationship between the resistances identified in SHWs and the ones already present in common wheat, in particular the resistance genes Cre1 and Cre3 against CCN. The use of perfect markers associated with Cre1 and Cre3 suggested that some SHWs may carry a new CCN resistance gene(s), which could be deployed in breeding programs to increase the diversity of available resistance. The identification of SHWs with resistance to a range of diseases provides an opportunity to generate genetic knowledge and resistant germplasm to be used in future variety development.

scholarly journals Genetic analysis of leaf rust resistance in three common wheat cultivars MP 3288, HI 784 and HI 1418 under field conditions

2019 ◽  
Vol 79 (01) ◽  
Author(s):  
T. L. Prakasha ◽  
S. Chand ◽  
A. N. Mishra ◽  
K. S. Solanki ◽  
J. B. Singh ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
The Other ◽  
Adult Plant ◽  
Wheat Cultivars ◽  
Seedling Resistance

This study aimed to investigate the genetic basis of leafrust resistance in three bread wheat cultivars viz., MP 3288, HI 1418 and HI 784 which have been maintaining high levels of resistance to leaf rust since their release in 2011, 2000, and 1983, respectively. These cultivars also possess leaf tip necrosis phenotype. These were crossed with a susceptible bread wheat cultivar Lal Bahadur and also among themselves in non-reciprocal manner.The F1 , F2 and F3 populations were raised and the inheritance of leaf rust resistance was studied using prevalent and highly virulent Puccinia triticina pathotype 77-5 (121R63-1) during 2014- 17. These studies showed that the field (adult-plant) resistance of these cultivars is governed by two dominant genes each. Closely linked molecular markers L34DINT9F and L34PLUSR revealed the presence of non-race specific adult-plant leaf rust resistance gene Lr34 in all cultivars of present study. Absence of the other documented race nonspecific APR genes viz., Lr46, Lr67 and Lr68 was indicated in all the three test cultivars based on genotyping with closely linked molecular markers WMC44, CFD71 and csgs, respectively. The other dominant gene appears to be an allstage resistance gene since all the three cultivars displayed high levels of seedling resistance to the test pathotype. Stable resistance of these cultivars could be due to synergistic/additive or complementary effects resulting from the combination of Lr34 and the all-stage resistance gene.

scholarly journals Map-Based Cloning of Leaf Rust Resistance Gene Lr21 From the Large and Polyploid Genome of Bread Wheat

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 655-664 ◽  
Author(s):  
Li Huang ◽  
Steven A Brooks ◽  
Wanlong Li ◽  
John P Fellers ◽  
Harold N Trick ◽  
...  
Keyword(s):  
Amino Acid ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Agronomic Traits ◽  
Leaf Rust Resistance ◽  
Triticum Aestivum L ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene

Abstract We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a generich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.

scholarly journals Diversity of Resistance Gene Analogues in Rust Resistance and Susceptible Bread Wheat Varieties

2019 ◽  
Vol 5 (2) ◽  
pp. 29-40
Author(s):  
Faranak Khanmakoo ◽  
Seyed Abolghasem Mohammadi ◽  
Robab Salami ◽  
Saeed Aharizad ◽  
◽  
...  
Keyword(s):  
Resistance Gene ◽  
Bread Wheat ◽  
Rust Resistance ◽  
Wheat Varieties

scholarly journals Inheritance and Molecular Mapping of Barley Genes Conferring Resistance to Wheat Stripe Rust

2005 ◽  
Vol 95 (8) ◽  
pp. 884-889 ◽  
Author(s):  
Vihanga Pahalawatta ◽  
Xianming Chen
Keyword(s):  
Resistance Gene ◽  
Microsatellite Marker ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Puccinia Striiformis ◽  
Infection Type ◽  
Dominant Gene ◽  
Wheat Stripe Rust ◽  
Rust Pathogen ◽  
Type Data

Most barley cultivars are resistant to stripe rust of wheat that is caused by Puccinia striiformis f. sp. tritici. The barley cv. Steptoe is susceptible to all identified races of P. striiformis f. sp. hordei (PSH), the barley stripe rust pathogen, but is resistant to most P. striiformis f. sp. tritici races. To determine inheritance of the Steptoe resistance to P. striiformis f. sp. tritici, a cross was made between Steptoe and Russell, a barley cultivar susceptible to some P. striiformis f. sp. tritici races and all tested P. striiformis f. sp. hordei races. Seedlings of parents and F1, BC1, F2, and F3 progeny from the barley cross were tested with P. striiformis f. sp. tritici races PST-41 and PST-45 under controlled greenhouse conditions. Genetic analyses of infection type data showed that Steptoe had one dominant gene and one recessive gene (provisionally designated as RpstS1 and rpstS2, respectively) for resistance to races PST-41 and PST-45. Genomic DNA was extracted from the parents and 150 F2 plants that were tested for rust reaction and grown for seed of F3 lines. The infection type data and polymorphic markers identified using the resistance gene analog polymorphism (RGAP) technique were analyzed with the Mapmaker computer program to map the resistance genes. The dominant resistance gene in Steptoe for resistance to P. striiformis f. sp. tritici races was mapped on barley chromosome 4H using a linked microsatellite marker, HVM68. A linkage group for the dominant gene was constructed with 12 RGAP markers and the microsatellite marker. The results show that resistance in barley to the wheat stripe rust pathogen is qualitatively inherited. These genes might provide useful resistance against wheat stripe rust when introgressed into wheat from barley.

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