scholarly journals Genetic Variation among Fragmented Populations of <i>Atriplex halimus</i> L. Using Start Codon Targeted (SCoT) and ITS1-5.8S-ITS2 Region Markers

2016 ◽  
Vol 06 (02) ◽  
pp. 101-115 ◽  
Author(s):  
Asmaa Elframawy ◽  
Hisham Deif ◽  
Ranya El-Bakatoushi
Keyword(s):  
Genetic Variation ◽  
Start Codon ◽  
Its2 Region ◽  
Atriplex Halimus ◽  
Fragmented Populations

Genetic variation within and among fragmented populations of lesser prairie-chickens (Tympanuchus pallidicinctus)

2003 ◽  
Vol 12 (3) ◽  
pp. 675-683 ◽  
Author(s):  
Ronald A. Van Den Bussche ◽  
Steven R. Hoofer ◽  
David A. Wiedenfeld ◽  
Donald H. Wolfe ◽  
Steve K. Sherrod
Keyword(s):  
Genetic Variation ◽  
Fragmented Populations ◽  
Tympanuchus Pallidicinctus

scholarly journals Population size, habitat fragmentation, and the nature of adaptive variation in a stream fish

2014 ◽  
Vol 281 (1790) ◽  
pp. 20140370 ◽  
Author(s):  
Dylan J. Fraser ◽  
Paul V. Debes ◽  
Louis Bernatchez ◽  
Jeffrey A. Hutchings
Keyword(s):  
Genetic Variation ◽  
Habitat Fragmentation ◽  
Population Size ◽  
Brook Trout ◽  
Small Populations ◽  
Adaptive Genetic Variation ◽  
Genetic Population ◽  
Fragmented Populations ◽  
Large Populations ◽  
Adaptive Differentiation

Whether and how habitat fragmentation and population size jointly affect adaptive genetic variation and adaptive population differentiation are largely unexplored. Owing to pronounced genetic drift, small, fragmented populations are thought to exhibit reduced adaptive genetic variation relative to large populations. Yet fragmentation is known to increase variability within and among habitats as population size decreases. Such variability might instead favour the maintenance of adaptive polymorphisms and/or generate more variability in adaptive differentiation at smaller population size. We investigated these alternative hypotheses by analysing coding-gene, single-nucleotide polymorphisms associated with different biological functions in fragmented brook trout populations of variable sizes. Putative adaptive differentiation was greater between small and large populations or among small populations than among large populations. These trends were stronger for genetic population size measures than demographic ones and were present despite pronounced drift in small populations. Our results suggest that fragmentation affects natural selection and that the changes elicited in the adaptive genetic composition and differentiation of fragmented populations vary with population size. By generating more variable evolutionary responses, the alteration of selective pressures during habitat fragmentation may affect future population persistence independently of, and perhaps long before, the effects of demographic and genetic stochasticity are manifest.

scholarly journals Comparison of Genetic Variation of Anthurium (Anthurium andraeanum) Cultivars Using SCoT, CDDP and RAPD Markers

2018 ◽  
Vol 28 (2) ◽  
pp. 171-182 ◽  
Author(s):  
Abbas Saidi ◽  
Zahra Daneshvar ◽  
Zohreh Hajibarat
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Variation ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Start Codon ◽  
Similarity Matrix ◽  
Random Amplification ◽  
Anthurium Andraeanum ◽  
Rapd Polymorphism

To evaluate the genetic diversity among 10 cultivars of anthurium were performed using three molecular markers such as Start Codon Targeted (SCoT) and Conserved DNA-derived Polymorphism (CDDP), and Random Amplification of Polymorphic DNA (RAPD). Polymorphism index content (PIC) was calculated 0.39, 0.42 and 0.37 for RAPD, SCoT and CDDP, respectively. This result showed all the three molecular markers had almost an identical potential in estimating genetic diversity. Cluster analysis using SCoT, CDDP and RAPD divided the cultivars to three distinct clusters. The similarity matrix obtained through SCoT and CDDP was positively significantly correlated (r = 0.76, p < 0.01). This is the first report in which the efficiency of two targeted DNA region molecular markers (SCoT and CDDP) together with RAPD technique have been compared with each other in a set of anthurium cultivras. Results suggested that SCOT, CDDP and RAPD fingerprinting techniques are of sufficient ability to detect polymorphism in anthurium cultivars. Plant Tissue Cult. & Biotech. 28(2): 171-182, 2018 (December)

Genetic variation between and within two populations of Rhabdocline pseudotsugae in Germany

2016 ◽  
Vol 46 (5) ◽  
pp. 716-724 ◽  
Author(s):  
Kristin Morgenstern ◽  
Jens-Ulrich Polster ◽  
Doris Krabel
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Spatial Distribution ◽  
Pseudotsuga Menziesii ◽  
Early Stage ◽  
Start Codon ◽  
Fruiting Bodies ◽  
Clear Differentiation ◽  
High Degree ◽  
Two Populations

Knowledge of the ecology and biology of Rhabdocline pseudotsugae Sydow is still at a very early stage. Recent results indicate the existence of an endophytic stage of the fungus in the form of symptomless infections in various Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) tissue types. This study represents the first description of genetic variation between and within two populations of R. pseudotsugae. Needles featuring fruiting bodies of R. pseudotsugae were collected from sample areas in Saxony and North Rhine-Westphalia (in Germany), with different fungal genotypes distinguished using the start codon targeted polymorphism (SCoT) and branch point signal sequences (BPS) techniques. Five of the 20 primers tested could be selected for further investigation. A total of 349 fragments were amplified at an average of 69.8 fragments per primer. Results showed the two sample areas to exhibit a high degree of both genetic variability and genetic diversity. A clear differentiation between the sample areas was not observed. There was nevertheless a clear connection between the genetic distance and spatial distribution of the fruiting bodies of R. pseudotsugae within the sample areas themselves.

High genetic variation in marginal fragmented populations at extreme climatic conditions of the Patagonian Cypress Austrocedrus chilensis

2010 ◽  
Vol 54 (3) ◽  
pp. 941-949 ◽  
Author(s):  
María Verónica Arana ◽  
Leonardo A. Gallo ◽  
Giovanni G. Vendramin ◽  
Mario J. Pastorino ◽  
Federico Sebastiani ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Climatic Conditions ◽  
Austrocedrus Chilensis ◽  
Fragmented Populations

scholarly journals Phylogenetic analysis of 23 accessions of Indonesian banana cultivars based on Internal Transcribed Spacer 2 (ITS2) region

2020 ◽  
Vol 25 (1) ◽  
pp. 1
Author(s):  
Karlia Meitha ◽  
Intan Fatmawati ◽  
Fenny Martha Dwivany ◽  
Agus Sutanto ◽  
Sigit Nur Pratama ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Internal Transcribed Spacer ◽  
Molecular Breeding ◽  
Future Research ◽  
Its2 Region ◽  
Phylogenetic Tree Analysis ◽  
Multiple Sequence ◽  
B Genome ◽  
A Genome

Pisang Kepok (Musa spp. [ABB ’Saba’ subgroup]) has several unique characteristics, such as tolerance to drought and Fusarium Foc (TR4) disease. Currently, the genetic diversity of Pisang Kepok in Indonesia is not well identified, although it is widely cultivated. Information on genetic diversity is essential for developing breeding strategies to achieve efficient cultivar improvement in the future. Aims of this research were to analyze the genetic variation of Pisang Kepok from some islands in Indonesia and to determine the genetic relationship between Pisang Kepok and other accessions banana cultivars based on ITS2 region, as a basis for future research in improving banana quality through molecular breeding. We have conducted the multiple sequence alignment and built the phylogenetic tree analysis using the Bayesian Inference Phylogeny method of one million generations (ngen = 1,000,000). The ITS2 region showed two clade ingroups: first clade consists of banana with B genome (balbisiana), while the second clade consists of banana with only A genome (acuminata). In general, all accessions of Pisang Kepok cultivars were clustered in the B genome of bananas cultivars. In addition, the ITS2 sequences and secondary structures among Pisang Kepok from various regions are identical, suggesting that there was no genetic variation in the ITS2 region of Pisang Kepok from multiple areas in Indonesia.

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