scholarly journals Nested polymerase chain reaction amplification and sequencing analysis of the light-chain and heavy-chain variable regions in the influenza A H1N1 virus hemagglutinin monoclonal antibody gene

2014 ◽  
Vol 13 (2) ◽  
pp. 4372-4379
Author(s):  
H.J. Li ◽  
C.Y. Guo ◽  
J.Y. Sun ◽  
L.J. Sun ◽  
P.H. Zhao ◽  
...  
2022 ◽  
Vol 6 (1) ◽  
pp. 1-6
Author(s):  
Kaziwa Ahmad Kaka alla ◽  
Salih Ahmed Hama

Influenza A (H1N1) virus is now rapidly scattering across the world. Early detection is one of the most effective measures to stop the further spread of the virus. The current study was aimed to detect influenza A (H1N1) serologically and by polymerase chain reaction (PCR) techniques. From September 2020 to June 2021, three hundred nasopharyngeal swabs and blood samples were collected from Hiwa and Shahid Tahir Hospitals in Sulaimani city. Obtained results revealed that 23.3% of the tested patients were seropositive anti-IgG for Influenza A, while 13.3% showed anti-IgM seropositive results although 10% of the tested cases were with both anti-IgG and anti-IgM seropositive results. Gender, residency, and flu symptoms showed no significant relations with seropositive results (p<0.05) whereas valuable relations were found between seropositive observations and smoking, the previous history of chronic diseases as well as employment status (p<0.05). It was concluded that hematologic investigations (CBC) were not dependable if H1N1 diagnosis and detection. Only 1% of the tested samples showed positive results for influenza A (H1N1) RNA using reverse transcription-PCR.


2012 ◽  
Vol 126 (12) ◽  
pp. 1274-1275 ◽  
Author(s):  
A A Alsanosi

AbstractObjective:We report deafness occurring as an extremely rare complication of influenza A caused by the H1N1 virus (‘swine flu’), in two children.Methods:Case reports and review of the literature concerning influenza A (H1N1) and acquired viral infection causing deafness.Results:Two children with normal hearing developed bilateral deafness following influenza A (H1N1). The diagnosis was confirmed using polymerase chain reaction. Both patients were treated with oseltamivir.Conclusion:Following a review of the literature, these two patients appear to be the first reported cases of bilateral deafness following influenza A (H1N1).


2016 ◽  
Vol 2016 ◽  
pp. 1-3 ◽  
Author(s):  
Can Hüzmeli ◽  
Mustafa Saglam ◽  
Ali Arıkan ◽  
Barıs Doner ◽  
Gulay Akıncı ◽  
...  

Influenza viruses are members of the Orthomyxoviridae family, of which influenza A, B, and C viruses constitute three separate genera. Arterial thrombosis associated with H1N1 influenza A virus infection has rarely been reported. A Turkish man aged 28 years was admitted to our emergency department with dyspnea, bilateral lower extremity insensitivity, and cold. He reported symptoms of fever, myalgia, and cough, which he had had for fifteen days before being admitted to our hospital. The patient was tested for pandemic influenza A (H1N1) virus using polymerase chain reaction (PCR) tests, which were positive. Abdominal computerized tomography with contrast revealed a large occlusive thrombus within the infrarenal aorta.


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