Pollen morphological characteristics, viability test and storage of endangered medicinal plant Atractylodes lancea

Hypericum scruglii Bacch., Brullo & Salmeri is an endangered endemic plant of Sardinia, and the phloroglucinol compounds identified in this species have been reported to inhibit the activity of the Human Immunodeficiency Virus. Seed banks are a genetic repository that effectively preserve taxa of conservation interest, and they hold knowledge about the biology and germination ecophysiology of the taxa they preserve. The main goals of this study were to investigate the germination requirements for H. scruglii, to evaluate the seed viability after eight years of long-term conservation and to suggest an efficient protocol for germination. Seeds stored at –25 °C in the seed bank were tested at temperatures from 5 to 30 °C and 25/10 °C. Base temperature (Tb) and thermal time (θ50) for germination were estimated. Seeds of H. scruglii germinated over a wide range of temperatures, responding positively to high temperatures and 25/10 °C. Tb was estimated at 4.92 °C, and θ50 was estimated at 198.27 degree days. The high viability detected in seeds of H. scruglii allows us to suggest the use of seeds stored in a seed bank when fresh material for plant propagation is scarce. Our results provide new and useful baseline information for implementing conservation and multiplication strategies for this endangered medicinal plant.

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Efficient plant regeneration through callus in Zanthoxylum armatum DC: an endangered medicinal plant of the Indian Himalayan region

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology ◽

10.1080/11263504.2019.1610107 ◽

2019 ◽

Vol 154 (3) ◽

pp. 288-294 ◽

Cited By ~ 6

Author(s):

Sumit Purohit ◽

Kuldeep Joshi ◽

Vivek Rawat ◽

Indra D. Bhatt ◽

Shyamal K. Nandi

Keyword(s):

Plant Regeneration ◽

Medicinal Plant ◽

Himalayan Region ◽

Indian Himalayan Region ◽

Zanthoxylum Armatum ◽

Endangered Medicinal Plant

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Endangered Uyghur Medicinal PlantFerulaIdentification through the Second Internal Transcribed Spacer

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/479879 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Congzhao Fan ◽

Xiaojin Li ◽

Jun Zhu ◽

Jingyuan Song ◽

Hui Yao

Keyword(s):

Medicinal Plant ◽

Related Species ◽

Internal Transcribed Spacer ◽

Morphological Characteristics ◽

Its2 Sequence ◽

Sequence Length ◽

Local Alignment ◽

Accurate Identification ◽

Distance Method ◽

Closely Related Species

The medicinal plantFerulahas been widely used in Asian medicine, especially in Uyghur medicine in Xinjiang, China. Given that various substitutes and closely related species have similar morphological characteristics,Ferulais difficult to distinguish based on morphology alone, thereby causing confusion and threatening the safe use ofFerula. In this study, internal transcribed spacer 2 (ITS2) sequences were analyzed and assessed for the accurate identification of two salableFerulaspecies (Ferula sinkiangensisandFerula fukangensis) and eight substitutes or closely related species. Results showed that the sequence length of ITS2 ranged from 451 bp to 45 bp, whereas guanine and cytosine contents (GC) were from 53.6% to 56.2%. A total of 77 variation sites were detected, including 63 base mutations and 14 insertion/deletion mutations. The ITS2 sequence correctly identified 100% of the samples at the species level using the basic local alignment search tool 1 and nearest-distance method. Furthermore, neighbor-joining tree successfully identified the genuine plantsF. sinkiangensisandF. fukangensisfrom their succedaneum and closely related species. These results indicated that ITS2 sequence could be used as a valuable barcode to distinguish Uyghur medicineFerulafrom counterfeits and closely related species. This study may broaden DNA barcoding application in the Uyghur medicinal plant field.

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