scholarly journals Relationship Between Immunohistochemical Expression Level of CD47 and Pathological Disease Stage in Prostate Adenocarcinoma

2021 ◽  
Vol 8 (3) ◽  
pp. 179-184
Author(s):  
Serkan Doğan ◽  
Emin Bağrıaçık ◽  
Şükran Sakaoğulları ◽  
Sedat Taştemur ◽  
Öner Odabaş
Keyword(s):  
Prostate Adenocarcinoma ◽  
Expression Level ◽  
Disease Stage ◽  
Immunohistochemical Expression ◽  
Pathological Disease

EXPRESSION LEVEL OF DRUG RESISTANCE GENES IN THE TUMOR OF OVARIAN CANCER PATIENTS AFTER NEOADJUVANT CHEMOTHERAPY

2019 ◽  
pp. 63-73
Author(s):  
L.V. Poludnyakova ◽  
D.R. Dolgova ◽  
I.I. Antoneeva ◽  
T.V. Abakumova ◽  
I.O. Kolodiy
Keyword(s):  
Ovarian Cancer ◽  
Drug Resistance ◽  
Neoadjuvant Chemotherapy ◽  
Resistance Genes ◽  
Expression Level ◽  
Ca 125 ◽  
Keywords Ovarian Cancer ◽  
Ercc1 Expression ◽  
Pathological Disease ◽  
Disease Parameters

The purpose of the paper is to evaluate the relationship between the expression of drug resistance genes in the tumor tissue of patients with ovarian cancer after neoadjuvant chemotherapy (NAChT) with clinical and pathological disease parameters. Materials and Methods. The study included 26 patients with ovarian cancer who had undergone NAChT according to the AP scheme. The authors used paired samples of normal and tumor ovatian tissue as material for their study. The expression level of ABCB1, ERCC1, and TOP2A genes was evaluated by real-time PCR. Results. It was found out that in patients under 60 years of age, the level of ABCB1 expression is higher than in patients over 60. ERCC1 and TOP2A expression in different age groups was the same. The linkage between the studied genes, tumor size and metastasis was not discovered. TOP2A and ABCB1 expression levels in patients with and without relapses did not differ significantly. In patients with relapses, the level of ERCC1 expression is higher than in patients without relapses. In patients with a normal CA-125 value, the level of ERCC1 expression is lower than in patients with a high CA-125 value. An analysis of the linkage between the expression of the studied genes and the general and relapse-free patient survival did not give significant results. Conclusion. The level of ABCB1expression is associated with the patients’ age, while the level of ERCC1expression is connected with disease recurrence and CA-125. In general, the expression of the studied genes weakly correlates with the main clinical and pathological disease parameters. Keywords: ovarian cancer, neoadjuvant chemotherapy, expression, drug resistance genes ABCB1, ERCC1, TOP2A. Цель работы – оценка связи экспрессии генов лекарственной устойчивости в опухолевой ткани больных раком яичников после проведения неоадъювантной химиотерапии (НАХТ) с клинико-патологическими параметрами заболевания. Материалы и методы. В исследование вошли 26 больных раком яичников после НАХТ по схеме АР. Материал для исследования – парные образцы нормальной и опухолевой ткани яичников. Уровень экспрессии генов ABCB1, ERCC1 и TOP2A оценивали при помощи ПЦР в реальном времени. Результаты. Выявлено, что у больных до 60 лет уровень экспрессии гена ABCB1 выше, чем у больных старше 60 лет. Экспрессия ERCC1 и TOP2A в разных возрастных группах не отличалась. Не установлена связь изучаемых генов с размером опухоли и метастазированием. Уровень экспрессии генов TOP2A и ABCB1 у больных с рецидивами и без них достоверно не отличался. У больных с рецидивами уровень экспрессии ERCC1 выше, чем у больных без рецидивов. У больных с нормальным значением СА-125 уровень экспрессии ERCC1 ниже, чем у больных с высоким показателем. Анализ связи экспрессии исследуемых генов с общей и безрецидивной выживаемостью больных не дал значимых результатов. Выводы. Уровень экспрессии гена ABCB1 связан с возрастом пациентов, а уровень экспрессии ERCC1 – с рецидивом заболевания и СА-125. В целом экспрессия изучаемых генов слабо коррелирует с основными клинико-патологическими параметрами заболевания. Ключевые слова: рак яичников, неоадъювантная химиотерапия, экспрессия, гены лекарственной устойчивости ABCB1, ERCC1, ТОР2А.

Correlative microscopy in distrubuted (client/server) computing environments: tools for catalyzing the rapid dissemination of information about the cell biology of the human prostate

1995 ◽  
Vol 53 ◽  
pp. 682-683
Author(s):  
A. Hakam ◽  
J.T. Gau ◽  
M.L. Grove ◽  
B.A. Evans ◽  
M. Shuman ◽  
...  
Keyword(s):  
United States ◽  
Cell Biology ◽  
Tissue Bank ◽  
The United States ◽  
Prostate Adenocarcinoma ◽  
Correlative Microscopy ◽  
Client Server ◽  
Critical Elements ◽  
Transplant Organ

Prostate adenocarcinoma is the most common malignant tumor of men in the United States and is the third leading cause of death in men. Despite attempts at early detection, there will be 244,000 new cases and 44,000 deaths from the disease in the United States in 1995. Therapeutic progress against this disease is hindered by an incomplete understanding of prostate epithelial cell biology, the availability of human tissues for in vitro experimentation, slow dissemination of information between prostate cancer research teams and the increasing pressure to “ stretch” research dollars at the same time staff reductions are occurring.To meet these challenges, we have used the correlative microscopy (CM) and client/server (C/S) computing to increase productivity while decreasing costs. Critical elements of our program are as follows:1) Establishing the Western Pennsylvania Genitourinary (GU) Tissue Bank which includes >100 prostates from patients with prostate adenocarcinoma as well as >20 normal prostates from transplant organ donors.

Automated Acoustic Analysis of Oral Diadochokinesis to Assess Bulbar Motor Involvement in Amyotrophic Lateral Sclerosis

2020 ◽  
Vol 63 (1) ◽  
pp. 59-73 ◽  
Author(s):  
Panying Rong
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Acoustic Analysis ◽  
Speaking Rate ◽  
Disease Stage ◽  
Healthy Controls ◽  
Tongue Movement ◽  
Major Barrier ◽  
Syllable Repetition ◽  
Oral Diadochokinesis ◽  
Lateral Sclerosis

Purpose The purpose of this article was to validate a novel acoustic analysis of oral diadochokinesis (DDK) in assessing bulbar motor involvement in amyotrophic lateral sclerosis (ALS). Method An automated acoustic DDK analysis was developed, which filtered out the voice features and extracted the envelope of the acoustic waveform reflecting the temporal pattern of syllable repetitions during an oral DDK task (i.e., repetitions of /tɑ/ at the maximum rate on 1 breath). Cycle-to-cycle temporal variability (cTV) of envelope fluctuations and syllable repetition rate (sylRate) were derived from the envelope and validated against 2 kinematic measures, which are tongue movement jitter (movJitter) and alternating tongue movement rate (AMR) during the DDK task, in 16 individuals with bulbar ALS and 18 healthy controls. After the validation, cTV, sylRate, movJitter, and AMR, along with an established clinical speech measure, that is, speaking rate (SR), were compared in their ability to (a) differentiate individuals with ALS from healthy controls and (b) detect early-stage bulbar declines in ALS. Results cTV and sylRate were significantly correlated with movJitter and AMR, respectively, across individuals with ALS and healthy controls, confirming the validity of the acoustic DDK analysis in extracting the temporal DDK pattern. Among all the acoustic and kinematic DDK measures, cTV showed the highest diagnostic accuracy (i.e., 0.87) with 80% sensitivity and 94% specificity in differentiating individuals with ALS from healthy controls, which outperformed the SR measure. Moreover, cTV showed a large increase during the early disease stage, which preceded the decline of SR. Conclusions This study provided preliminary validation of a novel automated acoustic DDK analysis in extracting a useful measure, namely, cTV, for early detection of bulbar ALS. This analysis overcame a major barrier in the existing acoustic DDK analysis, which is continuous voicing between syllables that interferes with syllable structures. This approach has potential clinical applications as a novel bulbar assessment.

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