scholarly journals Identification of bioactive compounds of the endophytic fungus Aspergillus egypticus-HT166S inhibiting the activity of pancreatic α-amylase

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Dilorom Ruzieva ◽  
Tashkan Gulyamova ◽  
Saodat Nasmetova ◽  
Iqbol Mukhammedov ◽  
Gulchehra Rasulova
Keyword(s):  
Bioactive Compounds ◽  
Endophytic Fungus

Bioactive compounds from an endophytic fungus from eastern larch (Larixlaricina) needles

1997 ◽  
Vol 75 (6) ◽  
pp. 716-719 ◽  
Author(s):  
John A. Findlay ◽  
Guoqiang Li ◽  
John A. Johnson
Keyword(s):  
Bioactive Compounds ◽  
Endophytic Fungus ◽  
Spruce Budworm ◽  
Insect Toxin

The structures of two novel metabolites isolated from cultures of an unidentified endophytic fungus obtained from an eastern larch (Larixlaricina (Du Roi) K. Koch) needle were shown to be 8,1′,5′-trihydroxy-3′,4′dihydro-1′H-[2,4′]binaphthalenyl-1,4,2′-trione, 1, and 2-methyloctanoic acid 6-oxo-2-propenyl-3,6-dihydro-2H-pyran-3-yl ester, 2. Structures were determined by spectroscopic means. Compound 1 was toxic to spruce budworm (ChoristoneurafumiferanaClem.) larvae while 2 was active against Vibriosalmonicida, Pseudomonasaeruginosa, and Staphylococcusaureus. Keywords: insect toxin, Larixlaricina endophyte, antimicrobial, endophyte metabolite.

scholarly journals Bioactive compounds from the endophytic fungus Fusarium proliferatum

2015 ◽  
Vol 30 (11) ◽  
pp. 1301-1304 ◽  
Author(s):  
Zerihun T. Dame ◽  
Beauty Silima ◽  
Marieka Gryzenhout ◽  
Teunis van Ree
Keyword(s):  
Bioactive Compounds ◽  
Endophytic Fungus ◽  
Fusarium Proliferatum

scholarly journals Bioactive Compounds from the Endophytes of Datura Metel L

2020 ◽  
pp. 160-169
Author(s):  
Madhu Priya M Ganesh ◽  
P Agastian
Keyword(s):  
Bioactive Compounds ◽  
Endophytic Fungus ◽  
Phenolic Content ◽  
Tropane Alkaloids ◽  
New Product ◽  
Spectroscopic Studies ◽  
Phytochemical Screening ◽  
Datura Metel ◽  
Ir Spectroscopic ◽  
Ethyl Acetate Extracts

The present study aims to investigate the preliminary phytochemical screening and various separation techniques to isolate the bioactive compounds from the endophytes of Datura metel. The ethyl acetate extracts of the endophytes are used for the current study. Phytochemical screening of the extracts revealed the presence of tannins, alkaloids, terpenoids and tropane alkaloids. The quantitative amounts of tropane alkaloids are significant in actinomycete (29.4%) than the endophytic fungus (23.2%). Actinomycete seems to contain higher phenolic content of 1. 271 mg/g whereas endophytic fungus accounts only 1.254 mg/g. The IR spectroscopic studies of endophytic fungus and actinomycetes showed peaks at different points and the results are noted. Hence, the isolation and production of bioactive compounds from the endophytes may facilitate the new product discovery process.

scholarly journals Bioactive Compounds from Aspergillus Terreus MP15, an Endophytic Fungus Isolated from Swietenia Macrophylla Leaf

2017 ◽  
Vol 4 (2) ◽  
pp. 107-116
Author(s):  
Olivia Chan Jade Yin ◽  
Darah Ibrahim ◽  
Chong Chai Lee
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungus ◽  
Aspergillus Terreus ◽  
Ethyl Acetate Extract ◽  
Bioactive Metabolites ◽  
Partial Purification ◽  
Swietenia Macrophylla ◽  
Gram Positive

Endophytes are important sources for the discovery of bioactive compounds. They are known as potential producers of novel compounds with antimicrobial activity. In this study, it aimed to study the bioactive metabolites present in the ethyl acetate extract from the endophytic fungus Aspergillus terreus MP15 of Swietenia macrophylla leaf. The ethyl acetate extract showed promising antibacterial activity against Gram-positive foodborne bacteria (Bacillus cereus, Bacillus spizizenii, Bacillus subtilis and Staphylococcus aureus) in disc diffusion assay. The extract was then subjected to thin layer chromatography (TLC) using dichloromethane: methanol (9:1) as a mobile solvent system and eleven spots with diverse polarities were obtained. The TLC chromatogram with bioactive spot was localized with bioautography assay and the result showed that the yellow spot with Rf value of 0.523 exhibited promising antibacterial activity against the four Gram-positive bacteria tested. Upon partial purification of the yellow bioactive fraction using column chromatography, the fraction 2a exhibited promising antibacterial activity. The GC-MS analysis of the yellow fraction 2a resulted in the detection of a major compound, di-n-octyl phthalate with 80% matching factor. Therefore, this compound may largely contribute to the antibacterial activity of the fraction and has potential to be food preservative and as a colorant. These results indicate that endophytic fungi isolated from medicinal plants could be a potential source for bioactive compounds.

Bioactive compounds of Aspergillus terreus—F7, an endophytic fungus from Hyptis suaveolens (L.) Poit

2017 ◽  
Vol 33 (3) ◽  
Author(s):  
Igor Pereira da Silva ◽  
Elson Brissow ◽  
Luis Claudio Kellner Filho ◽  
Jaqueline Senabio ◽  
Kátia Aparecida de Siqueira ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Endophytic Fungus ◽  
Aspergillus Terreus ◽  
Hyptis Suaveolens

Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.)

2021 ◽  
pp. 86-95
Author(s):  
Baiq Maylinda Gemantari ◽  
Fitra Romadhonsyah ◽  
Arief Nurrochmad ◽  
Subagus Wahyuono ◽  
Puji Astuti
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Endophytic Fungus ◽  
Fermentation Broth ◽  
Cytotoxic Activities ◽  
Dpph Assay ◽  
Nitrobenzyl Alcohol ◽  
Layer Chromatography ◽  
Coleus Amboinicus

Coleus amboinicus (Lour.) is a medicinal plant containing various bioactive compounds. Endophytes are microorganisms living inside intracellular tissue of plants and known as the source of bioactive compounds. In order to explore the potential of endophyte in producing novel bioactive compounds, this study focused on isolating endophytic fungi from the leaves of C.amboinicus, characterisation and screening their metabolite bioactivity during submerged culture fermentation. Isolation of endophytic fungi from the leaves segment of C.amboinicus was conducted on PDA media and fungus identification was carried out by analyzing its morphology and molecular examination. The production of metabolites was examined using thin layer chromatography and gas chromatography. Identification of the endophytic fungus showed 98.84% similarity with Eutypa linearis. This species was fermented submergedly in PDB medium for 14 days under dark and exposed to light and the fermentation broth was extracted using ethyl acetate. The results showed that exposure to the light did not significantly influence metabolite production. The ethyl acetate extract exhibited antioxidant and cytotoxic activities. Antioxidant activity of this extract as examined by DPPH assay showed IC50 of 105.31 ± 2.11 µg/mL. Cytotoxic activity against Hela cell line was known to be the best among other cell lines (IC50 301.53 ± 11.34 µg/mL) although it was found to be non selective (SI<1). The extract contained five major compounds namely Benzenemethanol, 4-nitro-(CAS) p-Nitrobenzyl alcohol; 2-Pentadecanone (CAS) Pentadecan-2-one; (1R*,6S*,10R*)-5,5-Dimethyl-11,12-dioxatricyclo[8.2.1.0(1,6)] tridecan-10-ol; 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (CAS) Methyl linoleate; and 3-Furanacetic acid, 4-hexyl-2,5-dihydro-2,5-dioxo- (CAS) 2-carboxymethyl-3-N-hexyl-maleic anhydride.

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